Biocontrol Assurance GDS PPMX User manual

55240.R001102015
ASSURANCEGDS®PPMX
USERMANUAL
 
12822SE32ndStreet
Bellevue,WA,USA98005
Phone425.603.1123
Fax425.603.0070
[email protected]
www.biocontrolsys.com

ASSURANCEGDS®PPMXUSERMANUAL 1
TableofContents
I. Safety .................................................................................................................... 3
Voltage
SafetyInterlock
PipetteHeads
TipWasteContainer
Tray
Liquids
ReplacementParts
II. Introduction .......................................................................................................... 5
StandardEquipment
Documentation
Protocols
Disposables
TechnicalSpecifications
III. Setup/Operation................................................................................................... 8
Unpacking
RemovableTray
RearPanelConnections
FrontPanelConnections
PowerOnandStartUp
TouchscreenTabletOverview
AlignmentProtocol
LogIn
AddPipetteHead(s)
SetDate&Time
CreateUsers
RunaProtocol
Step‐by‐stepWizard
PauseorStopaRunningProtocol
ViewResults

ASSURANCEGDS®PPMXUSERMANUAL 2
RunReport
ImportProtocol
ExportProtocol
ChangeSerialNumberinTabletSoftware
IV. Troubleshooting.................................................................................................. 20
CommunicationLoss
Re‐startingaProtocolafterCommunicationLoss
HoodOpen
RefillTipRack
TechnicalServices
V. Maintenance....................................................................................................... 22
Cleaning
PipetteHeadCalibration
AlignmentCalibration
Warranty
InstrumentReturnProcedure
VI. AppendixA.......................................................................................................... 24
PartsList
VII. AppendixB.......................................................................................................... 26
BeforeYouCallUs
VIII. AppendixC.......................................................................................................... 27
MaintenanceLog
IX. AppendixD.......................................................................................................... 28
WarrantyTerms
X. AppendixE.......................................................................................................... 29
PipetteVerification
XI. AppendixF.......................................................................................................... 30
PackUp

ASSURANCEGDS®PPMXUSERMANUAL3
Safety
ReadthissectionbeforeinstallingandoperatingtheAssuranceGDS®PPMX.

Thisinstrumentisintendedtobeusedinalaboratoryenvironmentbytrainedtechnicalpersonnel.

Forsafeandproperuseofthisinstrument,itisrequiredthatbothoperatingandservicepersonnelfollowthe
instructions
 containedinthisguidewheninstalling,operating,cleaning,andmaintainingtheinstrument.

Thefollowingsafetyprecautionsmustbeobservedduringallphasesofoperation,service,andrepairofthe
instrument.Failure
 tocomplywiththeseprecautionsorwithspecificwarningselsewhereinthisuser’sguide
violatessafetystandardsofdesign,
 manufacture,andintendeduseoftheinstrument.BioControlSystems
assumesnoliabilityforthecustomer’sfailuretocomplywiththese
 requirements.
ThePPMXhasbeencertifiedtosafetystandardsrequiredinCanada,Europe,andtheUnitedStates.Refertothe

instrumentrearpanellabelandtheDeclarationofConformitydocumentforthecurrentstandardstowhichthe
instrumenthas
 beenfoundcompliant.
Thefollowingelectronicandhazardsymbolsmayappearontheinstrument:
Thefollowingsafetynoticesmayappearinthisdocument:
Voltage
Accesstotherearpanelisnecessarybecausetheinstrumentmustbedetachedfromallvoltagesourcesbefore
service,repair,
 orexchangeofparts.
Operatetheinstrumentusingtheapprovedpowersupplyprovidedandonlyatthevoltagespecifiedontherear
panellabelof
 theinstrument.

ASSURANCEGDS®PPMXUSERMANUAL4
SafetyInterlock
ThePPMXisconfiguredwithasafetyinterlock,whichisusedwhentherotatingcoverisinstalledonthe
instrument.
Thesafetyinterlockpreventstheinstrumentfromoperatingwhentherotatingcoverisopen.

