BIO RAD Experion DNA 12K analysis kits User manual

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Sciences, Inc. Bio-Rad Laboratories, Inc. is licensed by Caliper Life
Sciences, Inc. to sell products using the LabChip technology for
research use only. These products are licensed under U.S. Patent
Nos. 5,863,753, 5,658,751, 5,436,134, and 5,582,977, and pending patent
applications, and related foreign patents, for internal research and development
use only in detecting, quantitating, and sizing macromolecules, in combination
with microfluidics, where internal research and development use expressly
excludes the use of this product for providing medical, diagnostic, or any other
testing, analysis, or screening services, or providing clinical information or
clinical analysis, in any event in return for compensation by an unrelated party.
Experion™DNA 12K Analysis Kit
Quick Guide
30 40 50 60 70 80 90 100
Time, sec
Fluorescence
DNA 12K Ladder
350
300
250
200
150
100
50
0
DNA 1K = C3
12K = C1
LOT# J M27UP 02
EX P AP R/2 5/08
LOT# J J29UK 01
EX P MA R/ 31/0 8
LOT# KA 31U K0 1
EX P MAY/0 1/0 8
LOT# J J05UK 02
EX P DEC /05/07
Storage Conditions
Product Storage
DNA 12K chips Room temperature
DNA 12K reagents 4oC (protected from light)
Essential Practices
nAlways wear gloves when handling reagents and chips
nHandle chips by the edges; do not touch the glass
nRemove chip from packaging only immediately before use
nAvoid sources of dust and contaminants when preparing
samples and loading the chip. Foreign particles in reagents,
samples, and the wells of the chip can interfere with results
nDeep-clean the electrodes if contamination is suspected or
if a chip was left in the instrument overnight
nUse of colored or coated (for example, siliconized
polypropylene) tubes when preparing kit reagents or samples
is not recommended; such tubes may cause artifacts during
the separation
nRefer to the instruction manual (Bulletin 10006453) for
more details
Life Science
Group
09-0125 0210 Sig 1109
10006455 Rev B US/EG
Bio-Rad
Laboratories, Inc.
Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 31 3689 6600
Canada 905 364 3435 China 86 20 8732 2339 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00 France 01 47 95 69 65
Germany 089 31 884 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300 Israel 03 963 6050
Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666 New Zealand 0508 805 500
Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04 Singapore 65 6415 3188 South Africa 27 861 246 723
Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland 061 717 95 55 Taiwan 886 2 2578 7189 United Kingdom 020 8328 2000
Ordering Information
Catalog # Description
700-7001 Experion System, 100–240 V,for RNA and DNA analyses,
includes electrophoresis station, priming station, vortex
station, software, USB2 cable
700-7108 Experion DNA 12K Analysis Kit for 10 Chips, includes
10 DNA chips, 1 cleaning chip, 650 µl DNA 12K gel, 40 µl
DNA 12K stain, 20 µl DNA 12K ladder, 750 µl DNA 12K loading
buffer, 3 spin filters
700-7163 Experion DNA Chips, 10, for DNA 1K and 12K analyses,
plus 1 cleaning chip
700-7165 Experion DNA 12K Reagents and Supplies for 10 Chips,
includes 650 µl DNA 12K gel, 40 µl DNA 12K stain, 20 µl DNA
12K ladder, 750 µl DNA 12K loading buffer, 3 spin filters
700-7253 Experion DEPC-Treated Water,100 ml
700-7262 Experion DNA 12K Ladder,20 µl
700-7251 Experion Cleaning Chips,10
700-7254 Experion Spin Filters,10
700-7264 Cleaning Swabs, lint-free, for Experion system electrode
deep cleaning, 25
700-7252 Experion Electrode Cleaner, 250 ml
732-6300 PCR Kleen Spin Columns, 25
732-6223 Micro Bio-Spin 30 Columns, includes 25 columns in
Tris buffer, 50 collection tubes
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Experion DNA 12K Analysis Kit Quick Guide
For complete instructions, refer to the Experion DNA analysis kit instruction manual. Full manuals are available online at www.bio-rad.com. You can contact us by
phone at 1-800-424-6723 or call your local Bio-Rad office for an electronic copy. Read the full protocol and essential practices sections if using for the first time.
n
Equilibrate reagents to
room temperature (~15 min).
