Bruker bravo User manual
BRAVO
Handheld Raman Spectrometer
User Manual
1837209
Draft version
1st edition 2015, publication date May 2015
© 2015 BRUKER OPTIK GmbH, Rudolf-Plank-Straße 27
D-76275 Ettlingen, www.bruker.com/optics
All rights reserved. No part of this manual may be reproduced or transmitted in any form
or by any means including printing, photocopying, microfilm, electronic systems etc. wit-
hout our prior written permission. Brand names, registered trademarks etc. used in this
manual, even if not explicitly marked as such, are not to be considered unprotected by
trademarks law. They are the property of their respective owner.
This manual is the original documentation for the handheld Raman spectrometer
BRAVO.
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Table of contents
Table of contents
1 Safety..............................................................................................................5
1.1 General safety information.................................................................................... 5
1.2 Classification of the safety notes ......................................................................... 5
1.3 Overview of possible types of hazard.................................................................. 6
1.3.1 Possible hazards during installation and operation.................................................. 6
1.3.2 Possible hazardous sample materials...................................................................... 7
1.4 Laser safety ............................................................................................................ 8
1.4.1 Laser class...............................................................................................................8
1.4.2 Safety instructions.................................................................................................... 8
1.4.3 Laser exit points....................................................................................................... 9
1.4.4 Laser status LED.................................................................................................... 10
1.4.5 Enabling / disabling the laser ................................................................................. 11
1.4.5.1 How to enable the laser ......................................................................................... 11
1.4.5.2 How to disable the laser ........................................................................................ 11
1.4.5.3 How to abort a running measurement.................................................................... 12
1.5 Fire safety ............................................................................................................. 12
1.6 Battery safety instructions.................................................................................. 13
1.7 Safety notes regarding the LCD.......................................................................... 13
1.8 Electromagnetic compatibility (EMC)................................................................. 14
1.9 Intended use ......................................................................................................... 14
1.10 Service contact data ............................................................................................ 15
2 General information ....................................................................................17
2.1 Fields of application............................................................................................. 17
2.2 Technical feature.................................................................................................. 17
2.3 Scope of delivery.................................................................................................. 18
2.4 Inspecting the packaging.................................................................................... 19
2.5 Ambient conditions and power supply .............................................................. 19
3 BRAVO analyzer - Overview.......................................................................21
3.1 Components and control elements - Overview................................................. 21
3.2 Laser status LED ................................................................................................. 22
3.3 Measuring tip........................................................................................................ 23
3.4 Button panel - Control buttons ........................................................................... 24
3.5 Touchscreen......................................................................................................... 24
3.6 Docking station .................................................................................................... 25
4 Operation......................................................................................................27
4.1 Switching on / off BRAVO .................................................................................. 27
4.2 Logging in............................................................................................................. 28
4.3 Scanning the barcode of the sample.................................................................. 29
4.4 Exchanging the measuring tip............................................................................ 30
4.5 Verifying a sample ............................................................................................... 31
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4.6 Aborting a running measurement.......................................................................33
4.7 Exchanging the battery........................................................................................34
4.8 Recharging the battery ........................................................................................35
4.8.1 General information................................................................................................35
4.8.2 Recharging the battery with the battery charger.....................................................36
4.8.3 Recharging the battery via the docking station.......................................................37
4.9 Establishing a wireless connection between BRAVO and a data station.......38
4.9.1 Connecting the data station to the WLAN ..............................................................38
4.9.2 Connecting BRAVO to the WLAN ..........................................................................39
4.9.3 Connecting the data station to BRAVO via WLAN.................................................41
4.10 Establishing a wired connection between BRAVO and a data station............42
4.10.1 General information................................................................................................42
4.10.2 Possible connection variants..................................................................................42
4.10.3 Assigning network addresses to BRAVO...............................................................45
4.10.4 Connecting BRAVO to data station .......................................................................47
4.10.5 Checking the connection between BRAVO and data station .................................47
4.11 Mounting / demounting the carrying strap ........................................................48
