Biocontrol Assurance GDS PPMX User manual

55240.R003.082016
ASSURANCEGDS®PPMX
USERMANUAL
 
12822SE32ndStreet
Bellevue,WA,USA98005
Phone425.603.1123
Fax425.603.0070
[email protected]
www.biocontrolsys.com

ASSURANCEGDS®PPMXUSERMANUAL1
TableofContents
I. Safety .................................................................................................................... 4
Voltage
SafetyInterlock
PipetteHeads
TipWasteContainer
Tray
Liquids
ReplacementParts
II. Introduction .......................................................................................................... 6
StandardEquipment
Documentation
Protocols
Disposables
TechnicalSpecifications
III. Setup/Operation................................................................................................... 9
Unpacking
RemovableTray
RearPanelConnections
FrontPanelConnections
UPSBatteryPowerandLineConditioner
PowerOnandStartUp
LogIn
UserManagement
TouchscreenOverview
AlignmentProtocol
AddPipetteandPickPenHead(s)
SetDate&Time
InstallChimeSoundBox
RunaProtocol
Step‐by‐stepWizard

ASSURANCEGDS®PPMXUSERMANUAL2
PauseorStopaRunningProtocol
ViewResults
RunReport
LogOut
LIMSCompatibility
ImportProtocol
ExportProtocol
DeleteProtocol
ChangePPMXSerialNumberinSoftware
IV. Troubleshooting.................................................................................................. 23
CommunicationLoss
Re‐startingaProtocolafterCommunicationLoss
HoodOpen
ReportinganError
TechnicalServices
V. Maintenance....................................................................................................... 25
Cleaning
PipetteHeadCalibration
AlignmentCalibration
PreventativeMaintenance
Warranty
InstrumentReturnProcedure
VI. AppendixA.......................................................................................................... 27
PartsList
VII. AppendixB.......................................................................................................... 29
BeforeYouCallUs
VIII. AppendixC.......................................................................................................... 30
MaintenanceLog
IX. AppendixD...........................................................................................................31
WarrantyTerms
X. AppendixE.......................................................................................................... 32
PipetteVerification
DeckLayoutforR‐StandardLowMicroCalibration
Verification
XI. AppendixF.......................................................................................................... 34

ASSURANCEGDS®PPMXUSERMANUAL3
BestPractices
XII. AppendixG.......................................................................................................... 35
PackUp
XIII. AppendixH.......................................................................................................... 39
OrderingInformation

ASSURANCEGDS®PPMXUSERMANUAL 4
Safety
ReadthissectionbeforeinstallingandoperatingtheAssuranceGDS®PPMX.

Thisinstrumentisintendedtobeusedinalaboratoryenvironmentbytrainedtechnicalpersonnel.

Forsafeandproperuseofthisinstrument,itisrequiredthatbothoperatingandservicepersonnelfollowthe
instructions
 containedinthisguidewheninstalling,operating,cleaning,andmaintainingtheinstrument.

Thefollowingsafetyprecautionsmustbeobservedduringallphasesofoperation,service,andrepairofthe
instrument.Failure
 tocomplywiththeseprecautionsorwithspecificwarningselsewhereinthisuser’sguide
violatessafetystandardsofdesign,
 manufacture,andintendeduseoftheinstrument.BioControlSystems
assumesnoliabilityforthecustomer’sfailuretocomplywiththese
 requirements.
ThePPMXhasbeencertifiedtosafetystandardsrequiredinCanada,Europe,andtheUnitedStates.Refertothe

instrumentrearpanellabelandtheDeclarationofConformitydocumentforthecurrentstandardstowhichthe
instrumenthas
 beenfoundcompliant.
Thefollowingelectronicandhazardsymbolsmayappearontheinstrument:
Thefollowingsafetynoticesmayappearinthisdocument:
Voltage
Accesstotherearpanelisnecessarybecausetheinstrumentmustbedetachedfromallvoltagesourcesbefore
service,repair,
 orexchangeofparts.
Operatetheinstrumentusingtheapprovedpowersupplyprovidedandonlyatthevoltagespecifiedontherear
panellabelof
 theinstrument.

