Covaris S220 User manual

For research use only
S220 Series
USER MANUAL
Instrument and software developed for
high performance sample preparation
using adaptive focused acoustic energy
Product Name: S220-Series
Model Numbers: S220, S220x, S220R
Software: SonoLab 7

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Trademarks
Patented or patent pending and registered or registration-pending trademarks of Covaris are protected.
Registered names and trademarks used in this document, even when not specifically marked as such, are not to be
considered unprotected by law.
WCS and AFA are either registered trademarks or trademarks of Covaris, Inc. in the United States and/or other
countries.
Clorox is a registered trademark of The Clorox Company.
Teflon is a registered trademark of The DuPont Company.
Information subject to change without notice
For Research Use Only
Not for use in diagnostic procedures
Publication P/N 010122
Revision I, May 2017
Product covered by Patent No. US6, 719,449, US6, 948,843, and other pending applications
Copyright © 2017 Covaris, all rights reserved.
Covaris, Inc.
14 Gill St, Unit H
Woburn, Massachusetts
01801-1721 USA Printed in U.S.A.
UNIVERSAL PRECAUTIONS
Universal Precautions should be followed on all specimen samples, regardless of whether a sample is known to contain
an infectious agent. Laboratories handling specimen samples are advised to comply with applicable parts of the following
governmental and clinical standards, or their equivalent in the country of use:
Centers for Disease Control (CDC), Universal Precautions for Prevention of Transmission of HIV and Other
Bloodborne Infections, published 1987, updated 1996
Clinical and Laboratory Standards Institute (CLSI), GP17-A2 Clinical Laboratory Safety; Approved Guideline -
Second Edition, published 2004, ISBN 1-56238-530-5
Clinical and Laboratory Standards Institute (CLSI), M29-A3 Protection of Laboratory Workers from
Occupationally Acquired Infections; Approved Guideline, Third Edition, published 2005, ISBN 1-56238-5674
Occupational Safety and Health Administration (OSHA), 29 CFR 1910.1030 Bloodborne Pathogens
International Standards Organization (ISO) 15190:2003, Medical Laboratories –Requirements for Safety

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Warnings
For safety of operating personnel:
Make sure that the equipment is properly grounded. DO NOT operate if it is not properly grounded.
The unit is equipped with a power plug appropriate for the destination country. DO NOT, under any circumstances,
remove the grounding prong from the power cord.
Do not run an acoustic treatment with the Acoustic Assembly and sample cover in the UP position or without a water
bath - the acoustic system will not work.
If there is any indication that the Safety System is not functioning properly, DO NOT operate the equipment and
contact Covaris immediately.
If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment
may be impaired.
To prevent damage to the equipment:
The instruments are designed to operate in ambient laboratory conditions e.g., 19ºC to 25ºC (66ºF to 77ºF). DO
NOT operate the instrument in a cold room environment; the system is designed to operate with a water bath and
re-circulating heater/chiller apparatus to control sample temperature.
NEVER run a method without a water bath; this could damage the transducer. The instrument is equipped with a
water level sensor to protect the transducer and degassing pump. The system will not allow the degassing pump to
operate or the acoustic treatment to start unless an adequate volume of water is detected.
Distilled or deionized water should be used to fill the water bath.
Unless a Water Conditioning System (WCS) is employed, empty the water bath and wipe it dry EVERY day with a
lint-free cloth. DO NOT leave water in the tank for an extended time as there is no water filtration or water cleaning
system within the apparatus (unless WCS is employed).
Do not employ isopropyl alcohol, ammonia-based or abrasive cleaners on the water tank, as these will damage the
acrylic surfaces.
Establish a standard of operation and periodically test equipment, as described in Maintenance (see section 6.0) of
this manual.
Loading third party software onto the computer may interfere with system operation. Please consult with Covaris.

