Accuris SMARTREADER MULTIMODE User manual

Operation Manual
V1.0
This Product is for Research Use Only

Thank you for purchasing the SmartReader™MultiMode. This user manual details the
instrument’s features, specifications, as well as complete operating instructions;
please read it carefully before operation. Keep this user manual for later use.
Important:
Please keep the box and packaging material for this instrument. If service is
required, the box will be needed to ship the instrument to our Service
Department.
Initial Inspection
Please inspect the instrument as well as all included accessories when you first open
the packaging. If you find anything damaged or missing, please contact Benchmark
Scientific or your local distributor immediately.
BENCHMARK SCIENTIFIC / ACCURIS INSTRUMENTS
PO Box 709
Edison, NJ 08818
USA
Phone: [phone]
Website: www.benchmarkscientific.com / www.accuris-usa.com
Email: [email protected] / [e-mail]

Safety Warnings and Guidelines
1. Important information for safe use
Users should understand how to use this instrument before operating. Please
read this manual carefully prior to operation.
Any improper operation may cause injury. Please read this manual
carefully and operate safely according to the guidelines.
2. Operation and Maintenance
The operation and maintenance of the instrument should comply with the basic
guidelines and warnings below. Incorrect operation or maintenance will have
detrimental effects on the life, performance, and safety features of the instrument.
The instrument is a normal indoor instrument which conforms to classⅠof
the GB 4793.1 standard.
This instrument is designed for use in a laboratory environment. The
device must be operated by skilled laboratory personnel with appropriate
training.
To prevent injury or voiding the warranty, the operator should not attempt
to repair the instrument without explicit guidance from Accuris
Instruments. If service is required, please contact Accuris Instruments or
your local distributor for repair.
Before powering on, confirm that the voltage used meets the electrical
requirements of the instrument as stated on the rating plate. If the electric
cord is damaged, replace it with the same type of cord. Hold the socket
firmly before pulling the plug from an outlet. Do not pull the electric cord.
The instrument should be installed in an environment of standard room
temperature, low dust, low humidity, and away from direct sunlight,
electromagnetic interference, and heat sources. Do not block the vents on
the instrument.
Always power off the instrument when you are finished using it. Unplug
the power cord and cover the instrument with a cloth or plastic sheet to
prevent excessive dust from entering the housing.

Pull the connector plug from the electrical outlet immediately and
contact the vendor in the event of:
•Liquid entering the housing.
•Abnormal operation: such as any abnormal sound or smell.
•The instrument is dropped or there is any damage to the housing.
•Any malfunction.
3. Maintenance
The instrument should be kept in a dry environment away from electromagnetic
interference.
4. Transportation and storage requirements
Ambient temperature: 10℃~ 35℃
Relative humidity: ≤80%
Atmosphere pressure range: 500 ~ 1060hpa
Place in a well-ventilated room, away from corrosive gas.

Table of Contents
Chapter 1 Introduction ...................................................................................................1
1. Key Features......................................................................................................1
Chapter 2 Specifications................................................................................................2
1. Required Installation Environment.......................................................................2
2. Specifications .....................................................................................................2
Chapter 3 Instrument Overview.....................................................................................4
1. Structure.............................................................................................................4
Chapter 4 Instrument Setup...........................................................................................6
1. Preparation Before Use ......................................................................................6
Chapter 5 Programming & Operation............................................................................7
1. Instrument Startup...............................................................................................7
2. Quick Start Interface............................................................................................9
3. Step Settings Interface......................................................................................19
4. Analysis Interface..............................................................................................26
5. SmartDrop™Accessory Plate ...........................................................................37
6. Data & Report Exporting....................................................................................44
Chapter 6 Troubleshooting..........................................................................................45

─1 ─
Chapter 1 Introduction
The SmartReader™Multimode is a multi-detection instrument capable of performing
absorbance, fluorescence, and luminescence-based readings. Equipped with a
10-inch color touch screen, programming and operation is performed on-board. The
built-in software allows for multiple algorithmic analyses of standard curves,
qualitative & quantitative measurements, kinetics, spectroscopy, etc. An optional
security software (FDA 21 CFR Part 11) is available for this instrument that allows for
remote programming/operation via a PC.
1.1 Key Features
➢The instrument has a 10-inch color touch screen with built-in processing to
allow for on-board programming/operation. No PC connection is required.
➢The instrument has plate shaking capabilities (linear & orbital) and an
integrated incubator for accurate and uniform temperature control up to 45°C.
➢The instrument is equipped to perform the following detection methods:
•Absorbance (ABS)
•Fluorescence Intensity (FI)
•Fluorescence Resonance EnergyTransfer(FRET)
•Luminescence
➢Depending on the detection method, the instrument selects the measurement
wavelength by using either filters or a monochromator.
•ABS measurements utilize a monochromator.
•FI / FRET measurements utilize filters.
•Most luminescence-based measurements do not require wavelength
selection; however, a luminescence filter in included to eliminate
background noise during measurements.
➢The following accessories are available for this instrument:
•Automatic Injection Module (up to two) for automatic reagent addition.
•SmartDrop™Accessory Plate for microvolume analyses of nucleic
acids and proteins.
•21 CFR Part 11 compliant PC software

─2 ─
Chapter 2 Specifications
2.1 Required Installation Environment
Environmental temperature: 10 - 40C
Relative humidity: ≤80 %
Input voltage: AC 100 –240 V, 50 - 60 Hz, 2A
2.2 Specifications
Name
SmartReader™Multi-Mode (MR9620)
Measurement
Technologies
Absorbance, Fluorescence (FI, FRETF),
Luminescence (Glow, Flash)
Absorbance
Plate Formats
6, 12, 24, 48, 96 & 384-well microplates
Light Source
Xenon Flash Lamp
Wavelength Range
200 –1000 nm (1 nm steps)
Wavelength Accuracy
± 2 nm
Wavelength
Repeatability (SD)
0.2 nm
Bandwidth (FWHM)
< 2.5 nm
Measuring Range
0 - 4.0 OD
Resolution
0.0001 OD
Accuracy @ 450nm
± (1.0 % + 0.003) @ (0.0 - 2.0]
± 2.0 % @ (2.0 - 3.0]
Repeatability @ 450nm
CV <1.0% or SD <0.003 Fast mode (0.0 - 3.0]
CV <0.5% or SD <0.003 Precise mode (0.0 - 3.0]
Stability @ 450nm
< 0.005 Abs (0.0 - 2.0 Abs]
< 2% (2.0 - 3.0 Abs]
Linearity @ 450nm
R2 ≥0.999 [0.0 - 3.0 Abs]
Stray Light
0.1 % @ 220 nm
Reading Time
96 well: Fast mode: < 15 s; Precise Mode < 28 s
Fluorescence
Plate Formats
6, 12, 24, 48, 96 & 384-well microplates
Reading Mode
Top reading
Excitation Light Source
Xenon Flash Lamp
Detector
PMT
Wavelength Range
Excitation: 200 - 1000 nm
Emission: 270 - 850 nm
Filter EX / EM
Included: 470/525 nm, 523/564 nm, 624/692 nm
Detection Limit
≤1 pM
Linear Dynamic Range
6 logs

