Covaris S220 User manual

For research use only
S220 Series
USER MANUAL
Instrument and software developed for
high performance sample preparation
using adaptive focused acoustic energy
Product Name: S220-Series
Model Numbers: S220, S220x, S220R
Software: SonoLab 7

S220(x) User Manual Page 2
Trademarks
Patented or patent pending and registered or registration-pending trademarks of Covaris are protected.
Registered names and trademarks used in this document, even when not specifically marked as such, are not to be
considered unprotected by law.
WCS and AFA are either registered trademarks or trademarks of Covaris, Inc. in the United States and/or other
countries.
Clorox is a registered trademark of The Clorox Company.
Teflon is a registered trademark of The DuPont Company.
Information subject to change without notice
For Research Use Only
Not for use in diagnostic procedures
Publication P/N 010122
Revision I, May 2017
Product covered by Patent No. US6, 719,449, US6, 948,843, and other pending applications
Copyright © 2017 Covaris, all rights reserved.
Covaris, Inc.
14 Gill St, Unit H
Woburn, Massachusetts
01801-1721 USA Printed in U.S.A.
UNIVERSAL PRECAUTIONS
Universal Precautions should be followed on all specimen samples, regardless of whether a sample is known to contain
an infectious agent. Laboratories handling specimen samples are advised to comply with applicable parts of the following
governmental and clinical standards, or their equivalent in the country of use:
Centers for Disease Control (CDC), Universal Precautions for Prevention of Transmission of HIV and Other
Bloodborne Infections, published 1987, updated 1996
Clinical and Laboratory Standards Institute (CLSI), GP17-A2 Clinical Laboratory Safety; Approved Guideline -
Second Edition, published 2004, ISBN 1-56238-530-5
Clinical and Laboratory Standards Institute (CLSI), M29-A3 Protection of Laboratory Workers from
Occupationally Acquired Infections; Approved Guideline, Third Edition, published 2005, ISBN 1-56238-5674
Occupational Safety and Health Administration (OSHA), 29 CFR 1910.1030 Bloodborne Pathogens
International Standards Organization (ISO) 15190:2003, Medical Laboratories –Requirements for Safety

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Warnings
For safety of operating personnel:
Make sure that the equipment is properly grounded. DO NOT operate if it is not properly grounded.
The unit is equipped with a power plug appropriate for the destination country. DO NOT, under any circumstances,
remove the grounding prong from the power cord.
Do not run an acoustic treatment with the Acoustic Assembly and sample cover in the UP position or without a water
bath - the acoustic system will not work.
If there is any indication that the Safety System is not functioning properly, DO NOT operate the equipment and
contact Covaris immediately.
If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment
may be impaired.
To prevent damage to the equipment:
The instruments are designed to operate in ambient laboratory conditions e.g., 19ºC to 25ºC (66ºF to 77ºF). DO
NOT operate the instrument in a cold room environment; the system is designed to operate with a water bath and
re-circulating heater/chiller apparatus to control sample temperature.
NEVER run a method without a water bath; this could damage the transducer. The instrument is equipped with a
water level sensor to protect the transducer and degassing pump. The system will not allow the degassing pump to
operate or the acoustic treatment to start unless an adequate volume of water is detected.
Distilled or deionized water should be used to fill the water bath.
Unless a Water Conditioning System (WCS) is employed, empty the water bath and wipe it dry EVERY day with a
lint-free cloth. DO NOT leave water in the tank for an extended time as there is no water filtration or water cleaning
system within the apparatus (unless WCS is employed).
Do not employ isopropyl alcohol, ammonia-based or abrasive cleaners on the water tank, as these will damage the
acrylic surfaces.
Establish a standard of operation and periodically test equipment, as described in Maintenance (see section 6.0) of
this manual.
Loading third party software onto the computer may interfere with system operation. Please consult with Covaris.

