Cole Parmer SI-200 Series User manual

Cole-Parmer®
SI-200 Series
Incubators
Instruction Manual
SI600-89 Version 2.4
start/stop mode
rpm time on/off
press
to start
temperature ºC
on/off
on/off
pressto set
temperatureºC
on/off
time
rpm
start/stop mode

start/stop mode
rpm time on/off
press
to start
temperature ºC
on/off
start/stop mode
rpm time on/off
press
to start
temperature ºC
on/off
Temperature
control panel
Speed control
panel
USB connector
Locating holes for
tube accessory racks
Retractable
platform Retractable
platform
release knob
English

1
SHAKING INCUBATORS
SI-200D, SIC-200D-L and
SIC-200D-C
Instructions for use
1. Introduction
Thank you for purchasing this piece of
Cole-Parmer equipment. To get the best
performance from this equipment please read
these instructions carefully before use. Before
discarding the packaging check that all parts are
present and correct.
Item number Description
HH179(S) UK mains lead with plug
HH180(S) EU mains lead with plug
SI600-89 Instruction manual
I/FCALUSB USB cable
RSSL1 or SI600/1 Securing bars (4)
7001096 Guarantee card
SI600-214 Foot brackets (2) and
screws (4) for
SIC-200D-L and
SIC-200D-C
SIC-200D-C only:
SI600C-232 Washer (2)
SI600C-233 90° connectors (2)
SI600C-240 Condensate tubing (2)
SI600C-241 Coolant tubing (2)
SBS4074 Jubilee clip (1)
700198 Jubilee clips (4)
For your own safety and that of others please
read and understand the safety advice given
below before using the equipment.
2. Safety advice before use
If the equipment is not used in the manner
described in this manual and with
accessories other than those recommended
by Antylia Scientific, the protection
provided might be impaired.
This equipment is designed to operate under the
following conditions:
❖ For indoor use only
❖ Use in a well ventilated area
❖ Ambient temperature range +5ºC to +40ºC
❖ Altitude to 2000m
❖ Relative humidity not exceeding 80%
❖ Mains supply fluctuation not exceeding 10%
❖ Over-voltage category II IEC60364-4-443
❖ Pollution degree 2
Operational warnings and conditions:
CAUTION: HEAVY OBJECT. DO NOT
ATTEMPT TO LIFT OR CARRY THE UNIT
ALONE.
❖ Use with a minimum distance all around of
200mm (100mm at the rear) from walls or
other items.
❖ Not to be used to heat or mix materials which
could result in liberation of gases, or cause
explosions or implosions.
❖ Not to be used or placed in areas known to
be hazardous.
❖Never move or carry the unit when in use or
connected to the mains electricity supply.
❖When lifting or moving the unit, always lift
from the base.
❖Do not use in a hazardous atmosphere or
with hazardous materials.
❖Ensure that the load is balanced.
❖Do not attempt to stop movement by hand.
❖Do not lean on the open door of the
SIC-200D-L or SIC-200D-C as this could cause
the unit to tilt, particularly when the platform
is extended. Furthermore, do not leave the
incubator unattended with the door open.
❖Fitting of feet brackets to the SIC-200D-L and
SIC-200D-C is required prior to use (see 4.4).
❖In the case of mains interruption, the unit will
not restart on restoration of the electricity
supply. In the case of mechanical interruption,
(e.g. motor stall), the unit will continue
operating on removal of interruption.
❖Mechanical energy can lead to breakage of
glass vessels. Use with care.
USE CAUTION WHEN REMOVING VESSELS
FROM THE INCUBATOR AS PARTS MAY BE
HOT.

