BT Lab Systems BT2202 User manual

A Geno Technology, Inc. (USA) brand name
1-314-991-6034 ♦ [e-mail]
Mini PCR Machine
Cat. No. BT2202

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IMPORTANT SAFETY INFORMATION
•The user needs to have a complete understanding of how the instrument will work before operating
the instrument safely. Before operating the instrument, please read this manual carefully.
•The operator should not attempt to open or repair the instrument, which would void your warranty
and may result in an electric shock. In case of repair, contact BT Lab Systems.
•This product is an indoor instrument.
•Before connecting the power supply, make sure the voltage of the power supply matches the
voltage required by the instrument and make sure that the rated load of the power outlet is not less
than the requirements of the instrument.
•The instrument should be used with the power adapter that is supplied with it. If a wrong power
adapter is used, it may cause permanent damage to the instrument.
•Hold the plug with hands when plugging and unplugging the power cord. When inserting the plug,
make sure that the plug is fully inserted into the socket. Do not pull the power cord when pulling out
the plug.
•If the power cord is damaged, it must be replaced with a power cord of the same type and
specification.
•This instrument should be placed in a place with low humidity, little dust and far away from water
sources and avoid direct sunlight and strong light source. The room should be well ventilated and
free from corrosive gas or strong magnetic field interference. Keep away from heating, stove, and
other heat sources. Do not place the instrument in damp or dusty places.
•Power off when not in use. If the instrument will not be used for a long period, unplug, and cover
with a piece of cloth to protect it from dust.
•Unplug the instrument from the electrical outlet immediately under the following conditions and
contact the supplier or have it attended by trained service personnel:
oThere is liquid spilling inside the instrument.
oThe instrument is exposed to rain or water
oThe instrument is not working properly, especially if there are any abnormal sounds or
smells.
Incompatible Solvents
•This instrument is not compatible with strong acid, alkali, and most organic solvents. If any comes in
contact, remove immediately.
•Salt solution can slowly cause corrosion on the metal parts and should avoid contact
•This device is not waterproof and must not get wet. Avoid any forms of liquid from entering the
instrument

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MAINTENANCE
Use a neutral soap solution to clean the holes in the base. Do not use strong bases, concentrated
alcohols, and organic solvent solutions.
There should be no other articles under the machine or its left and right heat dissipation windows.
After the machine is used for some time, dust will adhere to the heat dissipation window. It is essential
that it is cleaned up in time.
The module should be cleaned frequently. Once some reactant residues are accumulated in the module
cavity, it will affect the temperature response. It is recommended to wipe it regularly with cotton cloth.
INTRODUCTION
The Mini PCR Machine is an ultra-lightweight, ultra-thin thermal cycle gene amplification instrument.
It has a 5” TFT High-Definition, True Color, Full Touch LCD screen. The whole program displays in real
time with curved graphs and the parameter modification is intuitive and convenient. It uses the latest
generation of semiconductor technology, has excellent temperature accuracy, and its uniformity
ensures high quality experimental results.
KEY FEATURES
•Unique appearance, compact size with interface operation simple and convenient.
•Built with the latest generation of semiconductor technology, excellent augmentation
performance, effectively eliminate the edge effect of module heat conduction, the module
temperature uniformity is excellent.
•The bottom shell with one aluminum carved processing is not only exquisite and solid, but it also
greatly enhances the heat dissipation performance.
•Has a 5” TFT HD full-touch color screen for a convenient and fast setting.
oCan quickly edit the required files.
oVisual accurate display of temperature curve and instrument running process status in
real time.
•The system has User login, password protection, data security, large data storage (the machine
can store more than 100 files). Administrators can remove users and files that are not required.
•The ingenious elastic hot cover structure design adapts to the different heights of test tubes,
guaranteeing the best conditions for the test
•Real-time temperature display, conducive to controlling the sample temperature.
•The hot lid temperature and working mode can be set. The hot lid can be switched on and off.
•The test tube and module temperature control modes can be chosen to meet different
experimental requirements.