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Warranty
When used in accordance with written instruction and under normal operating conditions, the Covaris instruments
are guaranteed to be free of defects in MATERIAL and WORKMANSHIP for one (1) year from the date of original
delivery by an authorized representative. Any component which proves defective during the stated period will be
repaired free of charge or replaced at the sole discretion of Covaris, F.O.B., Woburn, Massachusetts, U.S.A.
provided the defective component is returned properly packaged with all transportation charges prepaid. The
customer is expected to perform basic diagnostics and component replacement with telephone support from Covaris
personnel. If Covaris personnel are required to perform on-site repair, all travel related costs are paid by the
customer. A limited warranty as specified may apply to certain components of the equipment.
Warranty Exceptions
This warranty is void if failure of the software or hardware has resulted from accidents, abuse, improper
maintenance, or repair, or misapplication by the customer. It is also void if damage is caused by any unauthorized
attachments or if modifications are made to the equipment. Removing or tampering with the Safety Enclosure will
void the warranty, and the customer will assume all liabilities.
This warranty is limited to the original purchaser and is not transferable.
The software will perform according to the accompanying written materials and the medium on which the software is
delivered is free of defects in materials under normal use and service. The warranty is void if damage has resulted
from third party software not intended for use with the system.
The high power focused transducer is designed to give maximal mechanical energy output in water. Permanent
damage to the transducer and electronic circuits could result if the transducer is operated without water. Operation
of the system without water in the water bath voids the warranty.
CONTACT COVARIS, INC. SHOULD YOU HAVE ANY QUESTIONS CONCERNING EQUIPMENT
Warranty Services
The purchased equipment is covered by a twelve (12) month warranty which includes all the service and support
necessary so that the customer can operate the equipment successfully. Extended warranties are available at the
end of the original 12 month warranty period.
Services included with the original purchase of the system are:
Technical Support –On-going assistance with the operation or application of the equipment and/or troubleshooting
is provided via:
Telephone [phone]) during the hours of 9:00am to 5:00pm, Monday through Friday,
oUnited States, Eastern Standard Time (EST)
oGreenwich Mean Time (GMT-05:00)
E-mail queries to [email protected]
Parts Replacement –Replacement of parts (excluding consumables) from normal operation of equipment are
provided on a priority basis. All labor and shipping charges are included. Failure due to accident, abuse, or
improper operation is not covered.
An Operator’s Manual is provided with the equipment. This manual includes sections on the operating
instructions, maintenance guidelines, and troubleshooting tips.

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TABLE OF CONTENTS
1.0 INTRODUCTION................................................................................................................................6
1.1 Overview of the User Manual.........................................................................................................6
1.2 The S220 and the Covaris Process ...............................................................................................6
2.0 INSTALLATION AND SETUP............................................................................................................7
2.1 Assembly........................................................................................................................................7
2.2 S220 Series Installation .................................................................................................................8
3.0 GETTING STARTED .......................................................................................................................10
3.1 Fill the Water Bath........................................................................................................................10
3.2 Start the System...........................................................................................................................10
3.3 The SonoLab Run Screen - Running a Method...........................................................................11
3.4 System Shutdown ........................................................................................................................14
3.5 Temperature Control....................................................................................................................15
3.6 Degas System..............................................................................................................................15
4.0 SonoLab™ APPLICATION SOFTWARE.........................................................................................16
4.1 Run Screen ..................................................................................................................................16
4.2 Create and Edit Methods .............................................................................................................17
4.3 Temperature Tab..........................................................................................................................21
4.4 Saving Methods in Folders...........................................................................................................22
4.5 History Tab...................................................................................................................................23
4.6 Setup Screen................................................................................................................................23
4.7 Maintenance Screen ....................................................................................................................28
4.8 About Tab.....................................................................................................................................29
5.0 SYSTEM SPECIFICATIONS ...........................................................................................................30
6.0 MAINTENANCE...............................................................................................................................31
6.1 Cooling Air Intake.........................................................................................................................31
6.2 Water Bath ...................................................................................................................................31
6.3 Transducer...................................................................................................................................31
6.4 Safety System..............................................................................................................................31
6.5 Degassing System .......................................................................................................................31
6.6 Cleaning the System....................................................................................................................31
7.0 TROUBLESHOOTING.....................................................................................................................32
Appendix A: Suggested S220 Series IQ / OQ / PQ ....................................................................................34
Appendix B: S Series Site or Transport Preparation...................................................................................40
Appendix C: Installing SonoLab Software, Firmware and Instrument Drivers ............................................41
Appendix D: Recirculating Chiller................................................................................................................46

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1.0 INTRODUCTION
1.1 Overview of the User Manual
This manual contains operation and maintenance instructions for the Covaris S220 Series Focused-
ultrasonicators (S220, S220x or S220R). In this document, “S220” generally refers to any of these
S220 Series of instruments. This document contains information essential to the proper use and
care of the equipment.
Should any unforeseen problems occur with the normal operation of the equipment, contact Covaris
Technical Support immediately.
The following definitions apply in this manual:
NOTE: Inconvenience if disregarded.
CAUTION: Equipment damage may occur.
WARNING: Personal injury may occur.
There is also a PDF version of this manual, typically available on the computer desktop or
downloadable from the Covaris website. Open the manual by double clicking the user manual icon.
1.2 The S220 and the Covaris Process
The S220 Focused-ultrasonicator is based on Covaris Adaptive Focused Acoustics (AFA)
technology. This instrument is designed to provide researchers an integrated stand-alone tool to be
used in a laboratory environment. The instrument is ideal for those who work with tissue
homogenization, cell lysis, DNA or chromatin shearing, and other sample preparation steps.
The system is comprised of the Treatment System which delivers the acoustic energy to the sample,
the Safety System which protects users from inadvertently contacting the high intensity acoustic
energy, and the Computer and Application Software which provides users an interface to control the
device.
The Covaris Process uses adaptive focused acoustic energy to precisely control cavitation and
acoustic streaming within the sample treatment vessel in a non-contact, isothermal fashion. Focused
acoustic energy is generated and delivered to individual samples. The mechanical energy imparted
on the sample results in a controlled series of compression and rarefaction events.