ASSURANCEGDS®PPMXUSERMANUAL 5
SafetyInterlock
ThePPMXisconfiguredwithasafetyinterlock,whichisusedwhentherotatingcoverisinstalledonthe
instrument.
Thesafetyinterlockpreventstheinstrumentfromoperatingwhentherotatingcoverisopen.

PipetteHeads
Theinstrumentiscapableofgeneratingsignificantforcesthatcouldcausepotentialinjurytotheuser.Foran
instrumentwith
 therotatingcoverinstalled,thesafetyinterlockinthecoverwilldisabletheinstrumentmovement
ifthecoverontheinstrument
 isopen.Foraninstrumentinstalledinahood,theexternalsafetyinterlocksensor
mountedontheinstrumentwilldisablethe
 instrumentmovementifthehooddoorisopen.
TipWasteContainer
Thetipwastecontainershouldbeemptiedinaccordancewithnationalandlocalsafetyregulations.

Tray
Themovingtrayiscapableofcausinginjurybypinching.Foraninstrumentwiththerotatingcoverinstalled,the
safetyinterlock
 inthecoverwilldisabletheinstrumentmovementifthecoverontheinstrumentisopen.
Liquids
Observesafelaboratorypracticeswhenhandlingliquids.Ifworkingwithbiologicalsamplesorchemicalsubstances,
ensurethat
 thereisproperventilation,andwearpersonalprotectiveequipment(PPE),suchassafetyglasses,
gloves,andprotectiveclothing
 atalltimes.

RefertotheSafetyDataSheetsforsolventsbeforeuse.

ReplacementParts
Besuretouseonlyreplacementpartsspecifiedinthisusermanual.Donotrepairorchangepartswhicharenot
listedinthis
 usermanual.Ifitisnecessarytochangepartsnotlisted,pleasecontactBioControlSystems
technicalservices.

ASSURANCEGDS®PPMXUSERMANUAL 6
Introduction
TheAssuranceGDSPickPen®PIPETMAX®(PPMX)isanautomatedmagneticparticlesandliquidhandler.Itis
capableofdispensingAssuranceGDSreagentswhichinclude,ConcentrationReagent,WashSolution,
ResuspensionBufferandselectmedia(BHI/DFB).ReagentdispenseprotocolshavebeencreatedforallAssurance
GDSassays.Inaddition,thePPMXhasalsobeenprogrammedtoautomateAssuranceGDSPickPen
ImmunomagneticSeparation(IMS)procedures.ThisusermanualisintendedtoassistPPMXuserswithsystem
start‐up,basicoperation,maintenanceandservice.
StandardEquipment
AfterthePPMXandaccessoriesareunpacked,thefollowingitemsshouldbepresent:

• PPMXInstrumentwithrotatingcover
• AlignmentHead
• RemovableTray96well
• PipetteTipReloadRack
• PickPenTipReloadRack
• TipDisposalBin
• PowerSupply(110V/220V)
• Power/USBCablingkit
• PowerCords(USandEU)
• USBCable
• BackPlug,Black
• AllenWrench,5mm
• MAX8x200PipetteHead
• AssuranceGDSPickPenMagneticHead
• AssuranceGDSReagentHolder
• TipMoverwithTipBoxHolder
• ConcentrationReagentBottleFillLevelSleeve
• ChimeSoundBoxwithhardware
• GelCoolingBlock(2/cs)





ASSURANCEGDS®PPMXUSERMANUAL 7
Systemalsocomeswitheither:

• ControlTabletwithTRILUTIONmicrosoftwarepre‐loaded+ChargerandTabletholder
or
• MicrosoftSurfacelaptopwithTRILUTIONmicrosoftwarepre‐loaded+ChargerandKeyboardcover/Pen

TheseitemscomewithUSplugsonly.Forpartnumbers,refertoAppendixA,PartsList.Forordering
information,refertoAppendixH,OrderingInformation.

