Beckman Coulter JS-5.2 User manual

J6-TB-006AP
September 2016
Instructions For Use
JS-5.2 and JS-4.0
Swinging-Bucket Rotors
JS-5.2 Rotor for Use in
Beckman Coulter J6 Series
Centrifuges
JS-4.0 Rotor for Use in
Beckman Coulter Avanti JXN-26,
J-26XP Series and J6 Series Centrifuges
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

JS-5.2 and JS-4.0 Swinging-Bucket Rotors
J6-TB-006AP (September 2016)
Copyright © 2016 Beckman Coulter, Inc.
Avanti, Beckman Coulter, and the stylized logo are
trademarks of Beckman Coulter, Inc. and are registered in
the USPTO.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
All rights reserved. No part of this document may be
reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.
Find us on the World Wide Web at:
www.beckmancoulter.com

J6-TB-006AP iii
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to use this equipment. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to
how to proceed in any situation, contact your Beckman Coulter Representative.
This safety notice summarizes information basic to the safe use of the rotors described in this
manual. The international symbol displayed to the left is a reminder to the user that all safety
instructions should be read and understood before operation or maintenance of this equipment is
attempted. When you see the symbol on other pages of this publication, pay special attention to the
safety information presented. Observance of safety precautions will also help to avoid actions
that could damage or adversely affect the performance of the rotor. These rotors were developed,
manufactured, and tested for safety and reliability as part of a Beckman Coulter ultracentrifuge/
rotor system. Their safety or reliability cannot be assured if used in an ultracentrifuge not of
Beckman Coulter’s manufacture or in a Beckman Coulter ultracentrifuge that has been modified
without Beckman Coulter’s approval.
Alerts for Danger, Warning, Caution, and Note
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
NOTE
NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety Information for the JS-5.2 and JS-4.0 Rotors
Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that such fluids are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) viruses, HIV (I–V), atypical mycobacteria, and certain systemic fungi—further emphasize the
need for aerosol protection. Handle other infectious samples according to good laboratory
!

J6-TB-006AP
iv
Safety Notice
Safety Information for the JS-5.2 and JS-4.0 Rotors
procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe
proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive
materials in these rotors without taking appropriate safety precautions. Biosafe containment
should be used when Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level
of protection.
Dispose of all waste solutions according to appropriate environmental health and safety guidelines.
The rotors and accessories are not designed for use with materials capable of developing flammable
or explosive vapors. Do not centrifuge such materials in nor handle or store them near the
centrifuge
Components or accessories designed for other rotors may cause rotor mishap if used in these rotors.
Use only components and accessories that have been designed for use in these rotors. The safety
of rotor components and accessories made by other manufacturers cannot be ascertained by
Beckman Coulter. Use of other manufacturers’ components or accessories in the rotors may void the rotor
warranty and should be prohibited by your laboratory safety officer. If tubes, microplates, or other
labware made by manufacturers other than Beckman Coulter are used, reduce rotor speed to
prevent breakage. The strength of glass and plastic tubes can vary between lots, and will depend on
handling and usage; we highly recommend that you pretest labware in the rotor using water
samples to determine optimal operating conditions. Scratches (even microscopic ones) significantly
weaken glass tubes.
All four positions on the rotor yoke must contain either a bucket or a carrier (loaded or unloaded)
during a run. Never run the rotor without all of the positions being filled.
If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor.
Apply appropriate decontamination procedures to the centrifuge and accessories.
Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section on Run
Speeds, and derate the run speed as appropriate.
Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins
in scratches and may open fissures in the rotor with continued use.

v
Contents
Safety Notice,iii
Alerts for Danger, Warning, Caution, and Note,iii
Safety Information for the JS-5.2 and JS-4.0 Rotors,iii
JS-5.2 and JS-4.0
Swinging-Bucket Rotors,1
Specifications for the JS-5.2 Rotor,1
Specifications for the JS-4.0 Rotor,2
Description,2
Preparation and Use,3
Prerun Safety Checks,3
Rotor Preparation,3
Buckets and Accessories,4
Bottle Sleeve,4
Multi-Disc Adapters,4
Blood Bag Cups,5
Aeroseal Covers for Buckets,5
Tubes and Bottles,5
Temperature Limits,9
Thickwall Tubes,9
Polycarbonate and Polypropylene Bottles,9
Stainless Steel Tubes,9
Microfuge Tubes,10
Microplate Carriers,10
Operation,11
Installing the Rotor,11
Installing the Buckets or Microplate Carriers,14
Loading the Buckets and Carriers,15
Symmetric and Balanced Loading,15
Loading Buckets,18
Assembling Multi-Disc Adapters,18
Loading Adapters,20
Loading Blood Bag Cups,20
Loading Microplate Carriers,21
Run Speeds,21

