CoolLED pE-400 Series User manual

DOC-075 Iss 1
User Manual
pE-400 Series: pE-400 and pE-400max

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DOC-075 Iss 1 i
Table of Contents
1. Introduction........................................1
2. Safety.................................................1
3. pE-400 Series Product Range ................2
3.1. Light Source Models .........................2
3.2. Wavelength Variants ........................2
3.3. Light Delivery Variants......................3
4. System Components ............................3
5. Reference Diagrams.............................4
5.1. pE-400 Light Sources ........................4
5.2. pE-400max Light Sources....................7
6. Installation ....................................... 10
6.1 Mounting the Light Source ..............10
6.2. Electrical Connections ....................12
7. Light Source Control .......................... 13
7.1. Manual Control Pods ......................13
7.2. TTL Triggering.................................18
7.3. Software ........................................18
7.4. pE-400max LightBridge.....................18
7.5. Sequence Runner (pE-400max) .........21
8. Excitation Filters (pE-400max) .............. 24
8.1. Excitation Filter Installation............24
9. Optical Adjustment............................ 26
10. Product options and Order Codes ..... 27
11. Warranty and Repairs ...................... 27
12. Compliance ..................................... 28
WEEE ....................................................28
RoHS .....................................................28
13. Recycling......................................... 28
14. Contact Details ................................ 29
15. Product Specifications...................... 29
16. Appendix ........................................ 31
16.1. Boot Load Procedure ....................31

DOC-075 Iss 1 1
1. Introduction
Congratulations on the purchase of your new CoolLED pE-400 Series Illumination System.
The pE-400 Series is a family of state-of-the-art four-wavelength LED Illumination Systems
designed to meet the demands of cutting-edge microscopy applications.
This User Manual provides all the information required for installation and safe operation.
For additional details as well as other valuable LED illumination resources, please see the
CoolLED website: www.coolled.com.
2. Safety
Warning: While LEDs are much safer than the mercury and metal halide lamps they
replace, the following precautions must be taken when operating your pE-400 Series
Illumination System. Failure to do so may result in permanent injury, damage to
property or both.
•Only use the power supply and power cord provided to power the device.
•Never look directly into the output of the Light Source or attached accessories. The
light can permanently damage the eye and may lead to blindness.
•Always ensure the Light Source is securely attached to the microscope before
applying power to the device.
•If the Light Source must be operated while not attached to a microscope, all
personnel must wear appropriate eye shielding and protective clothing. CoolLED
strongly discourages using any CoolLED Light Source when not securely mounted to a
microscope.
Warning: Optical surfaces must only be cleaned with specifically designed optical
wipes and purpose-formulated optical cleaning solutions. Failure to use optics-
specific cleaning products may permanently damage the CoolLED Light Source.

DOC-075 Iss 1 2
3. pE-400 Series Product Range
3.1. Light Source Models
The pE-400 Series includes two models: the pE-400 and the pE-400max.
The pE-400 is a simple, cost-effective white light source and controllable replacement for
mercury and metal halide lamps.
The pE-400max permits the independent adjustment of channel irradiance and the ability to
fit inline excitation filters.
3.2. Wavelength Variants
The pE-400 and pE-400max can be purchased in two wavelength variations: Single-Band (SB)
and Multi-Band (MB). The graph and Table 1 below detail the wavelengths available in the
pE-400 Series and the respective channel number.
Wavelength
Variant
Channel 1
Wavelength
(nm)
Channel 2
Wavelength
(nm)
Channel 3
Wavelength
(nm)
Channel 4
Wavelength
(nm)
Single-Band
(SB)
365
450
550
635
Multi-Band
(MB)
400
450
550
635
Table 1

DOC-075 Iss 1 3
3.3. Light Delivery Variants
The pE-400 and pE-400max are available with Direct Fit (DF) and Liquid Light Guide (LLG)
output couplings. Direct Fit Light Sources (Figure 1) are designed to be secured directly to
the epi-illumination arm of compatible microscopes, while liquid light guide units (Figure
2) accommodate a 3 mm light guide. The attached liquid light guide can be used as an
input to an optional collimator or as a direct input to compatible microscopes.
Figure 1 –Direct Fit Output
Figure 2 –Liquid Light Guide
Output
4. System Components
pE-400 Series Illumination Systems include various components necessary to meet the
demands of specific microscope installations. At a minimum, the pE-400 Series
Illumination System includes the following:
•A pE-400 Series Light Source
•A pE-400 Series Control Pod
•A power supply
•A 1.5 mm Hex Key required for installation
Figure 3 –(Anti-Clockwise) A pE-400max Control Pod, a pE-400 Series Power Supply, 1.5 mm Hex Key
and pE-400max Light Source.
1.5 mm

DOC-075 Iss 1 4
5. Reference Diagrams
5.1. pE-400 Light Sources
Direct Fit
Figure 4
Item
Description
1
Adaptor retention screws
2
Focus adjustment
3
On/off switch
4
Grounding terminal
5
Global TTL input (global shutter)
6
Control Pod socket
7
USB A socket
8
Power socket
9
Status indicator
10
Bootload switch
Table 2

DOC-075 Iss 1 5
Liquid Light Guide
Figure 5
Item
Description
1
On/off switch
2
Grounding terminal
3
Global TTL input (global shutter)
4
Control Pod socket
5
USB A socket
6
Power socket
7
Status indicator
8
Bootload switch
9
Liquid light guide retention screws
Table 3

DOC-075 Iss 1 6
pE-400 Control Pod
Figure 6
Item
Button Description
1
Mode
2
On/off
3
Increase global irradiance
4
Decrease global irradiance
Table 4

DOC-075 Iss 1 7
5.2. pE-400max Light Sources
Direct Fit
Figure 7
Item
Description
1
Adaptor retention screws
2
Focus adjustment
3
Filter slot 1
4
Filter slot 2
5
On/off switch
6
Grounding terminal
7
Channel TTL input 365/400 nm (channel shutter)
8
Channel TTL input 450 nm (channel Shutter)
9
Channel TTL input 550 nm (channel Shutter)
10
Channel TTL input 635 nm (channel Shutter)
11
Global TTL input (global shutter)
12
Control Pod socket
13
USB A socket
14
Power socket
15
Status indicator
16
Bootload switch
Table 5

DOC-075 Iss 1 8
Liquid Light Guide
Figure 8
Item
Description
1
Filter slot 1
2
Filter slot 2
3
On/off switch
4
Grounding terminal
5
Channel TTL input 365/400 nm (channel shutter)
6
Channel TTL input 450 nm (channel shutter)
7
Channel TTL input 550 nm (channel shutter)
8
Channel TTL input 635 nm (channel shutter)
9
Global TTL input (global shutter)
10
Control Pod socket
11
USB A socket
12
Power socket
13
Status indicator
14
Bootload switch
15
Liquid light guide retention screws
Table 6