Bruker NMR CryoFIT User manual

CryoFIT
CryoProbe Flow Conversion System
Installation Manual
Version 003
Innovation with Integrity
●
NMR

Copyright © by Bruker Corporation
All rights reserved. No part of this publication may be reproduced, stored in a retrieval
system, or transmitted, in any form, or by any means without the prior consent of the
publisher. Product names used are trademarks or registered trademarks of their re-
spective holders.
This manual was written by
T. Marquardsen, J. Ganz
© August 14, 2015 Bruker Corporation
P/N: H12214
For further technical assistance for this product, please do not hesitate to contact your
nearest BRUKER dealer or contact us directly at:
Bruker Corporation
Am Silberstreifen
76287 Rheinstetten
Germany
Phone: + 49 721 5161 6155
E-mail: [email protected]
Internet: www.bruker.com

Contents
H12214_1_003 3
Contents
1 About This Manual .............................................................................................................................5
1.1 Policy Statement .................................................................................................................5
1.2 Symbols and Conventions ..................................................................................................5
2 Introduction.........................................................................................................................................7
3 Transport, Packaging and Storage ...................................................................................................9
3.1 Symbols on the Packaging .................................................................................................9
3.2 Inspection at Delivery........................................................................................................10
3.3 Packaging .........................................................................................................................11
3.4 Storage .............................................................................................................................11
4 Flow Hardware ..................................................................................................................................13
4.1 CryoFIT Accessory (PZ H13485)......................................................................................14
5 Installation.........................................................................................................................................15
5.1 Installation with a Warm CryoProbe..................................................................................15
5.2 Installation with a Cold CryoProbe....................................................................................16
5.3 Assembling the CryoFIT Accessory..................................................................................16
6 Inserting the Flow Cell .....................................................................................................................19
7 Removing the Flow Cell ...................................................................................................................29
8 Dismantling and Disposal................................................................................................................33
8.1 Dismantling .......................................................................................................................33
8.2 Disposal ............................................................................................................................33
9 Contact ..............................................................................................................................................35
List of Figures...................................................................................................................................37

Contents
4 H12214_1_003

About This Manual
H12214_1_003 5
1 About This Manual
This manual enables safe and efficient handling of the device.
This manual is an integral part of the device, and must be kept in close proximity to the
device where it is permanently accessible to personnel. In addition, instructions concerning
labor protection laws, operator regulations tools and supplies must be available and adhered
to.
Before starting any work, personnel must read the manual thoroughly and understand
its contents. Compliance with all specified safety and operating instructions, as well as local
work safety regulations, are vital to ensure safe operation.
The figures shown in this manual are designed to be general and informative and may not
represent the specific Bruker model, component or software/firmware version you are
working with. Options and accessories may or may not be illustrated in each figure.
1.1 Policy Statement
It is the policy of Bruker to improve products as new techniques and components become
available. Bruker reserves the right to change specifications at any time.
Every effort has been made to avoid errors in text and figure presentation in this publication.
In order to produce useful and appropriate documentation, we welcome your comments on
this publication. Support engineers are advised to regularly check with Bruker for updated
information.
Bruker is committed to providing customers with inventive, high quality products and services
that are environmentally sound.
1.2 Symbols and Conventions
Safety instructions in this manual are marked with symbols. The safety instructions are
introduced using indicative words which express the extent of the hazard.
In order to avoid accidents, personal injury or damage to property, always observe safety
instructions and proceed with care.
DANGER
DANGER indicates a hazardous situation which, if not avoided, will result in
death or serious injury.
This is the consequence of not following the warning.
1. This is the safety condition.
uThis is the safety instruction.

About This Manual
6 H12214_1_003
WARNING
WARNING indicates a hazardous situation, which, if not avoided, could result
in death or serious injury.
This is the consequence of not following the warning.
1. This is the safety condition.
uThis is the safety instruction.
CAUTION
CAUTION indicates a hazardous situation, which, if not avoided, may result in
minor or moderate injury or severe material or property damage.
This is the consequence of not following the warning.
1. This is the safety condition.
uThis is the safety instruction.
NOTICE
NOTICE indicates a property damage message.
This is the consequence of not following the notice.
1. This is a safety condition.
uThis is a safety instruction.
SAFETY INSTRUCTIONS
SAFETY INSTRUCTIONS are used for control flow and shutdowns in the event
of an error or emergency.
This is the consequence of not following the safety instructions.
1. This is a safety condition.
uThis is a safety instruction.
This symbol highlights useful tips and recommendations as well as
information designed to ensure efficient and smooth operation.

