CMA Dishmachines 11 User manual

Instructions for CMA 11 Microdialysis Probe
1. Fill a microsyringe with perfusion uid and mount it in the CMA Microinjection Pump. The Perfusion Fluid must be
clean, at room temperature and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula.
3. Mount the microdialysis probe in the CMA 11 & 12 Probe/Guide Clip on the CMA/130 in vitro Stand. Connect the de-
sired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet. Tubing Adapters
and FEP-Tubing should be used for all connections. Put the microdialysis probe in a vial lled with perfusion uid. To
facilitate the handling of Tubing Adapters, they should be presoaked in ethanol for a minimum of 10 minutes.
4. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the cannula.
5. Flush the probe, lowered in a vial lled with 70% ethanol. Pump the perfusate for 4-5 min. to wash out the glycerol.
6. Put the probe back into the vial lled with perfusion uid. Flush for another 4-5 min. to wash out the ethanol and air. Use
the following ow rates:
Check for air bubbles inside the membrane with a stereomicroscope. The membrane may appear to be “sweating” which
is due to the ultraltration of uid through the membrane.
7. Set the pump to the required perfusion ow (usually 1-5 µL/min.) and check for leaks. The microdialysis probe is now
ready for use.
8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL
INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short
sampling times.
9. After the experiment, put the microdialysis probe in a vial lled with deionized water. Perfuse with deionized water to
prevent salt crystal formation.
NEVER EXCEED STATES FLOW RATES!
TECHNICAL INFORMATION
Membrane
Material Cuprophane
Molecular Cut-O 6,000 Daltons
Outer Diameter 0.24 mm
Length 1, 2, 3 and 4 mm
Probe Shaft
Material Stainless-steel or Metal Free
Diameter 0.40 mm
Length 14 mm
Internal Volume
Inlet Volume Negligible
Outlet Volume 1 µL
CMA 11 Microdialysis Probe User’s Manual
0.40
0.24
shaft
steel shaft
outletintlet
membrane
Flow Probe outlet tubing
50 μL/min 100 mm long
40 μL/min 200 mm long
15 μL/min 300 mm long

WARRANTY
The probes manufactured by CMA Microdialysis are warranted to be free from defects in material and workmanship for a
period of two years from the manufacturing date if stored in the original package.
Claims should be forwarded without delay to CMA Microdialysis or to your local distributor.
ORDER INFORMATION Ref No.
CMA 11 Microdialysis Probe, 1 mm, 3/pkg CMA 8309581
CMA 11 Microdialysis Probe, 2 mm, 3/pkg CMA 8309582
CMA 11 Microdialysis Probe, 3 mm, 3/pkg CMA 8309583
CMA 11 Microdialysis Probe, 4 mm, 3/pkg CMA 8309584
CMA 11 Probe Metal Free, 1 mm, 3/pkg CMA 8011081
CMA 11 Probe Metal Free, 2 mm, 3/pkg CMA 8011082
CMA 11 Probe Metal Free, 3 mm, 3/pkg CMA 8011083
CMA 11 Probe Metal Free, 4 mm, 3/pkg CMA 8011084
CMA 11 Probe β-Irradiated, 1 mm, 3/pkg CMA 8011001
CMA 11 Probe β-Irradiated, 2 mm, 3/pkg CMA 8011002
CMA 11 Probe β-Irradiated, 3 mm, 3/pkg CMA 8011003
CMA 11 Probe β-Irradiated, 4 mm, 3/pkg CMA 8011004
CMA 11 Guide Cannula, 3/pkg CMA 8309017
CMA 11 Guide Cannula, 30/pkg CMA 8309018
CMA 11 Guide Metal Free, 3/pkg CMA 8011085
CMA 11 Guide β-irradiated, 3/pkg CMA 8011031
CMA 11 Guide β-irradiated, 30/pkg CMA 8011032
Tubing Adapter, 10/pkg CMA 3409500
FEP Tubing, 1 m, 1/pkg CMA 3409501
FEP Tubing, 1 m, 10/pkg CMA 8409501
Tubing Connector, 3/pkg CMA P000113
CMA 11 & 12 Probe Clip CMA 8309013
The CMA 11 Microdialysis Probe is not intended for use in humans. It is only suitable for laboratory research in animals.
CMA Microdialysis only guarantees single usage of CMA 11 Microdialysis Probes
CMA Microdialysis AB
Head Oce, Sweden
Torshamnsgatan 30A, SE-164 40 Kista, Sweden
Tel: [phone]
E-mail: [email protected] • www.microdialysis.com
Harvard Apparatus
84 October Hill Road
Holliston, MA 01746 USA
Phone Orders: 800-232-2380 • Fax: [phone]
E-mail: [email protected] • www.harvardapparatus.com
D000458E