Protect them from light
Note: Store remaining G from stock tube at 4˚C. After use, store filtered GS
(protected from light) at 4˚C. Discard any remaining filtered GS after 1 month.
Do not refilter.
Prepare Gel-Stain (GS) Solution
GG ST LB ST
10 μl
200 μl
1
6 7
nBriefly vortex and spin down reagents
Run the DNA 12K Analysis
nSelect New Run, then DNA 12K assay
nClick the Start ( ) button onscreen
n Select number of samples to run and start
nWhen the run is complete, remove and
discard the used chip
DNA 1K = C3
12K = C1
LOT# J M27UP 02
EX P APR/ 25/08
LOT#JJ29UK01
EXPMAR/31/08
LO T# K A 3 1U K 0 1
EX P MAY/0 1/08
LOT# J J05UK 02
EX P DEC/0 5/07
Clean the Electrodes
Note: If using the instrument for DNA analysis after a protein run, use the deep-cleaning procedure instructions
in the DNA or RNA analysis kit manual or the software version ≥ 3.0 Help section (use search term: electrodes).
Equilibrate Kit Reagents (ST = stain, B = loading buffer,
L = ladder, G = gel) 2Prepare DNA Samples (If Needed)
nIf samples are very concentrated, dilute (with TE buffer or DNase-free
water) to approximately 50 ng/µl. Buffer exchange or clean up
samples (for example, using PCR clean up kits) if samples are in
salt concentrations ≥ 200 mM KCl (or NaCl) and 15 mM MgCl2
3
Cleaning chip
+800 µl
DNase-
free
water
60 sec60 sec
H20
5
DNA 1K = C3
12K = C1
LOT# J M27UP 02
EX P APR /25/0 8
LOT# J J29UK01
EX P MAR /31/08
LOT# K A 31UK 01
EX P M AY/01/0 8
LOT# J J0 5UK02
EX P DE C/05/ 07
DNA 1K = C3
12K = C1
LOT# J M27UP 02
EX P APR /25/0 8
LOT# J J29UK01
EX P MAR /31/08
LOT# K A 31UK 01
EX P M AY/01/0 8
LOT# J J0 5UK02
EX P DE C/05/ 07
DNA 1K = C3
12K = C1
LOT# J M27UP 02
EX P APR /25/0 8
LOT# J J29UK01
EX P MAR /31/08
LOT# K A 31UK 01
EX P M AY/01/0 8
LOT# J J0 5UK02
EX P DE C/05/ 07
9 µl
GS
1 µl
L
5 µl
B
5.1 5.2 5.3 5.4 5.5
n 
Pipet 9 µl filtered
GS solution into
other 3 GS wells
n
Pipet 5 µl loading
buffer into the Lwell
and each sample
well (1–11); do not
leave any well empty
n
Pipet 1 µl DNA
12K ladder into
well L
n
Pipet 1 µl of DNA
sample into each of
the 11 sample wells.
Pipet 1 µl of TE or
DNase-free water into
any unused wells
n 
Place the chip in the
Experion vortex station and
vortex (press Mix; vortex
station automatically
stops after 1 min)
n 
Run the chip in the
Experion electrophoresis
station within 5 min
of loading
Load the Prepared Samples and DNA 12K Ladder Onto the Chip
1 min
1 µl
Sample
DNA 1K = C3
12K = C1
LOT# J M27UP 02
EX P APR /25/0 8
LOT# J J29UK01
EX P MAR /31/08
LOT# K A 31UK 01
EX P M AY/01/0 8
LOT# J J0 5UK02
EX P DE C/05/ 07
GS
4
C1
9 µl
DNA 1K = C3
12K = C1
LOT# J M27UP02
EX P APR /2 5/08
LOT# J J29U K01
EX P MAR /31/08
LOT# K A 31UK01
EXP M AY/01/08
LOT# J J0 5U K02
EX P DE C/05/07
Gel
priming
well
GS
Prime the Chip
n 
Add GS to priming well
n Select C1 on priming station
n 
Place chip in station and press Start
n Remove chip after priming is complete
n Flip chip over and visually inspect the microchannels
for trapped air bubbles or incomplete priming
10 sec
GS
transfer all
n 1,500 x g for 10 min
n Then discard filter
spin filter
tube
GS
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