Connections 1500 Series User manual

Proc. #715000266, Rev. A Page 1 of 3
SM
Waters 1500 Series Manual Injector Installation
This procedure describes how to install a Waters 1500 Series Manual Injector (Rheodyne 7725i)
on a 1500 Series pump. A mounting plate comes pre-installed in the pump; it will have to be
removed and modified before you can mount the manual injector onto the mounting plate. After
adjusting the fluidic tubing to fit, you will have to put the mounting plate back onto the pump and
finish connecting the fluid lines and electrical connections.
Parts Required
The Waters 1500 Series Manual Injector Kit (Part No. 186000872) consists of:
Part Number Quantity Item
WAT005063 1 Ferrule, Stainless Steel, 10 Pack
WAT005070 1 Compression Screw, 10-Pack
WAT020321 1 Cable, Event
WAT025462 2 Screw, 8-32X06, Binder
WAT033381 1 S
y
rin
g
e, 25
µ
l
WAT055840 1 Injector, Manual, 7725i
WAT055857 1 Kit, Sample Loop
415000250 1 Drip Tray, Valve, Manual Inject
430000406 1 Assy, Tube, Reference Valve To Manual Inject Valve
430000407 2 Assy, Tube, Manual Inject Valve To Waste
430000408 1 Assy, Tube, Manual Inject Valve To Column
Tools Required
•Phillips head screwdriver
•1/4-5/16 inch open-end wrench (WAT022527), provided in the 1515/1525 pump startup kits
•5/32-inch Allen wrench, provided in this kit
•Adjustable wrench, provided in this kit
NOTE: Install before power-up
Waters 1500 Series Manual Injector Installation
1. Unpack the manual injector kit and identify the four (4) pieces of stainless steel tubing.
Familiarize yourself with the ports on the back of the injector; refer to the flow diagram in the
Rheodyne literature that came with the injector and with Figure 1.
2. Assemble the tubing to the back of the injector, only hand-tightening the ferrrules at this time,
per Table 1.

Proc. #715000266, Rev. A Page 2 of 3
SM
Table 1 – Tubing Connections
430000406 - from the reference valve to P2.
430000407 - from V5 to waste.
430000407 - from V6 to waste.
430000408 - from C3 to column.
Figure 1 - Manual Injector, Rear View
3. Remove the mounting panel from the pump by unscrewing two captive screws, top and
bottom, as shown in Figure 2.
Figure 2 - Mounting Bracket Screw Figure 3 - Loosening Handle
4. Remove the hole cover by removing the two nuts on the rear of the mounting panel with the
adjustable wrench.
5. Remove the handle from the injector by loosening two Allen screws with the Allen wrench
provided, as shown in Figure 3.
6. Attach the injector to the mounting panel using two screws provided. Make sure the handle
moves between the 12 o’clock and 2 o’clock positions.
7. Replace the handle by tightening two Allen screws with the Allen wrench provided.
8. Check that the tubing will easily fit into the injector opening in the pump's front panel when
the panel is replaced. Adjust the tubing until it fits, then tighten the ferrules on the back of the
injector.
9. Re-attach the mounting panel to the pump by screwing in the two captive screws.

Proc. #715000266, Rev. A Page 3 of 3
SM
Figure 4 – 1500 Series Pump (1525 Shown)
10. Plug the two-wire connector into the socket on the pump's front panel.
11. Install the short piece of tubing into the upper right hole of the reference valve. Tighten with
the 5/16” open end wrench.
12. Install the two pieces of tubing with no ferrule into the waste tube at the bottom right of pump.
13. Run the remaining piece of tubing through the slot provided on the right hand edge of the
pump for the column connection.
14. To connect to a detector, refer to the Waters 1500 Series HPLC Pump Installation and
Maintenance Guide.
Note 1: The manual injector must be in the LOAD position when starting the Breeze
software. The busLAC/E card may not boot if the injector is left in the INJECT position.
Note 2: When using the manual injector in conjunction with flow and/or compositional
gradients, the injector must be turned back to the LOAD position before the end of the
run. Leaving the injector in the INJECT position automatically triggers the gradient table
for subsequent injections.
When using the 2410 refractometer with the manual injector (Rheodyne 7725i), the 2410
does not start acquisition until the injector is turned back to the LOAD position.
You will often have to leave the manual injector in the INJECT position for longer than a
few seconds. For example, if you are using a 2 ml sample loop and the flow rate is 1
ml/min, it will take 2 minutes to clear the loop.