vi
Contents
Run Temperature,22
Avanti J Series Centrifuges,22
Microprocessor-Controlled J6 Series Centrifuges,22
Care and Maintenance,24
Inspection,24
Maintenance,24
Cleaning,25
Rotor Components,25
Buckets,26
Multi-Disc Adapters,26
Decontamination,27
Sterilization and Disinfection,27
Returning a Rotor,28
Supply List,28
Replacement Rotor Parts,29
Other,29
Beckman Coulter, Inc.
J-Series Swinging-Bucket Rotor Warranty
Replacement,1
Conditions,1
Disclaimer,2
Related Documents

vii
Illustrations
Illustrations
1 Rotor Drive Pin Location and Orientation,12
2 Centrifuge Drive Spindle Hub Configuration,12
3 Using the Tie-Down Bolt and Tie-Down Tool to Tighten the
Rotor onto the Drive Hub,13
4 Using the Tie-Down Screw and Torque Bar to Tighten the Rotor
onto the Drive Hub (Older Rotors),14
5 Examples of Correctly and Incorrectly Loaded Buckets and
Carriers,17
6 Examples of the Effects on the Horizontal Swing of Buckets
Caused by Proper and Improper Weight Distribution,18
7 Assembling a Multi-Disc Adapter,18
8 Brackets (Vertical Supports) for the JS-5.2-Style Multi-Disc
Adapters and the GH-3.8-Style Modular Disk Adapters,19

viii
Tables
Tables
1 Available Tubes and Bottles for the JS-5.2 and JS-4.0 Swinging-
Bucket Rotors,5
2 Multi-Disc Adapters Used with Tubes and Bottles,8
3BloodBagCups,9
4 Labware for Use with Microplate Carriers,10
5 Relative Centrifugal Fields,22
6 Temperature Compensation Settings for J6 Series Centrifuge,23

J6-TB-006AP 1-1
JS-5.2 and JS-4.0
Swinging-Bucket Rotors
Specifications for the JS-5.2 Rotor
NOTE The JS-5.2 rotor is used in Beckman Coulter J6 series centrifuges only. It cannot be used in
Avanti J series centrifuges.
1. Axis of Rotation
U.S. Patent No.. 4,009,824
U.S. Pat. No. 4,010,890
Canadian Pat. No.
1,063,989
French Pat. No. 77 00732
Japanese Util. Mod. No.
U.M. 1,462,551
Maximum speed 5200 RPM
Critical speed range
a
600 to 800 RPM
Maximum solution density 1.2 g/mL
Maximum allowable imbalance of opposing loads 10 grams
Relative Centrifugal Field
b
at maximum speed
At r
max
(226 mm) 6840 g
At r
av
(156 mm) 4720 g
At r
min
(86 mm) 2600 g
Number of buckets 4
Nominal capacity per bucket 1 liter bottle or 1 quad pack blood bag
Nominal capacity of rotor4 liters, 4 blood bags, 12 microplates
148 RIA tubes
Approximate acceleration time to maximum speed
(rotor fully loaded)2
1
/2 min
Approximate deceleration time from maximum speed
(rotor fully loaded) 3 min
Weight of fully loaded rotor 21 kg (46 lb)
Rotor and bucket material anodized aluminum
Conditions requiring speed reductions see Run Speeds
Rotor entry code for microporcessor-controlled instruments 5.2
a. The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass. Passing through the critical
speed range is characterized by some vibration.
b. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rw
2
) to the standard acceleration of
gravity (g) according to the following formula: RCF = r/g — where ris the radius in millimeters, is the angular velocity in radians per second
(2 RPM /60), and gis the standard acceleration of gravity (9807 mm/s
2
). After substitution: RCF = 1.12r (RPM/1000)
2
rav
rmax
rmin
1

