cytiva pH/C-902 User manual

pH/C-902
Instruction
Important user information
All users must read the entire instructions to fully understand the
safe use of pH/C-902.
Safety notices
WARNING
WARNING indicates a hazardous situation
which, if not avoided, could result in death or
serious injury. It is important not to proceed until
all stated conditions are met and clearly
understood.
CAUTION
CAUTION indicates a hazardous situation which,
if not avoided, could result in minor or moderate
injury. It is important not to proceed until all
stated conditions are met and clearly
understood.
Note: A note is used to indicate information that is important for
trouble-free and optimal use of the product.
Declaration of conformity
This product meets the requirements of applicable CE-directives
when installed and used in an ÄKTAprocess™ system. A copy of the
corresponding Declaration of Conformity for the ÄKTAprocess
system is available on request.
The CE marking and the corresponding EU Declaration of Conformity
is valid for the instrument when it is:
•used according to the Operating Instructions or user manuals,
and
•used in the same state as it was delivered, except for alterations
described in the Operating Instructions or user manuals.
Introduction
The pH/C-902 Monitor is a high precision on-line monitor for
measurement of pH and conductivity in high performance liquid
chromatography applications.
The monitor is suitable for pH and conductivity measurements in
pharmaceutical applications where monitoring of running conditions
is critical.
The pH/C-902 Monitor is a combined monitor with two independent
measuring channels for both pH and conductivity.
The pH/C-902 features:
•Two separate pH measuring channels
•Two separate conductivity measuring channels
•Fast response
•High accuracy and reproducibility
•Flow cells with small dead volume
•Flow cells that can be connected close together, minimizing band
broadening and time delays between detectors.
•Control via UNICORN™ software only.
Safety
•The instrument is designed for indoor use only
•Do not use the instrument in a dusty environment or close to
spraying water
•Only use the instrument in accordance with local safety
regulations
WARNING
The instrument must not be opened by the user.
WARNING
Always disconnect the power supply before
attempting to replace any item on the
instrument during maintenance.
WARNING
The instrument power supply must be connected
to a grounded mains socket.
cytiva.com 14003958 AB

WARNING
When using hazardous chemicals, always use
protective glasses and other suitable personal
protection equipment.
Installation
The instrument is delivered pre-installed in ÄKTAprocess system
with all electrical connections prepared inside the electronics
cabinet. The conductivity cells and the pH electrode flow cell holder
are also mounted in proper positions.
WARNING
The electronics cabinet of the ÄKTAprocess
system must only be opened by personnel
authorized by Cytiva.
Operation
When the system is powered, the instrument is simultaneously
switched on. The monitor can be used immediately, but the full
specifications are not reached until after a warm-up period of
15 minutes.
The pH/C-902 Monitor has no user interface for the operator. It is
controlled entirely via UNICORN control software (version 5.0 or
later). The pH and conductivity values, as well as the temperature, are
displayed in the System Control module of the UNICORN control
software.
Note: The measured temperature is the temperature in the
conductivity flow cell, which can differ from the ambient
temperature.
Preparing the instrument
The following settings and calibrations may be necessary to perform
as described below:
•Set up conductivity temperature compensation
•Calibrate the conductivity cells
•Prepare and insert the pH electrode
•Calibrate the pH electrode
•Set up pH temperature compensation
Setting up the conductivity temperature compensation
The conductivity in a buffer is temperature dependent. Temperature
compensation should be used when relating conductivity to
concentration and/or comparing conductivity values. The
compensation consists of a compensation factor together with a
reference temperature. All conductivity values will then
automatically be converted to the set reference temperature.
In the UNICORN System Control module:
Step Action
1 Select System:Settings.
Result: The Instructions dialog box opens.
2 Select Monitors and click Cond_CIR101 or Cond_CIR102.
3 Enter a temperature compensation value between 0 and
9.99% in the CompFactor box.
4 Click OK.
The factor is expressed in percentage increase of conductivity per ℃
increase in temperature. If the temperature compensation factor is
unknown, a general approximate value of 2% can be set for many
common salt buffers.