4.11.1 General information................................................................................................48
4.11.2 Procedure...............................................................................................................48
5 Cleaning and Decontamination..................................................................51
5.1 Cleaning the BRAVO analyzer.............................................................................51
5.1.1 Cleaning the plastic cover ......................................................................................51
5.1.2 Cleaning the touchscreen.......................................................................................51
5.2 Decontaminating the BRAVO analyzer...............................................................52
5.2.1 Approved decontaminating agents.........................................................................52
5.2.2 Decontamination procedure ...................................................................................52
6 Repair ...........................................................................................................53
6.1 General information .............................................................................................53
6.2 Procedure..............................................................................................................53
7 Troubleshooting..........................................................................................55
7.1 General information .............................................................................................55
7.2 Problem - possible cause - solution...................................................................55
7.2.1 BRAVO does not boot up.......................................................................................55
7.2.2 Log in attempt failed...............................................................................................56
7.2.3 Barcode cannot be scanned...................................................................................56
7.2.4 Black touchscreen display......................................................................................57
7.2.5 Failed PQ test / OQ test .........................................................................................57
7.2.6 Aborted measurement............................................................................................57
7.2.7 Problems regarding the detected spectral signal ...................................................58
7.2.8 Problems regarding wireless data transfer.............................................................58
7.2.9 Problems regarding the docking station.................................................................59
A Specification................................................................................................61
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A.1 BRAVO ................................................................................................................. 61
A.2 Environmental conditions ................................................................................... 61
A.3 Battery and battery charger ................................................................................ 62
A.3.1 Battery ...................................................................................................................62
A.3.2 Battery charger ...................................................................................................... 62
A.4 Docking Station ................................................................................................... 62
A.5 WiFi module.......................................................................................................... 63
A.6 Barcode reader..................................................................................................... 63
A.6.1 Depth of field range ............................................................................................... 63
A.6.2 Resolution ............................................................................................................. 63
B Orderable items ...........................................................................................65
B.1 Spare parts & replacement parts........................................................................ 65
B.2 Reference standards and certified samples...................................................... 65
B.3 Consumables........................................................................................................ 66
B.4 Cleaning utensils.................................................................................................. 66
B.5 Optional parts....................................................................................................... 66
B.6 Network equipment.............................................................................................. 67
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1 Safety
1.1 General safety information
1.2 Classification of the safety notes
Depending on the degree of hazard, important safety notes are classified in this manual
by signal words as follows:
➣Read carefully all instructions and safety notes in this manual before putting the
handheld Raman spectrometer BRAVO into operation. Keep the manual for future
reference available at any time at a suitable place.
➣Always observe the instructions and safety notes given in this manual. Failure to
do so can lead to severe personal injuries and/or property damage. Non-obser-
vance of the instructions and safety notes also violates the intended use of the
handheld Raman spectrometer BRAVO. (See section 1.9.)
➣It is the operator's duty to plan and implement all necessary safety measures and
to supervise their observance. Moreover, the operator must ensure that the hand-
held Raman spectrometer BRAVO is in proper condition and fully functioning.
➣A safe and trouble-free operation of the handheld Raman spectrometer BRAVO is
ensured only if it is operated, maintained and repaired according to the proce-
dures described in this manual and in compliance with all relevant safety stan-
dards and regulation.
➣The handheld Raman spectrometer BRAVO should be operated only by autho-
rized personnel which is trained in operating the instrument and which is familiar
with the relevant safety instructions and laser safety regulations.
➣Do never remove or deactivate any supporting safety systems during the opera-
tion.
The handheld Raman spectrometer BRAVO has been developed according to the
safety regulation EN 61010-1:2010 (IEC 61010-1:2010+Cor.:2011) for electrical
equipment for measurement, control and laboratory use.
DANGER
➣Indicates a hazardous situation which, if not avoided, will result in death or serious
(possibly irreversible) injury and major property damage.
WARNING
➣Indicates a hazardous situation which, if not avoided, could result in death or seri-
ous (possibly irreversible) injury and major property damage.
CAUTION
➣Indicates a hazardous situation which, if not avoided, may result in minor or mod-
erate (reversible) injury and minor property damage.
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iThe symbol iwill draw your attention to additional pieces of information which might be
useful for you.