J6-TB-006AP
1-2
JS-5.2 and JS-4.0 Swinging-Bucket Rotors
Specifications for the JS-4.0 Rotor
Specifications for the JS-4.0 Rotor
NOTE The JS-4.0 rotor can be used in Beckman Coulter Avanti JXN-26, J-26XP series, discontinued Avanti J-20
series, and J6 series centrifuges.
Description
These Beckman Coulter rotors have been manufactured in an ISO 9001 or 13485 facility for use with the
specified Beckman Coulter ultracentrifuges.
The JS-5.2 and JS-4.0 swinging-bucket rotors are rated for maximum speeds of 5200 and 4000 RPM,
respectively. These rotors share a common four-place rotor yoke; the JS-5.2 has a windshield around
the yoke and buckets, and the JS-4.0 has no windshield. Each rotor holds four buckets or four
microplate carriers that hook over stainless steel pins set in the yoke and swing out to horizontal
1. Axis of Rotation
U.S. Pat. No. 4,009,824
Maximum speed 4000 RPM
Critical speed range
a
600 to 800 RPM
Maximum solution density 1.2 g/mL
Maximum allowable imbalance of opposite loads 10 grams
Relative Centrifugal Field
b
at maximum speed
At r
max
(226 mm) 4050 g
At r
av
(156 mm) 2800 g
At r
min
(86 mm) 1540 g
Number of buckets 4
Nominal capacity per bucket 1 liter bottle or 1 quad-pack blood bag
Nominal capacity of rotor 4 liters, 4 blood bags,
11112 microplates, 148 RIA tubes
Approximate acceleration time to maximum speed
(rotor fully loaded) 2 min
Approximate deceleraton time from maximum speed
(rotor fully loaded) 1
1
/2 min
Weight of fully loaded rotor 17 kg (37 lb)
Rotor and bucket material anodized aluminum
Conditions requiring speed reductions see Run Speeds
Rotor entry code for microprocessor-controlled instruments 4.0
a. The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass. Passing through the critical
speed range is characterized by some vibration.
b. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rw
2
) to the standard acceleration of
gravity (g) according to the following formula: RCF = r/g — where ris the radius in millimeters, is the angular velocity in radians per
second (2 RPM /60), and gis the standard acceleration of gravity (9807 mm/s
2
). After substitution: RCF = 1.12r (RPM/1000)
2
rav
rmax
rmin
1

J6-TB-006AP 1-3
JS-5.2 and JS-4.0 Swinging-Bucket Rotors
Preparation and Use
position during centrifugation. The buckets hold a wide variety of sample containers, including
tubes, bottles, and blood bag cups. The microplate carriers are used to perform serial dilution of
small liquid volumes.
Rotor assemblies, buckets, and lids are made of anodized aluminum.
See the Warranty at the back of this manual for warranty information.
Preparation and Use
Specific information about the JS-5.2 and JS-4.0 rotors is given here. Use the J Series Rotors and Tubes
Manual (publication JR-IM) along with this rotor manual for complete rotor and accessory information.
NOTE
Although rotor components and accessories made by other manufacturers may fit in the JS-5.2 and
JS-4.0 rotors, their safety in these rotors cannot be ascertained by Beckman Coulter. Use of other
manufacturers’ components or accessories in these rotors may void the rotor warranty and should be
prohibited by your laboratory safety officer. Only the components listed in this publication should be used
in these rotors.
Prerun Safety Checks
Read the Safety Notice section at the front of this manual before using the rotor.
1 Make sure that the rotor and lid, if applicable, are clean and show no signs of corrosion or
cracking.
2 Verify that the tubes, bottles, and other labware being used are listed in Table 1 through Table 4.
3 Check the chemical compatibilities of all materials used.
• Refer to Chemical Resistances (publication IN-175), included in the Rotors and Tubes CD.
Rotor Preparation
For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration.
1 Before installing the rotor, lightly coat the centrifuge drive hub with
Spinkote lubricant (306812).
2 Load the filled containers symmetrically into the rotor.