PipetteHeads
Theinstrumentiscapableofgeneratingsignificantforcesthatcouldcausepotentialinjurytotheuser.Foran
instrumentwith
 therotatingcoverinstalled,thesafetyinterlockinthecoverwilldisabletheinstrumentmovement
ifthecoverontheinstrument
 isopen.Foraninstrumentinstalledinahood,theexternalsafetyinterlocksensor
mountedontheinstrumentwilldisablethe
 instrumentmovementifthehooddoorisopen.
TipWasteContainer
Thetipwastecontainershouldbeemptiedinaccordancewithnationalandlocalsafetyregulations.

Tray
Themovingtrayiscapableofcausinginjurybypinching.Foraninstrumentwiththerotatingcoverinstalled,the
safetyinterlock
 inthecoverwilldisabletheinstrumentmovementifthecoverontheinstrumentisopen.
Liquids
Observesafelaboratorypracticeswhenhandlingliquids.Ifworkingwithbiologicalsamplesorchemicalsubstances,
ensurethat
 thereisproperventilation,andwearpersonalprotectiveequipment(PPE),suchassafetyglasses,
gloves,andprotectiveclothing
 atalltimes.

RefertotheSafetyDataSheetsforsolventsbeforeuse.

ReplacementParts
Besuretouseonlyreplacementpartsspecifiedinthisusermanual.Donotrepairorchangepartswhicharenot
listedinthis
 usermanual.Ifitisnecessarytochangepartsnotlisted,pleasecontactBioControlSystems
technicalservices.

ASSURANCEGDS®PPMXUSERMANUAL5
Introduction
TheAssuranceGDS®PPMX®isanautomatedmagneticparticlesandliquidhandler.Itiscapableofdispensing
AssuranceGDSreagentswhichinclude,ConcentrationReagent,WashSolution,ResuspensionBufferandselect
media(BHI/DFB).ReagentdispenseprotocolshavebeencreatedforallAssuranceGDSassays.Inaddition,the
PPMXhasalsobeenprogrammedtoautomateAssuranceGDSPickPen®ImmunomagneticSeparation(IMS)
procedures.ThisusermanualisintendedtoassistPPMXuserswithsystemstart‐up,basicoperation,maintenance
andservice.
StandardEquipment
AfterthePPMXandaccessoriesareunpacked,thefollowingitemsshouldbepresent:

• PPMXInstrumentwithattachedAlignmentHead,Half8‐channelTipEjectorFootandrotatingcover
• ControlTabletwithTRILUTIONmicrosoftwareandAssuranceGDSprotocolspre‐loaded
• ControlTabletHolder
• RemovableTray96well(x2)
• MAX8x200PipetteHead
• TipReloadRack(x3)
• TipDisposalBin(x2)
• AssuranceGDSReagentHolder
• AssuranceGDSPickPenMagneticHead
• AssuranceGDSPickPenTipPuck
• AssuranceGDSPickPenStand
• PPMXGelCoolingBlock
• USBCable
• PowerSupply(110V/220V)
• PowerCords(USandEU)
• AllenWrench,5mm
• BlackPlugforOutletontheRearPanel

Forpartnumbers,refertoAppendixA,PartsList.



ASSURANCEGDS®PPMXUSERMANUAL6
Documentation
ThefollowingdocumentsareincludedwiththePPMX:

• UserManual
• QuickSetupGuide
• Installation/Operation/PerformanceQualification(IQ/OQ/PQ)Procedures
• DeclarationofConformity
• ValidationCertificateforTRILUTIONmicro
• GilsonQualityControlReportPipetteHead

Protocols
• AlignmentProtocol
• AssuranceGDSReagentDispenseprotocols(R‐)
• AssuranceGDSPickPenIMSprotocols(IMS‐)

Disposables
Theitemsbelowaresoldseparatelybutarerequiredforuse:
• 200µLBlisterTipPacks
• ReagentReservoirs
• AssuranceGDSPickPenTips
• AdhesiveFilmSheets&Strips
• AssuranceGDSSampleWells&Bases
• ResuspensionPlates

Forpartnumbers,refertoAppendixA,PartsList.