1.3 Overview of possible types of hazard
1.3.1 Possible hazards during installation and operation
Hazards that can possibly occur during operating the analysis instrument BRAVO are
indicated by the appropriate warning symbols. The following warning symbols indicate
different dangerous situations which may be caused by an improper use of the analysis
system:
➣Important note: All warning labels on the analysis system BRAVO must always be
kept legible. Immediately replace a worn or damaged label.
NOTICE
➣Indicates a practice which could result in property damage if the given instructions
are not observed.
Warning
symbol Definition
General hazard:
This warning symbol indicates general hazard. The label is located
near the danger spot in question. Observe the safety instructions and
follow the precautions described in the manual to avoid personal injury
and/or property damage.
Laser radiation:
This warning symbol indicates the existence of laser radiation. The
label is located near the opening at which hazardous laser radiation
exits the instrument. Do not look directly into the laser beam or use
any kind of optical instruments to look into the beam as this may cause
permanent eye damage.
Electrical shock:
This warning symbol indicates electrical hazard. It is located near live
parts or enclosures behind which are live parts that represent an acci-
dental contact hazard. Do not touch these parts. Before removing the
corresponding housing part and beginning any repair works, first turn
off the mains power switch and unplug the power cord. Prevent all live
parts from coming into contact with a conductive substance or liquid.
Non-observance of the relevant safety instructions can cause severe
injury and/or property damage.
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1.3.2 Possible hazardous sample materials
There can also be hazards caused by the sample material. Depending on the type of
hazardous substances you analyze, you have to observe specific substance-relevant
safety instructions. Affix the corresponding warning label on the appropriate BRAVO
position. The label must be well legible and permanently discernible. The following list
contains some examples of hazardous substances:
➣Important note: In case you have analyzed substances which are dangerous to
health you have to decontaminate the BRAVO analyzer completely before you
return it to Bruker for repair purposes. In addition, you have to add a declaration with
which you confirm that the BRAVO analyzer is completely free of any hazardous
substances. See chapter 6.
Waste disposal
Dispose all waste produced (chemicals, infectious and radioactively contaminated sub-
stances etc.) according to the prevailing laboratory regulations. Detergents and cleaning
agents must be disposed according to the special waste regulations.
Symbol Definition
Infectious material
This warning symbol indicates the possible existence of biologically
dangerous and infectious material. When working with this kind of
material always observe the prevailing laboratory safety regulations
and take necessary precautions and disinfection measures (e.g. wear-
ing protective clothing, masks, gloves etc.). Non-observance may
cause severe personal injury or even death.
For information on how to use, dilute and efficiently apply disinfectants,
refer to the Laboratory Biosafety Manual: 2004 by WHO - World Health
Organization.
Radioactive material
This warning symbol indicates the possible existence of radioactivity.
When working with radioactive material always observe the safety reg-
ulations and take necessary protective measures. Wear protective
clothing, e.g. masks and gloves. Non-observance may cause severe
personal injury or even death.
Corrosive substances
This warning symbol indicates the possible existence of corrosive sub-
stances. When working with corrosive substances always observe the
laboratory safety regulations, and take protective measures (e.g. wear
protective masks and gloves). Non-observance may cause severe per-
sonal injury or even death.
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1.4 Laser safety
1.4.1 Laser class
The handheld Raman spectrometer BRAVO is classified as a laser class 1M product
according to IEC/EN 60825-1:2007. It emits invisible laser radiation in the wavelength
range 700nm to 1100nm (Popt. < 100nm).
Laser class 1M product means that the product is safe for all reasonable foreseeable
conditions of use. This means, no laser safety equipment is required for operating
BRAVO.
The duration of the laser radiation emission is limited automatically to the duration of the
spectroscopic measurement.
☞For information about the laser exit points, see section 1.4.3.
1.4.2 Safety instructions
Although BRAVO is a laser class 1M product, circumstances may occur in which laser
radiation poses a potential health hazard. In these cases, observe the following safety
instructions:
☞For information about how to switch off BRAVO, see section 4.1. For information
about how to remove the battery, see section 4.7. For the Bruker service contact
data, see section 1.10.