Documentation
ThefollowingdocumentsareincludedwiththePPMX:

• PPMXUserManual
• GilsonQuickSetupGuide
• GilsonInstallationandOperationalQualification(IQ/OQ)Procedures
• GilsonDeclarationofConformity
• GilsonValidationCertificateforTRILUTIONmicro
• GilsonQualityControlReportPipetteHead

Protocols
• AlignmentProtocol
• AssuranceGDSReagentDispenseprotocols(R‐)
• AssuranceGDSPickPenIMSprotocols(IMS‐)
• AssuranceGDSCombinedReagentandIMSprotocols(C‐)

Disposables
Theitemsbelowaresoldseparatelybutarerequiredforuse:
• 200µLSterileBlisterFilterTipPacks
• ReagentReservoirs
• AssuranceGDSPickPenTips
• AdhesiveFilmSheets&Strips
• AssuranceGDSSampleWells&Bases
• ResuspensionPlates

Forpartnumbers,refertoAppendixA,PartsList.Fororderinginformation,refertoAppendixH,Ordering
Information.

ASSURANCEGDS®PPMXUSERMANUAL 8
TechnicalSpecifications
PleasebeawareofthefollowingbeforeoperatingthePPMX.

ChangesormodificationstothisdevicenotexpresslyapprovedbyBioControlSystems
couldvoidthewarranty.

Theinstrumentcomplieswithpart15oftheFCCRules.Operationissubjecttothefollowingtwoconditions:(1)
Thisinstrument
 maynotcauseharmfulinterference,and(2)thisinstrumentmustacceptanyinterference
received,includinginterferencethat
 maycauseundesiredoperation.

ShieldedcablesmustbeusedwiththeinstrumenttoensurecompliancewiththeFCCClassAlimits.

TechnicalSpecificationDefinition
CommunicationsUSB

ConnectionsThreeUSBhostportsandoneUSBdeviceport
Twoinputs(contactclosure,TTL),tworelayoutputs,andoneswitched+12VDC1Aoutput
NOTICE:Switchingvoltageshigherthan30Vorgreaterthan1Aofcurrentmaydamagethe
instrument.

Dimensions&WeightW54.4XD65.5XH53.1cm(21.4X25.8X20.9in),24.9kg(55lbs.)

EnvironmentalConditionsIndooruse
Altitude:upto2000m
Temperaturerange:5°C–40°C
Humidity:Maximumrelativehumidity80%fortemperaturesupto31°C,decreasinglinearity
to50%relativehumidityat40°C

PowerRequirementsExternalPowerSupply
VoltageInput
Frequency:50to60Hz
Voltage:100–240VAC
VoltageOutput
Voltage:24VDC
CurrentRating:6.25A,150W

FrontPanel&Control

TwoUSBhostportsandSTOPbutton,Touchscreentabletorlaptop
XYZMotion(Speed)1‐500mm/secinXdimension
1‐550mm/sec(350mm/secdefault)inYdimension(tray)
1‐140m/secinZdimension

IMSHead

PickPenmagnetichead

IMSTips

PickPentipcovers

LiquidContactMaterials

Tips–100%VirginPolypropylene
TipDisposalBin–Polypropylenewithcolorant(Pro‐fax6523)
LiquidHandlingHead&
Tips
MAX8x200,DIAMONDfiltertips(DF200)

RemovableTrayCapacity

9‐position
Safety&ComplianceCertifiedtosafetystandardsspecifiedforCanada,EuropeandtheUnitedStates.Refertothe
instrumentrearpanellabelandtheDeclarationofConformitydocumentforthecurrent
standardstowhichtheinstrumenthasbeenfoundcompliant.


ASSURANCEGDS®PPMXUSERMANUAL 9
Setup/Operation
TheAssuranceGDSPPMXanditscomponentsshouldbesetupintheorderdescribedinthissection.Gilson
TRILUTION®micro
 providessoftwarecontrolofthePPMXduringsetupandoperationasdescribedinthissection.
Formoreinformation
 aboutTRILUTIONmicro,viewthehelpinformationinthesoftware,accessiblefromthe
button.

Unpacking
ThePPMXisdeliveredwithmostmajorcomponentsalreadyassembled.Pleasekeeptheoriginalpackaging
(bothinternalboxesandtheexternalcrate)incasethePPMX
 needstobereturnedtoBioControlSystemsfor
repair.