ASSURANCEGDS®PPMXUSERMANUAL7
TechnicalSpecifications
PleasebeawareofthefollowingbeforeoperatingthePPMX.

ChangesormodificationstothisdevicenotexpresslyapprovedbyBioControlSystems
couldvoidthewarranty.

Theinstrumentcomplieswithpart15oftheFCCRules.Operationissubjecttothefollowingtwoconditions:(1)
Thisinstrument
 maynotcauseharmfulinterference,and(2)thisinstrumentmustacceptanyinterference
received,includinginterferencethat
 maycauseundesiredoperation.

ShieldedcablesmustbeusedwiththeinstrumenttoensurecompliancewiththeFCCClassAlimits.

TechnicalSpecificationDefinition
CommunicationsUSB

ConnectionsThreeUSBhostportsandoneUSBdeviceport
Twoinputs(contactclosure,TTL),tworelayoutputs,andoneswitched+12VDC1Aoutput
NOTICE:Switchingvoltageshigherthan30Vorgreaterthan1Aofcurrentmaydamagethe
instrument

Dimensions54.4X65.5X53.1cm(21.4X25.8X20.9in)

EnvironmentalConditionsIndooruse
Altitude:upto2000m
Temperaturerange:5°C–40°C
Humidity:Maximumrelativehumidity80%fortemperaturesupto31°C,decreasinglinearity
to50%relativehumidityat40°C

PowerRequirementsExternalPowerSupply
VoltageInput
Frequency:50to60Hz
Voltage:100‐240VAC
VoltageOutput
Voltage:24VDC
CurrentRating:6.25A,150W

FrontPanel

TwoUSBhostportsandSTOPbutton
XYZMotion500mm/secinXdimension
1‐550mm/sec(350mm/secdefault)inYdimension(tray)
140m/secinZdimension

IMS(Immunomagnetic
Separation)head

PickPen®Magnetichead

IMS(Immunomagnetic
Separation)tips

PickPen®tipcovers

Liquidhandlinghead

MAX8x200

LiquidhandlingtipsDIAMONDfiltertips,DFL10

RemovabletrayCapacity

9‐position
SafetyandComplianceCertifiedtosafetystandardsspecifiedforCanada,EuropeandtheUnitedStates.Refertothe
instrumentrearpanellabelandtheDeclarationofConformitydocumentforthecurrent
standardstowhichtheinstrumenthasbeenfoundcompliant.


ASSURANCEGDS®PPMXUSERMANUAL8
Setup/Operation
TheAssuranceGDS®PPMX®anditscomponentsshouldbesetupintheorderdescribedinthissection.Gilson
TRILUTION®micro
 providessoftwarecontrolofthePPMXduringsetupandoperationasdescribedinthissection.
Formoreinformation
 aboutTRILUTION®micro,viewthehelpinformationinthesoftware,accessiblefromthe
button.

Unpacking
ThePPMXisdeliveredwithmostmajorcomponentsalreadyassembled.Keeptheoriginalpackagingincasethe
PPMX
 mustbereturnedtoBioControlSystemsforrepair.

Itisrecommendedthattwo
peopleliftthePPMXout
 ofthe
box,asitweighsapproximately55
lbs.(24.9kg).
1 Opentopofprintedcardboardbox.
2 Removetheopen‐endedcardboardbox.
3 Removetheboxofaccessoriesandopenit.
4 Lifttheprinted,outercardboardboxuptoremove.
5 GripthePPMXattherecessesnearthebase.Thereis
onerecessin
 thefront,oneintheback,andoneon
eachside.Usetheserecesses
 whenliftingthePPMX
outofthefoam‐lined,cardboardtray.

