Biosan PST-60HL User manual

DOMINIQUE DUTSCHER SAS

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DOMINIQUE DUTSCHER SAS

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Contents
1. Safety Precautions ....................................................................................... 4
2. General information ...................................................................................... 6
3. Getting started .............................................................................................. 7
4. Operation ...................................................................................................... 8
5. Calibration .................................................................................................. 10
6. Specifications ............................................................................................. 12
7. Maintenance ............................................................................................... 13
8. Warranty and Claims .................................................................................. 14
9. Declaration of Conformity ........................................................................... 15
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1. Safety Precautions
The following symbols mean:
Caution! Make sure you have fully read and understood the present
Manual before using the equipment. Please pay special
attention to sections marked by this symbol.
Caution! Surfaces can become hot during use.
GENERAL SAFETY
Use only as specified in the Operating Manual provided.
Save the unit from shocks or falling.
Store and transport the unit in a horizontal position (see package label).
After transportation or storage and before connecting to electric circuit, keep
the unit under room temperature for 2-3 hrs.
Before using any cleaning or decontamination methods except those
recommended by the manufacturer, check with the manufacturer that the
proposed method will not damage the equipment.
Do not make modifications to the design of the unit.
ELECTRICAL SAFETY
Connect only to the external power supply unit with voltage corresponding to
that on the serial number label.
Use only the external power supply unit provided with this product.
Ensure that the switch and external power supply are easily accessible during
use.
Do not plug the unit into an ungrounded power socket, and do not use an
ungrounded extension lead.
Disconnect the unit from electric circuit before moving. Switch the unit off and
disconnect the power cord plug from power socket to disconnect the unit from
electric circuit.
hazardous material is spilt on or penetrates into the equipment. If liquid
penetrates into the unit, disconnect it from electric circuit and have it checked
by a repair and maintenance technician.
Do not operate the unit in premises where condensation can form. Operating
conditions of the unit are defined in the Specifications section.
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DURING OPERATION
Do not leave the operating unit unattended.
Do not impede the platform motion.
Do not operate the unit in environments with aggressive or explosive chemical
mixtures. Please contact manufacturer for possible operation of the unit in
specific atmospheres.
Do not operate the unit if it is faulty or has been installed incorrectly.
Do not fill in the microplates after they have been inserted in the unit.
Do not use outside laboratory rooms.
Do not check the temperature by touch. Use a thermometer.
BIOLOGICAL SAFETY
hazardous material is spilled on or penetrates into the equipment.
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2. General information
PST-60HL / PST-60HL-4 Plate Shaker-Thermostat is designed for shaking 1
to 4 standard 96-well plates in the thermal regulation mode.
Plate Shaker-Thermostat was designed using the multi-system principle,
which allows using it as three independent devices:
1) incubator for lasting incubation of micro quantities (insect, plant cell cultures,
etc.) in plates;
2) plate shaker for operation in the cold room or other conditions, which do not
require temperature stabilization;
3) microplate thermo-Shaker for immunochemistry and molecular diagnostics,
where the requirements to the result reproducibility and thus to the precise
method regulation are particularly high.
A distinctive feature of Biosan plate thermoshakers is the patented two-side
plate heating that allows to achieve full correspondence of the set and actual
temperature in the plate wells.
PST-60HL/PST-60HL-4 Plate Shaker-Thermostat provides:
soft or intensive sample shaking;
rotation speed regulation, stabilization and indication;
even shaking amplitude throughout Shaker-Thermostat platform;
required operation time setting and indication;
automatic stopping of the platform movement after the set time expires;
current operation time indication;
setting and indication of the required temperature;
fault automatic diagnostics (temperature sensor, platform heating, lid heating
and other errors).
The device can be used in:
cytochemistry
for in situ reactions;
immunochemistry
for immunofermentative reactions;
biochemistry
for enzyme and protein analysis;
molecular biology
for matrix analysis, DNA and RNA analyses.
The maximum guaranteed number of diagnostic cycles in the Plate Shaker-
Thermostat mode, which require 15-30 min work in one cycle, is 7000-14000 times.
External 12V power supply unit is used to power the device. It makes it safe
to work in the cold room, where condensation may cause leakage current from
electric circuit.
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3. Getting started
3.1. Unpacking.
Remove packing materials carefully and retain for them future shipment or
storage of the unit.
Examine the unit carefully for any damage incurred during transit. The
warranty does not cover in-transit damage.
Warranty covers only the units transported in the original package.
3.2. Complete set. Package contents:
- PST-60HL / PST-60HL-4 Plate Shaker-Thermostat .............................. 1 pce
- spare rubber belt ...................................................................................2 pcs
- external power supply ............................................................................ 1 pce
- power cord ............................................................................................. 1 pce
- Operating manual, Certificate ..............................................................1 copy
3.3. Set up:
- place the unit upon even horizontal non-flammable surface away from any
flammable materials (not less than 30 cm);
- remove protective film from the display;
- plug the external power supply unit into the socket at the rear side of the unit
and position the unit so that there is easy access to the power switch and
the external power supply unit.