J6-TB-006AP
1-4
JS-5.2 and JS-4.0 Swinging-Bucket Rotors
Preparation and Use
a. If you are running two containers, place containers opposite each other on the yoke and
place empty buckets in the other positions.
• Opposing containers must be filled to the same level with liquid of the same density.
Buckets and Accessories
The round buckets used in the JS-5.2 and JS-4.0 rotors can hold bottle adapters, Multi-Disc adapters
to accommodate tubes of various sizes, and blood bag cups.
Bottle Sleeve
The one-liter bottles used in the JS-5.2 and JS-4.0 rotors are supported in a polypropylene sleeve
(356096) that fits inside the buckets. Bottles available for use are listed in Table 1.
Multi-Disc Adapters
The Multi-Disc adapters are made up of polypropylene discs, which are stacked and snapped
together to accommodate a particular size bottle or tube. The number of adapters required depends
on the length of the tube or bottle. The discs are color-coded for identification. See Table 2 for the
appropriate adapter discs for use with different size tubes and bottles
1. Bottle
2. Sleeve
1. Bails
1
2
1

J6-TB-006AP 1-5
JS-5.2 and JS-4.0 Swinging-Bucket Rotors
Preparation and Use
Blood Bag Cups
Polypropylene cups provide support for blood bags in the rotor buckets. A single or double pack
blood bag cup, and a triple or quad pack cup, are available and are listed in Table 3.
Aeroseal Covers for Buckets
The Aeroseal covers
*
(343686) are designed to minimize leakage of aerosol particles from buckets
during centrifugation. Aeroseal covers can be used with Multi-Disc adapters (with modified bracket
343369) but not with blood bag cups. For more information, refer to publication J6-TB-017, which is
shipped with the covers.
Tubes and Bottles
The JS-5.2 and JS-4.0 rotors use the tubes and bottles listed in Table 1. Be sure to use only those items
listed, and to observe the maximum speed limits and fill volumes shown. Refer to Appendix A in
Rotors and Tubes for chemical compatibilities of tube, bottle, and accessory materials.
* U.S. Pat. No. 4,342,419.
1. Plug
2. O-ring
1
2
Tab le 1 Available Tubes and Bottles for the JS-5.2 and JS-4.0 Swinging-Bucket Rotorsa
Tube Max Fill
Volumeb
(mL)
Required Accessory Max
Speedc
Dimensions/
and Volume/
Description Part
Number
Description Part
Number
97 167 mm
1000 mL
polycarbonate bottle
w/screw cap
355675 1000 sleeve 356096 5200 RPM
97 167 mm
1000 mL
polypropylene bottle
w/screw cap
355676 1000 sleeve 356096 520p RPM

J6-TB-006AP
1-6
JS-5.2 and JS-4.0 Swinging-Bucket Rotors
Preparation and Use
96 130 mm
750 mL
polycarbonate bottle
w/screw cap
358299 750 sleeve 356096 5200 RPM
96 139 mm
750 mL
polypropylene bottle
w/screw cap
356855 750 sleeve 356096 5200 RPM
69 160 mm
500 mL
polycarbonate bottle
w/screw cap
355664 500 adapter 339109 5200 RPM
69 160 mm
500 mL
polycarbonate wide-mouth
bottle w/cap assy
355605 500 adapter 339109 5200 RPM
69 160 mm
500 mL
polypropylene wide-mouth
bottle w/cap assy
355607 500 adapter 339109 5200 RPM
69 160 mm
500 mL
polycarbonate bottle,
no cap
355649 500 adapter 339109 5200 RPM
69 159 mm
500 mL
polypropylene bottle
w/screw cap
355665 500 adapter 339109 5200 RPM
69 159 mm
500 mL
polypropylene bottle,
no cap
355650 500 adapter 339109 5200 RPM
62 136 mm
250 mL
polycarbonate round-
bottom bottle w/screw cap
355673 250 adapter 339108 5200 RPM
62 122 mm
250 mL
polycarbonate wide-mouth
bottle
358275 250 adapter 339108 5200 RPM
62 122 mm
250 mL
polypropylene wide-mouth
bottle
358326 250 adapter 339108 5200 RPM
62 120 mm
250 mL
polypropylene wide mouth
bottle w/cap assy
356011 250 none — 5200 RPM
62 120 mm
250 mL
polycarbonate wide mouth
bottle w/cap assy
356013 250 none — 5200 RPM
60 120 mm
230 mL
conical polycarbonate
bottle w/screw cap
356987 230 adapter 356983/
339108
5200 RPM
60 120 mm
230 mL
conical polypropylene bottle
w/screw cap
356989 230 adapter 356983 5200 RPM
38 102 mm
70 mL
polycarbonate bottle
w/cap assy
355620 70 adapter 339104 5200 RPM
38 102 mm
70 mL
polycarbonate bottle,
no cap
355655 70 adapter 339104 5200 RPM
Tab le 1 Available Tubes and Bottles for the JS-5.2 and JS-4.0 Swinging-Bucket Rotorsa(Continued)
Tube Max Fill
Volumeb
(mL)
Required Accessory Max
Speedc
Dimensions/
and Volume/
Description Part
Number
Description Part
Number

