covetrus 801854 User manual

1
Microscope binocular (2)801854
User manual

2
INTRODUCTION
Thank you for purchasing the Covetrus biological microscope for routine investigation
The Covetrus microscopes are developed for use at schools and laboratories. Specific attention to
production methods resulted also in an excellent price/performance ratio
Please read this manual carefully before using this product to ensure correct and safe usage
• The contents of this manual are subject to change without notice
• The appearance of the actual product can differ from the models described in this manual
• Not all equipment mentioned in this manual has to be part of the set you have purchased
• All optics are anti-fungus treated and anti-reflection coated for maximum light throughput

3
Index
INTRODUCTION ............................................................................................................................ 2
Notes on handling and Safety ...................................................................................................... 4
Components of the microscope .................................................................................................. 5
Preparing the microscope for use ............................................................................................... 6
Assembling Steps ........................................................................................................................ 7
Operation ....................................................................................................................................... 8
Setting up the illumination: .......................................................................................................... 8
Place the specimen slide ............................................................................................................. 8
Focusing and slide protection ...................................................................................................... 8
Adjusting the focusing tension ..................................................................................................... 9
Eyepieces .................................................................................................................................... 9
1. The interpupillary distance ................................................................................................... 9
2. The correct eye point ........................................................................................................... 9
3. Adjusting the diopter ............................................................................................................ 9
Abbe condenser .......................................................................................................................... 9
The field (Köhler) diaphragm ..................................................................................................... 10
Adjusting the Aperture Diaphragm ............................................................................................ 10
Use of the S100x oil-immersion objective.................................................................................. 10
Safety device ............................................................................................................................. 11
Illumination ................................................................................................................................ 11
Maintenance and cleaning ......................................................................................................... 11
Cleaning the optics .................................................................................................................... 11
Maintenance of the stand .......................................................................................................... 11
Changing the batteries of the microscope ................................................................................. 12
Replacing the fuse ..................................................................................................................... 12
Digital models and cameras ....................................................................................................... 12

4
Notes on handling and Safety
Handle with care
• This product is a high quality optical instrument. Delicate handling is required
• Avoid subjecting it to sudden shocks and impacts
• Impacts, even small ones, can affect the precision of the objective
Handling the LED
Note: Always disconnect the power cord from your microscope before handling the LED bulb and
power unit and allow the system approximately 35 minutes to cool down to avoid burns
• Never touch the LED with your bare hands
• Dirt or fingerprints will reduce the life span and can result in uneven illumination lowering the optical
performance
• Use only Covetrus original replacement LEDs
• Use of other products may cause malfunctions and will void warranty
• During use of the microscope, the power unit will get hot, never touch it while in operation and allow
the system approximately 35 minutes to cool down to avoid burns
Dirt on the lenses
• Dirt on or inside the optical components such as eyepieces, lenses, etc., affect the image quality of
your system negatively
• Always try to prevent your microscope from getting dirty by using the dust cover, prevent leaving
fingerprints on the lenses and clean the outer surface of the lens regularly
• Cleaning optical components is a delicate matter. Please read the cleaning instructions in this manual
carefully
Environment, storage and use
• This product is a precision instrument and it should be used in a proper environment for optimal use
• Install your product indoors on a stable, vibration free and level surface
• Do not place the product in direct sunlight
• The ambient temperature should be between 5 to +40°C and humidity is maximum 80% at 31 degrees
decreasing linearly to 50% at 40 degrees. Although the system is anti-mold treated, installing this
product in a hot, humid location may still result in the formation of mold or condensation on lenses,
impairing performance or causing malfunctions
• Never turn the right and left focus knobs in opposite directions at the same time or turn the coarse
focus knob past its farthest point, this will damage the microscope
• Never use undue force when turning the knobs
• Make sure that the microscope system can dissipate its heat
• Keep the microscope approximately 15cm free from walls and obstructions
• Never turn the microscope on when the dust cover is in place or when items are placed on the
microscope
• Keep flammable fluids, fabric etc. well out of the way
Disconnect power
• Always disconnect your microscope from power before doing any maintenance, cleaning, assembling
or replacing LEDs to prevent electric shocks

