CoolLED pE-100 Series User manual

DOC-015 Iss 1 1
pE-100 series
Quick Start Guide
This guide should provide some basic information on the setup and operation
of the CoolLED pE-100 Illumination System.
Before operating this product please read and observe the advice given in the
safety section of the full User Manual.
For more detailed information on the full range of options and functions
please see the full User Manual.

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1. Contents
A typical pE-100 (direct fit)
order will include the parts
shown. A microscope
adaptor that was specified
when ordering will be
fitted to the pE-100 Light
Source. A mains power
cable for the specified
region of use shall also be
included (not pictured).
If the liquid light guide
version is being used then
a specified light guide will
be fitted.
The fibre version of the pE-100 is a fixed module that allows connection to any SMA
terminated fibre. An appropriate fibre can be ordered through CoolLED if required.
2. Fitting the Light Source to a microscope
Attach the Light Source to the specified port of the microscope (Direct fit).
If using a liquid light guide version, attach to the microscope with appropriate
collimating optics.
pE-100 Light
Source
pE-100
Control Pod
pE-100
Power Supply
Epi-Fluorescent port
Transmitted (Brightfield) port

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3. Connecting the Control Pod and power supply
Connect the Control Pod cable with the D-
type connector to the Light Source.
Secure the connector in place with the
screws, using a flat-headed screwdriver.
Insert the connector from the power
supply into the Control Pod as shown.
Insert the mains lead connector into the
power supply.
Insert the mains plug into an appropriate
socket and switch on.

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4. Control Pod operation
5. Optical adjustment (direct fit version)
To optimize the Light Source set
up a sample that covers the entire
field of view.
Loosen the thumbscrew and slide
the Light Source back and forth
whilst viewing the sample.
Once you achieve maximum
brightness with an even
illumination secure the position by
tightening the thumbscrew.
On/Off status
Excitation intensity
level (%)
Light Source
wavelength (nm)
On/Off
button
Intensity control
buttons
Thumbscrew
Example of the effect of the adjustment on the sample
Light Source too far in
Light Source in optimal
position
Light Source too far out