DonotattempttoliftthePPMXfromthecoveror
fromtheX‐arm(thehorizontalarm).Alwayslifttheinstrumentfromitsbase.
6 PlacethePPMXonastable,levelsurfacesuchasalabbench,labcart.RefertoSection1:Safety.
7 RemovethebagcoveringthePPMX.Youmayneedtoliftthefrontandthebackslightly.
8 Removethefilmprotectingtherotatingcover.
9 Removetheshippingbracefromtherearof
theinstrument.
 Loosenthethumbscrews
securingtheshippingbracetothe
 PPMX.
Afterremovingtheshippingbrace,replace
andtightenthe
 thumbscrews.
10 Removetheshippingbracketthatattachesthearmtotheinsideoftheunit
usingtheallenwrenchprovided.

11 Removethewhitefoamblockpreventingthetrayfrom
movingandsecuring
 thealignmentcablecord.

Keeptheshippingbrace,shippingbracket,allenwrench
andfoamblockwithpackaging.


ASSURANCEGDS®PPMXUSERMANUAL9
RemovableTray
Aremovabletrayforplacementoflabwareandtipracksisrequired.Whileholdingthehandlesonthesidesof
thetray,lowerthetrayonto
 themetalcarrier.Itiskeyedandwillonlymountoneway.Observethat  thetrayis
levelandfullyseatedonthemetalcarrier.

RearPanelConnections
Refertothediagramsonthenextpagewhenmakingthe
connectionsdescribedinthissection.
Theinput/outputcontactsontherearpanelareusedfor
communicationbetweenthealignmentheadandTRILUTION
micro
 andforcommunicationbetweentheexternalsafety
interlocksensorandTRILUTIONmicro.Thecontactscanalsobe
usedto
 controlperipheraldevices.Refertothediagramforthe
locationoftheinput/outputports.
ContactInputs–ThebottomterminalblockonthePPMXhas
twopairedinputcontactsthatarelabeled4and5.Never
connectvoltageshigherthan5VDCtoaninput.Whenusing
TTLsignals,besuretomatchGROUNDconnections.

ContactOutputs–ThebottomterminalblockonthePPMXhastwopaired,isolated‐relaycontactclosuresthat
arelabeled1and2.
DCPowerOutput–Thetopterminalblockhasone+12VDCoutput,whichislabeled.
ThePPMXhasonefunctionalUSBhostport(themiddleport)ontherearpanel.Thetopportontherearpanelis
not
 supported.TheUSBportscanbeusedtoconnectUSBdrives(whichcanalsobeseenbythetabletwhen
pluggedintothePPMX),
 keyboard,mouse,oranyothercompatibledevice(heater/chiller,barcodescanner,or
shaker,forexample).
TheUSBdeviceportisusedforcommunicationbetweenthePPMXandthetabletorcomputer.AUSBcableis
providedasastandardaccessory.RefertothediagramforthelocationoftheUSBdeviceport.
TheEthernetPort,RS‐232andHDMIarenotsupported.

ASSURANCEGDS®PPMXUSERMANUAL10
1 Input/Output
2 USBhost(NotSupported)
3 USBhost
4 Ethernet(NotSupported)
5 USBdevice
6 RS‐232(NotSupported)
7 HDMI(NotSupported)
8 Powerreceptacle
9 Powerswitch(MAINS)
Usethepowercordontheexternalpower  supplytomaketheconnectionbetweenthe  powerreceptacleonthe
PPMXandthe
 externalpowersupply.Theconnectionfromtheexternalpower  supplytothePPMXusesa
connectorwith
 alockingcollar.Checkthealignmentofthe  pinsandthenpushitinuntilitclicksandlocks  in
place.Todisconnect,pullbackonthe
 lockingcollarandthendisconnectthecable  fromtherearpanelofthe
PPMX.Locatetheappropriatepowercordforyourlinevoltageandthenmaketheconnectionbetweenthe
externalpowersupplyand
 theACpowersource.













ASSURANCEGDS®PPMXUSERMANUAL11
FrontPanelConnections
ThePPMXhastwofunctionalUSBhostportsonthefrontpanel.TheUSBportscanbeusedtoconnectUSB
drives(whichcanalsobeseenbythetabletwhenpluggedintothePPMX),
 keyboard,mouse,oranyother
compatibledevice(heater/chiller,barcodescanner,orshaker,forexample).