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2.0 INSTALLATION AND SETUP
2.1 Assembly
The following items should be found in the package of the Covaris S220 instrument:
Quick Start User Guide
User Manual
Notebook Computer with AC Adapter
Cables
oUSB cable
oS220 power cord
oComputer AC Adaptor power cord
Accessories
oChiller Hose Kit
oSample Tube Holders (may vary according to application)
oS220 Water Tank
oS220 Main Unit
Please note that the SonoLab software installer, if required, is available from the Covaris website or
by contacting Covaris technical support.
The S220 Focused-ultrasonicator

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2.2 S220 Series Installation
2.2.1 Unpack the S220 and place it on a table or bench, with space to accommodate the
instrument and the laptop. The recirculating chiller may be placed on the floor to save
bench space.
2.2.2 Please refer to the rear panel of the S220 and identify the following features:
The Rear Panel of the S220 System
2.2.3 Plug the S220 into a power source using the enclosed line cord. The S220 is a
“universal input” device and automatically employs any worldwide line voltage or
frequency (100 to 240 volts AC, 50 or 60 Hz). Leave the instrument power and
notebook power switched OFF at this time.
2.2.4 Plug one end of the USB cable into the USB Port, and the other end into any USB port
on the notebook computer.
2.2.5 Connect the computer to a power source. Connect the mouse or other accessories to
the computer.
USB Port
Expansion Port
Ventilation Fan
Product Safety Label
Serial Tag,
Regulatory and
Line Power Label
AC Line (Mains) Input
Chiller Connections

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2.2.6 Connect the two hoses provided for coolant flow from the chiller. The connections are
bi-directional. (There is no defined IN or OUT.)
2.2.7 Connect the hoses to the chiller. Please refer to Appendix D and your chiller operation
manual for details on chiller connection and operation.
2.2.8 Position the S220 and computer in your work space as desired.
CAUTION: When positioning the system, please keep the fan and rear panel vents
free of obstructions that may block the flow of air.
CAUTION: To prevent chiller damage, use of a 20% glycol solution in the circulating
chiller fluid is strongly recommended. Please refer to the chiller’s user manual.
2.2.9 Unpack the Water Tank.
2.2.10 At the front of the S220, lift the Acoustic Assembly, slide the Tank into the frame as
shown, and lower the Acoustic Assembly. The WATER LEVEL label should face
forward.
The S220 Acoustic Assembly and Water Tank
2.2.11 If a Water Conditioning System (WCS) will be employed with the S220 system, please
refer to the WCS User Manual for further instructions. The WCS removes biological
growth and particulates from the water, allowing bath water to remain in place for up to
one month.
2.2.12 The S220 system is now ready to be started.
Safety Cover
Acoustic Assembly
Water Tank

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3.0 GETTING STARTED
3.1 Fill the Water Bath
The S220 transducer must be immersed in a water bath to transmit high-
frequency, focused acoustic energy to the sample tube. For best performance,
employ only clean, distilled or deionized water in the bath.
Lift the Acoustic Assembly, remove the tank and fill to the level specified in the
protocol to be used. Typical DNA Shearing protocols call for water level 12.
Avoid filling higher than 15, as the tank may overflow. Employ the markings on
the FILL side of the gauge. The tank holds approximately 0.5 gallons (1.6 liters).
Once the tank is in place and the Acoustic Assembly is lowered into the bath, the
water level should be read from the RUN side of the label.
3.2 Start the System
Turn S220 power ON using the red power switch located on the instrument. When
power is ON, this switch will illuminate and the ventilation fans in the rear panel will
run.
Also start the external chiller, programming its set point to a few degrees below the desired S220
bath temperature. Be sure to employ a 20% glycol solution inside the chiller to prevent freezing. Do
not use undiluted glycol solution.
Start the system computer. The computer employs a Windows®Operating System (XP, 7 or 8).
SonoLab 7 is an application used to control a Covaris Focused-ultrasonicator for processing a
biological or chemical sample. It is designed to create, edit, and run a processing program called a
method. It has provisions to run, pause or stop a method and view execution histories which contain
a record of key instrument parameter values during a run. It also contains editors for customizing
system settings and performing maintenance functions.
SonoLab 7 is installed on the computer purchased with the S220 instrument. No further
installation or configuration is required.
Find the SonoLab icon on the desktop:
If the icon is not visible, or if SonoLab 7 is not installed, please refer to Appendix C or contact
Covaris for installation instructions.
Double-click on the icon to start SonoLab. An animated splash screen will appear: