cytiva Amersham ImageQuant 500 User manual

Amersham ImageQuant 500
Operating Instructions
Original instructions
cytiva.com

Table of Contents
1Introduction ........................................................................................................ 4
1.1 Important user information ....................................................................................................................... 5
1.2 About this manual ......................................................................................................................................... 6
1.3 Associated documentation ....................................................................................................................... 7
2 Safety instructions ............................................................................................. 8
2.1 Safety precautions ........................................................................................................................................ 9
2.2 Labels and symbols ....................................................................................................................................... 16
2.3 Emergency procedures ............................................................................................................................... 18
3 System description ............................................................................................ 20
3.1 Feature overview ............................................................................................................................................ 21
3.2 Hardware .......................................................................................................................................................... 22
3.3 Start screen and capture tab ..................................................................................................................... 24
3.4 Accessories ...................................................................................................................................................... 28
4 Installation .......................................................................................................... 29
4.1 Site requirements .......................................................................................................................................... 30
4.2 Unpacking ......................................................................................................................................................... 31
4.3 Instrument set up .......................................................................................................................................... 34
4.4 Connection to network or USB flash drive ............................................................................................ 38
4.5 Moving the ImageQuant 500 system ..................................................................................................... 42
5 Operation ............................................................................................................ 44
5.1 Start the system ............................................................................................................................................. 45
5.2 Operation overview ....................................................................................................................................... 47
5.3 Place the sample on a tray .......................................................................................................................... 48
5.4 Capture an image .......................................................................................................................................... 51
5.4.1 Chemiluminescence capture .................................................................................................................. 52
5.4.2 Fluorescence capture ................................................................................................................................. 71
5.4.3 Colorimetric capture ................................................................................................................................... 84
5.4.4 Set the exposure and interval time ....................................................................................................... 87
5.5 View the images ............................................................................................................................................. 89
5.6 Save the image ................................................................................................................................................ 94
5.7 File handling ..................................................................................................................................................... 97
5.8 Software keypad ............................................................................................................................................. 102
5.9 Shutdown .......................................................................................................................................................... 103
6 System settings .................................................................................................. 104
6.1 Introduction to the Settings window .................................................................................................... 105
6.2 Save location ................................................................................................................................................ 107
6.3 Date and time .................................................................................................................................................. 110
6.4 Network ............................................................................................................................................................. 111
6.5 Maintenance .................................................................................................................................................... 112
Table of Contents
2 Amersham ImageQuant 500 Operating Instructions 29655392 AA

7 Maintenance ....................................................................................................... 114
7.1 Safety precautions ........................................................................................................................................ 115
7.2 Cleaning before planned service ............................................................................................................. 116
7.3 Cleaning the exterior .................................................................................................................................... 117
7.4 Cleaning sample trays and filter ............................................................................................................... 118
7.5 Regular inspections ...................................................................................................................................... 119
8 Troubleshooting ................................................................................................. 120
8.1 Report an error ................................................................................................................................................ 121
8.2 Problems with the captured image ......................................................................................................... 122
8.3 Strange sounds and odors .......................................................................................................................... 123
8.4 Problems with ImageQuant 500 ............................................................................................................. 124
8.5 Problems with ImageQuant 500 Control Software .......................................................................... 125
9 Reference information ....................................................................................... 126
9.1 Specifications .................................................................................................................................................. 127
9.2 Environmental conditions .......................................................................................................................... 128
9.3 Recycling information .................................................................................................................................. 130
9.4 Regulatory information ............................................................................................................................... 132
9.4.1 Contact information .................................................................................................................................... 133
9.4.2 European Union and European Economic Area .............................................................................. 134
9.4.3 Great Britain ................................................................................................................................................... 135
9.4.4 Eurasian Economic Union
Евразийский экономический союз ................................................................................................... 136
9.4.5 Regulations for North America ............................................................................................................... 138
9.4.6 Regulatory statements .............................................................................................................................. 139
9.4.7 Declaration of Hazardous Substances (DoHS) ................................................................................ 140
9.4.8 Other regulations and standards .......................................................................................................... 142
9.5 Health and Safety Declaration Form ...................................................................................................... 143
Index ........................................................................................................................... 145
Table of Contents
Amersham ImageQuant 500 Operating Instructions 29655392 AA 3

1 Introduction
About this chapter
This chapter contains information about this manual and associated user
documentation, important user information and intended use of the product.
In this chapter
Section See page
1.1 Important user information 5
1.2 About this manual 6
1.3 Associated documentation 7
1 Introduction
4 Amersham ImageQuant 500 Operating Instructions 29655392 AA

1.1 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing,
operating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not install, operate, or perform maintenance on the product in any other way than
described in the user documentation. If you do, you may be exposed or expose others
to hazards that can lead to personal injury and you may cause damage to the
equipment.
Intended use of the product
Amersham™ ImageQuant™ 500 is a CCD camera system that produces digital images
of chemiluminescent and fluorescent samples or stained gels and membranes.
ImageQuant 500 is intended for research use only, and shall not be used in clinical
procedures, or for diagnostic purposes.
Prerequisites
In order to operate ImageQuant 500 in the intended way, the following prerequisites
must be fulfilled:
•You have read and understood the safety instructions outlined in this Operating
Instruction
•You should be acquainted with the use of general laboratory equipment and with
handling of biological materials
•The instrument is installed according to the instructions in this Operating
Instruction
1 Introduction
1.1 Important user information
Amersham ImageQuant 500 Operating Instructions 29655392 AA 5

1.2 About this manual
Purpose of this manual
The Operating Instructions manual provides information needed to install, operate and
maintain the product in a safe way.
Scope of this manual
This Operating Instruction manual is valid for ImageQuant 500. The illustration below
shows the ImageQuant 500 system.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
In electronic format, references in italics are clickable hyperlinks.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your
procedures.
1 Introduction
1.2 About this manual
6 Amersham ImageQuant 500 Operating Instructions 29655392 AA

1.3 Associated documentation
Introduction
This section describes the user documentation delivered with the product, and how to
find related literature that can be downloaded or ordered from Cytiva.
User documentation for ImageQuant
500
The user documentation is listed in the table below.
Translations of the Operating Instructions are provided on the User Documentation CD
(or USB flash drive) together with the Privacy and Security Manual.
Documentation Main contents
Amersham ImageQuant 500 Operating
Instructions
(this document)
Instructions needed to prepare and
operate the ImageQuant 500 system in
a correct and safe way.
System overview, site requirements,
and instructions for moving the system
within the same building.
Instructions for basic maintenance and
troubleshooting.
Amersham ImageQuant 500 Privacy
and Security Manual
Descriptions of the privacy and security
aspects of ImageQuant 500 system.
Amersham ImageQuant 500 Site
Preparation Guide
Information needed to prepare the site
for installation and use of the
ImageQuant 500 system.
User documentation and other
literature on the web
User documentation and other literature related to ImageQuant 500 may be
downloaded from the web. Follow the steps below to access the documentation.
Step Action
1Go to cytiva.com/imagequant.
2Navigate to RELATED DOCUMENTS.
3Select the type of document and download the chosen literature.
1 Introduction
1.3 Associated documentation
Amersham ImageQuant 500 Operating Instructions 29655392 AA 7

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures.
In this chapter
Section See page
2.1 Safety precautions 9
2.2 Labels and symbols 16
2.3 Emergency procedures 18
Important
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
2 Safety instructions
8 Amersham ImageQuant 500 Operating Instructions 29655392 AA

2.1 Safety precautions
Introduction
ImageQuant 500 is powered by mains voltage and handles materials that can be
hazardous.
Before installing, operating or maintaining the system, you must be aware of the
hazards described in this manual.
Follow the instructions provided to avoid personal injuries, damage to the
product, or to other personnel and equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Installing and moving the system
•System operation
•Maintenance
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
2 Safety instructions
2.1 Safety precautions
Amersham ImageQuant 500 Operating Instructions 29655392 AA 9

General precautions
WARNING
Do not use the equipment if smoke, strange noises or strange
odors can be perceived, or if the equipment becomes unusually
hot. This may result in fire or electric shock.
Stop using the equipment immediately, turn off the power switch
and unplug the equipment from the power outlet. Contact your
local Cytiva representative to request repair.
WARNING
Do not damage the power supply cord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local Cytiva
representative for replacements.
WARNING
Access to power switch and power cord with plug. Do not
block access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.
CAUTION
Use of this equipment in other ways than those specified in the
user documentation may result in hazardous UV radiation
exposure.
CAUTION
Do not block the vents and ensure that the vents are kept free of
dust and dirt. Blockage of the vents can result in overheating of the
equipment and malfunction. Place the instrument so that there is
at least 20 cm in front of the machine to the walls or other
equipment, and 10 cm on all other sides to ensure adequate
cooling.
2 Safety instructions
2.1 Safety precautions
10 Amersham ImageQuant 500 Operating Instructions 29655392 AA

NOTICE
This equipment is for laboratory use only.
Personal protection
CAUTION
Parts of this equipment may emit UV radiation. Avoid exposure.
Wear protective clothing and eyewear. UV radiation can cause
severe burns and long-term injury to the skin and eyes.
CAUTION
If the door is opened and the interlock has been tampered with, UV
and visible light will be emitted.
Do not stare at the light emitted from the light sources. This may
impair vision.
CAUTION
Always wear gloves, protective glasses and a lab coat or similar
when handling samples.
Installing and moving the instrument
WARNING
Use a proper power cord that complies with the local laws and
regulations, and is delivered by Cytiva. Do not change power cord
to inappropriate one.
2 Safety instructions
2.1 Safety precautions
Amersham ImageQuant 500 Operating Instructions 29655392 AA 11

WARNING
In USA and Europe (Belgium, Netherlands, Luxemburg, Germany,
France, Italy, Denmark, Greece, Spain, Portugal, Austria, Finland,
Sweden, Poland, Hungary, Czech, Slovenia, Slovakia, Estonia,
Latvia, Lithuania, and Romania), use the proper power cord
included in the product package. In other countries, use the proper
power cord delivered separately from the product package by
Cytiva. Do not change AC power cord to inappropriate one.
WARNING
Connect the power supply directly to a grounded wall power outlet.
The use of extension cords or multiple loads on one electrical outlet
could result in fire and electric shock.
CAUTION
Do not place the equipment on unstable tables or on inclined
surfaces, as the equipment could be tipped over or fall, resulting in
injury.
CAUTION
Do not place the equipment or parts in direct sunlight. This may
degrade performance.
CAUTION
Turn off the power switch and remove connecting cables before
moving the equipment.
NOTICE
Do not connect telephone lines to the Ethernet port.
Only the EN/IEC 62368-1 standard non-shielded cables are
appropriate for connection to this connector.
2 Safety instructions
2.1 Safety precautions
12 Amersham ImageQuant 500 Operating Instructions 29655392 AA

System operation
WARNING
Do not use the equipment within or near a sink, or in humid or dusty
environments. Fire and electric shock could result.
WARNING
If thunder can be heard, do not touch the power supply plug, or
electric shock could result.
CAUTION
Reagents used to prepare the sample should be used in
accordance with the manufacturer instructions.
CAUTION
Do not lean on the sample door, it might cause breakage to the
equipment or the machine to tip over and injure you.
CAUTION
When opening or closing the door use the handle. Take care not to
catch objects or fingers in the door when closing.
CAUTION
Do not place heavy objects on the equipment. These may fall and
cause injury.
CAUTION
Do not turn the power off during image capture and saving. This
may damage the system files.
2 Safety instructions
2.1 Safety precautions
Amersham ImageQuant 500 Operating Instructions 29655392 AA 13

CAUTION
Do not leave samples in the equipment after exposure. If left, these
may degrade and cause damage.
CAUTION
Remove all traces of acidic sample solutions to prevent corrosion in
the equipment.
NOTICE
Avoid touching the windows of the light sources and the lens. This
may degrade performance.
NOTICE
Do not scratch or drop the filter. This may degrade performance.
NOTICE
When using incremental exposure mode, images that are not
selected will be deleted at saving.
Maintenance
WARNING
Do not attempt to modify the equipment, or fire and electric shock
could result.
WARNING
Do not use excessive amounts of liquids for cleaning the
ImageQuant 500, this may result in product malfunction or electric
shock.
2 Safety instructions
2.1 Safety precautions
14 Amersham ImageQuant 500 Operating Instructions 29655392 AA

CAUTION
Wear gloves to prevent direct contact with chemical substances.
CAUTION
Turn the power switch off before cleaning the inside of the
equipment.
CAUTION
Unplug the equipment if it will not be used for an extended period.
CAUTION
Take care when connecting the power cord. Do not tug at the
power cord, and do not handle the connection plugs with wet
hands.
CAUTION
Do not press too hard onto the surface of the touch screen. This
may cause the surface to break and lead to injury.
NOTICE
Do not use abrasive cleaning materials, such as a scouring pad, for
cleaning. This may scratch the surface.
2 Safety instructions
2.1 Safety precautions
Amersham ImageQuant 500 Operating Instructions 29655392 AA 15

2.2 Labels and symbols
Introduction
This section describes the product nameplate (label) and other safety and regulatory
labels attached to the product.
System label
The system label is located on the back of the equipment. The system label identifies
the equipment and shows electrical data, regulatory compliance, and warning
symbols.
Description of symbols on the system
The following symbols and text may be present on the system:
Symbol / text Description
Warning! Read the user documentation before using the system. Do not open
any covers or replace parts unless specifically stated in the user documentation.
This symbol indicates the connection point for the USB flash drive on
ImageQuant 500
Frequency
Supply Voltage
Max. Power
Electrical requirements:
•Frequency (Hz)
•Supply Voltage (VAC )
•Max. Power (W)
Alternating current.
Protection
Class
Degree of protection provided by the enclosure.
Manufacturing
Year and Month
Year (YYYY) and month (MM) of manufacture.
2 Safety instructions
2.2 Labels and symbols
16 Amersham ImageQuant 500 Operating Instructions 29655392 AA

Position of labels and symbols
Part Description
1 USB label
2 System label
3 FCC and ICES-003 label
2 Safety instructions
2.2 Labels and symbols
Amersham ImageQuant 500 Operating Instructions 29655392 AA 17

2.3 Emergency procedures
Introduction
This section describes how to shut down the ImageQuant 500 in an emergency
situation, and the procedure for restarting the system.
The section also describes the result in the event of power failure.
Precautions
WARNING
Access to power switch and power cord with plug. Do not
block access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.
Emergency shutdown
In case of emergency:
Step Action
1Turn off the power by pushing the Power switch to the O position on the
bottom right hand side of the instrument.
2Disconnect the power cord from the wall socket.
Power failure
The following table describes the consequences of a power failure.
2 Safety instructions
2.3 Emergency procedures
18 Amersham ImageQuant 500 Operating Instructions 29655392 AA

Power failure to... will result in...
ImageQuant 500
instrument
•The run is interrupted immediately
•Any image capture in process will be lost. Only saved data
will be available on reboot.
•The Power indicator switches off.
Computer •The Power indicator on the Instrument control panel
displays blue.
•The run is interrupted immediately.
•If a run was in process, no image data will be saved.
2 Safety instructions
2.3 Emergency procedures
Amersham ImageQuant 500 Operating Instructions 29655392 AA 19

3 System description
About this chapter
This chapter contains information on the ImageQuant 500 hardware
components,accessories, start screen, and a feature overview.
In this chapter
Section See page
3.1 Feature overview 21
3.2 Hardware 22
3.3 Start screen and capture tab 24
3.4 Accessories 28
3 System description
20 Amersham ImageQuant 500 Operating Instructions 29655392 AA