Check the OFF-box in the Instructions dialog box for no
temperature compensation.
Calibrating the conductivity cell
Normally it is not necessary to adjust the cell constant as the flow cell
is pre-calibrated on delivery. The conductivity flow cell constant is
shown on the packaging and can be entered when the cell is
changed. Adjustment is only necessary when replacing the
conductivity cell with a flow cell whose constant is unknown.
However, it is also recommended that the flow cell is recalibrated
after cleaning.
The cell constant is adjusted in the UNICORN System Control
module.
Setting the constant for a new conductivity cell
The following instruction is applicable for a conductivity cell where
the constant value is known:
Step Action
1 Read the constant value from the packaging.
2 Selec System:Calibrate.
Result: The Calibration dialog box opens.
3 In the Monitor box, select CIT101_Cel or CIT102_Cel.
4 Type the constant value in the Reference Value 1 box.
5 Click the Read Value 1 button.
Result: The measured value is displayed in the Measured
value box.
6 Click the Close button to exit the dialog box.
Calibrating the conductivity cell
Note: The conductivity temperature compensation must not be
activated when the cell constant is adjusted. Set the
compensation to OFF in the Instructions dialog box.
Step Action
1 Prepare a calibration solution of 1.00 M NaCl, 58.44 g/l. Let the
solution rest until it is at room temperature. This is important
for exact measurements.
2 Fill the flow cell completely with the calibration solution by
pumping at least 15 ml through the cell with a syringe.
2 pH/C-902 Instruction 14003958 AB

Step Action
3 Stop the flow and wait 15 minutes until the temperature is
constant in the range of 20-30℃.
4 Read the displayed conductivity value and compare it to the
theoretical value at the temperature of the calibration solution
from the graph below.
No further actions are necessary if the displayed value and the
theoretical value correspond. If not, proceed with the following steps:
5 Select System:Calibrate.
Result: The Calibration dialog box opens.
6 In the Monitor box, select CIT101_Cal or CIT102_Cal.
7 Type the theoretical conductivity value according to the graph
below in the Reference Value 1 box.
8 Click the Read Value 1 button.
Result: The measured value is displayed in the Measured
value box. The new constant value is updated and displayed in
the Cell constant box.
9 Click the Close button to exit the dialog box.
Fig 1. Conductivity of 1.00 M NaCl at 20-30℃
Preparing and inserting the pH electrode
The tip of the pH electrode consists of a thin glass membrane.
protect it from breakage, contamination and drying out or the
electrode will be destroyed. Always store the electrode with the end
cover filled with a 1:1 mixture of pH 4 buffer and 1 MKO3. Do not store
in water only.
When it is not in use, e.g. during system CIP, service or storage of the
system, the pH electrode is replaced by a pH plug and the pH
electrode is placed in a holder that is attached to the frame adjacent
to the pH monitor
The instruction below describes how to insert the pH electrode:
Step Action
1 Unscrew the plastic fastening nut from the pH cell and remove
the pH plug.
2 Remove the pH electrode from its holder and take off the
protective cap. rinse the electrode with pure water.
Note:
The pH plug may be placed in the pH electrode holder while the
electrode is in use
3 Remove the fastening nut from the pH plug, insert the
electrode into the electrode adaptor and slide the nut over the
adaptor.
4 Carefully insert the electrode in the flow cell. Tighten the nut by
hand to secure the electrode.
5 Connect the monitor cable to the electrode.
6 Connect the grounding wire to the pH cell.
Fig 2. Inserting pH electrode into the flow cell
Note: if the electrode is not fully inserted, the system will leak and a
dead volume will occur in the holder.
CAUTION
Handle the pH electrode with care
Note: When running chromatography using organic solvents it is
recommended that the pH electrode is removed and the
dummy electrode is inserted in its place, as organic solvents
will cause pH electrode degeneration.
pH/C-902 Instruction 14003958 AB 3

Calibrating the pH electrode
A proper laboratory routine is to calibrate the instrument once a day,
when the electrode is replaced and if the ambient temperature is
changed. The pH monitor is calibrated using standard buffer
solutions in a two point calibration. The two buffer solutions can have
any pH value as long as the difference between them is at least 1 pH
unit.