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Warranty
When used in accordance with written instruction and under normal operating conditions, the Covaris instruments
are guaranteed to be free of defects in MATERIAL and WORKMANSHIP for one (1) year from the date of original
delivery by an authorized representative. Any component which proves defective during the stated period will be
repaired free of charge or replaced at the sole discretion of Covaris, F.O.B., Woburn, Massachusetts, U.S.A.
provided the defective component is returned properly packaged with all transportation charges prepaid. The
customer is expected to perform basic diagnostics and component replacement with telephone support from Covaris
personnel. If Covaris personnel are required to perform on-site repair, all travel related costs are paid by the
customer. A limited warranty as specified may apply to certain components of the equipment.
Warranty Exceptions
This warranty is void if failure of the software or hardware has resulted from accidents, abuse, improper
maintenance, or repair, or misapplication by the customer. It is also void if damage is caused by any unauthorized
attachments or if modifications are made to the equipment. Removing or tampering with the Safety Enclosure will
void the warranty, and the customer will assume all liabilities.
This warranty is limited to the original purchaser and is not transferable.
The software will perform according to the accompanying written materials and the medium on which the software is
delivered is free of defects in materials under normal use and service. The warranty is void if damage has resulted
from third party software not intended for use with the system.
The high power focused transducer is designed to give maximal mechanical energy output in water. Permanent
damage to the transducer and electronic circuits could result if the transducer is operated without water. Operation
of the system without water in the water bath voids the warranty.
CONTACT COVARIS, INC. SHOULD YOU HAVE ANY QUESTIONS CONCERNING EQUIPMENT
Warranty Services
The purchased equipment is covered by a twelve (12) month warranty which includes all the service and support
necessary so that the customer can operate the equipment successfully. Extended warranties are available at the
end of the original 12 month warranty period.
Services included with the original purchase of the system are:
Technical Support –On-going assistance with the operation or application of the equipment and/or troubleshooting
is provided via:
Telephone [phone]) during the hours of 9:00am to 5:00pm, Monday through Friday,
oUnited States, Eastern Standard Time (EST)
oGreenwich Mean Time (GMT-05:00)
E-mail queries to [email protected]
Parts Replacement –Replacement of parts (excluding consumables) from normal operation of equipment are
provided on a priority basis. All labor and shipping charges are included. Failure due to accident, abuse, or
improper operation is not covered.
An Operator’s Manual is provided with the equipment. This manual includes sections on the operating
instructions, maintenance guidelines, and troubleshooting tips.

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TABLE OF CONTENTS
1.0 INTRODUCTION................................................................................................................................6
1.1 Overview of the User Manual.........................................................................................................6
1.2 The S220 and the Covaris Process ...............................................................................................6
2.0 INSTALLATION AND SETUP............................................................................................................7
2.1 Assembly........................................................................................................................................7
2.2 S220 Series Installation .................................................................................................................8
3.0 GETTING STARTED .......................................................................................................................10
3.1 Fill the Water Bath........................................................................................................................10
3.2 Start the System...........................................................................................................................10
3.3 The SonoLab Run Screen - Running a Method...........................................................................11
3.4 System Shutdown ........................................................................................................................14
3.5 Temperature Control....................................................................................................................15
3.6 Degas System..............................................................................................................................15
4.0 SonoLab™ APPLICATION SOFTWARE.........................................................................................16
4.1 Run Screen ..................................................................................................................................16
4.2 Create and Edit Methods .............................................................................................................17
4.3 Temperature Tab..........................................................................................................................21
4.4 Saving Methods in Folders...........................................................................................................22
4.5 History Tab...................................................................................................................................23
4.6 Setup Screen................................................................................................................................23
4.7 Maintenance Screen ....................................................................................................................28
4.8 About Tab.....................................................................................................................................29
5.0 SYSTEM SPECIFICATIONS ...........................................................................................................30
6.0 MAINTENANCE...............................................................................................................................31
6.1 Cooling Air Intake.........................................................................................................................31
6.2 Water Bath ...................................................................................................................................31
6.3 Transducer...................................................................................................................................31
6.4 Safety System..............................................................................................................................31
6.5 Degassing System .......................................................................................................................31
6.6 Cleaning the System....................................................................................................................31
7.0 TROUBLESHOOTING.....................................................................................................................32
Appendix A: Suggested S220 Series IQ / OQ / PQ ....................................................................................34
Appendix B: S Series Site or Transport Preparation...................................................................................40
Appendix C: Installing SonoLab Software, Firmware and Instrument Drivers ............................................41
Appendix D: Recirculating Chiller................................................................................................................46