Itisrecommendedthattwo
peopleliftthePPMXout
 ofthe
box,asitweighsapproximately55
lbs.(24.9kg).
1 Openthecratebyunscrewingthe4boltsatthebottom
ofthecrate(locatedjustabovethepallet).Usea9/16”
wrenchifneeded.Liftstraightupusinghandleson
bothsides.
2 Removethetwogreyfoampieces.
3 Removetheclosedandopen‐endedcardboard
accessoriesboxesbyslidingthemoutfromthebungee
cords.Becarefulnottodroptheboxeswhile
disassemblingthecords.
4 RemovetheplasticbagcoveringthePPMX.
5 GripthePPMXattherecessesnearthebase.Thereis
onerecessin
 thefront,oneintheback,andoneon
eachside.Usetheserecesses
 whenliftingthePPMX
outofthefoam‐linedtray.

DonotattempttoliftthePPMXfromthecoveror
fromtheX‐arm(thehorizontalarm).Alwayslifttheinstrumentfromitsbase.
6 PlacethePPMXonastable,levelsurfacesuchasalabbench,labcart.RefertoSection1:Safety.
7 Removethebluefilmorlabtapeprotectingtherotatingcover.
8 Removetheshippingbracefromtherearoftheinstrument.  Loosen
thethumbscrewssecuringtheshippingbracetothe
 PPMX.After
removingtheshippingbrace,replaceandtightenthe
 thumbscrews
onthebackoftheinstrument.







ASSURANCEGDS®PPMXUSERMANUAL 10

9 Removetheshippingbracketthatattaches
thearmtotheinsideoftheunitusingthe
allenwrench(5mm)provided.

Note:Someolderunitshave2cleartubing
piecesthatarewrappedaroundthelead
screwinstead,theseshouldberemovedand
saved.

10 Removethewhitefoamblockpreventingthe
trayfrommoving.

11 Placeallpackagingmaterialsinsidethecrateforlong‐
termstorage.DONOTTROWAWAYtheinterior
cardboardboxesasthesearecustommadeforthe
PPMX.

Keeptheshippingbrace,shipping
bracket,allenwrenchandfoam
blockwiththepackaging.
RemovableTray
Aremovabletrayforplacementoflabwareandtipracksis
required.Whileholdingthehandlesonthesidesofthetray,
lowerthetrayonto
 themetalcarrier.Itiskeyedandwillonly
mountoneway.Observethat
 thetrayislevelandfully
seatedonthemetalcarrier.

RearPanelConnections
Refertothediagramsonthenextpagewhenmakingthe
connectionsdescribedinthissection.
Theinput/outputcontactsontherearpanelareusedfor
communicationbetweenthealignmentheadandTRILUTION
micro
 andforcommunicationbetweentheexternalsafety
interlocksensorandTRILUTIONmicro.Thecontactscanalso
beusedto
 controlperipheraldevices.Refertothediagramfor
thelocationoftheinput/outputports.
ContactInputs–ThebottomterminalblockonthePPMXhas
twopairedinputcontactsthatarelabeled4and5.Never
connectvoltageshigherthan5VDCtoaninput.Whenusing
TTLsignals,besuretomatchGROUNDconnections.

ContactOutputs–ThebottomterminalblockonthePPMX
hastwopaired,isolated‐relaycontactclosuresthatare
labeled1and2.