─3 ─
Luminescence
Plate Formats
6, 12, 24, 48, 96 & 384-well microplates
Detector
PMT
Detection Limit
100 amol / well
Linear Dynamic Range
6 logs
Crosstalk
≤0.005%
Shaking & Incubation
Shaking Mode
Linear (4.3 Hz, 7.0 Hz, & 13.5 Hz)
Orbital (180 RPM, 240 RPM, & 300 RPM)
Double Orbital ( 90 RPM, 120 RPM, & 150 RPM)
Shaking Frequency
Low, medium, high
Incubation
Temperature
RT+ 4°C –45°C
Temperature
Uniformity
± 0.5°C @ 37°C
Firmware
Display
10-inch LCD display
Operation
Touch screen; mouse
Data Capacity
10GB
Automatic Injection Module (AIM)
Plate Formats
96–well microplates
Capacity
Up to two
Dispensing Volume
5 –1000 μL, 1 μL increment
Liquid Injection Speed
125-500 μL / s
Accuracy
± 1 μL @ 5 - 50μL
± 2 % @ 51 - 1000μL
Waste Liquid
Collection
50 mL
General Specifications
Communication Ports
2 USB A type ports
1 USB B type port
1 Ethernet port
1 RS232 interface (AIM connection)
Size(W×D×H) cm
42.0 × 55.0 × 38.6 cm / 16.5 x 21.7 x 15.2 in.
Power Supply
AC 100 to 240 V, 50 to 60 Hz
Power
100-240 V, 2A
Weight
33 kg / 72.8 lbs.

─4 ─
Chapter 3 Instrument Overview
3.1 Structure
Front
Side Door
Figure 3.1.01 Front View
Figure 3.1.02 Side Door View
Filter (FL/LUM) Inlet
AIM Inlet
USB port
AIM Outlet
10-inch touch
screen
Operational
Front door
Keys
Side door
Plate holder
QR code scanning
module

─5 ─
Power socket
Back
Figure 3.1.03 Back View
Figure 3.1.04; Prior to inserting the filter cartridge, the QR code (found on the filter
cartridge) must be scanned by the QR code scanning module. The filter cartridge
should enter smoothly if inserted in the correct orientation.
Figure 3.1.04 Filter Cartridge Insertion
Cooling fan outlet
Internet port
RS232 port
USB port
PC port
Power switch

─6 ─
Chapter 4 Instrument Setup
4.1 Preparation Before Use
IMPORTANT: This instrument weighs 33 kg (72.8 lbs.) and requires two persons to
lift.
Remove the instrument from the shipping box. Place the instrument on a flat surface.
Open the side door; press the button on the head of the locking pin and pull up to
release the pin.
Note: The locking pin ensures that the microplate motor stays secure during
transport. Please pull out the locking pin before use.
Figure 4.1.01
Figure 4.1.02
Press
Release
Locking pin
Dustproof plug

─7 ─
Chapter 5 Programming & Operation
5.1 Instrument Startup
Power the instrument on. Upon start-up, the instrument will perform an automatic
calibration and self-check (Fig. 5.1.01).
Figure 5.1.01 Self Check Upon Startup
After calibration/self-check, the login screen will be displayed (Fig. 5.1.02).
Figure 5.1.02 Login Interface

─8 ─
Table 5 - 1 User Permissions
Admin
Administrator account: can view & edit user
permissions, generate system-wide logs, & add/delete
users.
Initial
password:
“0000”
User
Can only perform operative functions.
Note: Please store the set Admin password in a safe place. Contact Accuris Instruments if the
admin password has been lost.
Select “Account” to enter the Account-Admin interface (Fig. 5.1.03). To make
user-specific password changes, enter the “Password” tab (Fig. 5.1.04).
Figure 5.01.03 Account-Admin Interface
Figure 5.1.04 ChangeAccount Password
After entering the user-specific password, the Main Menu Interface is displayed (Fig.
5.1.05)

─9 ─
Figure 5.1.05 Main Menu Interface
Table 5 - 2 Main Menu Interface Operations
Name
Function
Quick Start
Select to create a new protocol.
All results
Select to view a list of all previously saved protocol results.
Standard
curve
Select to view the standard curve library
Share
Select to view a list of executed protocols, standard curves, etc.,
for transfer (USB, FTP, etc.)
Settings
Select to enter the settings interface
SmartDrop™
Accessory
Plate
Select to enter the SmartDrop Accessory Plate Interface. This
interface allows for microvolume analyses of Nucleic Acids &
Proteins using the SmartDrop Accessory Plate (not included).
Protocol
Select to view a list of protocols saved to this shortcut.
My favorites
Select to view a list of favorite protocols.
Recent
Select to view a list of the most recent protocols created/run