The calibration can be performed with the pH electrode either fitted
in or removed from the flow cell.
Calibrating with the electrode outside the flow cell
When calibrating the electrode out of the flow cell and changing from
one buffer to another, rinse the electrode tip with distilled water and
dab it carefully with a soft tissue to absorb the remaining water. Do
NOT wipe the electrode as this may charge it and give unstable
readings.
Step Action
1 Remove the pH electrode from the flow cell and immerse the
electrode in the first standard buffer solution (normally pH 7.0).
2 Select System:Calibrate in the UNICORN System Control
module.
Result: The Calibration dialog box opens.
3 In the Monitor box, select pH_AIT121.
4 Enter the first reference pH value in the Reference value 1
box.
5 Click the Read Value 1 button.
Result: The measured value is displayed in the Measured
value box.
6 Rinse the electrode tip with distilled water and then immerse
the electrode in the second standard buffer solution (e.g. pH
4.0 or 9.0).
7 Enter the second reference pH value in the Reference value 2
box.
8 Click the Read Value 2 button.
Result: The measured value is displayed in the Measured
value box.
The Calibrated electrode slope box shows the slope of the
calibration curve where 100% corresponds to 59.16 mV per pH step at
25°C. The Asymmetry potential at pH 7 is shown as a mV value.
9 Before use, rinse the electrode using distilled water.
Calibrating with the electrode in the flow cell
Follow the same procedure as above. Ensure that the pH value has
stabilized before reading. Leave the pump running while calibrating.
Switch to the second standard buffer solution and repeat the
procedure.
Fig 3. Relationship between pH and the monitor output signal in mV
A new electrode typically has a slope of 95 – 102% and an asymmetry
potential within ±30 mV. As the electrode ages the slope decreases
and the asymmetry potential increases.
As a rule, an electrode should be replaced when it has an asymmetry
potential outside of ±60 mV and a slope lower than 80%, and no
improvement can be achieved by cleaning.
An electrode is still usable at lower slopes and higher asymmetry
potentials but the response will be slower and the accuracy
diminished.
Setting up pH temperature compensation
The relationship between pH and the output signal from the pH
electrode is temperature dependent. For more accurate
measurements during temperature changes, the pH measurement
can be temperature compensated. In normal applications, if the
temperature of the buffers and calibration buffers are identical,
temperature compensation does not need to be on.
When using temperature compensation it is important that the
temperature of the pH electrode is the same as that of the
conductivity flow cell since that is where the temperature is
measured.
The temperature compensation is activated in the UNICORN System
Control module:
Step Action
1 Select System:Settings.
Result: The Instructions dialog box opens.
2 Select Monitors.
3 Select pH_AIA121_TC.
4 Click the ON radio button.
5 Click OK to close the dialog box.
Storage
CAUTION
Never leave the pH electrode in the flow cell for
any period of time when the system is not used
since this may cause the glass membrane of the
electrode to dry out.
4 pH/C-902 Instruction 14003958 AB

Storage of the pH electrode
Step Action
1 Always dismount the pH electrode from the flow cell and fit the
end cover filled with a 1:1 mixture of pH 4 buffer and 1 M KNO3.
Do NOT store in water only.
2 Replace the pH electrode with the pH plug in the flow cell.
Regeneration of the pH electrode
If the electrode has dried out, immerse the lower end of the electrode
in buffer with a 1:1 mixture of pH 4 buffer and 1 M KNO3 overnight.
Storage of the conductivity flow cells
•Overnight: The conductivity cell can be left filled with a buffer.
•Weekend or long-term storage: Flush the conductivity cell
with water and fill the cell with 20% ethanol.
Restart after power failure
If the power supply to the instrument is interrupted, the instrument
automatically restarts itself when the power is resumed. All set
values are retained in the instrument.
Maintenance
WARNING
Disconnect the power supply before attempting
to replace any item on the instrument during
maintenance.
Only personnel authorized by Cytiva may service
the instrument.
CAUTION
Only spare parts approved or supplied by Cytiva
may be used for maintaining and servicing the
instrument.