ASSURANCEGDS®PPMXUSERMANUAL 11

DCPowerOutput–Thetopterminalblockhasone+12VDCoutput,whichislabeled.
ThePPMXhasonefunctionalUSBhostport(themiddleport)ontherearpanel.Thetopportontherearpanelis
not
 supported.TheUSBportscanbeusedtoconnectUSBdrives(whichcanalsobeseenbythetabletwhen
pluggedintothePPMX),
 keyboard,mouse,oranyothercompatibledevice(heater/chiller,barcodescanner,or
shaker,forexample).
TheUSBdeviceportisusedforcommunicationbetweenthePPMXandthetabletorcomputer.AUSBcableis
providedasastandardaccessory.RefertothediagramforthelocationoftheUSBdeviceport.
TheEthernetPort,RS‐232andHDMIarenotsupported.
1 Input/Output(Green)
2 USBhost(NotSupported)
3 USBhost
4 Ethernet(NotSupported)
5 USBdevice
6 RS‐232(NotSupported)
7 HDMI(NotSupported)
8 Powerreceptacle
9 Powerswitch(MAINS)
Usethepowercordontheexternalpower  supplytomaketheconnectionbetweenthe  powerreceptacleonthe
PPMXandthe
 externalpowersupply.Theconnectionfromtheexternalpower  supplytothePPMXusesa
connectorwith
 alockingcollar.Checkthealignmentofthe  pinsandthenpushitinuntilitclicksandlocks  in
place.Todisconnect,pullbackonthe
 lockingcollarandthendisconnectthecable  fromtherearpanelofthe
PPMX.Locatetheappropriatepowercordforyourlinevoltageandthenmaketheconnectionbetweenthe
externalpowersupplyand
 theACpowersource.InstallthePower/USBCablingkitbyfollowingthesupplied
instructions.

ASSURANCEGDS®PPMXUSERMANUAL 12
FrontPanelConnections
ThePPMXhastwofunctionalUSBhostportsonthefrontpanel.TheUSBportscanbeusedtoconnectUSB
drives(whichcanalsobeseenbythetablet/laptopwhenpluggedintothePPMX),
 keyboard,mouse,orany
othercompatibledevice(heater/chiller,barcodescannerorshaker,forexample).

Inasituationwhereanemergencystopisrequired,pressingtheSTOPbuttonstopsthePPMXimmediately.The
protocol
 stopsandresultsaredisplayed(aftertouchingOKontheprompt).

1 USBhost
2 STOP


UPSBatteryPowerandLineConditioner
TopreventfluctuationsinpowertothePPMXandtablet/laptop,itisrecommendedthatthefollowingUPS
batterybackupbeusedwiththesystem:
TrippLiteSU750XL or equivalent

Additionally,itisrecommendedthatthePPMXbeplacedonaseparatecircuitfromotherequipment.

PowerOnandStartUp
TostartthePPMX:

1 MakesurethatthePPMXisconnectedtotheexternalpowersupplyandthattheexternalpowersupplyis
connected
 toapowersource.
2 Turnpoweronattheexternalpowersupply(Oisoff,Iison).
3 TurnthePPMXpoweronusingtheMAINSpowerswitchlocatedontherearpanel.Theindicatorlighton
thefrontpanel
will illuminategreen.
4 Connectthecontroltablet/laptoptothePPMXandpoweriton.
5 Closetherotatingcoverdoor.ThePPMXwillnotoperatewiththecoveropen.

LogIn
Whenprompted,enterthe“Username”and“Password”,andthenselectAccept
(ortheXtocancel).Passwordiscase‐sensitive.Itisrecommendedtochangethe
passwordforthedefaultAdministrator(admin).

1Selectthe“Username(Fullname)”insoftwaretogotothe“User
properties”screen.
2EnteranewPasswordfortheuser,andthenenterthepasswordagaininthe“Passwordconfirm”
field.
3SelectSave,orBacktoreturntothepreviousscreenwithoutsaving.

ThedefaultUsernameis  adminandthePasswordisGilson268


ASSURANCEGDS®PPMXUSERMANUAL 13
UserManagement
TheusermanagementfeatureinTRILUTIONmicroprovidesawayfor
administratorstograntorrestrictusersaccesstoprogramsand
featuresinthesoftware.Usermanagementisaccessiblefromthemain
screenshownwhenthesoftwarestarts.

1SelectManageUsersinsoftwaretogotothe“User
management”screen,whichdisplaysalistofusers.
2Selectauserfromthelist.
3SelectEdit.The“Userproperties”screenappears.

4Editanyorallpropertiesshown.
• “Username”maynotbemodifiedfortheloggedin
user.
• Forthedefaultadministrator(admin),only“Password”
and“Featurepermissions”maybemodified.
5SelectSave,orBacktoreturntothepreviousscreenwithout
saving.

Toaddauser(Administratorsonly),selectAddandthe“Newuser”screenappears.