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1.0 INTRODUCTION
1.1 Overview of the User Manual
This manual contains operation and maintenance instructions for the Covaris S220 Series Focused-
ultrasonicators (S220, S220x or S220R). In this document, “S220” generally refers to any of these
S220 Series of instruments. This document contains information essential to the proper use and
care of the equipment.
Should any unforeseen problems occur with the normal operation of the equipment, contact Covaris
Technical Support immediately.
The following definitions apply in this manual:
NOTE: Inconvenience if disregarded.
CAUTION: Equipment damage may occur.
WARNING: Personal injury may occur.
There is also a PDF version of this manual, typically available on the computer desktop or
downloadable from the Covaris website. Open the manual by double clicking the user manual icon.
1.2 The S220 and the Covaris Process
The S220 Focused-ultrasonicator is based on Covaris Adaptive Focused Acoustics (AFA)
technology. This instrument is designed to provide researchers an integrated stand-alone tool to be
used in a laboratory environment. The instrument is ideal for those who work with tissue
homogenization, cell lysis, DNA or chromatin shearing, and other sample preparation steps.
The system is comprised of the Treatment System which delivers the acoustic energy to the sample,
the Safety System which protects users from inadvertently contacting the high intensity acoustic
energy, and the Computer and Application Software which provides users an interface to control the
device.
The Covaris Process uses adaptive focused acoustic energy to precisely control cavitation and
acoustic streaming within the sample treatment vessel in a non-contact, isothermal fashion. Focused
acoustic energy is generated and delivered to individual samples. The mechanical energy imparted
on the sample results in a controlled series of compression and rarefaction events.

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2.0 INSTALLATION AND SETUP
2.1 Assembly
The following items should be found in the package of the Covaris S220 instrument:
Quick Start User Guide
User Manual
Notebook Computer with AC Adapter
Cables
oUSB cable
oS220 power cord
oComputer AC Adaptor power cord
Accessories
oChiller Hose Kit
oSample Tube Holders (may vary according to application)
oS220 Water Tank
oS220 Main Unit
Please note that the SonoLab software installer, if required, is available from the Covaris website or
by contacting Covaris technical support.
The S220 Focused-ultrasonicator

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2.2 S220 Series Installation
2.2.1 Unpack the S220 and place it on a table or bench, with space to accommodate the
instrument and the laptop. The recirculating chiller may be placed on the floor to save
bench space.
2.2.2 Please refer to the rear panel of the S220 and identify the following features:
The Rear Panel of the S220 System
2.2.3 Plug the S220 into a power source using the enclosed line cord. The S220 is a
“universal input” device and automatically employs any worldwide line voltage or
frequency (100 to 240 volts AC, 50 or 60 Hz). Leave the instrument power and
notebook power switched OFF at this time.
2.2.4 Plug one end of the USB cable into the USB Port, and the other end into any USB port
on the notebook computer.
2.2.5 Connect the computer to a power source. Connect the mouse or other accessories to
the computer.
USB Port
Expansion Port
Ventilation Fan
Product Safety Label
Serial Tag,
Regulatory and
Line Power Label
AC Line (Mains) Input
Chiller Connections