3. General Description
The Cole-Parmer SI-200D, SIC-200D-L and
SIC-200D-C orbital incubators are ideal for
mixing and aerating various biological samples
under accurate temperature controlled
conditions. The rotating platform has a digital
speed setting providing a smooth, uniform
circular motion with an orbit of 16mm. The
speed range is 30 to 300rpm. There is also an
adjustable digital countdown timer that
automatically stops after the set time (1 second
steps to 1h, 1 minute steps to 24h or 1h steps to
9 days). The shaking platform has a drawer
mechanism for easy access to the incubation
vessels. The temperature is controlled by a simple
“press to set” system in 0.1ºC intervals from
ambient +5ºC to 60ºC.
The model SIC-200D-C has the additional option
of connecting to a recirculating chiller unit
allowing for applications where sub-ambient
temperature conditions (ambient -15°C,
minimum 5°C) are required.
The incubators come fitted with a fully
adjustable cradle system to accommodate a wide
range of flasks and bottles. Optional accessories
include racks to accommodate 1.5ml, 15ml and
50ml tubes.
4. Preparation for use
4.1 Electrical installation
THIS INSTRUMENT MUST BE EARTHED
Before connecting the instrument please read
and understand these instructions and ensure
that the line supply corresponds to that shown
on the rating plate. The instrument is designed
for use on 230V 50Hz. The power consumption
of the unit is 300W for the SI-200D and 500W
for the SIC-200D-L and SIC-200D-C. Ensure that
a mains supply lead with the correct ratings for
the product is used. The instrument is fitted with
an IEC socket at the rear of the instrument for
connection of the mains lead. The mains supply
lead is the means of disconnection for the
instrument.
2
Caution: Fuses are fitted in both live and neutral
lines.
Fuses:
230V - 3.15A anti surge fuse. For all other
voltages always replace with an equivalent fuse
type and rating as those being removed,
alternatively see rating plate for fuse type and
rating.
This unit is supplied with two mains leads fitted
with IEC plugs for connection to the instrument.
One lead has a U.K. 3 pin plug and the other has
a 2-pin “Schuko” plug for connection to the
mains. Choose the lead appropriate for your
electrical installation and discard the other.
Should neither lead be suitable, take the lead
with the U.K. plug and replace the plug with a
suitable alternative. This involves cutting off the
moulded plug, preparing the cable and
connecting to the rewireable plug in accordance
with its instructions.
IT IS IMPORTANT THAT THIS OPERATION SHOULD
ONLY BE UNDERTAKEN BY A
QUALIFIED ELECTRICIAN
NOTE: Refer to the equipment’s rating plate to
ensure that the plug and fusing are suitable for
the voltage and wattage stated. The wires in the
mains cable are coloured as follows:
LIVE - BROWN
NEUTRAL - BLUE
EARTH - GREEN/YELLOW
The appropriate mains lead should be connected
to the instrument BEFORE connection to the
mains supply. Should the mains lead need
replacement a cable of 1mm2of harmonised
code H05VV-F connected to an IEC320 plug
should be used. N.B. the U.K. mains lead is
protected by a 10A fuse mounted in the plug
top.
IF IN DOUBT CONSULT A QUALIFIED
ELECTRICIAN
4.2 Positioning
Place the unit on a strong, sturdy, level, non-slip
surface ensuring that there is sufficient free
space on all sides without coming into contact
with anything during use. Allow at least 100mm
at the rear for easy access to the mains power

3
WARNING:
❖ Do not use flammable coolants.
 ❖ Consult the chiller manual to ensure a
compatible fluid is used.
 ❖ The fluid must be compatible with the
incubator’s wetted parts which are
brass, copper, EPDM rubber and nickel.
When using coolant below 0°C, there is the
potential for condensate to form on the tubing.
This can be reduced by insulating the tubing.
Ensure all tubing is kept free of kinks and
restrictions and is held tidily in place using tie
wraps if necessary. Check for leaks by starting up
the chiller pump before finally fixing the
SIC-200D-C in position on the bench.
4.4 Fixing feet brackets (SIC-200D-L and
SIC-200D-C)
To prevent the unit from vibrating or moving
during use, the SIC-200D-L and SIC-200D-C must
be secured to the bench top using feet brackets.
Two feet brackets are provided for securing the
rear feet.
Ensure the unit is positioned correctly. From the
sides of the unit, slide the feet brackets around
1
2
each of the rear feet (1). Secure in place by fixing
with two No.8 x 20mm screws (2).
Connect to the electricity supply - DO NOT
SWITCH ON.
4.4 Loading
Caution: keep loose items clear of the door before
opening.
Open the door. Pull the two black sprung release
knobs either side of the cradle outwards towards
the sides of the incubator and draw the cradle
supply lead and 200mm at each side of the
instrument.
4.3 Connecting the SIC-200D-C to a recirculating
chiller
In order to incubate at below ambient conditions,
the SIC-200D-C must be connected to a
recirculating chiller unit. Ensure that the chiller
unit is situated on a solid surface and does not
vibrate when the incubator is operating at
maximum rpm. Connect the condensate tubing
to the condensate outlet pipe and the other end
to a waste collection container positioned lower
than the condensate outlet pipe. Ensure the
condensate collection container can hold a liquid
volume at least as great as the chiller coolant
volume.
Attach the 90° connectors supplied to the two
connectors on the rear of the unit labelled INLET
and OUTLET. Connect the 1.5m lengths of 8mm
I.D. EPDM tubing to the connectors and secure
with jubilee clips. Connect the other ends of the
connectors to the ports of the chiller unit
ensuring there are no kinks in the tubing to
restrict flow; do not over-tighten.
Optimum performance will be obtained with the
connection tubing as short as possible. Observe
the specification of the chiller for acceptable
tubing lengths and maximum head height. Place
the chiller in a position which is safe in order to
prevent accidental collision.
Coolant
For temperatures below 10°C, or as
recommended in the chiller manual, a coolant
must be used. A mix of 1:1 laboratory grade
ethylene glycol and water is recommended. DO
NOT use deionised water.
Outlet port
Condensate
outlet pipe
Condensate
collection
container
Inlet port
To chiller
90° Connectors