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4. Operation
Recommendations during operation
For proper mixing, it is not recommended to fill microplates for more than 75%
of their nominal volume.
Seal the microplates with an appropriate adhesive film to avoid spilling the
sample.
Fig.1 Control panel
4.1. Connect the external power supply unit to a grounded power socket and set
the power switch located on the rear panel of the unit to position I (ON).
4.2. The display will turn on. Upper line (Set) shows time, speed and temperature
set earlier. Lower line (Actual) shows current readings of the same
according to the temperature set in the upper line). The time of temperature
stabilisation depends on the initial temperature.
4.3. Setting the parameters. Use the readings in the upper line of the display (Set),
while setting the necessary parameters. Pressing the key for more than 3 s
will increase the increment.
4.3.1. Setting time (TIME) he required
working time interval in hours and minutes (increment 1 min).
4.3.2. Setting speed (RPM). uired
speed (increment 10 RPM).
4.3.3.
Caution! The platform heating can be turned off only by setting the
- - set point).It can be used in cold rooms as a mixing
device without thermal regulation in this mode.
4.4. Program execution. After the thermal stabilisation of the unit (when the set
and current temperature readings become the same):
4.4.1. Microplate fixation:
PST-60HL: Place microplates on the platform and fix it with the special
push-down clip by pressing it against the plate covers.
PST-60HL-4: Unscrew the fixation screws. Place microplates on the
platform and fix them by tightening the fixation screws.
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Caution! For model PST-60HL: Load only pairs of microplates for best
fixation.
Correct loading
Wrong loading
Caution! Do not fill in the microplates after they have been inserted in
the unit.
Caution! For model PST-60HL-4: The microplate fixation screws must
always be tightened to avoid damage. Tighten the fixation
screws completely when microplates are removed from or
placed on the platform. Do not close the lid if the microplate
fixation screws are not tightened to avoid damage.
4.4.2. Press the RPM-RUN/STOP key (Fig. 1/4). The platform will start rotating and
the timer indicator will start counting up the time interval (with 1 min precision).
4.4.3. After finishing the program, the platform motion will stop and the timer will
show the flashing reading STOP accompanied by the repetitive sound signal
until the RPM-RUN/STOP key is pressed.
4.5. If the working time is not set (or is reset) and the timer indicator in the upper line
shows 00:00, pressing the RPM-RUN/STOP key will start continuous operation
of the Thermo-shaker (timer indicator will start counting up the time interval in
the lower line (Actual)) until the RPM-RUN/STOP key is pressed again.
Please note! The platform temperature will be constantly maintained in
accordance with the set temperature. This allows using the
device again without pre-heating.
4.6. The timer can be reset during operation if required. Press the TIME-
RUN/STOP key once (Fig. 1/5) to stop the timer. Press the TIME-RUN/STOP
key again to restart the timer.
4.7. The platform motion can be stopped at any time by pressing the RPM-RUN
/STOP key. In this case the program realisation will stop and the timer will
switch into the STOP mode saving previously set time. Press the RPM-
RUN/STOP key to repeat the operation with the same time and speed.
Caution! At the end of the set time period the platform movement is
stopped automatically, but the heating can be stopped only by
, lower
button) till the OFF sign appears in the upper part of the display.
4.8. After finishing the operation set the power switch, located on the rear panel of
the unit, in position O (Off) and disconnect the external power supply from
electric circuit.
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5. Calibration
Attention! Calibration is available for models of version V.6AW (PST-
60HL) and V.4AW (PST-60HL-4).
5.1. The device is pre-calibrated at the factory (calibrating coefficient is 1.000) for
operation with temperatures, measured by a sensor, installed in the heating
block.
5.2. To enter the calibration coefficient, hold the Stop key (fig. 1/8) pressed for
more than 8 s to activate calibration mode. The calibration coefficient will be
shown on the display (fig. 2/1).
5.3. Restoring factory settings. Temp keys
(fig. 1/6) as shown on Fig. 2/1 to restore the factory settings. Press the Run
key (fig. 1/7) once to save the changes and exit the calibration mode.
Note. Coefficient value changes are recommended after the unit has
5.4.1. Calibration procedure.
spectrocells, placed into the block sockets.
5.4.2.
Fig.2 Control panel in calibration mode
5.4.3. After the unit reaches the set temperature (when the set and current
temperature readings equal) leave the unit for 30 minutes for thermal
stabilization.
5.4.4.
s actual temperature is 40
correction.
5.4.5. Hold Stop key (fig. 1/8) pressed for more than 8 s to activate calibration mode.
The following parameters will be shown on the display (fig. 2).
