BIO RAD NGC User manual

2Connect the power cable and USB to the back of the FC.
Plug in the power supply and connect the USB cable into
a USB port on the NGC Chromatography System.
Introduction
The NGC Fraction Collector provides versatile options for
preparative and analytical chromatography applications. The
high-capacity and flexible rack options enable fractions ranging
in size from milliliters to liters to be collected and accessed
freely during a purification run.
Setting Up the NGC Fraction Collector
This quick guide outlines the process of installing and plumbing
the NGC Fraction Collector to your NGC System. The fraction
collector (FC) can be installed on a laboratory bench or in a
coldroom or chromatography refrigerator.
1Open the shipping box and remove the foam packaging.
Carefully lift the FC from the box. Do not lift the FC by the
gantry; reach down and lift it from the base. Grip the base
of the FC by the sides to lift from the shipping box.
Check the box for the tubing kit, external power supply,
and USB cable. The tubing kit contains:
 92 cm green polyetheretherketone (PEEK) tubing
from NGC union to dispense head for F100 systems
(fittings on both ends)
 92 cm orange PEEK tubing for F10 systems (fittings
on both ends)
 92 cm clear fluorinated ethylene propylene (FEP)
waste line from dispense head to union (fittings on
both ends)
 150 cm clear FEP waste line from union to waste
bottle (fitting on one end)
 Black magnetic union to be attached to side of NGC
System to act as anchor point for FC tubing; position
as necessary to avoid kinking any of the tubes
Installing the NGC Fraction Collector
CHROMATOGRAPHY SYSTEMS
NGC™Fraction Collector Installation
Quick Guide
Dispense head height adjustment
Standard racks
USB port Power port
Drip tray
Dispense head cover
Gantry

Plumbing the NGC Fraction Collector
Use the supplied tubing and union to plumb from the NGC System
to the dispense head on the fraction collector. Plumb the waste line
from the dispense head to the waste bottle via the union.
1Open the dispense head cover using a small
Phillips screwdriver.
2Connect the sample tube to the dispense head. Use the
cutout in the back of the cover to insert a fittings tightener
and tighten.
3Attach the waste line from the dispense head waste port to
the union on the side of the NGC System.
4Route both tubes through the top of the dispense head
cover by sliding them into the rubber grommet. Finally, close
the dispense head cover and fasten with the screw.
5Attach the tubes to the union so the direction of flow is as
shown below.
Sample tube
To FC
inlet port
From NGC
From FC
waste port
To waste
bottle
Dispense head
waste port
Dispense head
cover
Waste line
Rubber
grommet
Cutout
Dispense head

Completing NGC Fraction Collector Installation
After plumbing the NGC Fraction Collector, complete the
installation and adjustments.
1Place the plastic drip tray on the FC with the notched corner
facing the left rear of the FC platform.
2Place the metal rack tray into position, ensuring it is aligned
correctly on the locating pins.
3Turn on the fraction collector using the power button on
the front.
4The gantry and dispense head will move to the home
position when the NGC is powered up and the fraction
collector is detected by the system.
Note: The gantry will move quickly from the home position
to the first collection tube at the start of a run. Ensure there
are no objects in the way before starting collection.
5Place the racks on the FC, ensuring they are correctly
aligned on the raised locating pins. Identifier labels on the
racks should face right.
6Before using the fraction collector, adjust the z-height of the
dispense head to sit within 10 mm of the collection vessels.

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Bio-Rad
Laboratories, Inc.
Life Science
Group
PEEK is a trademark of Victrex plc. Phillips is a trademark of Phillips Screw Co.
Ordering Information
Catalog # Description
12 0 0 374 8 Peltier Rack Power Supply, 4-channel power supply for up to
4 Peltier Rack Modules
12 0 0 374 9 Peltier Rack Module, 1 insulated rack per module
12003759 Insulated Rack for 50 ml Tubes, for 14 x 50 ml tubes
12003751 Insulated Rack for 15 ml Tubes, for 30 x 15 ml tubes
12 0 0 3747 Peltier Rack Module Microtiter Plate Adaptor, for 2 deep well microtiter plates
12003752 Rack for 13 mm Tubes, for 96 x 13 mm tubes
12003755 Rack for 16 mm Tubes, for 75 x 16 mm tubes
12003756 Rack for 18 mm/15 ml Tubes, for 70 x 18 mm tubes
12003757 Rack for 50 ml Tubes, for 27 x 29 mm tubes
12003754 Rack for Deep Well Microtiter Plates, for 2 x 24-, 48-, or 96-well plates
12003753 Rack for 1.5–2 ml Capless Microtubes, for 96 tubes
12003760 Plate Rack Riser
Visit bio-rad.com/NGCFC for more information.