J6-TB-006AP 1-7
JS-5.2 and JS-4.0 Swinging-Bucket Rotors
Preparation and Use
29 104 mm
50 mL
polypropylene bottle assy
w/snap-on cap
361694 50 adapter 356997 5200 RPM
29 104 mm
50 mL
polycarbonate open-top
tube
363647 50 adapter 356997 5200 RPM
29 104 mm
50 mL
polycarbonate bottle assy,
liquid-tight cap assy
357000 45 none — 5200 RPM
29 104 mm
50 mL
polypropylene bottle assy,
liquid-tight cap assy
357001 45 none — 5200 RPM
29 104 mm
50 mL
polycarbonate bottle
w/screw cap
357002 40 none — 5200 RPM
29 104 mm
50 mL
polypropylene bottle
w/screw cap
357003 40 none — 5200 RPM
29 104 mm
50 mL
polycarbonate tube
w/snap-on cap
363664 36.5 adapter 356997 5200 RPM
29 104 mm
50 mL
polypropylene thickwall
tube, snap-on cap
35700 36.5 white 29-mm cap
red 29-mm cap
green 29-mm cap
yellow 29-mm cap
blue 29-mm cap
356264d
357359d
357360d
357361d
357362d
5200 RPM
29 104 mm
50 mL
polypropylene thickwall
tube, no cap
357007 34 none — 5200 RPM
18 98 mm
15 mL
polyethylene tube, open top 342081d 15 adapter 339102 5200 RPM
18 98 mm
15 mL
polycarbonate tube,
open top
342080e15 adapter 339102 5200 RPM
18 100 mm
15 mL
polypropylene tube,
open top
342082d 15 adapter 339102 5200 RPM
17 119 mm
15 mL
conical polypropylene
open-top tube
355663 15 adapter 356995 5200 RPM
16 80 mm
10 mL
polycarbonate bottle w/cap 355672 10 adapter 341977 5200 RPM
16 76 mm
10 mL
polycarbonate tube,
open top
355630 10 adapter 341977 5200 RPM
Tab le 1 Available Tubes and Bottles for the JS-5.2 and JS-4.0 Swinging-Bucket Rotorsa(Continued)
Tube Max Fill
Volumeb
(mL)
Required Accessory Max
Speedc
Dimensions/
and Volume/
Description Part
Number
Description Part
Number