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5.4.6. Temp keys (fig. 1/6), change the calibration coefficient
(fig. 3/1) so that the new temperature value (fig. 3/2) corresponds to the
independent sensor temperature. In our example, the calibration coefficient
will be 0.974.
Note. Calibration coefficient can be changed in range from 0.968 up
to 1.031 with increment 0.001. This calibrating coefficient will
correct temperature through all the operation range.
Note. Coefficient value changes are recommended after the unit has
5.4.7. After finishing the calibration press the Run key (fig. 1/7) once to save the
changes and exit the calibration mode.
5.4.8. The display will show calibrated temperature as shown on fig. 4/1 and the unit
will continue thermal stabilization according to the previously set temperature.
Fig. 3 Control panel in calibration and
operation mode
Fig. 4 Control panel in operation mode
after calibration
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6. Specifications
The unit is designed for operation in cold rooms, incubators and closed
-condensing
decreasing linearly to 50% relat
6.1. Temperature specifications
Setting range ........................................................................
Control range ......................................................
Setting resolution ..................................................................................
Stability ................................................................................................
Accuracy ..............................................................................................
...........................................................................
....................................... 12 min
Temperature calibration option*
Calibration coefficient range* ......................................
6.2. General specifications
Speed setting range .............................................................. 250 - 1200 rpm
Speed setting resolution ..................................................................... 10 rpm
Orbit ....................................................................................................... 2 mm
Display .................................................................................. 16x2 signs, LCD
Digital time setting range ........................................ 1 min 96 hrs / non-stop
Max. continuous operation time ....................................................... 96 hours
(recommended interval between operation sessions not less than 8 hours)
Time setting resolution .......................................................................... 1 min
Max. height of microtest plate ............................................................. 18 mm
External power supply .......... input AC 100-240 V 50/60 Hz, output DC 12 V
PST
-
60HL
PST
-
60HL
-
4
Current/power consumption
12 V DC, 3.3 A / 40 W
12 V DC, 4.15 A / 50 W
Number of microplates
2
4
Platform dimensions
250 x 150 mm
210 x 290 mm
Dimensions
270x260x125 mm
380x390x140 mm
Weight
**
6.1 kg
8.8 kg
Replacement parts
Description
Catalogue number
Rubber belt
122x0.6x6 mm
BS
-
000000
-
S18
Biosan is committed to a continuous program of improvement and reserves
the right to alter design and specifications of the equipment without additional notice.
* Only for models of version V.6AW (PST-60HL) and V.4AW (PST-60HL-4).
**
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7. Maintenance
7.1. If the unit requires maintenance, disconnect the unit from the mains and
contact Biosan or your local Biosan representative.
7.2. All maintenance and repair operations must be performed only by qualified
and specially trained personnel.
7.3. Standard ethanol (75%) or other cleaning agents recommended for cleaning
of laboratory equipment can be used for cleaning and decontamination of the
unit.
7.4. Rubber belt replacement.
For maintenance of reliable operation of the device, the producer recom-
mends to replace rubber belts after 1.5 years or 2000 hours of operation time.
To replace the belt:
- Disconnect the external power supply unit
from the device.
- Remove 4 fixation screws on the underside
and remove the bottom plate.
- Replace the rubber belt (fig. 5).
- Reassemble the device.
Fig. 5. Rubber belt
replacement
7.5. Error codes in case of a defect.
Following error codes are shown in the lower right corner of the display (fig. 1),
accompanied by a sound signal every 8 s. Press RPM RUN/STOP key to turn
off the signal. Disconnect the unit from the electric circuit and contact Biosan
or your local Biosan representative.
Error code
Description
ERR 1
Lower
plate heat sensor error
ERR 2
Upper lid heat sensor error
ERR 3
PCB plate error
ERR 4
PCB plate error
ERR 5
Upper lid heating error
ERR 6
Upper lid overheating
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8. Warranty and Claims
8.1. The Manufacturer guarantees the compliance of unit with the requirements of
Specifications, provided the Customer follows the operation, storage and
transportation instructions.
8.2. The warranted service life of unit from date of delivery to the Customer is 24
months (excluding consumables such as rubber belts). Contact your local
distributor to check availability of extended warranty.
8.3. Warranty covers only the units transported in the original package.
8.4. If any manufacturing defects are discovered by the Customer, an
unsatisfactory equipment claim shall be compiled, certified and sent to the
local distributor address. Please visit www.biosan.lv, Technical support
section to obtain the claim form.
8.5. The following information will be required in the event that warranty or post-
warranty service comes necessary. Complete the table below and retain for
your records.
Model PST-60HL / PST-60HL-4 Plate Shaker-Thermostat
Serial number
Date of sale
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9. Declaration of Conformity
Edition 4.-6.01 April 2016
DOMINIQUE DUTSCHER SAS

DOMINIQUE DUTSCHER SAS