1 EnteraUserName.
• Theusernamecannotbethesameasanyotherusername.
• Theusernamecanbefrom1to50charactersinlength,mustbeginwithanEnglishletteror
numberandmaycontainthefollowingspecialcharacters:_()‐@.and,.Spacesarevalid.

2 EntertheFullnamefortheuser.
• Thefullnamecanbefrom1to100charactersinlength,mustbeginwithanEnglishletteror
numberandmaycontainthefollowingspecialcharacters:_()‐@.and,.Spacesarevalid.

3 Optionally,entera“Password”fortheuser.Theusercanchangethepasswordatanytime.
• Itcanbefrom1to50charactersinlength,mustbeginwithanEnglishletterornumberandmay
containthefollowingspecialcharacters:_()‐@.and,.Spacesarevalid.
• Itiscase‐sensitive,butitisnotrequired(passwordcanbeblank).
• Ifapasswordwasentered,re‐enterthepasswordinthe“Passwordconfirm”field.

4 Select“Isadministrator”toassignadministratoraccesstotheuser.Bydefault,“Isactive”isselected.Clear
thecheckboxtodenytheuseraccesstothesoftware.Theuserremainsinthelistandcanbechangedto
activebyanAdministratoratanytime.

5 Under“Featurepermissions”,clearthecheckboxforanysoftwaretowhichtheusershouldnothaveaccess.
Bydefault,anewuserhasaccesstoallsoftware.

6 SelectSave,orBacktoreturntothepreviousscreenwithoutsaving.

Todeleteauser(Administratorsonly),selectauserfromthelistandselectDelete.Amessagewillappear
askingifyouwishtodeletetheuser.SelectYestodelete,orNotoclosemessagewithoutdeletingtheuser.




ASSURANCEGDS®PPMXUSERMANUAL 14
TouchscreenOverview
ThefollowinginformationprovidesanexplanationofuniquesoftwareinteractionswhenrunningTRILUTION
microonthe
 touchscreencontroltablet.Itisrecommendedtokeepthetabletpluggedintoapowersource
wheneverpossible.CommunicationerrorscanoccurbetweenthePPMXandcontroltabletifthebatteryruns
toolow.

Refertothetablebelowforadescriptionoftheiconsusedinthe
 on‐screenkeyboard.

ThefollowingiconsappearonseveralscreensinTRILUTIONmicro.
Toviewtheoptionstoshutdownthecontroltabletand/orTRILUTIONmicro,touchonthemainmenu:

• TouchShutdowntocloseTRILUTIONmicroandshutdownthecontroltablet.
• TouchRestartTRILUTIONmicrotorestartonlythesoftware,andnotthecontroltablet.
• TouchCanceltogobacktothemainmenu.


ASSURANCEGDS®PPMXUSERMANUAL 15
AlignmentProtocol
RuntheAlignmentProtocoltoensureproperalignmentofthePPMX.Itisonlynecessarytorunthisprotocol
whensetting
 uptheinstrumentforthefirsttimeafteramoveorifanalignmentissueissuspected.

1 Passtheendofthealignmentcablecordwithpre‐wired
connectorthroughthe
 outletatthebackofthePPMX.Besure
togoundertheleadscrewtoallowforthefullrangeofmotion.

2 TheconnectorislabeledBOTTOMbecauseitconnectstothe
bottomsetof
green input/outputportsontherearpanelof
thePPMX.Maketheconnection.









3 Theclipstosecurethetipracksonthetraycan
interferewiththealignment.Ensure
thattheclips()arepositionedasshownin
thediagrambelow,andthatthe
 thumbscrews
havebeentightened.

4 Closetherotatingcover.

5 ThetabletstartsTRILUTIONmicroautomatically
onpowerup.Ifusingalaptop,doubleclickthe
TRILUTIONmicroicontostartthesoftware.

6 SelectRun/manageprotocols.Ignorethe“Createprotocol”and
“Startutility”bars.

7 SelectRunaprotocol. 

8 SelectAlignmentProtocolandthenselectNext. 

9 TheScanningdialogappearswhilethesoftwarechecksthat the
PPMXis
 connected.Ifconnected,thesoftwaregoestothenext
screen.Ifnotconnected,a
 messagewillappearsuggesting
possiblesolutions.