5
Prevent contact with water and other fluids
• Never allow water or other fluids to come in contact your microscope, this can cause short circuiting
your device, causing malfunction and damage on your system
Moving and assembling
• The microscope is a relatively heavy system, consider this when moving and installing the system
• Always lift the microscope by holding the main body and base of the microscope
• Never lift or move the microscope by its focusing knobs, stage or head
• When needed, move the microscope with two persons instead of one
The S40x and S100x objectives are equipped with a spring mount, to prevent damage to the front lens and
the slide. The Numeric Aperture - N.A. – of the objective is an indication for the resolving power of the
objective. The total magnification can be calculated by multiplying the magnification of the eyepiece with the
magnification of the objective. The magnifications are displayed in the table below:
Eyepiece
Objective
Magnification
10x
4x
40x
10x
10x
100x
10x
40x
400x
10x
60x
600x
10x
100x
1000x

6
Components of the microscope
The names of the several parts are listed below and are indicated:
C Stand arm
K Tube (mono/bino/trino 360° rotatable)
D Safety device
L Revolving nosepiece for 4 objectives
E Tension adjustment
M Objectives
F Coaxial coarse-and fine adjustment
N Object stage (mechanical stage or clips)
G On/Off switch (not visible)
O Condenser with irisdiaphragm + filterholder
H Light intensity control
P Coaxial stage controls
I Eyepiece(s)
Q Lamp housing
J Dioptric adjustment (bino/trino type)
Preparing the microscope for use
Carefully remove the items from its packaging and place them on a flat, firm surface. Please do not expose
the microscope to direct sun light, high temperatures, damp, dust or acute shake. Make sure the table or
surface is flat and horizontal

7
When moving the microscope, use the left hand to hold the transport handle and hold the base of the
microscope with the right hand
Hold the microscope at the top of the
stand arm when it should be moved
Holding the microscope by its stage or focusing knob will
damage the microscope
Caution!
If the bacterial solution or water splatters over
the stage, objective or head, pull out the power cord
immediately and dry the microscope
Assembling Steps
Covetrus always try to keep the number of assembly steps for their customers as low as possible but in some
cases there are some steps to be taken. The steps mentioned below are often not necessary but described
for your convenience nonetheless
Mounting the objectives
1. Rotate the coarse focusing knob to lower the stage to its lowest position
2. Install the objectives into the objective nosepiece from the lowest magnification to the highest in a
clockwise direction from the rear side of the microscope. When using the microscope, start using the low
magnification objective (4X or 10X) to search for specimen and focus, and then continue with high
magnification objective to observe
Placing the eyepieces
1. Remove the cover of eyepiece tube
2. Insert the eyepiece into the eyepiece tube
3. Lock the eyepieces with a hexagon screw
Locking the eyepieces
To lock the eyepieces in binocular models please find the screw as
indicated in picture (A). Please note that location can be slightly
rotated from model to model.

8
The eyeshades (optional)
The eyepieces can be equipped with optional rubber eyeshades. This prevents damage to the lens, and stray
light. The eyeshade can simply be slipped over the eyepiece
Connecting the power cord
The microscope supports a wide range of operating voltages: from 100 to 240V. Please use a grounded power
connection
1. Make sure the power switch is off before connecting
2. Insert the connector of power cord into the microscope power socket, and make sure it connects well
3. Insert the other connector into the mains socket, and make sure it connects well
Do not bend or twist the power cord,
it will be damaged. Use the power cord that is supplied by Covetrus. If
it’s lost or damaged, choose one with the same specifications
Operation
Setting up the illumination:
For optimal contrast and resolution one should follow the below procedure:
1. Place a specimen on the object stage and focus using the 4x objective, with a fully opened iris diaphragm
2. Turn light intensity to lowest position, then look through the eyepiece(s) and turn up to the comfortable
light intensity level
3. Turn the condenser in the highest position
4. Close the iris diaphragm, until it is just visible on the edge of the field of view
The microscope is properly set for use with the 4x objective. For each other magnification in bright field use
this procedure should be repeated to ensure the best balance between contrast and resolution
Caution:
The maximum light intensity when using the 4x and 10x can damage the eyes!
Place the specimen slide
Place slide into the clamp of the mechanical stage (G) and can be carefully moved into X- and Y- directions
1. Push the arm of the specimen holder backwards
2. Release the arm slowly clamping the slide with the cover glass facing up
3. Rotating the X and Y-axis knob will move the specimen to the center for alignment with the center of the
objective
Focusing and slide protection
1. Select the 4x objective and make sure that it is placed correctly in the optical path
2. Rotate the position screw to top, observe the right eyepiece with your right eye. Rotate the coarse
focusing knob until the image appears
3. Rotate the fine focusing knob to sharpen the image
4. When you perform focusing with a S100x objective, you need to lock the slide protection handle. The
slide protection handle protects the slide by limiting the travel range of the mechanical stage. This way
the objectives will not touch or break your slides