Inasituationwhereanemergencystopisrequired,pressingtheSTOPbuttonstopsthePPMXimmediately.The
protocol
 stopsandresultsaredisplayed(aftertouchingOKonthenotification).

1 USBhost
2 STOP

PowerOnandStartUp
TostartthePPMX:

1 MakesurethatthePPMXisconnectedtotheexternalpowersupplyandthattheexternalpowersupplyis
connected
 toapowersource.
2 Turnpoweronattheexternalpowersupply.
3 TurnthePPMXpoweronusingtheMAINSpowerswitchlocatedontherearpanel.Theindicatorlighton
thefrontpanel
will illuminategreen.
4 ConnectthecontroltablettothePPMXandpoweriton.
5 Closetherotatingcoverdoor.ThePPMXwillnotoperatewiththecoverorhoodopen.

TouchscreenTabletOverview
ThefollowinginformationprovidesanexplanationofuniquesoftwareinteractionswhenrunningTRILUTION
microonthe
 suppliedtouchscreencontroltablet.Itisrecommendedtokeepthetabletpluggedintoapower
sourcewheneverpossible.CommunicationerrorscanoccurbetweenthePPMXandcontroltabletifthetablet
batteryistoolow.

Refertothetablebelowforadescriptionoftheiconsusedinthe
 on‐screenkeyboard.







ASSURANCEGDS®PPMXUSERMANUAL12
ThefollowingiconsappearonseveralscreensinTRILUTIONmicro.
Toviewtheoptionstoshutdownthecontroltabletand/orTRILUTIONmicro,touchonthemainmenu:

• TouchShutdowntocloseTRILUTIONmicroandshutdownthecontroltablet.
• TouchRestartTRILUTIONmicrotorestartonlythesoftware,andnotthecontroltablet.
• TouchCanceltogobacktothemainmenu.

AlignmentProtocol
RuntheAlignmentProtocoltoensureproperalignmentofthePPMX.Itisonlynecessarytorunthisprotocol
whensetting
 uptheinstrumentforthefirsttimeorifanalignmentissueissuspected.

1 Passtheendofthealignmentcablecordwithpre‐wired
connectorthroughthe
 outletatthebackofthePPMX.
2 TheconnectorislabeledBOTTOMbecauseitconnectstothe
bottomsetof
 input/outputportsontherearpanelofthe
PPMX.Maketheconnection.
3 Theclipstosecurethetipracksonthetraycaninterferewiththealignment.Ensure
thattheclips()arepositionedasshowninthediagram
below,andthatthe
 thumbscrewshavebeentightened.






4 Closetherotatingcoverorthefumehooddoor.
5 ThetabletstartsTRILUTIONmicroautomaticallyonpowerup.
6 TouchRunaprotocol.
7 SelectAlignmentProtocolandthentouchNext.


ASSURANCEGDS®PPMXUSERMANUAL13
8 TheScanningdialogappearswhilethesoftwarechecksthat the
PPMXis
 connected.Ifconnected,thesoftwaregoestothenext
screen.Ifnotconnected,a
 messagewillappearsuggesting
possiblesolutions.
9 TouchSkipsetuponLabwaresetupguide.
10 TouchRunprotocol.
11 Waitapproximately3minuteswhiletheAlignmentProtocolruns.
12 AnotificationappearswhentheAlignmentProtocoliscomplete.
TouchContinue.

LogIn
1 TouchHometogotothemainmenu.
2 TouchLogin.
3 EntertheUserIDandpasswordandaftereachentryusingtheon‐screenkeyboardon  thetablet,andthen
touchAccept(ortheXtocancel).

TheUserIDis
 AdminandthepasswordisGilson268

AddPipetteHead(s)
Eachpipetteheadthatmaybeusednow,orinthefuture,mustbeaddedpertheinstructionsthatfollow.

1 OntheTRILUTIONmicromainmenu,touchSettings.
2 OntheSettingsmenu,touchPipetteheads.
3 TouchScanandthenfollowtheon‐screendirectionstousethetabletcameratoscan  thebarcodeonthe
pipettehead,orenterthevaluesfromthedocumentation
 suppliedwitheachpipettehead.