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2.2.6 Connect the two hoses provided for coolant flow from the chiller. The connections are
bi-directional. (There is no defined IN or OUT.)
2.2.7 Connect the hoses to the chiller. Please refer to Appendix D and your chiller operation
manual for details on chiller connection and operation.
2.2.8 Position the S220 and computer in your work space as desired.
CAUTION: When positioning the system, please keep the fan and rear panel vents
free of obstructions that may block the flow of air.
CAUTION: To prevent chiller damage, use of a 20% glycol solution in the circulating
chiller fluid is strongly recommended. Please refer to the chiller’s user manual.
2.2.9 Unpack the Water Tank.
2.2.10 At the front of the S220, lift the Acoustic Assembly, slide the Tank into the frame as
shown, and lower the Acoustic Assembly. The WATER LEVEL label should face
forward.
The S220 Acoustic Assembly and Water Tank
2.2.11 If a Water Conditioning System (WCS) will be employed with the S220 system, please
refer to the WCS User Manual for further instructions. The WCS removes biological
growth and particulates from the water, allowing bath water to remain in place for up to
one month.
2.2.12 The S220 system is now ready to be started.
Safety Cover
Acoustic Assembly
Water Tank

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3.0 GETTING STARTED
3.1 Fill the Water Bath
The S220 transducer must be immersed in a water bath to transmit high-
frequency, focused acoustic energy to the sample tube. For best performance,
employ only clean, distilled or deionized water in the bath.
Lift the Acoustic Assembly, remove the tank and fill to the level specified in the
protocol to be used. Typical DNA Shearing protocols call for water level 12.
Avoid filling higher than 15, as the tank may overflow. Employ the markings on
the FILL side of the gauge. The tank holds approximately 0.5 gallons (1.6 liters).
Once the tank is in place and the Acoustic Assembly is lowered into the bath, the
water level should be read from the RUN side of the label.
3.2 Start the System
Turn S220 power ON using the red power switch located on the instrument. When
power is ON, this switch will illuminate and the ventilation fans in the rear panel will
run.
Also start the external chiller, programming its set point to a few degrees below the desired S220
bath temperature. Be sure to employ a 20% glycol solution inside the chiller to prevent freezing. Do
not use undiluted glycol solution.
Start the system computer. The computer employs a Windows®Operating System (XP, 7 or 8).
SonoLab 7 is an application used to control a Covaris Focused-ultrasonicator for processing a
biological or chemical sample. It is designed to create, edit, and run a processing program called a
method. It has provisions to run, pause or stop a method and view execution histories which contain
a record of key instrument parameter values during a run. It also contains editors for customizing
system settings and performing maintenance functions.
SonoLab 7 is installed on the computer purchased with the S220 instrument. No further
installation or configuration is required.
Find the SonoLab icon on the desktop:
If the icon is not visible, or if SonoLab 7 is not installed, please refer to Appendix C or contact
Covaris for installation instructions.
Double-click on the icon to start SonoLab. An animated splash screen will appear:

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Following the splash screen, the SonoLab Run Screen will appear:
If the S220 is powered ON and connected to the laptop via the USB cable, SonoLab will connect to
the instrument, display its model type (e.g. “S220”) at the top center of the screen, and display the
message, “Connected to Instrument” via a COM port identified in the lower left corner.
If SonoLab does not connect to the instrument, the Run Screen will not appear. SonoLab will display
the About Screen and the status “Disconnected” will be displayed in place of the instrument model.
In this case, please refer to Section 7.0 for diagnostic instructions.
3.3 The SonoLab Run Screen - Running a Method
3.3.1 Instrument Status
System attributes are displayed on the Run Screen as green (acceptable) or red. The SonoLab
Run button will remain gray and inactive unless a method has been selected, the degas pump is
ON, and all five Status conditions are green (acceptable) or overridden. Any method will be
paused if any status changes to not acceptable while the method is running.
The status indicators and controls are described below:
Degas Pump: is both a control and indicator for the S220 degas pump. The pump is
automatically turned ON when SonoLab starts. When ON, the degas pump removes

