Thermo Scientific Sorvall BIOS A User manual

Thermo Scientific
Sorvall BIOS A
Centrifuge and Heavy Duty Centrifuge
Instruction Manual
50154616-c • 03 / 2021
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Thermo Scientific Sorvall BIOS A Centrifuges
Table of Contents
Preface .................................................vi
Items Supplied ....................................................vi
Intended Use for Sorvall BIOS A and Sorvall BIOS A Heavy Duty ...........vii
Associated Rotors and Accessories.........................................................................................vii
Precautions......................................................vii
Set Up Conditions.................................................................................................................. vii
Preparation .......................................................................................................................... viii
Hazardous Substances.......................................................................................................... viii
Operating...............................................................................................................................ix
Maintenance ..........................................................................................................................ix
Symbols used on the Centrifuge ......................................x
Symbols used in the Manual .........................................xi
Transport and Set Up .....................................I–1
1. Location ......................................................I–1
2. Transporting...................................................I–2
3. Setting Up ....................................................I–3
Setting Up........................................................................................................................... I–3
Level the Centrifuge............................................................................................................. I–4
4. Mains Connection ..............................................I–5
5. Storage.......................................................I–5
6. Shipping......................................................I–6
ThermoScientific
Centri-Touch User Interface............................... II–1
Status .........................................................II–2
Run Parameters .................................................II–3
Control and Configuration .........................................II–3
Lighthouse Mode ................................................II–4
Operation .............................................III–1
1. Switch on the Centrifuge .......................................III–1
2. Open the Centrifuge Door .......................................III–1

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Thermo Scientific Sorvall BIOS A Centrifuges
3. Install a Rotor ................................................III–2
3. 1. Before Installation.................................................................................................III–2
3. 2. Rotor Installation ..................................................................................................III–2
4. Maximum Loading.............................................III–5
Use of Tubes and Consumables...........................................................................................III–6
5. Close the Centrifuge Door.......................................III–6
6. Entering Parameters ...........................................III–6
6. 1. Acceleration / Deceleration Profiles .......................................................................III–6
6. 2. Preselect Speed / RCF-Value ................................................................................III–6
6. 3. Runtime Preselection............................................................................................III–7
6. 4. Preselect Temperature..........................................................................................III–7
6. 5. Pre-warm or Pre-cool the Centrifuge.....................................................................III–7
7. Programs ....................................................III–8
8. Centrifugation ................................................III–9
8. 1. Start ....................................................................................................................III–9
8. 2. Stop ....................................................................................................................III–9
8. 3. Using the Drain Box............................................................................................III–10
9. Remove a Rotor ............................................. III–11
10. Switch off the Centrifuge .................................... III–12
Maintenance and Care ...................................IV–1
1. Cleaning Intervals ............................................ IV–1
2. Basics...................................................... IV–1
2. 1. Rotor and Accessories Inspection......................................................................... IV–2
3. Cleaning .................................................... IV–3
3. 1. Touchscreen ....................................................................................................... IV–3
3. 2. Filter Mat............................................................................................................ IV–4
4. Disinfection ................................................. IV–5
5. Decontamination ............................................. IV–5
6. Autoclaving ................................................. IV–6
7. Maintenance ................................................ IV–6
7. 1. Preventive Maintenance....................................................................................... IV–6
7. 2. Service ............................................................................................................... IV–6
8. Shipping and Disposal ........................................ IV–7
Troubleshooting .........................................V-1
1. Mechanical Emergency Door Release ............................. V-1

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Thermo Scientific Sorvall BIOS A Centrifuges
2. Ice Formation................................................. V-2
3. Troubleshooting by Guide ....................................... V-3
4. Information for the Customer Service ............................. V-4
Technical Specifications ................................. A–1
1. Directives, Standards and Guidelines .............................A–2
2. Refrigerants ..................................................A–2
3. Mains Supply .................................................A–3
4. Accessories ..................................................A–3
Rotors ................................................ B–1
Items Supplied for Rotor ..........................................B–1
Fiberlite F6-10x1000 LEX Rotor.....................................B–2
Chemical Compatibility Chart ............................. C–1