J6-TB-006AP
1-8
JS-5.2 and JS-4.0 Swinging-Bucket Rotors
Preparation and Use
16 76 mm
10 mL
polypropylene tube, open
top
355640 10 adapter 341977 5200 RPM
14 55 mm
4 mL
polypropylene
Bio-vial
566353 4 adapter 339101 5200 RPM
11 39 mm
1.5 mL
polypropylene tube
w/snap-on cap
357448 1.5 adapters 339100/
354511
5200 RPM
11 39 mm
1.5 mL
polypropylene tube
w/snap-on cap
343169 1.5 adapters 339100/
354511
5200 RPM
11 39 mm
1.5 mL
polyethylene tube
w/snap-on cap
340186 1.5 adapters 339100/
354511
5200 RPM
a. Use only the items listed here and observe fill volumes and maximum run speeds.
b. Above 20°C fill polypropylene tubes at least half full.
c. Maximum speeds listed are for the JS-5.2 rotor, and are guidelines only. These speeds have been achieved in reliability tests at Beckman Coulter,
but, because of manufacturing variances, no guarantee of performance or fit is expressed or implied. The maximum speed of all tubes and bottles
in the JS-4.0 rotor is 4000 RPM.
d. Package of 25.
e. To order caps for 15-mL tubes 342080, 342081, and 342082, use part number 343656 for a package of 50. Caps 343656 are made of Hytrel
thermoplastic polyester elastomer. Hytrel is a registered trademark of E.I. Du Pont de Nemours & Co. Note that Hytrel does not provide
the same chemical resistance as the tube materials. Before using the caps, check with the manufacturer to verify Hytrel’s ability to withstand
exposure to the chemicals you will be using.
Tab le 1 Available Tubes and Bottles for the JS-5.2 and JS-4.0 Swinging-Bucket Rotorsa(Continued)
Tube Max Fill
Volumeb
(mL)
Required Accessory Max
Speedc
Dimensions/
and Volume/
Description Part
Number
Description Part
Number
Tab le 2 Multi-Disc Adapters Used with Tubes and Bottles
Typical Tube/
Bottle Sizes
Used
Max Tube DimensionsaNumber of
Tubes per
Adapter
Number of
Tubes per
Rotor
Adapter
Part
Number
Color
Diameter Length
3 and 5 mL 12 mm 133 mm 37 148 339100 blue
10 mL 14 mm 133 mm 24 96 339101 orange
12 mL 16 mm 133 mm 19 76 341977 purple
20 mL 18 mm 133 mm 14 56 339102bgreen
50 mL 28 mm 136 mm 7 28 339103 yellow
50 mL (conical) 30 mm 136 mm 4 16 345386 lt. green
50 mL 35 mm 136 mm 4 16 341794 dark blue
100 mL 44 mm 165 mm 2 8 339104 brown
230 mL (conical) 62 mm 141 mm 1 4 339108 red
250 mL 62 mm 160 mm 1 4 339108 red

J6-TB-006AP 1-9
JS-5.2 and JS-4.0 Swinging-Bucket Rotors
Preparation and Use
Temperature Limits
• Plastic tubes have been centrifuge tested for use at temperatures between 2 and 25°C. For
centrifugation at other temperatures, pretest tubes under anticipated run conditions.
• If plastic containers are frozen before use, make sure that they are thawed to at least 2°C prior
to centrifugation.
Thickwall Tubes
Thickwall polypropylene, and polycarbonate tubes can be run partially filled with or without caps,
but all opposing tubes for a run must be filled to the same level with liquid of the same density. Do
not overfill capless tubes.
Polycarbonate and Polypropylene Bottles
Capped polycarbonate and polypropylene bottles may be run completely filled, or partially filled.
All opposing containers for a run must be filled to the same level.
Stainless Steel Tubes
Stainless steel tubes offer excellent resistance to organic solvents and heat, but should not be used
with most acids or bases. They offer only marginal resistance to most gradient-forming materials
500 mL 70 mm 160 mm 1 4 339109 yellow
Double-Stacking Adapterc
3 and 5 mL 12 mm 75 mm 19 76 339119 blue/white
a. Additional discs can be added.
b. This adapter also holds 15-mL conical tubes.
c. To run 148 RIA tubes, use four of the 37-hole adapters (339100) plus four of the 19-hole adapters (339119). Tube retaining
device 343108 is required; use of this device is described in publication IN-174, which ships with 343108.
Tab le 3 Blood Bag Cups
Description Blood Packs
per Cup
Number of
Cups per
Rotor
Part
Number
(set of 2)
Blood bag cups (yellow)
88 mm inside diameter
1 single or
double pack
4339127
Blood bag cups (red)
98 mm inside diameter
1 triple or
quad pack
4339129
Tab le 2 Multi-Disc Adapters Used with Tubes and Bottles
Typical Tube/
Bottle Sizes
Used
Max Tube DimensionsaNumber of
Tubes per
Adapter
Number of
Tubes per
Rotor
Adapter
Part
Number
Color
Diameter Length