4 TouchSave.
5 Repeatsteps3and4foradditionalpipetteheads.Whenfinishedaddingheads,  touchBack.

ASSURANCEGDS®PPMXUSERMANUAL14
SetDate&Time
Setthedateandtimetoensureacorrectdate/timestamponprotocol
runs.Thisshould
 onlyhavetobedoneonce.OntheSettingsmenu,touch
Date/time,andthensetthedate,time,andtimezone.

CreateUsers
Createuserstocontrolaccesstodifferentareasofthesoftware.  Togoto
theUsersscreen,touchUsersonthemainmenu.TheUsersscreen
displaysalistofusers.Onthisscreen,auserwhoisanadministratorcan

create,view,edit,anddeleteusers.

Refertotheinformationbelowtoaddusers.Forinformationabouthow
todeleteusersor
 modifyuserinformation,viewthehelpinformationin
thesoftware,fromtheaccessiblebutton.
UserIDisusedwhentheuserlogsin.
Fullnameisthefullnameoftheuser.
Admin?isusedtoassignadministrativeaccesstoauser.AnAdmincan
add,delete,and
 modifyusers,deleteorreplaceprotocols,deleteresults,
changepipetteheads,andaccess
 thesettingsoptions.
Changepasswordisusedtochangeorassignthepasswordthatis
requiredwhenthe
 userlogsin.

Toaddauser,touchAddandanewrowwillappear.

1 TouchintheUserIDfieldtoassignauserID.Theonscreenkeyboardwillappear.
a) UsetheonscreenkeyboardtotypetheUserID.
• TheUserIDcannotbethesameasanyotherUserID.
• TheUserIDcanbefrom1to24characters.
• TheUserIDcanuseanycharacterthatappearsontheonscreenkeyboard.
b) TouchthecheckmarktoaccepttheUserIDandclosethekeyboardortouchXtoclosethekeyboard
withoutmakingany
 changes.

2 TouchintheFullnamefieldtoassignafullnamefortheuser.Theonscreenkeyboardwillappear.
a) UsetheonscreenkeyboardtotypetheFullname.
• TheFullnamecanbefrom1to80characters.
• TheFullnamecanuseanycharacterthatappearsontheonscreenkeyboard.
b) TouchthecheckmarktoaccepttheFullnameandclosethekeyboardortouchXtoclosethe
keyboardwithoutmaking
 anychanges.

3 TouchintheAdmin?fieldtotoggleadministrativeaccessonoroff.Acheckindicatestheuserisan
administrator.







ASSURANCEGDS®PPMXUSERMANUAL15
4 TouchChangepasswordtoassignapasswordfortheuser.Thechangepasswordscreenwillappear.
a) TouchintheEnterpasswordfield.Theonscreenkeyboardwillappear.
b) Usetheonscreenkeyboardtotypethepassword.
• Thepasswordcanbeanylength.
• Thepasswordcanuseanycharacterthatappearsontheonscreenkeyboard.
• Thepasswordiscase‐sensitive.
• Thepasswordisrequiredandcannotbeblank.
c) TouchthecheckmarktoacceptthepasswordandclosethekeyboardortouchXtoclosethe
keyboardwithoutmaking
 anychanges.
d) TouchintheRe‐enterpasswordfieldandre‐typethepassword.
e) TouchAccepttoacceptthepasswordandclosethechangepasswordscreen.Bothpasswordsmust
matchtobeaccepted.
 Or,touchXtoclosethechangepasswordscreenwithoutmakinganychanges.
RunaProtocol
TheLabwaresetupguidescreendisplaystheoptionsforconfirmingtheinstrumentsetup.
SelectStep‐by‐stepwizardtodoallofthefollowing(intheordershown):
• Reviewalistofthematerials.
• Reviewthelayoutofthetrayandsetuppipettehead.
• Setupthepipette,tiprackandadditionallabware.
• Reviewprotocol/deck.

Fordetails,refertoStep‐by‐stepWizardonthenextpage.