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dissolved gas from the bath water. Clicking on the switch image will turn the pump OFF.
Clicking again will turn it ON.
Degas: To ensure properly degassed water, the degas pump should operate continuously
for at least 30 minutes. The Degas indication will turn green once this criterion has been
met. If the degas pump is turned OFF, this timer resets. Operators may click “Override” to
bypass this status check.
Water Temperature indicates if the bath temperature is between the minimum and
maximum set for the selected method. Operators may click “Override” to bypass this status
check. See the Temperature Control later in Section 3 and Appendix D for details on using
the external chiller to maintain bath temperature within desired limits.
Water Level indicates if there is enough water in the water tank for safe operation of the
instrument. Water levels near or below “0” on the WATER LEVEL label will cause this
indicator to turn red.
Water Tank: A green check indicates that the Acoustic Assembly is in its DOWN position
and that a water tank is present in the instrument.
Safety Cover: A green check indicates that the sample cover is properly closed.
n
3.3.2 Select a Method
To select and load an acoustic Method, click the pull-down menu of the “Method” combo box, as
shown below, and a list of existing method names along with their detailed information will
appear. Navigate through the list with the vertical scroll bar and click to select a Method.
NOTES:
Before running a method, load a sample tube into a holder, open the Safety Cover and
place the holder into the S220. Then, close the Safety Cover.
The S220 does not control its own water temperature. This must be controlled by adjusting
the set point of the external chiller.
For best results, allow the degas pump to run for at least 30 minutes, and the Degas
indicator turn green, prior to running a Method.
When an acoustic method is running, objects in the focal point of the transducer will be
exposed to high power, focused ultrasonic energy. Employ Covaris consumables at
recommended power levels, or containers capable of surviving these treatments.
Pull down menu
Edit the selected
method
Create a new method
Run/Creation Notes
(Click to expand)

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If no methods appear in the pull-down menu, then a new method must be created. Please see
Section 4 for instructions on creating and editing methods.
3.3.3 Run a Method
To start an acoustic treatment, seven conditions must be met:
There must be an acoustic method selected in the Method box
The Degas pump must be running
The Degas pump timer must see the pump running for 30 minutes (or override)
Water Temperature must be within acceptable Min and Max limits (or override)
The Water Tank must be present (and Acoustic Assembly down)
Water Level must be at or above that required for correct operation
The Safety Cover must be closed
The Run button will remain gray and inactive until all of these conditions
are met. Once a method has been selected and all system attributes
are acceptable or overridden, the Run button will become active
(green).
Click Run to start the selected method. Acoustic power will be applied to the sample tube
according to the acoustic parameters specified by the method. .
The programmed temperature range and the acoustic treatment details will be posted on the left
side of the Run Screen:
Average acoustic power (in Watts) and water bath temperature will be displayed numerically and
graphically at the lower right corner of the Run Screen, along with a progress bar for the method
being run:
Note that the average Power values will approximate (Peak Power x Duty Factor).

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The user may, at any time while running a method, click “Pause” to
interrupt a running method.
When the method is paused, the user may continue
the method by clicking the Resume button, or
terminate the method by clicking the Abort button.
When the method ends, a Run Results box will be posted over the Run Screen. If necessary,
click the mouse in the text entry area to set the focus, then notes may be added either via the
keyboard or an alternative input device. For instance, a barcode reader may be used to capture
data directly from pre-labeled samples.
When finished adding notes, click Close.
Simply open the Safety Cover and remove the sample tube.
A new sample tube may then be inserted into the holder and the process repeated.
If there is additional sample processing to be done, the S220 system and SonoLab may be left
ON and operating. If the system will be idle for an extended period of time (e.g. overnight or a
weekend), then please refer to the next section on System Shutdown.
3.4 System Shutdown
If the instrument will be idle for an extended period of time (e.g. overnight or a weekend), shut down
the instrument using the following procedure:
3.4.1 Empty the water tank and purge the degas pump
Turn off the degas pump and raise the Acoustic Assembly. Empty the water bath, place it back
into the instrument and lower the Acoustic Assembly.
Click the Degas Pump control to restart the pump and purge the pump and water lines. The
pump will automatically stop within 10 seconds. Remove and empty the tank again, dry the tank
with a lint-free soft cloth and place it back in the system.
3.4.2 Close SonoLab
Exit SonoLab by clicking on the red X in the upper right corner. ”Are you sure you want to exit
SonoLab?” will appear.
“Yes” will close the application. “No” will cancel this request.
3.4.3 Turn System OFF
To shut down the laptop, select Shutdown from the START menu on the Windows screen and
select the option SHUTDOWN. Then the computer will power down.
Power down the instrument using the illuminated switch located near the top of the instrument
next to the Covaris logo.
CAUTION: Leaving water in the water bath or degassing lines for an extended time
may promote algal growth in the tank and lines, This growth can interfere with water
quality and sample processing.
Change water daily and rinse with a 10% bleach solution monthly.

