Baker Invivo2 UM-014 User manual
UM-014
Invivo2Plus User Manual
Affix Serial Number Sticker Here
Invivo2 Plus
UM-14 Version R3.0 Page 2 Invivo2 Plus User Manual
Product Summary
Baker Ruskinn's Invivo2hypoxia workstation allows you to isolate cells in a chamber that accurately
maintains and controls temperature, humidity, oxygen and carbon dioxide. With the Invivo2, you can
study even the most complex cell interactions under perfect microaerophilic or anaerobic
conditions.
Features and Benefits
•Quick and easy direct access with the gloveless, cuffed Ezeeyin Sleeve system.
•Single plate entry system (SPES) - standard on most models, this mailbox-like slot allows
quick side entry or exit of individual plates, bypassing the interlock cycling process.
•Read plates easily without exposure to oxygen - energy-saving lighting provides perfect
illumination.
•Up to 520 90mm plate capacity
•Interlock transfer 78 plates in 5 minutes.
•Utilised with the ICO2N2IC Advanced Gas mixing system to offer a microaerophilic
environment in which to grow facultative anaerobes.
•Optimum cell environment
oAccurate temperature control - from ambient + 5˚C to 45˚C.
oAccurate and automated humidity control - no dry spots.
oPalladium catalyst maintains anaerobic environment as an optional extra with
anaerobic colour-indicator strips verifying anaerobic conditions.
oEzeeyin Sleeve system allows access without disrupting atmosphere within the
chamber.
•Economic and reliable long-term savings
oStandard dual gas operation - low gas consumption and running costs.
oMinimal maintenance and downtime - with annual or biennial preventative
maintenance kits available.
The ICO2N2IC provides accurate control over O2(0.1% - 20.9% in 0.1% increments, can reach 23.0%
using separate 25% O2cylinder) and CO2(0.1% - 30% in 0.1% increments).
The ICO2N2IC is available in two options.
•Hypoxic (standard build)
•Anaerobic (optional accessory)
*Note. The use of the word Interlock in this manual is medical terminology, and refers to the
environmental working area between both chambers. It does not refer to the engineering safety
interlock switch mechanism.
Invivo2 Plus
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Table of Contents
Product Summary ...................................................................................................................................2
Features and Benefits .............................................................................................................................2
Overview and Safety Instructions..............................................................................................61.
Regulatory compliance .................................................................................................71.1
Symbols.........................................................................................................................71.2
Installation and relocation............................................................................................81.3
Weight and dimensions ................................................................................................ 91.4
Gas and electrical supply requirements ..................................................................................102.
Gas supply requirements ............................................................................................102.1
Gas regulator requirements........................................................................................ 102.2
Electrical supply requirements ...................................................................................102.3
Voltage and frequency requirements.........................................................................102.4
Power consumption....................................................................................................102.5
Powering the workstation ..........................................................................................112.6
Workstation overview..............................................................................................................123.
Workstation layout .....................................................................................................123.1
Component layout ......................................................................................................123.2
Front view .......................................................................................................123.2.1
Rear view ........................................................................................................133.2.2
Left side view – Standard humidifier .............................................................. 143.2.3
Left side view – Ultrasonic humidifier ............................................................153.2.4
Right side view ................................................................................................ 163.2.5
Control system layout.................................................................................................173.3
Control panel – Standard humidity control .................................................... 173.3.1
Control panel – Ultrasonic humidity control .................................................. 183.3.2
Temperature controller / ultrasonic humidity controller...............................193.3.3
Interlock internal door control panel .............................................................203.3.4
Interlock outer door control panel ................................................................. 213.3.5
Foot switch control panel ...............................................................................213.3.6
Power entry panel .......................................................................................... 223.3.7
Temperature and humidity control ......................................................................................... 234.
Temperature control...................................................................................................234.1
Humidity control ......................................................................................................... 234.2
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Standard humidity control..............................................................................234.2.1
Environmental gas composition control..................................................................................235.
Using the workstation chamber...............................................................................................256.
