
SurfaceSamplingDevice
GENERALDESCRIPTION
TheLIGHTNINGMVPICONSurfaceSamplingDeviceisaself‐
containeddeviceforusewiththeLIGHTNINGMVPICON™or
LIGHTNING MVP®systemsforqualityandHACCP‐related
monitoringoftheenvironment.TheMVPICONand
LIGHTNINGMVPsystemsdetectfoodandmicrobiological
residuesbymeasuringAdenosinetriphosphate(ATP)using
luciferasebioluminescence.ATPisfoundinmostfood
residuesandallbacteria,yeastandmoldcells.
PartNo.64003‐100(100tests)
KITCOMPONENTS
Oneboxof100surfacesamplingdevices
IMPORTANTUSAGEGUIDELINES
Itiscriticaltoinsertthesamplingdevice
intotheMVPICONwiththetwoflat
sidesofthesamplingdevice’sbase
chamberfacingleftandright.Improper
samplingdeviceorientationmayresultin
damagetotheinstrument.
Donotinsertwetorcontaminated
samplingdevicesintotheinstrument.Wipecondensatefrom
exteriorofdevicebeforeusewithaclean,lint‐freecloth.
Avoidhandlingthedevicewithwetorcontaminatedhands.
Alwaysremovesamplingdevicesfrominstrumentafter
reading.
DonotstoretheMVPICONorMVPinstrumentonitssideor
back.Avoidstoringinhumidareaswherewaterleaksare
possible.
TESTPROCEDURE
A.CollectingSample
(a) Twisttoremovesamplingdevicefrombodytube.The
swabtipmustbemoist.Iffullysaturated,anyexcess
wettingagentshouldbeblottedoffbygentlypressing
theswabtipagainsttheinteriorofthebodytube.Be
carefulnottotouchtheswaborinsidethesampling
devicewithfingers.
(b) Thoroughlyswabastandard10x10cm(4x4in)areaof
interestforatypicalflatsurface.Forirregularlyshaped
surfaces,ensureswabbingtechniqueremainsconsistent
eachtimeyouswab.Reinserttheswabintothebody
tubeusingatwistingmotiontoensureitissealed.
Note:Afterswabbing,thesamplingdeviceisstableforone
hourpriortoactivation.
B.ActivatingDeviceandReadingResults
(a) Toactivatethesamplingdevice,holduprightandpush
theplungerdownuntilitisfullydepressed.Thereagents
willbedispensedthroughthesamplingdevicewashing
thesampleofftheswabtipandintothecollectiontube.
(b)InsertthesamplingdeviceintotheMVPICONorMVP
andreadtheresults.WheninsertingintotheMVPICON,
ensuretheflatsidesofthesamplingdevicebase
chamberfaceleftandrightintheinstrument.
Note:Resultsshouldbereadwithintwominutesof
activation.PleaserefertotheMVPICONUserGuideorMVP
SystemGuideforoperatinginstructions.
INTERPRETINGRESULTS
(a) WhenusingdefaultthresholdsintheMVPICONorMVP
systems,readingsof2.5Zonesorlessindicatethe
surfaceisconsideredclean.
(b) Readingsbetween2.6‐3.0Zonesindicateawarningthe
surfaceisnotadequatelyclean.
(c) Ifthereadingisgreaterthan3.0Zones,thesurfaceis
considereddirtyandshouldbere‐cleanedandre‐tested.
CONTROLS
A. PositiveControl
ItisrecommendedLIGHTNINGMVPICONATPPositive
Controls(PartNo.64001‐25)beruneveryotherdaytoverify
integrityofthereagentsystem.
(a) Apositivecontrolshouldyieldaresultfrom3.1to4.3
Zones.SeeATPPositiveControlDirectionsforUsefor
additionalinformation.
B. NegativeControl
AnegativecontrolisanunusedyetactivatedLIGHTNINGMVP
ICONSurfaceSamplingDevice.
(a) Negativecontrolresultsshouldreadbetween1.0to2.5
Zones.
(b) Readingsabove2.5mayindicatecontaminationofthe
MVPICONorMVPsamplingchamberorsamplingdevice.
SeeATPPositiveControlDirectionsforUseforadditional
information.ContactBioControlTechnicalSupportat
800.245.0113formoreinformation.
PRECAUTIONS
Theappearanceofayellowbandontheswabtipisnormal
andwillnotaffectperformance.
Ifaccidentalactivationpriortosamplingoccurs,donotuse
device.
FlatSide