This laser warning label identifies BRAVO as a
laser class 1M product. It is affixed at the
bottom side of the handheld Raman
spectrometer.
WARNING
Health hazard because of exposure to laser radiation
Non-observance of the following safety instructions can result in serious injury (possi-
bly irreversible eye injuries).
➣Do not place magnifying optics (e.g. magnifying glass) into the exiting laser beam
which focuses the laser beam. A focussed laser class 1M beam is capable of
causing eye injuries. Note: Means used for the correction of vision defects like eye
glasses or contact lenses do not pose an injury risk to the human eye in this con-
text.
➣In case the housing is damaged (e.g. parts of the housing are broken away) do
not start a measurement. Otherwise, there is a potential risk of laser radiation
emission. Instead, switch off the BRAVO spectrometer immediately and remove
the battery. Contact the Bruker service.
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1.4.3 Laser exit points
The location of the laser exit point depends on the type of installed measuring tip. Only
as long as a spectroscopic measurement is in progress, invisible laser radiation of laser
class 1M exits the handheld Raman spectrometer at the following exit points:
In case of an installed measuring tip
for bags, directed laser radiation
exits BRAVO.
In case of an installed measuring tip
for vials, diffusely scattered laser
radiation exits BRAVO.
Important note: If there is not any
measuring tip installed, the user can-
not start a measurement neither by
pressing the Start Measurement but-
ton at the BRAVO spectrometer
nor by touching the Collect Data icon
on the touch screen. This safety
feature prevents an accidental laser
emission in case no measuring tip is
installed.
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1.4.4 Laser status LED
The laser LED informs you about the current
laser status as follows:
•Laser LED is off: Laser is disabled (i.e. it is
not ready for emitting radiation).
•Blue solid: Laser is enabled (i.e. it is ready
for emitting radiation) and the laser safety
status of the instrument is OK.
•Red solid: Laser emits radiation (for mea-
surement purposes). As soon as the mea-
surement is finished, the laser LED will
return to blue (solid).
•Blue flashing: This indicates a laser safety
problem. In this case, you can not start a
measurement. Contact the Bruker service.
See section 1.10.
Laser status LED
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1.4.5 Enabling / disabling the laser
In the enabled state, the laser is switched on and ready to emit radiation, but it does not
emit radiation. The laser does not emit radiation before the user actively starts a mea-
surement by pressing the button at the BRAVO spectrometer or tapping the icon
on the touchscreen display. The duration of the laser radiation emission is automatically
limited to the measurement time. In the disabled state, the laser is switched off. In this
case, a spectroscopic measurement cannot be performed.
1.4.5.1 How to enable the laser
1.4.5.2 How to disable the laser
Enabling the laser
1. Switch on BRAVO by pressing the ON /
OFF button .
➣After you have switched on BRAVO, the
laser status LED is still off. This indicates
that the laser is not yet activated.
2. Enable the laser by pressing the button
at the BRAVO button panel.
➣The laser status LED lights blue. This
indicates that the laser is ready for emit-
ting radiation and laser safety status is
OK.
i Before starting a measurement, make sure
that the laser is enabled. When you start a
measurement with a disabled laser,
BRAVO aborts the measurement and
delivers a result of the category Aborted.
The reason stated for the abortion is Laser
not enabled.
Disabling the laser (e.g. in case of emer-
gency)
Press the button at the BRAVO button
panel.
➣This action switches off the laser i.e. it is
not ready for emitting radiation any
more. As a consequence, the laser sta-
tus LED turns off.
Note: To re-enable the laser, press again the
button at the BRAVO button panel.
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1.4.5.3 How to abort a running measurement
➣Note: BRAVO emits laser radiation only for the duration of the spectroscopic
measurement (data collection).
1.5 Fire safety
For analyzing purposes, the sample is exposed to laser radiation. This laser radiation
has the capacity to ignite highly flammable materials. So especially in case of highly
flammable substances, there is a potential fire hazard.
Either Press to Abort on the touch screen display...
... or disable the laser by pressing the button at the
BRAVO button panel.