─10
5.2 Quick Start Interface
Select "Quick Start" on the main interface to enter the Quick Start interface. Users can
select protocol parameters according to experimental requirements (Fig. 5.2.01).
Figure 5.2.01 Quick Start Interface
There are three read modes: ABS, FL, LUM. The ABS mode has three reading types:
EndPoint, Kinetic, & Spectrum. FL & LUM only have two read types: EndPoint and
Kinetic. The Endpoint method only takes one reading at the end of a protocol,
whereas the Kinetic method performs readings during selected intervals, with a
minimum of two reads and a maximum of 99. The Spectrum method performs
readings within the designated wavelength range.
Table 5 - 3 Functions Available For ABS, FL, & LUM Readings
Endpoint
Kinetic
Spectrum
ABS
1.General
2.Shake
3.Advanced
4.Area Selection
1.General
2.Detection
3.Shake
4.Advanced
5.Area Selection
1. General
2. Shake
3. Advanced
4. Area Selection
FL
LUM
1.Injector
2.General
3.Shake
4.Advanced
5.Area Selection
1.Injector
2.General
3.Detection
4.Shake
5.Advanced
6.Area Selection

─11
The instrument can accept 6, 12, 24, 48, 96, & 384 well microplates. Table 5 - 4 lists
the types of compatible 96 & 384-well microplates for use with the instrument.
Table 5 –4 Compatible 96/384-well Plate Types
Well plate
Name
96 well plate
ANSI/SBS,clear,96-well
ANSI/SBS,black,96-well
ANSI/SBS,white,96-well
384 well plate
ANSI/SBS,clear,384-well
ANSI/SBS,black,384-well
ANSI/SBS,white,384-well
After selecting protocol parameters, select "OK" to enter the main protocol interface
(Fig. 5.2.02). It consists of title bar, main display area, menu bar and settings bar.
Figure 5.2.02 Main Protocol Interface
Menu Bar
Title Bar
Settings
Bar
Main Display Area

─12
5.2.01 Title Bar
The title bar can be used to return to the previous page, perform protocol operations,
name, and modify files (Fig 5.21.01).
Figure 5.21.01 Title Bar
Table 5 - 5 Legend: Title Bar Operations
Name
Functions
Return to the main menu. Click and a prompt box will pop up asking the
user to exit the current interface; click "OK" and another prompt box will
pop up "Do you want to save the current protocol?"; click "OK" to save
and exit; click "No" to exit without saving.
Name/Rename the current file. Click to display the input box to modify
the current name.
Protocol
Click to display operation options, including New, Save, Save as, Export
to U disk, scan QR code.
New
Click and the selection box (Fig. 5.21.02) will be displayed.
Save
Save the current file/results.
Save as
Save the protocol under a different name.
Export to U
disk
Export file to a USB drive (USB drive must be inserted into instrument
prior to performing this operation).
QR code
Generate a QR code for the data on the left of the standard curve.
Click the " ", and the input box for modifying the protocol name will pop up, (Fig.
5.21.02). The red text indicates that the new protocol name has already been used
once before.
Figure 5.21.02 Create a New Protocol

─13
5.2.02 Main Display Area
The default plate layout is set to a 96-well microplate format, consisting of 8 rows
(A-H) & 12 columns (1-12). Click A-H to select all the wells in the row; click 1-12 to
select all the wells in this column. Select “All” above row label “A” to select all the
wells of the plate.
In the Settings Bar, users can view Kinetic or Spectrum analyses. According to the
set reading type (Endpoint, Kinetic, Spectrum), the contents of the main display area
vary. Fig. 5.22.01 shows the results of an executed Endpoint protocol.
Figure 5.22.01 Endpoint Protocol Results
After the protocol is finished, the heat diagram is shown (Fig. 5.22.02).
Note: Only Endpoint protocols have heat diagrams.

─14
Figure 5.22.02 Heat Diagram Interface (Endpoint)
Fig. 5.22.03 shows the interface when a Kinetic protocol has finished.
Figure 5.22.03 Kinetic Protocol Results