J6-TB-006AP
1-10
JS-5.2 and JS-4.0 Swinging-Bucket Rotors
Preparation and Use
other than sucrose and glycerol. Stainless steel tubes are very strong and can be centrifuged when
filled to any level. Stainless steel tubes can be used indefinitely if they are undamaged and not
allowed to corrode. They may be autoclaved as long as they are thoroughly dried before storage.
Microfuge Tubes
Microfuge tubes, 1.5-mL tubes with attached caps, are made of clear or colored polypropylene. They
are used in microplate carriers. The number and arrangement of microfuge tubes in opposing
carriers should be balanced.
Microplate Carriers
Microplate carriers (358680, qty/2) are installed on the rotor pivot pins in place of the buckets
normally used with the rotor. The carriers are made of aluminum and are black-anodized for
corrosion resistance. Each microplate carrier can accommodate up to three 96-well multiwell plates
(when more than one plate is run, up to three plates are placed directly on top of one another).
Refer to Table 4 for a list of labware that can be used with the microplate carriers. Rotor speed must
not exceed 2600 RPM when microplate carriers are run. For complete microplate carrier
information, see publication J6-TB-009, included with the carriers.
Tab le 4 Labware for Use with Microplate Carriers
Description Volume Part
Number
Required Accessory Rack
Description Part
Number
Multiwell plate, 96-well,
nonsterile, without caps
300 L/well 609844 — — —
Microfuge tube,
polypropylene, clear
1.5 mL 357448 Rack insert,
white
373696 373661
holds 24 tubes)
Microfuge tube,
polypropylene, clear
1.5 mL 356090 Rack insert,
white
373696 373661
(holds 24 tubes)
Microfuge tube,
polypropylene, blue
1.5 mL 356091 Rack insert,
white
373696 373661
(holds 24 tubes)
Microfuge tube,
polypropylene, green
1.5 mL 356092 Rack insert,
white
373696 373661
(holds 24 tubes)
Caution:
DoNot Exceed
2500RPM

J6-TB-006AP 1-11
JS-5.2 and JS-4.0 Swinging-Bucket Rotors
Preparation and Use
Operation
For low-temperature runs, precool the rotor in the centrifuge or in a refrigerator before use—especially
before short runs—to ensure that the rotor reaches the set temperature. A suggested precooling cycle is a
minimum of 30 minutes at 2000 RPM at the required temperature.
1 If fluid containment is required, use capped tubes or bottles.
• It is strongly recommended that all containers carrying physiological fluids be capped to
prevent leakage.
2 If you are using a microprocessor-controlled J6 series centrifuge, enter rotor code 5.2 for the
JS-5.2, or code
4.0 for the JS-4.0 rotor.
3 Apply a thin film of Spinkote lubricant to the centrifuge drive spindle hub.
4 Refer to the centrifuge instruction manual for additional operating instructions.
Installing the Rotor
Two drive pins inside the rotor drive hole (see Figure 1) engage with teeth on the centrifuge drive
spindle hub to prevent the rotor from slipping during acceleration and deceleration. When the
rotor is properly installed, the pins sit in the drive hub grooves or next to the drive hub teeth
(see Figure 2).
Microfuge tube,
polypropylene, yellow
1.5 mL 356093 Rack insert,
white
373696 373661
(holds 24 tubes)
Microfuge tube,
polypropylene, orange
1.5 mL 356094 Rack insert,
white
373696 373661
(holds 24 tubes)
Tab le 4 Labware for Use with Microplate Carriers
Description Volume Part
Number
Required Accessory Rack
Description Part
Number

J6-TB-006AP
1-12
JS-5.2 and JS-4.0 Swinging-Bucket Rotors
Preparation and Use
Figure 1 Rotor Drive Pin Location and Orientation
Figure 2 Centrifuge Drive Spindle Hub Configuration
1 Hold the rotor with both hands and carefully lower the rotor yoke straight down onto the
centrifuge drive spindle hub.
a. Slowly rotate the yoke back and forth to make sure that it is properly seated.
1. Rotor Drive Hole
2. Centrifuge Drive Hub
3. Drive Pins
4. Drive Hub Teeth
1. Drive Hub Grooves (4)
2. Rotor Pins (2)
3. Drive Hub
4. Drive Spindle Assembly
5. Teeth (2)
6. Drive Hub
7. Drive Spindle Assembly
14
3
2
Older Model Centrifuges —
Be sure the pins in the rotor drive hole
are seated in the grooves
of the drive spindle hub as shown.
Newer Model Centrifuges —
Be sure the pins in the rotor drive hole
are not sitting on top of the teeth
on the drive spindle hub.
15
4
3
2
6
7