Cypress Diagnostics CYANSmart User manual

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CYANSmart
Service Manual
For a clear and precise diagnose
CY009
English

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [e-mail]
CY009 – CYANSmart – Service Manual
CYANSmart Service Service Manual, version 20191121 (8.0) Page 1
TABLE OF CONTENTS
1 INTRODUCTION .................................................................................................................................................. 3
1.1 SYSTEM DESCRIPTION .................................................................................................................................... 3
1.2 PRINCIPLE OF MEASUREMENT ....................................................................................................................... 3
1.3 TECHNICAL AND OPERATIVE SPECIFICATIONS ............................................................................................... 4
2 INSTALLATION ..................................................................................................................................................... 5
3 MAINTENANCE .................................................................................................................................................... 6
3.1 PUNCTUAL MAINTENANCE ........................................................................................................................... 6
3.2 ROUTINE MAINTENANCE ............................................................................................................................... 6
3.2.1 Washing 6
3.2.2 Cleaning 7
3.2.3 Verification 7
3.3 REPLACEMENT OF SPECIFIC PARTS ................................................................................................................ 8
3.3.1 External cleaning 8
3.3.2 Internal washing 8
3.3.3 Replacement schedule 8
4 TECHNICAL CONTROL ...................................................................................................................................... 9
4.1 GENERAL DESCRIPTION AND BLOCK DIAGRAM ............................................................................................. 9
4.2 MAIN CONTROL BOARD............................................................................................................................... 10
4.2.1 Connections on the mainboard 10
4.2.2 Voltage check points on the mainboard 11
4.2.3 Verification of the voltage to the mainboard 11
4.2.4 Replacement of the mainboard 12
4.3 OPTICAL SYSTEM ......................................................................................................................................... 12
4.3.1 Lamp 13
4.3.2 Filter wheel 13
4.3.3 Flow cell 13
4.3.4 Pre-amplifier board 14
4.3.5 Verification of the optical system 14
4.3.6 Cleaning and/or replacement of the flowcell 16
4.3.7 Verification of the filter wheel home sensor 17
4.3.8 Cleaning and/or replacement of the filter wheel home sensor 18
4.3.9 Verification of the filter wheel motor 19
4.3.10 Replacement of the filter wheel motor 19
4.3.11 Cleaning and/or replacement of filters 21
4.3.12 Verification of the voltage to the lamp 22
4.3.13 Replacement of the lamp 23
4.3.14 Replacement of the pre-amplifier board 26
4.3.15 Replacement of the optical system 26
4.4 HYDRAULIC AND ASPIRATION SYSTEM ........................................................................................................ 28
4.4.1 Verification of the aspiration and hydraulic system by pump calibration 28
4.4.2 Replacement of tubes in the hydraulic circuit 30
4.4.3 Replacement of peristaltic pump tube 31
4.4.4 Replacement of peristaltic pump rubbers 32
4.4.5 Cleaning and lubrication of the pump motor with rotor 32
4.4.6 Verification of the pump motor 33
4.4.7 Replacement of the pump motor with rotor 34
4.4.8 Verification of the aspiration switch 34
4.4.10 Replacement of the aspiration switch 35
4.4.11 Aspirate format 35

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4.5 INCUBATOR BLOCK ...................................................................................................................................... 36
4.6 TEMPERATURE ............................................................................................................................................. 36
4.6.1 Temp display 36
4.6.2 Temperature calibration 37
4.6.3 Cleaning and/or replacement of the fan of the optical system 39
4.6.4 Verification of the Peltier element 40
4.6.5 Replacement of the Peltier element 40
4.6.6 Verification of the heater element of the incubator 41
4.6.7 Replacement of the heater element of the incubator 42
Verification of the temperature sensor 43
4.6.8 43
4.6.9 Replacement of the temperature sensor 44
4.6.10 Verification of the voltage going to the heater elements on the mainboard 44
4.7 THERMAL CARBON PRINTER ........................................................................................................................ 45
4.7.1 Install or change the print paper 45
4.7.2 Verification of the voltage to the printer on the mainboard 46
4.7.3 Replacement of the printer 46
4.8 TOUCH SCREEN ............................................................................................................................................ 47
4.8.1 Touch screen calibration methods 47
4.8.2 Adjustment of the screen brightness 48
4.8.3 Verification of the voltage to the touch screen on the mainboard 48
4.8.4 Replacement of the touch screen cable 48
4.8.5 Functional verification of the parts of the touch screen 49
4.8.6 Replacement of the touch screen 50
4.9 POWER SUPPLY ........................................................................................................................................... 51
4.9.1 General electricity and installation requirements 51
4.9.2 Replacements of fuses 52
4.9.3 Locating power problem 53
4.9.4 Verification of the fuses 54
4.9.5 Verification of the power plug connector and power switch 54
4.9.6 Verification of the power supply 56
4.9.7 Cleaning and/or replacement of the fan for power assembly 57
5 VALIDATION ....................................................................................................................................................... 58
5.1 PREPARATION OF A DILUTION SERIES .......................................................................................................... 58
5.1.1 Ammonium colbalt (II) sulfate hexahydrate (powder) 58
5.1.2 Potassium dichromate (powder) 59
5.1.3 Magnesium Xylidyl Blue (liquid) 59
5.2 VERIFICATION OF PRECISION ....................................................................................................................... 59
5.3 VERIFICATION OF LINEARITY ........................................................................................................................ 60
5.4 VALUE 60
6 TROUBLESHOOTING ........................................................................................................................................ 61
6.1 OPERATIONAL ............................................................................................................................................. 61
6.2 TECHNICAL .................................................................................................................................................. 63
7 SAFETY INFORMATION ................................................................................................................................... 65
INSTALLATION FORM
MAINTENANCE FORM
REPAIR FORM
SPARE PARTS