SelectBrowsepositionsmanuallytodoanyofthefollowing:
• Reviewthelayoutofthetrayandreviewpipettehead.
• Selectandsetupspecificlabware.
• Reviewprotocol/deck.

SelectSkipsetuptoskipsetupinstructionsandgototheWizardcompletescreen.Protocolwillbesettothe
default
 valuesorthevaluesusedthelasttimetheprotocolwasrun.Thisincludesthenumberofsamplesand
tipsinthereloadblock.
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ASSURANCEGDS®PPMXUSERMANUAL16
Step‐by‐stepWizard
1 Touchtogotothemainmenu.
2 TouchRunaprotocol.
3 Touchtoselectyourprotocol,andthentouchNext.
4 TheScanningdialogappearswhilethesoftwarechecksthatthe
PPMXis
 connected.Ifconnected,thesoftwaregoestothenext
screen.Ifnotconnected,a
 messagewillappearsuggesting
possiblesolutions.
5 Entersamplenumber,kitlotnumber,andreagentlotnumbersand
thentouchNext.
6 TouchStep‐by‐stepwizard.
7 GatherthematerialsintheMaterialsListandthen  touchNext.
8 Waitforthesoftwaretofinish“Preparingpipettehead”andthen
opentherotatingcover
 orthehooddoor.

Steps9–11onlyneedtobecompletedduringinstallationorafter
runningthealignmentprotocol.
9 Removethealignmentheadbydisconnectingtheterminalblock
connectorfromtherear
 panel,andthenremovingthethumbnuts
securingittotheupperpipetteheadassembly.
 
10 Installthepipetteheadontheleftupperpipetteheadassembly
usingthe
 thumbnuts.Ensureproperalignmentofthepipettehead
againsttheupper
 pipetteheadassemblybeforetighteningthe
thumbnuts
11 Selecttheserialnumberthatmatchestheinstalledpipettehead
fromthedrop‐downlist,close
 therotatingcover,andthentouch
Next.
12 ProceedthroughtheTipsetupandLabwaresetupscreensbyreviewingtheinformation,makingany
desired
 changes,andthentouchingNext.Thescreensindicatethelabwaretouseandwheretoplaceiton
thetray.

Eachtiprackconsistsofatippackandatipreloadblock.
 To
assemblethetiprack:

1 Openthesealedblisterpack.
2 Liftthetippackoutoftheblisterpack.
3 Setthetippackonthetipreloadblock.Ensurethatthe
tippackisfullyseatedon
 thetipreloadblock.
4 Removetheclearcover.

13 Toinstallthetiprack:
1 Loosenthethumbscrewforeachclip.
2 Placethetiprackontheremovabletraywiththebeveledcornerinthe  backleftcorner.
3 Rotateeachclipsothatitwilleffectivelysecurethetiprackonthetrayasshown  intheimage
below.
4 Tightenthethumbscrewforeachclip.

ASSURANCEGDS®PPMXUSERMANUAL17
14 Reviewprotocol/deckandthentouchNext.
15 TouchRunprotocol.
16 Whenprompted,itisrecommendedtosimulateifthisisthefirsttimerunningthe  selectedprotocol.


PauseorStopaRunningProtocol
WhilerunningaprotocolfromTRILUTIONmicro,touchanywhereonthePIPETSTATUS  imagetopause
therun.Therunwillstopwhenitfinishesitscurrentaction.Theprotocol
 runtimerkeepsadvancing
duringthepause.Whenprompted,touchContinueto
 resumetherun,orStoptoendtherunandview
theresults.

Donotpausetheprotocolwhilepipettetipsarebeingpickeduporejected.