Periodic maintenance
Interval Action
Every 6 months or more often if
required
Change pH electrode
When required Clean the conductivity cell
Clean the pH electrode
See procedures below
Cleaning the flow cell
Step Action
1 Remove the pH electrode and replace it with the pH plug in the
pH flow cell.
2 Pump a cleaning or sanitizing agent through the flow cells.
The standard recommendation is to pump 1 M NaOH for 30 minutes
and then wash out with buffer.
WARNING
NaOH is corrosive and therefore dangerous to
health. When using hazardous chemicals avoid
spillage and wear protective glasses and other
suitable personal protection equipment.
Cleaning the pH electrode
Note: The pH electrode has a limited life length and should be
replaced every six months or when the response time is slow.
Use one of the following procedures to clean the electrode to
improve the response:
Deposit Procedure
Salt Dissolve the deposit by immersing the
electrode first in 0.1 M HCl, then in
0.1 M NaOH, and again in 0.1 M HCl. Each
immersion should last for 5 minutes. Rinse
the electrode tip in distilled water between
each solution.
Oil or Grease Films Wash electrode tip in a liquid detergent and
water. If film is known to be soluble in a
particular organic solvent, wash with this
solvent. Rinse electrode tip in distilled water.
Protein Dissolve the deposit by immersing the
electrode in a 1% pepsin solution, in
0.1 M HCl, for 5 minutes, followed by a
thorough rinsing with distilled water.
If these procedure fail to rejuvenate the electrode, the problem is
most likely a clogged liquid junction.
Step Action
1 Heat a 1 M KNO3 solution to 60 – 80°C.
2 Place the electrode tip in the heated KNO3 solution.
3 Allow the electrode to cool while immersed in the KNO3
solution before retesting.
If these steps fail to improve the response of the electrode, it should
be replaced.
Changing the pH electrode
See Section Preparing and inserting the pH electrode, .
Cleaning the conductivity flow cell
If the conductivity measurements are not comparable to previous
results, the electrodes in the flow cell may be contaminated and
require cleaning. To clean the flow cell:
Step Action
1 Pump 15 ml of 1 M NaOH at 1 ml/min through the flow cell
either by using the pump or a syringe.
2 Leave it for 15 minutes.
3 Rinse thoroughly with 50 ml de-ionized water.
Note: If the flow cell is totally blocked, the blockage can be broken
using a thin needle or a piece of string with a diameter of less
than 0.8 mm.
pH/C-902 Instruction 14003958 AB 5

Changing conductivity flow cells
The flow cells can be changed when required. Make sure that the
system is disconnected from the mains power and de-pressurized
before disconnecting/ connecting the flow cells.
If the cell is replaced with a new flow cell, the monitor must be
calibrated with the new cell constant value which is written on the
flow cell package. See Section Calibrating the conductivity cell, .
Troubleshooting
General
When contacting Cytiva for support, access the UNICORN
Maintenance Manager in the System Control module to display the
instrument information, e.g. version number, for reference.
Step Action
1 Select System:Maintenance.
2 Select the Info tab.
3 Click the pH/Cond icon.
4 Click General.
WARNING
The electronics cabinet may only be opened by
Cytiva authorized personnel.
Faults and actions
If the suggested actions do not correct the fault, call Cytiva.
pH measurement
Fault Action
No response to pH
changes
1. Check that the electrode cable is
connected properly to the electrode.
2. The electrode glass membrane may be
cracked.
Replace the electrode.
Small response to pH
changes
1. Clean the pH electrode.
2. If the problem remains, replace the pH
electrode.
Slow pH response or
Calibration impossible
1. Check the pH electrode tip. If it is
contaminated, clean the electrode.
2. If the membrane has dried out, the
electrode may be restored by soaking it
in buffer overnight.
3. Clean the liquid junction.
Incorrect / unstable pH
reading
1. Check that the electrode cable is
connected properly to the electrode.
2. Check that the pump(s) and valves
operates correctly.
3. Check that the electrode is correctly
inserted in the flow cell and, if
necessary, hand-tighten the nut.