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1 INTRODUCTION
1.1 SYSTEM DESCRIPTION
The CYANSmart is an easy to operate semi-automatic biochemical analyzer. It is a microcomputer-based
independent unit, controlling several systems: built-in thermal printer, touch screen, optical detection
system with flowcell, incubator and aspiration system.
1. Thermal printer
2. Touch screen
3. Optical system
4. Incubator block
5. Peristaltic pump
6. Aspiration “Push” button
7. Inlet tube
8. Rear panel
WARNING! Please read the service manual before opening the CYANSmart and performing problem
diagnosis or replacements.
BIOHAZARD: Wear personal protective equipment. Use universal precautions. See chapter 8. Safety
information for recommended precautions when working with biohazard materials.
1.2 PRINCIPLE OF MEASUREMENT
The CYANSmart is a semi-automated biochemistry analyzer. Samples are prepared manually outside the
instrument and after an incubation period (in- or outside the instrument) the samples are measured in the
flowcell and the analyzer calculates the result.
A halogen lamp emits light which passes through a filter selecting the required wavelength. The light then
passes through the sample in the flowcell. The sample will transmit or reflect fractions of the light. The
detector will measure how much light was reflected from/transmitted through the sample. This
measurement is then amplified and converted to a digital number for further calculations. These
calculations can be performed because there is a relationship between how much light is
reflected/transmitted and the concentration of the substances in a sample.
Lamp Filter Flowcell Photodiode detector
⑦
⑤
④
①
②
③
⑥
8

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1.3 TECHNICAL AND OPERATIVE SPECIFICATIONS
Optical system
Flowcell 32 µl, 10 mm light path
Min. reaction volume 500 µl per test
Light source Halogen lamp 6V / 10W
Photo detector Silicon based (range 300 – 900 nm)
Measurement range 0,000 – 3,500 Abs
Wavelength 340 – 620 nm
Wavelength selection Automatic via 7 interference filters: 340nm, 405nm, 492nm, 510 nm,
546nm, 578nm, 620nm
Flowcell
 25°C, 30°C, 37°C, ambient temperature (R.T.)
 Peltier element
 Thermostatic control: PID controlled, ± 0.5°C
Incubator
 20 places with 14 mm diameter
 37°C
 Thermostatic control: PID controlled, ± 1°C
Display
 Back-illuminated LCD
 Touch screen calibration possible
 800 x 480 pixels (screen size: 7.0 Inch, 155.3 x 88 mm)
Printer
 Automatic or on-demand printing
 Built-in thermal printer
 24 characters per line, prints graphs
Software
Data storage 180 test methods and 6000 sample test results
Methods Cypress methods pre-programmed at delivery
Languages English, French, Spanish
Quality control feature 2 controls programmable
Separate QC result menu with (printable) graphs
Calculation methods Absorbance, end point, two-point, kinetic, bichromatic
Calibration methods Factor, calibrator and multi-point calibration
Blank options Sample blank, reagent blank and distilled water
Incubation time 0 – 999 seconds
Reading time 3 – 999 seconds
Power supply
 AC 220 V ± 10 %, 50 Hz ± 2 % or AC 110 V ± 10 %, 60 Hz ± 2 %
 Automatic voltage and hertz switch
 Grounding required
 2 fuses T2AL250V
 Power: 200VA, reduced to 140 VA in standby mode
 Power save / Standby mode
Weight and dimensions
 Instrument: 9 kg, 435 x 415 x 200 mm (LxWxH)
 Packed in box: 11.5 kg, 520 x 430 x 260 mm (LxWxH)
Environmental requirements
 Ambient temperature: 15°C – 30°C
 Storage temperature: 5-50°C
 Relative humidity: 30 % - 70%
 Air pressure: 860 hPa – 1060 hPa

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
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2 INSTALLATION
The CYANSmart is an easy to operate instrument with integrated user-friendly software. No additional
computers or instruments are needed for good operations. In order to fully guarantee the performance of
the CYANSmart, it is extremely important that it is correctly installed.
If the installation, usage and maintenance directions are not followed correctly and/or safety indications are
not respected, Cypress Diagnostics cannot guarantee correct functioning of the instrument. Misuse can
compromise the safety of the operator and his surroundings and will void the warranty. Consumables are
not included in the warranty.
WARNING! Do NOT discard the carton box or protection material! If you need to ship the instrument (for
example for service), be sure to use the original packing materials. Other forms of commercially available
packing are not recommended and can void the warranty.
At installation, it is strongly recommend to fill in the installation form. It contains essential information and
also provides a short checklist of all installation requirements.
The serial number can be found on the back label. This is essential information in every communication
regarding the analyzer. The software version can be found in the System Settings – System Info.
Packing list:
Code Description Quantity
CY009 CYANSmart Analyzer 1
- User manual including application sheets 1
- CD with PC software (from P/D 2016/09 on) 1
CY004/9-S28 Waste tube 1
CY004/9-S44 Waste bottle 1
CY004/9-S22 Aspiration button 1
CY004/9-S49 Power cord 1
CY004/9-S48 Fuse T2AL250V 2
CY009-S11 Lamp with frame, cable and connector 1
CY009-S25 Pump tube 1
CY001-S01 Thermal print paper 5
CY004/9-S43 Dust cover 1
CY009-S56 USB cable (from P/D 2016/04 on) 1
For the detailed description of the installation, please check the user manual Chapter 2.
After turning off the analyzer, you need to wait at least 60 seconds before turning it on again!
Otherwise, the fuses will blow because of the residual current.
②

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3 MAINTENANCE
This chapter contains all routine operations concerning instrument maintenance. The procedures, listed
and described below, should be carefully followed in order to guarantee the manufacturers quality
specifications and the perfect working conditions of the instrument over time.
The CYANSmart requires three levels of maintenance:
 Punctual maintenance: at special moments such as at installation
 Routine maintenance: to maintain the level of precision day in day out
 Special maintenance: replacement of specific parts
Please consult the maintenance form for an overview of the different maintenance actions. In case of repair,
please register all undertaken actions in the repair form and sign it in order to keep track of the
interventions.
3.1 PUNCTUAL MAINTENANCE
The punctual maintenance should be effectuated at installation and before or after a long period of
inactivity. The following points of attention should be considered:
 Check the installation instructions.
 Check all tubes visually for leaking or squashing. Pay special attention to the peristaltic pump
tube. Replace if necessary.
 Removing dust from the instrument.
 Wash the flowcell with a detergent (CYAN Wash Solution CY001-WS or 5% Tween 20) and rinse
the flowcell with distilled water.
 Disinfect the flowcell with a 5% Hypochlorite solution and rinse with distilled water.
 Check the pump calibration.
 Check the AD auto zero and replace the lamp/filters if necessary.
 In case the instrument will not be used for a long period:
o Remove all liquid inside the instrument by aspirating air.
o Disconnect the pump tube.
3.2 ROUTINE MAINTENANCE
Frequency What Cleaning solution
Between methods Rinsing Distilled water
End of the day
Washing
Detergent + rinse with distilled water + rinse with air
Every week
Detergent + sodium hypochlorite (diluted 5 %) + rinse
with distilled water + rinse with air
End of the day (outside) Cleaning CYDIS surface/detergent + dust cover
Every 6 months (inside) Pressurized air (by service engineer)!
Every morning Verification AD auto zero
Every week Pump calibration
3.2.1 WASHING
i. Between tests
Between methods, always rinse the flowcell with distilled water.
It is not necessary to wash the instrument between different samples for the same method. However:
 In case of high risk of cross-over, you could pre-rinse the flowcell by aspirating distilled water, followed
by air (to avoid dilution) between the samples.
 In case of a strongly colored reagent, it might be necessary to pre-rinse the flowcell with (working)
reagent before aspirating the first measurement. Otherwise the first measurement could be
influenced by the distilled water used to zero the instrument.

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ii. Daily washing
At the end of each working day, wash the instrument with a neutral detergent (CYAN Washing Solution
CY001-WS or 5% Tween 20):
1. Position the detergent solution under the inlet pipe.
2. Press the “Tube Wash” button at least 10 times (=15 mL).
3. Repeat this operation using air at least once to make an air gap between the detergent and the distilled
water.
4. Repeat this operation using distilled water at least 10 times (=15 mL) to rinse the system.
5. Repeat this operation using air at least 10 times (=15 mL) to dry the hydraulic system and avoid
microbiological contamination.
iii. Weekly washing
After a week of work, wash first with 15 ml detergent (end of day procedure); and then disinfect the
hydraulic system (tubes and flowcell) with 5% sodium hypochlorite (bleach).
1. Position the hypochlorite solution under the inlet pipe.
2. Press the “Tube Wash” button at least 10 times (=15 mL).
3. Incubate the hypochlorite in the flowcell for about 10 minutes (no longer!).
4. Aspirate air (1x) and then rinse the instrument with distilled water (10x) and air (10x).
NOTES:
a. In case of air bubbles (visible during AD auto zero or perform test) rinse with diluted sodium
hypochlorite (5 %) then rinse with distilled water (10x), and with air (10x).
b. This diluted sodium hypochlorite is normally market available. Please use only good trademarks. Cheap
ones contain contaminating solutions.
c. Always dilute the sodium hypochlorite for 5%, never use it undiluted! This will damage the
instrument.
d. Do not use corrosive detergents to wash the instrument.
3.2.2 CLEANING
i. Daily cleaning
Please notice that the instrument, especially the optical system and the electrical circuit, are sensitive to
dust. Avoid using the instrument in dusty spaces. Cover the instrument when not in use and keep the
printer, pump and flowcell cover closed during use.
At the end of each working session, dampen a tissue with a non-abrasive detergent like CYDIS Surface (Cod.
CYDIS-S) or a 6% ESOFENOL solution and wipe the outside of the instrument.
ii. Every 6 months
It is recommended to clean the dust from inside the instrument at least every 6 months. To do so, remove
the cover and blow the dust off the instrument using pressurized air. Make sure the air is completely dry!
Open the filter cover and blow the dust off.
NOTES:
 The instrument cover should only be removed when the CYANSmart is unplugged.
 We strongly advise to leave this procedure to the technical assistance of the service engineer of your
distributor.
3.2.3 VERIFICATION
i. Daily
Perform an AD auto zero
ii. Weekly
Perform a pump calibration

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3.3 REPLACEMENT OF SPECIFIC PARTS
Before replacing any instrument part, repairing any defective item or performing any instrument
maintenance procedure(s), the operator or maintenance technician must carry out the below-described
decontamination procedure of the instrument part(s) involved in the operation(s).
3.3.1 EXTERNAL CLEANING
Use CYDIS Surface Cod. CYDIS-S or a 6% ESOFENOL solution (60 cc in one liter of distilled water). These are
antibacterial and antiviral substances.
 Spray the solution all over the instrument.
 Allow the solution to stand for approximately 30 minutes.
 Wipe the solution off the instrument using a sponge dampened with distilled water.
3.3.2 INTERNAL WASHING
Wash first with 15 ml detergent (end of day procedure); and then disinfect the hydraulic system (tubes and
flowcell) with 5% Hypochlorite (bleach). Make sure to incubate for 10 minutes (no longer) and then rinse
sufficiently with distilled water (procedure weekly washing).
3.3.3 REPLACEMENT SCHEDULE
Some spare parts should be replaced after a fixed period of time to ensure the good performance of the
analyzer. Please check the table below:
Spare part Replacement, when? Who?
Peristaltic pump tube Every six months User / Service engineer
All tubes of hydraulic
circuit
Every year or in case of leakage, obstruction or
strong coloration
Service engineer
Lamp Every year Service engineer
Amplifier board Every 2/3 years (together with lamp replacement) Service engineer
Peristaltic pump
rubbers
Every 2/3 years (together with pump tube
replacement)
Service engineer
Connectors Every 2/3 years (together with the replacements of
the tubes)
Service engineer
Flow cell In case of leakage, obstruction or strong coloration Service engineer