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3.5 Temperature Control
An important principal of the S220 Focused-ultrasonicator is isothermal temperature control. The
sample is immersed in a water bath which conducts ultrasonic energy while maintaining a stable
sample temperature. Each protocol published by Covaris specifies a desired bath temperature.
The S220 does not control its own water temperature. This must be controlled by adjusting the set
point of an external chiller. This chiller pumps fluid through hoses connected to the rear panel of the
unit (see Setup) and then through stainless steel tubes immersed in the water bath. These tubes
are visible as part of the Acoustic Assembly, just above the ultrasonic transducer.
The chiller set point should be programmed a few degrees C below the desired bath temperature.
For example, if a protocol calls for a bath temperature of 7°C, the chiller set point should be adjusted
to approximately 4°C.
During ultrasonic treatments, the S220 continuously measures water bath temperature and displays
this value in SonoLab. Each method has a minimum and maximum temperature defined, and
treatments will be paused if bath water strays beyond these limits. Using the example of 7°C, the
minimum and maximum may be set to 4°C and 10°C, respectively.
If the bath temperature exceeds the method’s programmed temperature limits (in either direction, too
cold or too warm), the Temperature Status indicator on the SonoLab Run Screen will turn red and
the method will pause (unless Temperature has its Override box checked). Such a deviation in
temperature may be indicative of a chiller malfunction or a flow failure in the chiller lines.
CAUTION: To prevent chiller damage, use of a 20% glycol solution in the chiller fluid
is required. Do not use undiluted glycol solution. Please refer to the chiller user
literature.
Use only deionized or distilled water in the instrument bath.
3.6 Degas System
The S220 is equipped with a pump that draws bath water through a degassing process. Properly
degassed water is important for efficient transfer of acoustic energy from the S220 transducer to the
sample being treated. If water is detected in the tank, the pump is automatically turned
on when SonoLab is started.
The pump should be allowed to run until the Degas indicator on the Run Screen turns
green (at least 30 minutes) prior to an acoustic treatment.
The pump may be switched off and on with the Degas Pump control on the Run Screen.
Chiller Tubes
Transducer

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4.0 SonoLab™ APPLICATION SOFTWARE
SonoLab is an application used to control a Covaris AFA (Adaptive Focused Acoustics) instrument
for processing a sample. It is designed to create, edit, and run a sample processing program called
a method. It has provisions to run, pause or stop a method and view execution histories which
contain a record of key instrument parameter values during a run. It also contains editors for
customizing system settings and performing maintenance functions.
SonoLab provide a series of tabs to access its various features. Each tab will highlight as the cursor
approaches, and a single mouse or touchpad click will access the new screen. Each screen has
secondary screens, indicators and controls. The choices are:
Run –Select methods to run, run methods, create new or edit existing methods
History –View system history
Setup –System setup by user (file management, User Authentication, etc.)
Maintenance –Instrument parameters set by factory or service personnel
About –System configuration information
4.1 Run Screen
The Run tab is the principal user screen for the system. See Section 3 for details on operating the
instrument through the Run Screen.
The Run Screen
The Run screen provides access to the Create New Method and Edit Existing Method screens.