9
Adjusting the focusing tension
The tension of the focusing knobs can be adjusted.
You can set it from light to heavy according your
own preference. Please note that when the
specimen leaves the focus plane after focusing or
the stage declines out of its own, then you need to
adjust the tension
To tighten the focusing knob (more heavy), rotate
the tension adjustment ring counter-clockwise; to
loosen it, please turn it in the clockwise direction
Eyepieces
Using a binocular (or trinocular) tube is less tiring
for the eyes than a monocular tube. In order to
obtain a smooth “compound” image, we
recommend you to go through the below steps
1. The interpupillary distance
The correct interpupillary distance is reached when one round image is seen in the field of view (see image
below). This distance can be set by either pulling the tubes towards each other or pulling them away from
each other. This distance is different for each observer and thus should be set individually. When more users
are working with the microscope it is recommended to remember your interpupillary distance for a quick set
up during new microscopy sessions
2. The correct eye point
The eye point is the distance from the eyepiece to the user’s pupil. To obtain the correct eye point, move the
eyes towards the eyepieces until a sharp image is reached at a full field of view
3. Adjusting the diopter
• Set diopter adjustment ring to zero.
• Close the left eye and focus the right tube by adjusting the coarse- and fine adjustment knobs.
• Close the right eye and focus the left tube with the diopter adjustment ring
This procedure should be followed by each individual user. When more users are working on the same
microscope it is recommended to remember your own diopter setting for a quick set up during new
microscopy sessions
Abbe condenser
Beneath the object stage an Abbe condenser N.A. 12.5 is mounted. The condenser can be adjusted in height
by moving the rack and pinion knob beneath the mechanical stage. By adjusting the condenser you can focus
Focusing tension
Field of view before
adjustment
Field of view after
adjustment

10
the light on the specimen for an optimized contrast. The condenser is factory pre-centered. If needed, the
following procedure can be followed to center the condenser
1. Move the condenser to its highest position
2. Select the 10x objective and place it into the light path and focus the specimen
3. Rotate the field diaphragm adjustment ring to put the field diaphragm to the smallest position
4. Adjust the condenser to the point where the image is the sharpest
5. Adjusting the center adjustment screw and put the image to the center of the field of view
6. Open the field diaphragm gradually
7. The condenser is centered correctly if the image remains in the center when you open the field
diaphragm and inscribed to the field of view
The field (Köhler) diaphragm
By limiting the diameter of the beam entering the condenser, the field diaphragm can prevent other light and
increase the image contrast. When the image is just on the edge of the field of view, the objective can show
the best performance and obtain the clearest image. The diaphragm is factory pre-centered
Adjusting the Aperture Diaphragm
1. The aperture diaphragm is used to select the numerical aperture of the illumination. When the N.A. of
illumination matches with the N.A. of the objective, you get the highest possible resolution, depth of field
and contrast
2. When contrast is low, rotate the diaphragm adjustment ring to 70%-80% of the N.A. of objective this will
improve the contrast of the image. The diaphragm is factory pre-centered
Use of the S100x oil-immersion objective
All Covetrus microscopes are equipped with an S100x N.A. 1.25 oil immersion objective. Please follow the
below instructions on how to use this objective:
1. Remove the dust protection cap from the revolving nosepiece to mount the S100x objective
2. Focus the image with the S40x objective
3. Lock the slide protection handle
4. Turn the revolving nosepiece so the S100x objective almost reaches the click-stop
5. Put a small drop of immersion oil on the center of the slide (always use Covetrus Immersion oil)
6. Now turn the S100x objective so that you feel the click stop
7. The front lens is in contact with the immersion oil
8. Look through the eyepiece and focus the image with the fine adjustment knobs
9. The distance between the lens of the objective and the slide is very small!
10. In case there are small bubbles visible, turn the S100x objective a couple of times from left to right so
that the front of the objective moves in the oil and the bubbles will disappear
11. After using the S100x objective, loosen the slide protection handle and turn the table with the course
adjustment knobs downwards until the front lens doesn’t touch the oil any longer. Clean the front
lens of the S100x objective