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4 TECHNICAL CONTROL
4.1 GENERAL DESCRIPTION AND BLOCK DIAGRAM
The main control board controls the overall operation of the instrument, including motor control,
temperature control and data management etc. It receives power from the power supply.
① Main control board
② Optical system
③ Peristaltic pump
④ Push button
⑤ Incubator
⑥ Fans
⑦ Power supply
The touch screen and the printer with separate board are connected to the mainboard but integrated in the
instrument cover.
Main Control Board
Printer
Optical system:
Filter motor & sensor
Lamp
Flow cell temp control
Photodetector
Touch screen
Pump Fans
USB
Incubator
Power supply
Push button
②
⑥
⑦
①
②
③
④
⑤
⑥

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4.2 MAIN CONTROL BOARD
4.2.1 CONNECTIONS ON THE MAINBOARD
The figure below shows the different connections. In case of problems, verify that the connection is correct
and firm. Try to disconnect and then reconnect.
1 Printer power (5V) 10 RS232 interface
2 Lamp power (3 or 6V) 11 Aspiration switch
3 Flowcell temperature sensor 12 Motor of filter wheel
4 Incubator temperature sensor 13 Motor of peristaltic pump
5 Pre-amplifier board 14 Heater of incubator
6 Printer data cable 15 Fan for power assembly*
7 Home sensor of filter wheel 16 Fan for optic system*
8 Touch screen 17 Heater (Peltier) of flowcell
9 USB interface 18 Power input 24V
*: In instruments from S/N BS6KMxxx on both fans are connected to connection n° 15 on the mainboard.
The following sections provide more explanation on the individual functions.
7
1
3
2
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18

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4.2.2 VOLTAGE CHECK POINTS ON THE MAINBOARD
The mainboard receives 24V from the power supply and will then regulate and provide the voltage
necessary for every other part. The figure below shows the different voltage check points.
More explanation about the test points can be found in the relevant chapter (verification of the voltage
to...).
1 Input power +24V 7 Artificial circuit -5V
2 Digit circuit +9V 8 Lamp +3/6V
3 LCD/USB 5V 9 Printer +5V
4 Peltier flowcell +15V 10 Power supply changeover -12V
5 Grounding 11 Digital system +3.3V
6 Digital system +5V
4.2.3 VERIFICATION OF THE VOLTAGE TO THE MAINBOARD
Procedure:
1. Turn OFF the CYANSmart analyzer.
2. Remove the cover of the instrument by unscrewing the 6 hex/allen screws on the bottom plate. Remove
gently because the connections to the touch screen remain attached!
3. Turn ON the CYANSmart analyzer.
4. Measure the voltage on the mainboard:
a. Test point 1 on picture above compared to the grounding of test point 5 or a screw. The measured
voltage should be 24V DC.
b. Or measure the voltage directly on the connector of the power input on the mainboard (see photo
above). Also here the measured voltage should be 24V DC.
If the voltage is not correct, please proceed to the verification of the power supply (see 2).

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③
⑤
⑥
②
①
④
⑦
②
④
⑥
4.2.4 REPLACEMENT OF THE MAINBOARD
If the input power is correct (24V), but any other test point is not correct, the main control board (CY009-
S13) needs to be replaced!
Procedure:
1. Turn OFF the CYANSmart analyzer.
2. Remove the cover of the instrument by unscrewing the 6 hex/allen screws on the bottom plate.
3. Disconnect all the connections of the mainboard (see 4.2.1).
4. Unscrew the 4 screws attaching the mainboard. Remove the main control board.
5. To install the new part, repeat the steps above in reverse order. Verify that all connections are correct
and firm.
6. After the installation perform a temperature calibration (see 4.6.2) and pump calibration (see 4.4.1)
NOTES:
In case of an electric shock, the settings saved on the mainboard might have been altered or deleted. Please
check and reset if necessary:
a) Temperature calibration (see 4.6.2)
b) Pump calibration (see 4.4.1)
c) Method programming in the menu “Methods” (see application sheet)
d) Settings: date and time, filter & cuvette …
4.3 OPTICAL SYSTEM
The optical system is the functional key compound of the instrument. It contains the light source, filters for
wavelength selection, flow cell with heating mechanism and a PC board that contains the photodiode
detector (pre-amplifier board). This pre-amplifier board also converts the light detection from an analog to
a digital signal for further processing on the mainboard.
1. Lamp
2. Filter wheel with filters
3. Motor for filter wheel
4. Home sensor of filter wheel
5. Flow cell
6. Pre-amplifier board with
photodetector
7. Peltier element
8. Temperature sensor
9. Fan of optical system
③
8
8
9