Using the interlock...................................................................................................... 256.1
Interlock overview ..........................................................................................256.1.1
Opening the interlock outer door...................................................................256.1.2
Opening the interlock inner door ................................................................... 256.1.3
Transferring material into the workstation chamber via the interlock..........256.1.4
Removing material from the workstation chamber via the interlock ............ 266.1.5
Hand access to the main chamber..............................................................................266.2
Ezeeyin Sleeve System Overview....................................................................266.2.1
Vacuum Operation.......................................................................................... 276.2.2
Workstation Entry...........................................................................................296.2.3
Workstation Exit .........................................................................................................326.3
Single Plate Entry System (SPES).................................................................................336.4
SPES overview.................................................................................................336.4.1
Using the SPES ................................................................................................346.4.2
Internal power socket.................................................................................................356.5
Internal lights ..............................................................................................................356.6
Rear shelf .................................................................................................................... 356.7
Optional accessories ...................................................................................................366.8
Cable gland .....................................................................................................366.8.1
Universal cable gland......................................................................................366.8.2
Gas sample port..............................................................................................376.8.3
Vacuum port ...................................................................................................376.8.4
Online video user guides............................................................................................. 396.9
Cleaning and maintenance ......................................................................................................407.
Cleaning the workstation............................................................................................407.1
Cleaning agents...............................................................................................407.1.1
Cleaning procedure – during and after each use............................................407.1.2
Removing/ reinstalling the front screen.....................................................................417.2
Cleaning procedure – deep clean ...............................................................................417.3
Maintaining the workstation – End user maintenance ..............................................437.4
Filling the pressure relief/ humidifier tank .....................................................437.4.1
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Replacing an Ezee Sleeve................................................................................ 447.4.2
Replacing detox sachets ................................................................................. 447.4.3
Installing/ replacing a catalyst sachet............................................................. 467.4.4
Replacing the mains plug fuse – UK users only .............................................. 487.4.5
Replacing the mains fuses .............................................................................. 497.4.6
Service requirements..................................................................................................507.5
Spare parts and accessories........................................................................................507.6
Overview.........................................................................................................507.6.1
Spare parts and cleaning agents.....................................................................517.6.2
Accessories......................................................................................................527.6.3
Workstation malfunction............................................................................................527.7
Common problems and solutions...............................................................................537.8
Workstation general problems.......................................................................537.8.1
Interlock problems..........................................................................................547.8.2
Warranty information..............................................................................................................558.
Disposal information................................................................................................................569.
Contact details .........................................................................................................................5710.
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Overview and Safety Instructions1.
Please read this manual carefully to familiarise yourself with the operation and maintenance of your
Invivo2 Plus workstation. Note: The workstation should be located in a well-ventilated area.
For your safety, safety of others using the workstation and those around you;
•The covers on both ends and the top of the workstation must not be removed by anyone other
than a qualified service engineer. There are no end user serviceable parts within these covers.
•The AC Mains outlet that the Invivo2Plus workstation is connected to, MUST not be
obstructed by the Invivo2Plus workstation or any other equipment, and MUST be accessible in
case of emergency. In case of emergency, disconnect the Invivo2Plus workstation from the AC
Mains Outlet.
•In case of damage to the Invivo2Plus workstation, disconnect the Invivo2Plus workstation
from the AC Mains Outlet and contact your local distributor for advice.
•The workstation must be connected to a protective earth.
•Only the power cord supplied with the workstation should be used to connect the workstation
to the mains supply.
•Only Ruskinn Technology Limited replacement parts should be used.
•Gas regulators must be used for each gas supply. A 2 stage regulator should be used for a
bottled gas supply. The maximum supply pressure permissible is 4 bar gauge.
•The maximum permissible concentration of Hydrogen in the anaerobic mixed gas is 5.5%
Hydrogen.
•The mains supply voltage fluctuations must not exceed +/- 10% of the nominal mains voltage.
•Only the gasses specified in this manual may be used.
•The maximum power ratings of the internal sockets must not be exceeded.
•The exhaust valve outlet must not be covered or blocked.
•The cooling fan covers and vents must not be covered or blocked.
•The workstation must be disconnected from the mains supply before removing the front
screen. The workstation must not be reconnected to the mains supply until the front screen
has been reinstalled.
•The workstation should not be lifted by the glove ports, the interlock or the Single Plate Entry
System.
•If an Uninterruptable Power Supply (UPS) system is used, both the Invivo2Plus workstation
and the ICO2N2IC Advanced Gas mixing system must be connected to the Uninterruptable
Power Supply (UPS) system.
•The weight limit for the interlock floor tray is 4kg.
•The weight limit for the rear shelf is 12.5kg, evenly distributed.
•The spot light should not be used continuously for a period of more than 10 minute. The spot
light should be allowed to cool for a period of 10 minutes after each use.
•The use of Radioactive materials in the Invivo2400 is strictly prohibited.
FAILURE TO ADHERE TO THESE SAFETY INSTRUCTIONS COULD CAUSE SERIOUS INJURY AND WILL
INVALIDATE THE WORKSTATION WARRANTY. RUSKINN TECHNOLOGY LIMITED ACCEPTS NO
RESPONSIBILITY FOR ANY ACCIDENT, INJURY OR LOSS CAUSED BY UNSAFE OPERATION OF THE
WORKSTATION
Invivo2 Plus
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Regulatory compliance1.1
This product complies with the essential EEA requirements for Electrical Safety and the Low Voltage
Directive 2006/95/EC as well as Electromagnetic compatibility as set out in the EMC Directive
204/108/EC.
Symbols1.2
Before using the Invivo2 Plus, please ensure that you are familiar with the symbols on the Invivo2
Plus. Figure 1 explains the symbols found on the Invivo2 Plus;
Figure 1: Invivo2 Plus symbols
Symbol
Meaning
Refer to user manual.
~
Alternating current
O
Off
I
On
Primary earth connection
This product complies with the essential EEA
requirements for Electrical Safety and
Electromagnetic compatibility as set out in
the EMC directive 2004/108/EC and the Low
Voltage Directive 2006/95/EC
Caution, do not remove covers. No end user
serviceable parts behind covers. Please refer
to this manual in all cases where this symbol
appears, in order to find out the nature of
the Potential Hazard and actions to be taken
in order to avoid the Hazard.
Symbol
Meaning
Invivo2 Plus
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Warning, this equipment contains high
voltage circuitry.
Warning:
Do not use toxic or Flammable substances
inside the incubator.
Invivo
2
Plus contains hazardous components
and must not be disposed of at a household
waste site. Instead it should be taken to the
appropriate collection point for the recycling
of electrical and electronic equipment.
Date of manufacture in format
YYYY MM
Installation and relocation1.3
Invivo2 Plus should only be installed or relocated by a qualified engineer. To arrange installation or
relocation, please contact your local distributor.
The mains socket that forms connection to the Invivo2Plus, is not to be obstructed by
Invivo2Plus or any other equipment, and must be accessible in case of emergency. In case
of emergency, disconnect the Invivo2Plus from the AC mains outlet.
Invivo2 Plus
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Weight and dimensions1.4
The Invivo2 Plus workstations weigh approximately 130kg. Figure 2 lists the dimensions of the Invivo2
Plus workstation;
Figure 2: Invivo2 Plus workstation dimensions
External width
1586 mm
External height
725 mm
External depth
714 mm
Workstation chamber internal width
1050 mm
Workstation chamber internal height
479 mm
Workstation chamber internal depth
575 mm
Interlock internal width
320 mm
Interlock internal height
300 mm
Interlock internal depth
314 mm
Interlock outer door clear opening width
300 mm
Interlock outer door clear opening height
250 mm
Interlock inner door clear opening width
230 mm
Interlock inner door clear opening height
255 mm
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Gas and electrical supply requirements2. Gas supply requirements2.1
The Invivo2 Plus workstation requires;
•Oxygen free nitrogen.
•In addition to this, the Invivo2Plus workstation should be connected to ICO2N2IC (supplied
with the Invivo2Plus workstation). All other gas connections should be made to the
ICO2N2IC.
See UM-011 ICO2N2IC user manual for more details.
The maximum permissible concentration of hydrogen in the anaerobic mixed gas is 5.5%. The use
of any other gas(ses) will invalidate the warranty and may cause serious injury. The Gas supplies
must be securely fastened after installation.
Gas regulator requirements2.2
Gas regulators must be used with all gas supplies to the workstation. A 2 stage regulator must be
used for each bottled gas supply. Regulators should be available from your local gas supplier.
Ruskinn Technology Limited does not supply gas regulators.
The gas regulator should provide a minimum supply pressure of 3 (42psi) Bar Gauge. The maximum
supply pressure permissible is 4 bar gauge. A supply pressure greater than this will damage internal
components of the workstation and will invalidate the warranty.
The gasses are connected to the ICO2N2IC Advanced Gas mixing system. See UM-011 ICO2N2IC user
manual for more details.
Electrical supply requirements2.3
The workstation must be connected to a mains power supply. A power cord is supplied to connect
the workstation to the mains supply. Only the power cord supplied should be used to connect the
workstation to the mains supply. It is advised that the workstation be located no greater than 1
metre from the plug socket. The plug socket should not be obscured by the Invivo2Plus workstation.
The workstation must be connected to a protective earth.
NOTE: If an Uninterruptable Power Supply (UPS) system is used, both the Invivo2Plus workstation
and the ICO2N2IC Advanced Gas mixing system must be connected to the Uninterruptable Power
Supply (UPS) system.
Voltage and frequency requirements2.4
To ensure safe operation of the workstation, it must be connected to a supply of the correct voltage
and frequency, as shown in the rating label (Figure 8). The mains supply voltage fluctuations must
not exceed +/- 10% of the nominal mains voltage.
Power consumption2.5
Figure 3: Power consumption ratings
Supply voltage and frequency
Power consumption
240V AC, 50Hz
200W
120V AC, 60Hz
250W
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Note: The power consumption is for normal operating conditions with no equipment connected to
the internal power supply. Power consumption will vary dependent upon the conditions inside the
workstation chamber and the conditions inside the room the workstation is located in.
Powering the workstation2.6
To switch the workstation on, press the power switch (item 6 in Figure 15).
To switch the workstation off, press the power switch (item 6 in Figure 15).
Note: A glove port should be left open when the workstation is switched off, as the pressure inside
the workstation will decrease as the workstation cools. The open glove port will allow the pressure
inside the workstation chamber to equalise with the external pressure.
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Workstation overview3. Workstation layout3.1
The Invivo2 Plus workstation consists of 2 main areas; the workstation chamber and the interlock
chamber. The workstation chamber is the main working area of the workstation. Access to the
workstation chamber is provided by the glove ports, the Single Plate Entry System (SPES) and the
interlock. The interlock consists of the interlock chamber, with an inner and an outer door. The
Invivo2Plus workstation is controlled via the control panel. The ICO2N2IC Advanced Gas mixing
system controls the gaseous composition of the internal environment. See UM-011 for more
information on the ICO2N2IC Advanced Gas mixing system.
Component layout3.2
Please familiarise yourself with the layout of your Invivo2Plus workstation.
Front view3.2.1
Figure 4 shows the front view of the Invivo2 Plus workstation;
Figure 4: Invivo2 Plus workstation front view
1. Front screen knobs.
2. Glove ports (shown without Ezee Sleeves).
3. Rear shelf.
4. Interlock internal door control panel.
5. Control panel.
6. Interlock outer door.
7. Interlock outer door control panel.
2
1
3
4
5
6
7
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Left side view – Standard humidifier3.2.3
Figure 6 shows the left side view of the Invivo2 Plus workstation with the standard humidifier;
Figure 6: Invivo2 Plus workstation left side view with standard humidifier
1. Exhaust valve outlet.
2. Condenser fan.
3. Pressure relief tank cover (pressure relief tank bung).
4. Pressure relief tank.
5. Vacuum line (optional accessory).
6. Pressure relief tank overflow tube.
7. Single Plate Entry System (SPES).
3
1
4
5
6
7
2
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Left side view – Ultrasonic humidifier3.2.4
Figure 7 shows the left side view of the Invivo2 Plus workstation with the ultrasonic humidifier;
Figure 7: Invivo2Plus workstation left side view with ultrasonic humidifier
1. Exhaust valve outlet.
2. Condenser fan
3. Cable gland (optional accessory).
4. Humidifier tank.
5. Humidifier tank filler cap.
6. Vacuum line (optional accessory).
7. Single Plate Entry System (SPES).
8. Tank Plug
3
1
4
5
6
7
2
8
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Right side view3.2.5
Figure 8 shows the right side view of the Invivo2 Plus workstation;
Figure 8: Invivo2Plus workstation right side view
1. Serial number /Rating label.
2. Portable appliance test (PAT test) label.
3. Mixed gas inlet.
4. Gas sample return.
5. Gas sample out.
6. Power entry panel.
1
2
3
4
5
6
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Control system layout3.3
The control system layout varies by model. Please familiarise yourself with the control system layout
of the Invivo2 Plus workstation.
Control panel – Standard humidity control3.3.1
Figure 9 shows the control panel of Invivo2 Plus workstations equipped with standard humidity
control;
Figure 9: Invivo2Plus workstation control panel
1. Power indicator. Illuminates to indicate the workstation is connected to the mains power
supply and is switched on.
2. Chamber light switch. Press to switch the chamber light on / off. The chamber light switches
off after 1 hour automatically.
3. Internal socket. Press to switch the internal socket on / off.
4. Temperature controller. See section 3.3.3, for more details.
5. ANO2low.
6. N2low.
7. Gas alarm. Press to switch the gas alarm on / off.
8. Com. Cycle active.
9. Interlock purge cycle selector. Press to switch between the standard (3mins) and economy
(2minutes) interlock cycles. When the switch is depressed, the economy cycle is selected.
Note that this feature is an optional accessory.
10. Interlock cycle status aerobic indicator.Illuminates to indicate that the interlock chamber is
in an aerobic condition and requires purging
11. Interlock cycle status purge indicator. Illuminates to indicate that the interlock purge cycle
is in progress.
12. Interlock cycle status anaerobic indicator.Illuminates to indicate that the interlock chamber
is in an anaerobic condition.
13. Inner door indicator. Illuminates to indicate that the interlock inner door is open.
14. Outer door indicator. Illuminates to indicate that the interlock outer door is open.
15. Gas Excess.
16. Gas Demand. Illuminates to indicate a low pressure condition inside the workstation
chamber.
17. Gas change
18. Humidistat. See Sector 4.2 for more details.
1
2
3
4
5
6
7
9
10
8
15
16
14
13
12
11
17
18
19
20
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19. Socket on indicator. Illuminates to indicate that the internal socket is switched on.
20. Condenser fan indicator. Illuminates to indicate the condenser fan is running.
Control panel – Ultrasonic humidity control3.3.2
Ultrasonic humidity control is available as an optional accessory on the Invivo2 Plus. Ultrasonic
humidity is only available at time of order and is not available as an aftermarket upgrade. Figure 10
shows the control panel for Invivo2 Plus workstations equipped with ultrasonic humidity;
Figure 10: Control panel for Invivo2Plus workstations with ultrasonic humidity
1. Power indicator. Illuminates to indicate the workstation is connected to the mains power
supply and is switched on.
2. Chamber light switch. Press to switch the chamber light on / off. The chamber light switches
off after 1 hour automatically.
3. Internal socket switch.Press to switch the internal socket on / off.
4. Temperature controller. See section 3.3.3, for more details.
5. ANO2low.
6. Gas alarm. Press to switch the gas alarm on / off.
7. N2low.
8. Com. Cycle active.
9. Interlock purge cycle selector. Press to switch between the standard and economy interlock
cycles. When the switch is depressed, the economy cycle is selected.
10. Condenser fan on. Illuminates to indicate the condenser fan is running.
11. Socket on. Illuminates to indicate that the internal socket is switched on.
12. Humidity controller. See section 3.3.3, for more details.
13. Gas change.
14. Gas demand. Illuminates to indicate that gas is being injected into the workstation from the
ICO2N2IC Advanced gas mixer system.
15. Gas Excess.
16. Inner door indicator. Illuminates to indicate that the interlock inner door is open.
17. Outer door indicator. Illuminates to indicate that the interlock outer door is open.
18. Interlock cycle status aerobic. Illuminates to indicate that the interlock chamber is in an
aerobic condition and requires purging.
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19. Interlock cycle status purge. Illuminates to indicate that the interlock purge cycle is in
progress.
20. Interlock cycle status anaerobic. Illuminates to indicate that the interlock chamber is in an
anaerobic condition.
Note:For both control panels, the ANO2, Com. Cycle active, gas change and gas excess indicators are
not used by the Invivo2 Plus workstation. The functions are used by the Concept Plus workstation,
which shares the same control panel as the Invivo2Plus workstation. LEDs are provided to blank
holes in the control panel.
Temperature controller / ultrasonic humidity controller3.3.3
Figure 11 shows the temperature / ultrasonic humidity controller;
Figure 11: Temperature / ultrasonic humidity controller
1. Not user accessible (For service personnel only)
2. Not user accessible (For service personnel only)
3. Number scrolling button. Use to move across digits. Use with buttons 4
& 5 to increase and decrease the temperature.
4. Decrease temperature (0.1°C increments) or humidity.
5. Increase temperature (0.1°C increments) or humidity.
For temperature control, the white value (PV- Process Value) is the actual temperature. The green
value (SV- Set Value) is the required value (the set point). All temperatures are in °C.
For ultrasonic humidity control, the white value is the actual relative humidity and the green value
the required relative humidity (the set point). All humidity’s are percentage relative humidity.
1
2
3
4
5
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Interlock internal door control panel3.3.4
Figure 12 shows the interlock internal door control panel;
Figure 12: Interlock internal door control panel
1. Inner door LED indicator. Illuminates to indicate when the interlock inner door is available.
2. Inner door button. Press to unlock the interlock inner door (when available).
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