11
12. Always clean the front lens of the S100x objective with a piece of lens paper that is moistened with a
drop of isopropanol. We recommend using Covetrus lens paper and isopropanol
13. Clean the slide after use as well
Caution
• Never put a drop of xylol or alcohol directly on the lens of the objective. It could enter the objective and
dissolve the glue that holds the lenses!
• Avoid oil contact with any of the other objectives!
Safety device
To prevent damage to the objective lens, or breaking the slide, all types are equipped with a pre-fixed safety
device
It is recommended to use slides of 1.0 – 1.2 mm thickness in combination with cover glasses of 0.13 mm or
0.17 mm thickness
Illumination
The LED illumination of the microscope is equipped with rechargeable batteries.
The length of use after charging is about 60 hours. The full charging time is about 10 hours. At First use the
batteries have to be fully charged. Connect the cable to the mains socket
The illumination has the following specifications:
• LED : 1W, 300 mA
• Charger : Primary AC 100 - 240 Volt-50Hz
• Batteries : 3 NiMh, AA type, 1.2 Volt 1600 - 2600 mA
Maintenance and cleaning
Always place the dust cover over your microscope after use. Always keep the eyepiece and objectives
mounted on the microscope to avoid dust entering the instrument
Cleaning the optics
When the eyepiece lens or front lens of the 10x or S40x objective are dirty, they can be cleaned by wiping a
piece of lens paper over the surface (circular movements). When this does not help put a drop of alcohol on
the lens paper and wipe it. Never put xylol or alcohol directly on the lens! Please note that Covetrus offers a
special microscope cleaning kit
It is not necessary – and not recommended – to clean the lens surfaces at the inner side of the objectives.
Sometimes dust can be removed with high pressured air. There will never be dust in the objectives if the
objectives are not removed from the revolving nosepiece
Caution
Cleaning cloths containing plastic fibers can damage the coating of the lenses!
Maintenance of the stand
Dust can be removed with a brush. In case the stand or table is really dirty then you can clean the surface
with a non-aggressive cleaning product
All moving parts like the height adjustment or the coaxial course and fine adjustment contain ball bearings
that are not dust sensitive. With a drop of sewing-machine oil you can lubricate the bearing

12
Changing the batteries of the microscope
Caution
: Always remove the power cable from the mains supply!
Remove the small lid out of the bottom cover of the microscope
• Place the batteries and put the lid back into its place
Replacing the fuse
To change the fuse, please follow these procedures:
• Remove the power cord from the back of the microscope
• Locate the fuse compartment, which has a Fuse image. It is typically located beneath
the power connector
• Remove the fuse compartment. To do so, insert a flat head screwdriver between the
metal power tines and gently pry the fuse compartment loose with a slight down and
out motion
• Insert the new fuse into the compartment, and replace the fuse compartment back
to where it was originally
• Power up the microscope and test
Note
: Fuse may blow. In most cases, replacing the fuse with the correct voltage will
resolve the issue. However, should you encounter a blown fuse frequently, please
contact your distributor for further assistance
Fuse specification: 250V 1A
Digital models and cameras
Digital cameras are designed to be used on the photo port of
the microscope head. It is also possible to use the digital
camera in combination with a binocular head. To use the
camera on a binocular head, you can simple remove the
eyepiece[1] and then place the camera with mounted c-
mount adapter into the eyepiece tube[2]. Focus the digital
image with the coarse and fine controls of the microscope
Take an easy-to-view specimen and focus the image through
the microscope’s eyepieces. For focusing the camera, slowly
move tube (A) up and down while watching at the screen till
the camera view is in focus
Follow the manual that comes with the camera for camera
operation
Binocular head with camera replacing
the original eyepiece