WARNING
Fire hazard when analyzing a highly flammable substance
Non-observance of the following safety instruction could result in serious injury and/
or property damage.
➣Do not analyze knowingly a highly flammable substance.
➣When analyzing unknown material, be aware of the potential fire hazard and take
appropriate precautions (e.g. a fire extinguisher within reach).
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1.6 Battery safety instructions
1.7 Safety notes regarding the LCD
The liquid crystal in the LCD is poisonous. During normal operation, it does not pose any
health hazard. However, should the touchscreen display break and liquid crystal leaks,
be careful.
WARNING
Health hazard because of an exploding battery
Non-observance of the following safety instructions can result in serious injury and /
or property damage.
➣Do not expose the battery to fire, sparks or glowing embers. Do not dispose the
battery in fire. Otherwise, it will explode. Risk of explosion and fire!
NOTICE
➣Avoid excessive physical shock or vibration.
➣Do not short-circuit the battery terminals.
➣Do not disassemble or deform the battery.
➣Do not use a battery which has visible signs of damage.
➣Do not immerse the battery in water or other liquids. Do not allow the battery to
get wet.
CAUTION
Health hazard because of improper handling of leaking liquid crystal
Non-observance of the following safety instructions can result in minor injury.
➣Avoid any skin contact. If you your skin or clothes have come in contact with liquid
crystal, wash it off immediately using soap and water.
➣Do not put liquid crystal in your moth.
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1.8 Electromagnetic compatibility (EMC)
EU regulations
The BRAVO analyzer has been developed according to EN 61326-1:2013 Electrical
equipment for measurement, control and laboratory use - EMC requirements - Part 1:
General requirements. The BRAVO analyzer is a class A equipment.
Class A equipment is suitable for the use in all establishments other than domestic and
those directly connected to a low voltage power supply network which supplies buildings
used for domestic purposes.
➣Important note: In a domestic environment, BRAVO may cause radio interference.
In this case, the operator may be required to take corrective actions in order to stop
radio interference.
US / FCC regulation
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide rea-
sonable protection against harmful interference when the equipment is operated in a
commercial environment.
➣Important note: This equipment generates, uses, and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual, may
cause interference to radio communications. Operation of this equipment in a resi-
dential area is likely to cause harmful interference in which case the user will be
required to correct the interference at his own expense.
Changes or modifications not expressly approved by the party responsible for compli-
ance could void the user's authority to operate the equipment.
1.9 Intended use
BRAVO is a handheld spectrometer intended for Raman spectroscopic measurements
and subsequent qualitative evaluation for the purpose of raw material verification.
Due to the measurement technique, BRAVO is suited for analyzing all kinds of solid and
liquid samples which are Raman active, excluding highly flammable substances for
safety reasons.
BRAVO is intended for indoor usage under the ambient conditions specified in
appendix A.2.
The intended use includes also the compliance with the relevant standards and regula-
tions, especially:
• EN 60825-1 - Safety of laser products - Part 1: Equipment classification and
requirement
• national and local safety regulations
• national and local accident prevention regulations
• generally recognized technical regulations
The intended use also includes the strict observance of all instructions given in this man-
ual, namely:
• safety instructions
• operation and handling instructions
• cleaning instructions
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1.10 Service contact data
In case you have questions about safety, operation and / repair of the BRAVO analyzer
or you need technical assistance in case of a hardware and / or software problem, you
can contact the Bruker service as follows:
• Service hotline hardware: +49 (0) 72 43 504-2020
• Service hotline software: +49 (0) 7243 504-2030
• Fax: +49 (0) 72 43 504-2100
• E-mail: Service.BOPT.DE.mailing@bruker.com
☞On our website www.bruker.com/about-us/offices/offices/bruker-optics you will
find also the current contact data of all Bruker Optics services offices worldwide.
WARNING
Health hazard because BRAVO is used other than for its intended
purpose
Non-observance of the following safety instruction could result in serious injury (pos-
sibly irreversible skin and/or eye injuries).
➣Use BRAVO only as intended. Do not take any action that violates the intended
use. The operational safety BRAVO is ensured only if it is used as intended.