TostopthePPMXinanemergency,presstheredSTOPbuttononthefrontofthePPMX.
ViewResults
Duringandafteraprotocolrun,informationisavailableaboutreagentdispensevolumes.Afteraprotocolrun,
resultsforthatrunareautomaticallydisplayed.Toviewresults:
1 Touchtogotothemainmenu.
2 TouchResults.
3 Touchtoselectaprotocol,andthentouchResults.
4 Touchtoselectaprotocolrun,andthentouchView.Simulatedrunsare
identified
 by.
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ASSURANCEGDS®PPMXUSERMANUAL18

5 ThefirstscreendisplayedwhenviewingresultsistheTrayviewscreen.Thecolorsindicatethefollowing
information:

Red–Negativevolume
Green–Volumeinthetubeorwell
Blue–Emptytubeorwell
Pink–Tips

6 Thetopofthescreenshowsthestatus,thenameoftheprotocol,thetimeittooktorunorsimulatethe
protocolandgenerate
 theresults,andthetimeofexecutionforthelaststep.
7 TheVolumeviewscreendisplaysinformationaboutthevolumeintheselectedwellorreservoir.
8 Thetopofthescreenshowsthestatus,thenameoftheprotocol,thetimeittooktorunorsimulatethe
protocolandgenerate
 theresults,andthetimeofexecutionforthelaststep.
9 Thetextabovethetableliststhebedelementname,thewelllocationandlabel,andthecurrentvolumein
thewell.Thetableliststheactionsthatoccurredinthewellorreservoirinthefollowingorder:

Initialvolume(ifany)
Volumeaddedandthesourceofthevolumeadded(bedelementnameandplateindexorreservoir
number)
Volumeremoved

10 TheStepsviewscreendisplaysthestepsthatwererunintheprotocolintheorderof  execution.
11 Thetopofthescreenshowsthestatus,thenameoftheprotocol,thetimeittooktorunor  simulatethe
protocolandgeneratetheresults,andthetimeofexecutionforthelaststep.

RunReport
TheRunReportisasubsetoftheresultsandcanbeexportedfor  viewing.Itcannotbeviewedfromwithinthe
softwareoronthetablet.Itincludesbasicinformationabouttheprotocolrun,thebedlayout,
 andwelltracking.It
doesnotincludetheinformationaboutvolumetracking.

1 Touchtogotothemainmenu.
2 TouchResults.
3 Touchtoselectaprotocol,andthentouchResults.
4 Touchtoselectaprotocolrun,andthentouchExport.
5 Selectthefiletypefortheexportedfile:.htm(default),.xmlor.csv.
6 BrowseforandselecttheUSBdrive,namethefile(oracceptthe
defaultname,which
 isthenameoftheprotocol),andthentouch
Save.TheRunReportissavedtothelocation.
7 Openthefileonacomputer.

ASSURANCEGDS®PPMXUSERMANUAL19
ImportProtocol
Toimportaprotocol:

1 Touchtogotothemainmenu.
2 ConnectaUSBdrivecontainingtheprotocolfiletooneoftheUSBports
onthefrontofthePPMXorthemiddleUSBhost
 portontherearpanel
(thetopUSBportontherearpanelisnotsupported).
3 TouchManageprotocolstogotothelistingofprotocols.
4 TouchImportandthenbrowseforyourapplicationprotocol
file(.sqlite)onthe
 USBdrive.
5 SelectthefileandthentouchOpentoimporttheprotocolfile.

ExportProtocol
Toexportaprotocol(anditsresults):

1 Touchtogotothemainmenu.
2 TouchManageprotocolstogotothelistingofprotocols.
3 Touchaprotocolinthelist.Itwillhighlightwhenselected.
4 TouchExport.
5 Browseforandselectthedestination(USBdrive)fortheprotocolfile,andthentouchOpen.  Theprotocolis
savedtothelocationwitha.SQLITEextension.

ChangeSerialNumberinTabletSoftware
TocontrolaPPMXfromTRILUTIONmicro,theserialnumberofPPMXmustbeenteredintoTRILUTIONmicro
software.WhenTRILUTIONmicroisfactory‐installedonthesuppliedtablet,theserialnumberofthe
correspondingPPMXisenteredintothesoftware.Ifinstallingareplacementtablet,followtheinstructionsbelow.

1 GototheHOMEscreen.
2 ClickSettings.
3 EnterAdminUserIDandPassword.
4 ClickProtocolsettings.
5 EntertheSerialnumberforPPMX.
6 ClickBackandreturntotheHOMEscreen.
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