Introduction
H12214_1_003 7
2 Introduction
This document describes the installation of the CryoFITTM accessory, including the insertion
and removal of the flow cell.
Important Information
NOTICE
Risk of Material Damage Due to Improper Usage
Failure to observe the following safety warnings may result in material damage.
uNever try to spin the flow cell.
uNever use the sample lift if the flow cell is installed.
uDo not try to insert NMR sample tubes.
uDo not use the sample changer.
uDo not use the device with a BCU05 (freezing).
uMake sure the sample changer is not used during the flow application.

Introduction
8 H12214_1_003

Transport, Packaging and Storage
H12214_1_003 9
3 Transport, Packaging and
Storage
Retrofitting, repairs, adjustments or dismantling of the device must only be carried out by
Bruker Service or personnel authorized by Bruker. Damage due to servicing that is not
authorized by Bruker is not covered by your warranty.
3.1 Symbols on the Packaging
The following symbols are affixed to the packaging material. Always observe the symbols
during transport and handling.
Top The arrow tips on the sign mark the top of the
package. They must always point upwards;
otherwise the content may be damaged.
Fragile Marks packages with fragile or sensitive
contents.
Handle the package with care; do not allow the
package to fall and do not allow it to be
impacted.
Protect Against Moisture Protect packages against moisture and keep
dry.
Attach Here Lifting gear (lifting chain, lifting strap) must
only be attached to points bearing this symbol.
Center of Gravity Marks the center of gravity of packages.
Note the location of the center of gravity when
lifting and transporting.
Weight, Attached Load Indicates the weight of packages.
Handle the marked package in accordance
with its weight.
Permitted Stacking Load Indicates packages which are partially
stackable.
Do not exceed the maximum load-bearing
capacity specified on the symbol in order to
avoid damaging or destroying the content.

Transport, Packaging and Storage
10 H12214_1_003
Do not Damage Air-tight
Packaging
The packaging is air-tight. Damage to the
barrier layer may render the contents
unusable.
Do not pierce.
Do not use sharp objects to open.
Component Sensitive to
Electrostatic Discharge
The packaging contains components which
are sensitive to an electrostatic discharge.
Only allow packaging to be opened by trained
personnel.
Establish potential equalization before
opening.
Protect from Heat Protect packages against heat and direct
sunlight.
Protect from Heat and
Radioactive Sources
Protect packages against heat, direct sunlight
and radioactive sources.
Table3.1: Symbols on the Packaging
3.2 Inspection at Delivery
Upon receipt, immediately inspect the delivery for completeness and transport damage.
Proceed as follows in the event of externally apparent transport damage:
• Do not accept the delivery, or only accept it subject to reservation.
• Note the extent of the damage on the transport documentation or the shipper's delivery
note.
• Initiate complaint procedures.
Note:
Issue a complaint in respect to each defect immediately following detection. Damage
compensation claims can only be asserted within the applicable complaint deadlines.

Transport, Packaging and Storage
H12214_1_003 11
3.3 Packaging
About Packaging
The individual packages are packaged in accordance with anticipated transport conditions.
Only environmentally friendly materials have been used in the packaging.
The packaging is intended to protect the individual components from transport damage,
corrosion and other damage prior to assembly. Therefore do not destroy the packaging and
only remove it shortly before assembly.
Handling Packaging Materials
Keep the original container and packing assembly, at least as long the warranty is valid, in
case the unit has to be returned to the factory. When the packaging material is no longer
needed dispose of in accordance with the relevant applicable legal requirements and local
regulations.
3.4 Storage
Storage of the Packages
Store the packages under the following conditions:
• Do not store outdoors.
• Store in dry and dust-free conditions.
• Do not expose to aggressive media.
• Protect against direct sunlight.
• Avoid mechanical shocks.
• Storage temperature: 15 to 35 °C.
• Relative humidity: max. 60%.
• If stored for longer than 3 months, regularly check the general condition of all parts and
the packaging. If necessary, top-up or replace preservatives.
Under certain circumstances, storage instructions may be affixed to packages which expand
the requirements specified here. Comply with these accordingly.

Transport, Packaging and Storage
12 H12214_1_003

Flow Hardware
H12214_1_003 13
4 Flow Hardware
For protection during transport the flow cell is shipped together with the CryoFIT, inside the
CryoFIT box, but is not mounted. For instructions for installing the flow cell refer to the User
Guide Handling of Flow Cell and Capillaries.
Figure4.1: The CryoFIT Accessory Box
NOTICE
Material damage from improper handling of the CryoFIT
Removing the screws from the spinner adapter at the end of the CryoFIT may result in
misalignment of the flow cell.
uDo not remove the screws from the spinner adapter at the end of the CryoFIT.
NOTICE
Material damage to the CryoFIT flow cell
The flow cell is very fragile and may be damaged when sliding the lift down when the
CryoFIT is not installed.
uSlide the lift down very slowy and carefully.
NOTICE
Material damage to the flow cell during storage and handling.
Storing and handling the assembly with the flow cell extended may result to damage or
misalignment of the flow cell.
uDo not handle the assembly when the flow cell is extended.
uDo not store the assembly with the flow cell extended.