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2 General information
2.1 Fields of application
BRAVO is a handheld Raman spectrometer designed for raw material verification and
raw material identification. It is approved for indoor usage only, namely in laboratory and
warehouse environments. A typical field of application is the incoming goods inspection.
There are two different types of measuring tips which are interchangeable. These mea-
suring tips can be used for measuring liquid and solid samples being filled in a vial or in
a plastic bag, in a barrel, for example. With BRAVO, the samples are measured in a non-
contact way.
2.2 Technical feature
The BRAVO analyzer is characterized by the following technical features:
Duo LaserTM excitation
The BRAVO analyzer is equipped with two laser diodes which emit radiation in the wave-
length range between 700nm and 1100nm. This dual laser excitation provides for a very
high sensitivity across the entire spectral range. This in turn ensures a very high degree
of unambiguous sample identification.
Fluorescence mitigation by sequentially shifted excitation (SSETM)1
Normally, the Raman spectroscopic measurement technique is not suitable for samples
which tend to fluoresce when being exposed to laser radiation because sample fluores-
cence can yield a much more intense signal than the Raman scatter of the sample,
masking any Raman bands in the spectrum.
The patented SSETM technique, however, allows for obtaining pure Raman spectra even
in the presence of intense fluorescence by exciting the sample with a sequentially
shifted laser radiation. Due to the implemented fluorescence mitigation technique
SSETM, BRAVO can also be used for measuring samples which tend to fluoresce when
being exposed to laser radiation.
Inbuilt wavenumber calibration
The automated wavenumber calibration ensures highly reproducible measurement
results and an unmatched wavenumber accuracy. They are crucial for a raw material
identification in a validated environment.
Automatic recognition of the measuring tip
BRAVO recognizes automatically the currently installed type of measuring tip. In addi-
tion, this information is stored automatically in the measurement records.
1. US patent 8,570,507, other patents pending
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General information 2
In case, the user has explicitly defined a certain type of measuring tip for a certain sam-
ple when setting up a library, than BRAVO automatically prompts the user to use the pre-
viously defined tip for measuring the sample in question. This special feature prevents
the user from measuring a certain sample using the wrong measuring tip.
The design of the measuring tip allows for an easy interchange and ensures always a
precise positioning.
Wireless and wired data transmission
BRAVO is equipped by default with an integrated WLAN device. This technical feature
allows for a wireless data exchange between the BRAVO analyzer and a PC station via
a wireless network. If demanded, the wireless connectivity can be deactivated. Alterna-
tively, a wired data transmission via an Ethernet network is also possible by using the
optional docking station.
2.3 Scope of delivery
The items of the default delivery scope are delivered in a robust carrying case.
Standard Components: The carrying case includes the following items:
• handheld Raman spectrometer BRAVO with installed
BRAVO software
• 1x measuring tip for vials
• 1x measuring tip for bags
• 2x rechargeable batteries (Note: The rechargeable
batteries are delivered in a charged state.)
• 1x battery charger
• 1x polystyrene standard (reference standard for OQ
and PQ test measurements)
• 1x calcite standard (reference standard for OQ test
measurement)
• 1x microfibre cloth
• 1x carrying strap (including the required items and
tools for mounting / demounting the strap)
• OPUS software delivered on an USB stick (including
OPUS online help) Note: By default, the following
OPUS software packages are delivered: OPUS/IR,
OPUS/BRAVO, OPUS/SEARCH and OPUS/VALIDA-
TION.
• Quick Start Guide (printed version)
• BRAVO Analyzer User manual and BRAVO Software
User Manual (electronic version, delivered on an USB
stick)
Optional Components: • Docking station with power cord and external power
supply unit (Note: The docking station is delivered in a
storage case.
• 1x Ethernet cable (crossover)
• Validation kit containing the following Raman reference
standards: 1x vial cyclohexane, 1x vial naphthalene,
1x vial benzonitrile, 1x vial tylenol, 1x PE bag tylenol
with certificate-of-origin
• Bruker validation manual
• optional OPUS software packages (e.g. OPUS/IDENT)
• Data system
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