10 SelectSkipsetuponLabwaresetupguide.

11 SelectRunprotocol. 

12 Waitapproximately3minwhiletheAlignmentProtocolruns.

13 AnotificationappearswhentheAlignmentProtocoliscomplete.


ASSURANCEGDS®PPMXUSERMANUAL 16
14 SelectContinue.

15 SelectHometogotothemainmenu

AddPipetteandPickPenHead(s)
Eachheadthatmaybeusednow,orinthefuture,mustbeaddedpertheinstructionsthatfollow.

1 OntheTRILUTIONmicromainmenu,selectSettings.
2 OntheSettingsmenu,selectPipetteheads.
3 Entertheserialnumberlocatedonthepipetteheadandthepipettetype(i.e.8x200).
4 ThePickPenheadisaddedthesamewayusingserialnumberHN12017.AllPickPenheadsusethissame
serialnumberinthesoftware(theyarephysicallyserializedonthebackfortrackingpurposes).Choose
PickPenIIasthetype.
5 SelectSave.Therewillnotbeamessagethatthesettingsweresaved.
6 Repeatsteps3–5foradditionalheads.Whenfinishedaddingheads,  selectBack.



SetDate&Time
Setthedateandtimetoensureacorrectdate/timestamponprotocol
runs.Thisshould
 onlyhavetobedoneonce.OntheSettingsmenu,select
Date/time,andthensetthedate,time,andtimezone.

InstallChimeSoundBox
ToenablesoundonthePPMX,aseparatechimesoundboxcanbeaddedtothebackofthe
instrumentpertheinstructionsthatfollow:

1 Plugthegreenphoenixconnectorintothetopgreeninput/outputportonPPMXrear
panel.
2 Positionthesoundboxonthebackcenter,abovetheshelfportionof
thePPMXandsecuretheplasticholderusingtheprovidedhardware
(theshorterscrewsareusedonthetopholes).Iftherearealready
screwsinthelocationsspecified,removethesefirst.
3 Adjustthevolumeusingthevolumedial.
4 Thereisalsoaresetbuttonontheinsideofthespeaker.Touse,push
andthenrebootthePPMXsystem.

Volumedial

Resetbutton


ASSURANCEGDS®PPMXUSERMANUAL 17
RunaProtocol
TheLabwaresetupguidescreendisplaystheoptionsforconfirmingtheinstrumentsetup.
SelectStep‐by‐stepwizardtodoallofthefollowing(intheordershown):
• Reviewalistofthematerials.
• Reviewthelayoutofthetrayandsetuppipettehead.
• Setupthepipette,tiprackandadditionallabware.
• Reviewprotocol/deck.
Fordetails,refertoStep‐by‐stepWizardonthenextpage.

SelectBrowsepositionsmanuallytodoanyofthefollowing:
• Reviewthelayoutofthetrayandreviewpipettehead.
• Selectandsetupspecificlabware.
• Reviewprotocol/deck.

SelectSkipsetuptoskipsetupinstructionsandgototheWizardcompletescreen.Protocolwillbesettothe
default
 valuesorthevaluesusedthelasttimetheprotocolwasrun.Thisincludesthenumberofsamplesand
tipsinthereloadblock.

Step‐by‐stepWizard
1 Selecttogotothemainmenu.
2 SelectRunaprotocol.
3 Selectyourprotocol,andthenselectNext.
4 TheScanningdialogappearswhilethesoftwarechecksthatthe
PPMXis
 connected.Ifconnected,thesoftwaregoestothenext
screen.Ifnotconnected,a
 messagewillappearsuggesting
possiblesolutions.
5 Entersamplenumber,kitandreagentinformationmanuallyor
usingabarcodereaderandthenselectNext.
6 SelectStep‐by‐stepwizard.
7 GatherthematerialsintheMaterialsListandthen  selectNext.
8 Waitforthesoftwaretofinish“Preparingpipettehead”andthen
opentherotatingcover.

Steps9–11onlyneedtobecompletedduringinstallationorafter
runningthealignmentprotocol.
9 Removethealignmentheadbydisconnectingtheterminalblock
connectorfromtherear
 panel,andthenremovingthethumbnuts
securingittotheupperpipetteheadassembly.
 