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4.3.1 LAMP
The 6V, 10W halogen lamp (1) is installed in a lamp holder (2), which is
installed on the optical system by a tightening screw (3).
The normal life span of the lamp is 2000 hours (= 1 year). The CYANSmart
has a power saving standby mode. After a specified time of inactivity,
the instrument will enter this standby mode which will diminish the
voltage to the lamp from 6V to 3V, thereby increasing the life time of the
lamp.
Upon replacement of the lamp, the height of the lamp in the holder
needs to be optimized (4.3.13).
If the analyzer is turned on, but the lamp does not emit light, please
proceed with the lamp replacement (see 4.3.13). Make sure to verify the
voltage on the mainboard before replacing the lamp (see 4.3.12).
4.3.2 FILTER WHEEL
The filter wheel (1) has 10 positions: one dark filter, 7 interferential filters (340 nm, 405 nm, 492 nm, 510 nm,
546 nm, 576 nm and 620 nm) and 2 free positions. The correct filter is aligned in the light path through a
cooperation between the filter wheel motor, the filter wheel home sensor (2) and the main control board.
All filters of the CYANSmart except the 340 nm, have a pre-filter installed before the filter. This is a non-
selective absorbent circle with a central hole with dimensions from 0.2 mm to 2.5 mm. The pre-filter will
decrease the light intensity of the light source before it passes the filter. By choosing a different diaphragm
for each filter, the difference in light intensity is equalized for all the filters. Because of its low light intensity,
the 340 nm filter has no pre-filter, and is thus regarded as the reference. The filters are kept in place by a
locking ring.
Keep in mind: the numbering on the filter wheel ( ) does not increase with the wavelength.
N° in software 1 2 3 4 5 6 7 8 9 0
Actual filter (nm) 340 405 492 510 546 578 620 Spare Spare Dark
N° on filter wheel 6 8 9 0 1 2 3 4 7 5
Pre-filter - 2.3 1.0 1.0 0.8 0.6 0.5
4.3.3 FLOW CELL
After the filter, the light passes through the flow cell that contains the sample to be measured. The light
path of the flow cell is 10 mm. The flow cell is heated with a Peltier element to 25°C, 30°C or 37°C and the
heating is regulated by a temperature sensor. To prevent overheating, the abundant heat is guided with a
heat sink towards the fan for the optical system. For more information on the heating mechanism,
calibration and replacement see 4.6 Temperature.
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Pre-filter Filter Locking ring
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4.3.4 PRE-AMPLIFIER BOARD
At the end of the light path the light is captured by the pre-
amplifier board (1), more precise the silicon photodiode (2) on
this board. The pre-amplifier board is thus responsible for
receiving the light particles and converting the light detection
from an analog to a digital signal for further processing on the
mainboard.
To better understand the processing of the light signal, it is crucial to understand the difference between 3
values that are obtained during an AD auto zero measurement.
Gain
The signal-amplification coefficient. This gain is automatically adjusted by the instrument
to obtain AD values between 50.000 and 60.000 up to a maximal gain of 29 is reached. The
instrument can measure a gain value in the range 1~30. The optimal value for
measurement is between 8 to16.
AD value
The amplified and converted (from analog to digital) signal. The AD value is expressed in
mV. It refers to the light intensity captured by the photodiode. The instrument is capable
of measuring AD values in a range of 0 – 65535. The optimal value for measurement is 80-
90% of the maximum value thus between 50000 to 60000.
Offset The dark voltage, used to compensate for (background) interference. The light beam is sent
through a black filter, so the pre-amplifier board does not receive any light signal from the
lamp or sample. The voltage measured is thus the (background) voltage of the circuit board
itself. The optimal value for measurement is between -150 to +150.
4.3.5 VERIFICATION OF THE OPTICAL SYSTEM
The AD zero menu permits to verify the performance of the whole optical system (lamp, filters, flowcell and
pre-amplifier board) and automatically adjust to compensate for aging of the lamp.
Furthermore the values of gain and offset are used in the calculation of the test results. Consequently, to
ensure precise and accurate measurements, it is strongly recommended to perform an AD zero:
 Every day/morning.
 If the AD auto zero is outside the range in the perform test menu.
 At instrument installation.
 After installation of a new lamp.
Before performing an AD Zero it is essential to check that:
a) The hydraulic system (flowcell + tubes) is clean and rinsed with distilled water.
b) The flowcell is filled with distilled water and without any air bubbles.
c) The instrument is ON for at least 30 min, because the lamp needs this time to stabilize.
d) The optical cover is closed.
Procedure:
1. Select the “AD Zero” menu. The result of the last performed AD zero is shown. It is recommended to
make a print-out of these results (by pressing the “Print” button) to be able to compare with the new
reading.
2. Position a tube with distilled water under the aspiration inlet tube and press the PUSH button to
aspirate the distilled water.
3. Pressing the “Read” button, the instrument will perform a new reading and display the results.
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www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [e-mail]
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Verify if the gain, AD and offset are within the acceptable range and the state is thus “OK” for all filters.
 Gain: between 8 and 16
 AD: between 50.000 and 60.000
 Offset: between -150 and +150
Since the AD Zero should be performed every day, the variation between the new measurement and the
previous (displayed when opening the menu) should be small. If you compare the results (always compare
the same filter!):
 The difference in gain should be ≤ 2
 The difference in offset should be ≤ ±50.
If the difference is bigger or the obtained values are outside the range, check first if:
1. The instrument is ON for at least 30 min, because the lamp needs this time to stabilize.
2. The hydraulic system (flowcell + tubes) is clean and rinsed abundantly with distilled water.
o Perform washing and rinsing (see 3.2.1). This washes both the flowcell and the tubes.
3. The windows of the flowcell are clean (no dirt/fingerprints on the outside).
o Clean with special wipes for glasses.
4. There is no crack or leakage in the flowcell:
o This can cause fluctuations in gain and AD value, the flowcell needs to be replaced (see4.3.6).
5. The flowcell is filled with distilled water. To ensure a correct measurement, the flowcell should be filled
with distilled water and free of air bubbles. This filling can be easily checked by opening the optical cover.
Check the tubes going to and from the flowcell:
o If the filling is not correct, verify the pump calibration (see 4.4.1) and the absence of leakages.
- The aspiration tube has to be completely
filled with liquid and free of air bubbles
from the fastening screw to the flowcell.
- The tube from the flowcell to the pump
should be filled with liquid and free of air
bubbles for at least 4 cm from the
flowcell.
This photo is made with a colored sample to
aid the visualization (instruction purposes).
The AD auto zero should always be
performed with distilled water!
If after these verifications, the values remain outside the ranges and
A. AD Zero out of range for one or few filters:
1. Verify the above points (1-5) even more thorough if (only) the 340 nm filter is out of range, because
this filter is the most sensitive to dirt or insufficient rinsing, proceed to 4.3.6 and only after this
continue to the next step.
2. Verification of the movement of the filter wheel (Proceed to 4.3.7 and 4.3.8 and 4.3.9 and 4.3.10).
Aspiration tube completely filled
with liquid, no air
bubbles
Tube from flowcell to pump, air bubbles
only visible after the 4 cm limit.