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Thermo Scientific Sorvall BIOS A Centrifuges
Table of Figures
Figure I–1: Centrifuge clearance, example with barcode scanner.......................................... I–2
Figure I–2: Position of the bubble in the water level ............................................................. I–4
Figure I–3: Mains connection on the backside of the centrifuge............................................ I–5
Figure III–1: Overview centrifuge and rotor..........................................................................III–2
Figure III–2: Rotor installation.............................................................................................III–3
Figure III–3: Tighten rotor on the drive shaft, e.g. HAEMAFlex 12 .........................................III–3
Figure III–4: Correct Loading ..............................................................................................III–4
Figure III–5: Incorrect Loading............................................................................................III–4
Figure III–6: Put the rotor lid on the rotor body....................................................................III–5
Figure III–7: Select program...............................................................................................III–8
Figure III–8: Drain box (75007730)...................................................................................III–10
Figure III–9: Installing the drain box (75007730)...............................................................III–10
Figure III–10: Remove the plug.........................................................................................III–10
Figure III–11: Remove rotor lid from rotor body .................................................................III–11
Figure III–12: Disengage the rotor locking pin from drive shaft...........................................III–12
Figure III–13: Remove the rotor ........................................................................................III–12
Figure V–1: Marking on rotor lid..........................................................................................V-1
Figure V–2: Position of the mechanical emergency door release...........................................V-2
Figure V–3: Example for an error message ..........................................................................V-3
Table of Tables
Table Preface–1: Items Supplied ............................................................................................vi
Table Preface–2: Signal Words and Colors............................................................................. vii
Table Preface–3: Symbols used on the centrifuge ....................................................................x
Table Preface–4: Symbols used in the manual ........................................................................xi
Table I–1: Overview of necessary tools for setting up ........................................................... I–3
Table V–1: Error messages .................................................................................................V-3
Table A–1: Technical Data Thermo Scientific Sorvall BIOS A and Sorvall BIOS A Heavy Duty ..A–1
Table A–2: Directives, Standards and Guidelines Sorvall BIOS A and Sorvall BIOS A
Heavy Duty.........................................................................................................................A–2
Table A–3: Refrigerants used in the Sorvall BIOS A and Sorvall BIOS A Heavy Duty...............A–2
Table A–4: Mains Supply....................................................................................................A–3
Table A–5: Accessories ......................................................................................................A–3
Table B–1: Items supplied for rotor.....................................................................................B–1
Table B–2: F6-10x1000 mL with 1000 mL Bottles,PPCO....................................................B–2
Table C–1: Chemical Compatibility....................................................................................C–11

Thermo Scientific Sorvall BIOS A Centrifuges
Preface
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Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions.
Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers
Warranty.
Items Supplied
The centrifuges are supplied without a rotor. The items supplied with a rotor are listed in “Rotors” on page B–1.
Article No. Item Quantity
Centrifuge 1
75007698 Thermo Scientific Sorvall BIOS A,
200, 208, 220, 230, 240 V ±10%, 60 Hz, Single phase
Japan only: 200 V ±10%, 50 Hz
75007699 Thermo Scientific Sorvall BIOS A,
380, 400, 415 V ±10%, 50/60 Hz, 3-phase
75007700 Thermo Scientific Sorvall BIOS A Heavy Duty,
200, 208, 220, 230, 240 V ±10%, 60 Hz, Single phase
Japan only: 200 V ±10%, 50 Hz
Power Supply Cable 1
For Single phase units with 200, 208, 220, 230, 240 V
NEMA 6-50P 50A, 250 V
(Permanently connected with the device at the factory)
For 3-phase units with 380, 400, 415 V
20190369 IEC60309 32A-6h 5 pin red (3P+N+PE),
220/380 V; 230/400 V; 240/415 V
20280119 Bubble Level 1
Instruction Manual 1
Table Preface–1: Items Supplied
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.