10 Installthepipetteheadontheleftupperpipetteheadassembly
usingthe
 thumbnuts.Ensureproperalignmentofthepipettehead
againsttheupper
 pipetteheadassemblybeforetighteningthe
thumbnuts.
11 Selecttheserialnumberthatmatchestheinstalledpipettehead
fromthedrop‐downlist.
12 InstallthePickPenheadontherightupperpipetteheadassembly
bypushingitontothetwoprongs.Ensureproperalignmentofthe
PickPenheadagainsttheheadassembly,nogapsshouldbevisible.

ASSURANCEGDS®PPMXUSERMANUAL 18
NOTE:ThePickPenheadshouldnotbeusedwhenrunningtheR‐
protocols. 

13 SelecttheserialnumberofinstalledPickPenheadfromthedrop‐
downlist,close
 therotatingcover,andthenselectNext.
14 ProceedthroughtheTipsetupandLabwaresetupscreensbyreviewingthe
information,makinganydesired
 changes,andthenselectingNext.Thescreens
indicatethelabwaretouseandwheretoplaceitonthetray.

Eachtiprackconsistsofatippackandatipreloadblock.
 To
assemblethetiprack:

1 Openthesealedblisterpack.
2 Liftthetippackoutoftheblisterpack.
3 Setthetippackonthetipreloadblock.Ensurethatthe
tippackisfullyseatedon
 thetipreloadblock. 
4 MarktheclearcoverwithanXandremove.
5 Savetheclearplasticboxandcoveronhandforunusedtips.

Toinstallthetiprack: 
1 Loosentheelbowthumbscrewforeachclip.
2 Placethetiprackontheremovabletraywiththebeveled
cornerinthe
 backleftcorner.
3 Rotateeachclipsothatitwilleffectivelysecurethetiprack
onthetrayasshown
 intheimagebelow.
4 Tightentheelbowthumbscrewforeachclip.
5 Pipettetipsareusedfromthebottomrightand
movingupthecolumn.
UsetheTipMovertoloadthePickPentipsfromthetip
racks(thetipmovercanalsobeusedtorefillpipettetip
racks).PickPentipsareusedfromthetopleftand
movingdownthecolumn.

NOTE:AlwaysrefillthepipetteandPickPentipsoffthe
decktopreventtipsfromfallingintotheopeninstrument
tracks.
PlaceSampleBlock(s)withtheBioControllabelontheleftside,stripsarefilledstartinginthetopleftwell
movingdownacolumn.PlacetheResuspensionPlate(s)withtheA1inthetopleftcorner,columnsare
filledstartinginthetopleftwellmovingdownacolumn.RSPlatecolumnsarefilledinaskippedpattern
similartothemanualprocess.Thereservoir(s)shouldalsobeplacedsothattheA1isinthetopleft
corner.


ASSURANCEGDS®PPMXUSERMANUAL 19
PlacetheAssuranceGDSReagentHolderonthedecksothatthesmaller
holeisonthetopleftside
StandardConcentrationReagentbottle(a)
HighThroughput(HT)Reagentbottle(b)
Ifusingthestandardbottle,selectFalse.IfusingtheHTbottle,selectTrue.
ThereareindentationsfortheCRbottlelidsontheholder. 

PlacetheGelCoolingBlocksothatthebrandnameistothe
frontrightside.PlacetheHTAmplificationTubeBlockwiththe
slidebarontheleftside.Slicetheamptubesandexpandthe
platepriortoaddingthelid.


15 IfindividualsampleIDsneedtobeenteredforeachsamplein
thesampleblock,thiscanbedonemanuallyorusingabarcode
reader.

Selectthesamplethatmatchesthelocationonthesampleblock,highlighttheinformationinthefieldand
eithermanuallytypethesampleIDorusethebarcodereadertoimporttheinformationforeachwell.
Thesampleselectedwillbehighlightedonthesampleblock.SamplesarelistedinrowsstartingwithA1.

16 Reviewprotocol/deckandthenselectNext.
17 SelectRunprotocol.
18 Whenprompted,Skipsimulationrequest.










a
b