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [e-mail]
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3. Cleaning and/or replacement of the filter (Proceed to 4.3.11).
B. AD Zero out of range for all filters, check:
1. If the instrument is ON, but the lamp does not give light, it needs to be replaced. Make sure to test
the voltage on the mainboard before replacing it (Proceed to 4.3.12 & 4.3.13).
2. The flowcell is in the correct direction (windows to front and back) and completely inserted to the
bottom of the flowcell holder (see attention points 4.3.6).
3. The optical cover is closed during measurement.
4. Verification of the movement of the filter wheel (Proceed to 4.3.7, 4.3.8, 4.3.9 and 4.3.10)
5. The gain of all/most filters is 15, 16 or more.
 The lamp is aged and should be replaced (Proceed to 4.3.12).
4.3.6 CLEANING AND/OR REPLACEMENT OF THE FLOWCELL
A. Cleaning of the flowcell
In some cases, the normal hydraulic washing is not sufficient to clean away dirt attached to the inside of the
flowcell, and some ‘force’ is necessary to clean the flowcell.
Procedure:
1. Aspirate air to make sure all tubes are dry and will not leak upon disconnection.
2. Turn OFF the CYANSmart analyzer.
3. Carefully detach both tubes form the flowcell and take the flowcell out of the support.
4. Insert/attach a syringe filled with 5% Hypochlorite (bleach) in (small) inlet of the
flowcell.
5. Put force on the syringe to flush the flowcell. Particles that were adhered inside the
flowcell, will be flushed with the liquid through the (bigger) outlet tube.
6. Incubate the 5% Hypochlorite solution for 10 min (no longer) in the flow cell.
7. Rinse the flowcell abundantly with distilled water.
B. Replacement of the flowcell
When even the above cleaning procedure is not sufficient or when there is a crack or leakage in the flowcell,
the flowcell needs to be replaced.
Procedure:
1. Aspirate air to make sure all tubes are dry and will not leak upon disconnection.
2. Turn OFF the CYANSmart analyzer.
3. Carefully detach both tubes form the flowcell and take the flowcell out of the support.
4. To install the new part, repeat the steps above in reverse order.
ATTENTION POINTS!
1. Insert the flowcell in the correct direction! The light has to be able to pass through the windows of the
flowcell, thus the windows need to be facing to the front and to the back.
2. The inlet pipe (6) is thin, connected to the aspiration tube and should be facing forward. The outlet
tube (7) is thick, connected to the tube going to the peristaltic pump and should be facing to the back.
3. Insert the flowcell completely to the bottom of the support. Make sure nothing is blocking the
insertion!
4. Secure its position with the designated golden plate.