Flow Hardware
14 H12214_1_003
4.1 CryoFIT Accessory (PZ H13485)
The CryoFIT accessory includes the following parts:
Figure4.2: Fittings, Sleeves and Capillaries
HZ14201 PH CRYOFIT HOLDER CPL
H13563 CRYOFIT VALVE
H12214 PH CRYOFIT MANUAL
Z74395 CRP CRYOFIT LOCATING SPINNER CPL.
Z74385 CRP CRYOFIT AIR CONNECTOR T-PIECE CPL.
85988 FITTING LONG PEEK NUTS 10/32 WITHOUT FERRUL
85936 TUBING SLEEVES 785-825U
88385 TOOL HEX SCR KEY- T-HANDLE 3MM
84510 HOSE PTFE ID=0.8 AD=1.6
84584 PACKING CORRUGATED CARDBOARD-CARTON 2.3
HZ14633 PH CRYOFIT ANTI-ROTATION PROTECTION UNIT
HZ13395 LOGO AVANCE ONE&TWO BAY BRUKER EMBLE
88650 WZ DOUBLE OPEN ENDED WRENCH 2.5X3.2
86637 CAPILLARY HOSE-CUTTER

Installation
H12214_1_003 15
5 Installation
The installation of the CryoProbe for NMR applications with standard sample and flow cell
varies depending on whether the CryoProbe is warm or cold:
•Installation with a Warm CryoProbe [}15]
•Installation with a Cold CryoProbe [}16]
Assembling the CryoFIT Accessory [}16] is the same for either case.
5.1 Installation with a Warm CryoProbe
• Insert the CryoProbe into the magnet.
• Plug in the T-piece for the VT gas and capillary and connect the CABLE BVT-CRP (P/N
Z14278).
Figure5.1: Bottom of CryoProbe (left) and T-Piece (right)
• Mount the ATM unit or the mechanical Tuning & Matching adapter.

Installation
16 H12214_1_003
5.2 Installation with a Cold CryoProbe
Make sure firmware version crcoah or higher is installed before installation.
• Dismount the mechanical Tuning & Matching adapter or the ATM.
The error message: (152) Temp. controller #3 sensor missing will appear on the
display.
NOTICE!Proceed to the next steps without delay!
• Dismount the VT – gas tube from the CryoProbe.
• Plug in the T – piece for the VT gas and capillary on the CryoProbe.
• Mount the ATM unit or the mechanical Tuning & Matching adapter.
The CryoProbe is now ready again for use with NMR sample tubes. To use the flow cell,
follow the instructions in the following chapters of this manual.
5.3 Assembling the CryoFIT Accessory
Before using the CryoFIT Accessory the valve, bracket and holder unit must be mounted.
• Mount of the valve used to disable the spinning flow on the hose leading to the Shim
Upper Part:
Figure5.2: Valve Mounting

Installation
H12214_1_003 17
• Mount the HOLDER BRACKET on the shim upper part as shown:
Figure5.3: Bracket Mounting on the Shim Upper Part
• Assemble of the CryoFIT Holder Unit as shown:
Figure5.4: The CryoFIT Holder Unit
• Mount of the CryoFIT Holder Unit to Holder Bracket on the upper part of the shim system:

Installation
18 H12214_1_003
Figure5.5: Mounting the CryoFIT Holder on the Upper Part of the Shim System
• Adjust the length of the bracing holder on the magnet until the plate is horizontal.
Figure5.6: Height of the Bracing Holder

Inserting the Flow Cell
H12214_1_003 19
6 Inserting the Flow Cell
Before inserting the Flow Cell make sure the CryoFIT Accessory has been assembled
correctly as described in the section Assembling the CryoFIT Accessory [}16].
• Switch off the BCU05 to prevent freezing.
• Close the valve to disable the spinning flow. Move the slider to the closed position.
Figure6.1: Valve Parts
• Activate the sample lift.
CAUTION!Make sure the sample changer is not used during the flow application.
Figure6.2: Activating the Sample List
Note: The insert guide is needed to insert the capillary properly into the probe. The insert
guide looks similar to a classical sample spinner, nevertheless, never try to spin it!

Inserting the Flow Cell
20 H12214_1_003
• The insert guide slots have to be rotated circa 90° relative to each other, as shown in the
figure below. Insert the insert guide into the magnet.
Figure6.3: Inserting the Guide into the Shim Upper Part
Figure6.4: Switching Off the Sample Lift
• Unscrew the plug on the T – piece of the VT gas at the bottom of the probe.
Do not leave it open for more than 10 minutes.
Do not disconnect the VT gas hose.
Some VT gas will escape from the opened T-piece.