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TECHNICAL SPECIFICATIONS
Normal Working Conditions
•Ambient Temperature: 5°C ~ 30°C
•Relative Humidity ≤70%
•Power Supply: AC110V/AC220V 50Hz
Basic Parameters and Performance
Single-Step Time Range 1-59m59s (0 is forever)
Temperature Range 4 – 99.9˚C
Sample Capacity 16 x 0.2ml
Max. Heating Rate 5˚C/s
Max. Cooling Rate 4˚C/s
Temp. Uniformity ±0.20˚C
Temp. Accuracy ±0.10˚C
Temp. Display Resolution 0.1˚C
Temp. Control Method Block/Tube
Hot Lid Temp. Range 30 - 105˚C
Max. Steps of the program 30
Program Max. # of Cycles 99
Time increment/decrement -599/+599s
Temp. increase/decrease -9.9 ~ +9.9˚C
Program Pause Function Yes
16˚C Insulation Forever
LCD 5-inch, 800 x 480 Pixel
Program Storage Quantity >100
Communication Interface USB 2.0 (extension)
Input Power 12V, 9.9A
Dimensions (W x D x H) 200 x 230 x 85mm
Net Weight 3.2kg

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STRUCTURE
Hot Lid Button
Power Switch
Vent Window
LCD Screen
Hot Lid
Power Socket

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OPERATION GUIDE
Start Up
After the instrument is powered on, the LCD screen will light up. The welcome screen will appear, then
the main menu interface.
Run File Operation
The run file is the PCR amplification program. The running file consists of the temperature and the cycle
steps. Each file can contain up to 30 steps.
1. Access File Library
a. Press on the “File” icon on the main menu to get to the file library interface.
The left column shows the list of files. The right side shows the specific file information
and file preview of the selected file. The bottom row consists of the function buttons.
When a file is selected, it can be edited, deleted, and renamed.

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2. Create a New File
a. Press “New” to enter the new editing interface. Above the temperature curve is the
temperature and below is the time.
b. Press on the temperature or time to set its parameters by the number keys
c. Press the “+Step” button and the “+Cycle” button to add temperature and cycle steps.
(Maximum number of steps is 30)
d. After setting the parameters have been completed, press the “Save” button.
e. Enter the file name, then press “OK” to return to the file library interface.

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3. Edit File
a. Select the file to be edited in the file library, then press on the “Edit” button. Press on
the temperature or time to edit.
b. Press the “+Step” button and the “+Cycle” button to add additional temperature and
cycle steps. (Maximum number of steps is 30)
c. Select a temperature step, then press the “Option” button to set detailed parameters
for that step, including “Temp”, “Time”, “+Temp/c” and “+Time/c”.
d. Press “OK” once finished with setting detailed parameters to return to the editing
interface.
e. Press “Return” to return to the file library interface.

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4. Running File
a. Select the file to be run in the file library. Press “Run” to enter the running interface.
b. Press “Lid Off” or “Lid On” to switch the hot cover.
c. The column to the right of the graph shows the hot lid and module temperature, the
current step time, and the total time.
d. Press “Run” and the file will start running.
When the file is running at a certain step, the temperature curve will flash to indicate
that it is running.
During the operation, the following functions can be used.
i. “Stop” – Stop the operation
ii. “Run” – Continue the operation
iii. “Pause” – Pause the operation
iv. “Skip” – Skip the next operation
After the running file time is over, the device enters a cryostat state, and the Total Time
displays complete. Press “Stop” to stop running.

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User Management
User Management allows new users to be created. Only administrator level users can delete other
users.
1. Press the “User” icon in the main interface to enter the user management interface
2. Press “Register” to enter the registration interface.
3. Enter the username, password, and other information, then press “OK” to complete
4. Press “Login” to enter the login interface. Input the corresponding information to complete
the login.
System Settings
1. Press on the “System” icon in the main interface to enter the system settings interface.
2. After setting is complete, press “OK” to save the settings, then press “Return” to return to
the main menu.