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4.3.7 VERIFICATION OF THE FILTER WHEEL HOME SENSOR
With this procedure the movement of the filter wheel and the correct positioning of the filters can be
checked. It must be followed when after the initial verification the filters remain out of range in the auto
zero reading or if an error is present in the self-test when starting up the instrument.
The requested filter is aligned in the light path through a cooperation between the filter wheel motor, the
filter wheel home sensor and the main control board. When the slit (1) in the filter wheel is between the 2
arms of the filter wheel sensor (2), this is detected and this is the ‘Home position’ of the filter movement.
From this position on, the mainboard will ‘instruct’ the number of necessary motor turns to position the
requested filter in the light path.
Procedure:
1. Turn ON the CYANSmart analyzer.
2. Select the “Filter test” menu.
3. Carefully remove the flowcell from the reading well (3) and position it in the socket for flowcell
protection (4).
4. Place a white paper of 1x5 cm in the reading well.
5. Select each filter separately and check the color displayed on the paper. Check that the color
corresponding to the requested filter is presented in a straight, well-aligned line (not scattered) :
340 nm  (ultra)Violet
405 nm  Dark blue
492 nm  Cyan
510 nm  Dark green
546 nm  Light green
578 nm  Yellow
620 nm  Red
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X
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window
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Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [e-mail]
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If the colors are correct and the light falling onto the paper is a straight, well-aligned line (not scattered),
but the AD values for most of the filters obtained are still out of range, continue to 4.3.12 & 4.3.13.
If the colors above are not correct or it takes long to find one or two filters (several times), please
continue with the cleaning and/or replacement of the home sensor (See 4.3.8).
If the filter wheel does not turn, continue with the verification and/or replacement of the filter wheel motor
(See 4.3.9 & 4.3.10).
If the colors are found correctly but the line of one or two filters is scattered, continue with the cleaning
and or replacement of those (pre)-filters (See 4.3.11) and then recheck the light scattering.
4.3.8 CLEANING AND/OR REPLACEMENT OF THE FILTER WHEEL HOME SENSOR
If the slit in the filter wheel or the home sensor itself are dirty, the home position cannot be detected.
Therefore, it is necessary to follow this procedure if the colors are not correct, if it takes long to find one
or two filters (several times), or if an error is present in the self-test when starting up the instrument.
A. Clean the slit in the filter wheel
Procedure:
1. Turn OFF the CYANSmart analyzer.
2. Remove the cover of the instrument by unscrewing the 6 hex/allen screws on the bottom plate.
3. Carefully remove the flowcell from the reading well and position it in the socket for flowcell protection.
4. Remove the tray of the optical system by unscrewing the 4 screws around the reading well and carefully
position the tray next to the optical system.
5. Remove the cover (1) of the filter wheel by unscrewing the hex/allen screw (2).
6. Turn the filter wheel manually (instrument is OFF!) until the slit is visible.
7. Clean the slit with (dry!) pressured air or by inserting strong paper. Make sure to remove all dirt.
B. Clean the home sensor of the filter wheel
Procedure:
Follow the steps 1-5 of the procedure for cleaning the slit of the filter wheel (A).
6. Disconnect the home sensor of the filter wheel from the mainboard (see 4.2.1).
7. Remove the 2 screws (3) attaching the home sensor and carefully take it out.
8. Clean the inside of the arms of the home sensor with (dry!) pressured air, with a soft brush or with special
wipes for cleaning glasses. Make sure to remove all (build-up) dust/dirt.
9. Reposition the sensor by repeating the above steps in reverse order.
ATTENTION: Make sure the filter wheel does not touch the home sensor while turning! The wheel should
be in the middle of the home sensor opening. Otherwise, readjust its position by adjusting the
tightening screws of the home sensor. Check visually by making a full turn of the wheel! When there is
a bend in the filter wheel, the optical system needs to be replaced (see 4.3.15).
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