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4.2 Create and Edit Methods
SonoLab supports the creation of new methods and editing of existing methods from the Run Tab. A
method consists of one or more treatments, delays and message prompts.
4.2.1 To edit or create a new method, click the “Edit…” or “New…” button in the “Method”
window. The Method Editor window will appear.
Controls for creating and editing a method
4.2.2 If creating a new method, in the Basic Settings tab, select the “Add Step” to create a
treatment.
Add Step
Create Method / Edit Method Screen
The Add Method Step window will appear.
The Step Types available in this screen are Treatment, Delay, Prompt and Repeat.
Add Step
Pull-down list of
methods
Edit the selected method
Create a new method
Basic
Settings

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Choosing Treatment allows editing of the four acoustic parameters:
a
Add a Treatment
Treatment parameters can be chosen either by sliding a control left or right, by clicking on
up/down arrows in the value box or by directly editing the Value box. The treatment parameters
are:
Peak Incident Power (PIP)
This is a measure of the instantaneous ultrasonic power applied to the sample. The range is 2.5
to 500 Watts.
Duty Factor
During treatments, acoustic power is “burst” or turned on and off according to Duty Factor and
Cycles per Burst. The duty factor is the percentage of time that the ultrasound signal is applied
to the sample. The minimum value is 0.1% and the maximum is 50.0%
Average Incident Power applied to a sample will be approximately the Peak Power multiplied by
the Duty Factor. For example, if Peak Incident Power is set to 100 Watts and Duty Factor is set
to 15%, the Average Incident Power will be approximately 15 Watts, but will vary with sample
tube type and water level and temperature.
In the S220 instrument, the maximum average power is 100 Watts. In the S220x instrument, the
maximum average power is 250 Watts, allowing application of greater average power.
Cycles per Burst
The ultrasound is delivered in sinusoidal bursts of ultrasonic energy. Cycles per burst are the
number of cycles of ultrasonic energy to deliver during the on portion of the duty cycle.
Duration
This parameter defines the amount of time, in seconds, that the sample is under a treatment.
Step Type

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Choosing a Delay step introduces a fixed time delay into a method. The user can set the sample
to settle between two different treatment steps. The delay is specified in seconds.
Add a Delay Step
Choosing Prompt will cause a specified message to display on the screen and pauses execution
of the method until the operator clicks OK or Cancel.
Add a Prompt Step
By choosing a Repeat step, the user can create a group and a number of repeat iterations.
Steps may then be moved into this group (see Available Functions below).
Add a Repeat Step

S220(x) User Manual Page 20
When method parameters for the step have been defined, click on OK to save changes and
return to Basic Settings.
4.2.3 From the Basic Settings Tab, once any step exists, general operation buttons are
available for the following functions:
Add Step Allows the user to add a step to the end of the method.
Insert Step Allows the user to select a step and insert a new step either before or
after the selected step.
Edit Step Allows the user to edit the selected step.
Remove Allows the user to remove the selected step.
Remove All Allows the user to remove all the steps. Before the remove, a
confirmation window will appear asking the user if the user wants to
remove all the steps.
Duplicate Allows the user to duplicate a selected step.
Group Steps Allows the user to create a group with one or more steps. A group may
be executed once, or repeated multiple times. A “Create Step Group”
window will appear allowing you to choose the number repeats.
Move Up Allows the user to move the selected step up one level. If the selected step is
already on the top of the list, it has no effect on the method. If the selected step is
in a group, a confirmation window will appear asking if the user wants to move
the step out of the group. This action may also be used to move a step into a
group.
Move Down Allows the user to move the selected step down one level. If the selected step is
already on the bottom of the list, it has no effect on the method. If the selected
step is in a group, a confirmation window will appear asking if the user wants to
move the step out of the group. This action may also be used to move a step
into a group.
Select All Allows the user to select all steps created.
When a group is created, you have the choice of running this
group of treatment once, or to repeat it multiple times. To
repeat, simply choose Repeat from the Group/Repeat pull-
down. The default repeat is 2. Double click on “Begin” to
bring up an Iterations screen. Up to 1000 repeats may be
programmed.
4.2.4 Temperature limits are set by editing the
Temperature Range values in the Basic Settings
Screen. Values may be set as low as 0.5°C and as high as 55.0°C.
4.2.5 If User Authentication is enabled, a method may be locked against editing by other
users. See “Operation with User Authentication” for more details.