Thermo Scientific Sorvall BIOS A Centrifuges
Preface
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Intended Use for Sorvall BIOS A and Sorvall BIOS A
Heavy Duty
This centrifuge is designed to separate sample mixtures of different densities like chemicals, environmental samples and other
non-human body samples.
Associated Rotors and Accessories
The Thermo Scientific™Sorvall™BIOS A and Thermo Scientific Sorvall BIOS A Heavy Duty centrifuge can be operated with
the Thermo Scientific™Fiberlite™F6-10x1000 LEX rotor. For more details on accessories refer to “Rotors” on page B–1 and
“Accessories” on page A–3.
Precautions
Signal Word and Color Degree of Hazard
WARNING Indicates a hazardous situation that, if not avoided, could result in death or serious injury.
CAUTION Indicates a hazardous situation that, if not avoided, could result in minor or moderate injury.
NOTICE Indicates information considered important, but not hazard-related.
Table Preface–2: Signal Words and Colors
WARNING NOT FOLLOWING THESE PRECAUTIONS CAN LEAD TO HAZARDOUS SITUATIONS THAT, IF NOT AVOIDED, COULD
RESULT IN DEATH OR SERIOUS INJURY.
Observe the safety instructions. Not following these instructions can cause damage.
The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with
fatal consequences.
The centrifuge must be operated by trained personnel only.
CAUTION Due to air friction the temperature of rotor may raise significantly while the centrifuge is spinning. Refrigerated
units have limitations in cooling capabilities. Displayed and set temperature can deviate from sample temperature.
Sample temperature might exceed critical temperature of your application.
WARNING The magnets built into the rotors can have a negative effect on active implants, such as cardiac pacemakers. The
magnets are mounted on the bottom of the rotor.
Always keep a distance of 20 cm between the rotor and the active implant, as the product generates permanent
magnetic fields. The magnetic field strength at a distance of 20 cm is less than 0.1 mT, so there should be no
interference.
Set Up Conditions
Plug the centrifuge only into sockets which have been properly grounded.
As safety zone maintain a clear radius of at least 30 cm around the centrifuge.
Do not place any dangerous substances within this safety zone.
Set up in a well-ventilated environment, on a horizontally leveled and rigid surface with adequate load-bearing capacity.

Thermo Scientific Sorvall BIOS A Centrifuges
Preface
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The mains plug must be freely accessible at all times.
To shut down the centrifuge: Press the STOP key. Turn off the centrifuge at the main switch. Pull out the power supply
plug. In an emergency disconnect the power supply.
Preparation
It is the obligation of the operator to make sure, that the proper protective clothing is used. Mind the “Laboratory Biosafety
Manual” of the World Health Organization (WHO) and the regulations in your country.
Do not make any changes to the mechanical components of the centrifuge or rotor. Do not replace mechanical
components.
Do not touch the electronic components of the centrifuge. Do not make any changes to the electronic components. Do not
replace electronic components.
Use only a properly installed rotor. Follow the instructions in section “Install a Rotor” on page III–2.
Do not use rotors or accessories which show any signs of removed protective coating, corrosion or cracks. Contact
customer service for further advice or inspections.
Use only with rotors which have been loaded properly.
Never overload the rotor.
Always balance the samples.
Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific.
Make sure the rotor is locked properly into place before operating the centrifuge.
Implement measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it
is running.
Hazardous Substances
Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts and the centrifuge
chamber have to be cleaned thoroughly.
Do not centrifuge explosive or flammable materials or substances.
The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone
environment.
Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions.
If centrifuging any hazardous materials mind the “Laboratory Biosafety Manual” of the World Health Organization (WHO) and
any local regulations. When centrifuging microbiological samples from the Risk Group II (according to the “Laboratory Biosafety
Manual” of the World Health Organization (WHO)), aerosol-tight biological seals have to be used. Look on the internet page of the
World Health Organization (www.who.int) for the “Laboratory Biosafety Manual”. For materials in a higher risk group, extra safety
measures have to be taken.
If toxins or pathogenic substances have contaminated the centrifuge or its parts, appropriate disinfection measures have
to be taken (“Disinfection” on page IV–5).
Extreme care should be taken with highly corrosive substances which can cause damage and impair the mechanical
stability of the rotor. These should only be centrifuged in fully sealed tubes.
If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately.

Thermo Scientific Sorvall BIOS A Centrifuges
Preface
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Operating
Never use the centrifuge if parts of its casing is damaged or missing.
Do not move the centrifuge while it is running.
Do not lean on the centrifuge.
Do not place anything on top of the centrifuge during a run.
Never open the centrifuge door until the rotor has come to a complete stop and this has been confirmed on the
touchscreen.
The emergency door release may be used in emergencies only to recover the samples from the centrifuge, e.g. during a
power failure (“Mechanical Emergency Door Release” on page V-1).
Do not open the centrifuge, while it is running.
In any case of severe mechanical failure, such as rotor or bottle crash, the centrifuge is not aerosol-tight.
In case of rotor failure the centrifuge can be damaged. Leave the room. Inform customer service.
Maintenance
The centrifuge housing is not to be opened by the operator.

Thermo Scientific Sorvall BIOS A Centrifuges
Preface
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Symbols used on the Centrifuge
This symbol refers to general hazards. Observe the information contained in the instruction
manual to keep yourself and your environment safe.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol refers to hazards from sharp items.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol refers to hazards from closing mechanical parts.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol refers to information on hazards, described within the manual.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol demands to disconnect mains before transporting or servicing the centrifuge.
This symbol shows the direction of rotation.
Table Preface–3: Symbols used on the centrifuge