Fault Action
4. If air in the flow cell is suspected, tap
the flow cell or tilt it to remove the air.
Alternatively flush the flow cell with
buffer at 8 ml/min for 1/2 min.
5. Check that the pH electrode is not
broken.
6. Check that the pH electrode is
calibrated.
7. Check the slope. If it is outside the
range 80 – 105% or the asymmetry
potential deviates more than 60 mV
from 0mV, clean the pH electrode.
Recalibrate and if the problem
persists, replace the pH electrode.
8. Clean the pH electrode if required.
9. Compare the response of the pH
electrode with that of another pH
electrode. If the response differ
greatly, the electrode may require
cleaning or replacement.
10. Check that the pH electrode has been
calibrated at the correct temperature.
11. In organic solvents such as ethanol,
methanol and acetonitrile, stable pH
measurements are not possible since
dehydration of the membrane will
occur. It is recommended that the pH
electrode is not used in applications
using organic solvents. Replace the
electrode with the pH plug.
12. Clean the liquid junction.
pH values vary with
varying back-pressure
Replace the pH electrode.
Conductivity measurement
Fault Action
Incorrect or unstable
reading
1. Check that the conductivity flow cell
cable is connected properly.
2. Check that the pump(s) and valves
operate correctly.
3. If temperature compensation is being
used, check that the correct
temperature compensation factor is in
use.
4. Check that the column is equilibrated. If
necessary, clean the column.
Baseline drift or noisy
signal
1. There may be air in the flow cell. Use a
flow restrictor after the flow cell.
2. Check for leaking tubing connections.
3. Check that the column is equilibrated. If
necessary, clean the column.
4. Check the operation of the pump(s).
5. Clean the flow cell.
6 pH/C-902 Instruction 14003958 AB

Fault Action
Conductivity
measurement with the
same buffer appears to
decrease over time
1. Clean the flow cell.
2. The ambient temperature may have
decreased. Use a temperature
compensation factor. (See Section
Setting up the conductivity
temperature compensation, )
Absolute conductivity
value wrong
1. Recalibrate the conductivity cell.
2. The calibration solution, 1.00 M NaCl
was not correctly prepared. Prepare a
new calibration solution and
recalibrate the conductivity cell.
Ghost peaks appear in
the gradient profile
1. A charged sample has been detected
(e.g. protein)
2. Air bubbles are passing through the
flow cell. Check for loose tubing
connections. If necessary use a flow
restrictor after the conductivity flow
cell.
Error messages
If the suggested actions do not correct the fault, call Cytiva.
pH monitor
Error Help text message
pH not offset calibrated Must be factory calibrated, contact
service engineer.
Error in temp sensor If conductivity cell (where temperature
sensor is located) is not connected,
connect cell and restart system. If error
message reappears or conductivity cell is
already connected, contact service
engineer.
Warning: Bad slope Slope is < 70% or >100%. Clean the pH
electrode. If message reappears, replace
the pH electrode.
pH calibration failed Very bad slope, <10% or >199%. Check pH
cell connection. Recalibrate. If error
message reappears, replace the pH
electrode.
pH too close betw buff
1&2
The difference between pH of the buffers
used during calibration must be at least
1 pH unit.
pH-value is out of range pH must be in the range 0-14.
Bad or not conn pH
electr.
Defect pH electrode or pH electrode not
connected.
Error factory calibration
pH
Factory calibration of negative offset
must be repeated, contact service
engineer
Conductivity monitor
Error Help text message
Cell const out of range 1. Wrong solution used during
calibration. Recalibrate.
2. Air in conductivity cell during
calibration. Flush the flow cell with
calibration solution and recalibrate.
Error Help text message
3. Dirty conductivity cell. Clean the cell.
Recalibrate.
4. Bad cell. Replace.
Error cond hardware Restart system. If error message
reappears, call service engineer.
Incorrect filter size Enter correct filter size.
Temperature monitor
Error Help text message
Error in temp sensor Temperature sensor in conductivity cell X
is broken or conductivity cell is not
connected. If conductivity cell is not
connected, connect cell and restart
system. If error message reappears or
conductivity cell is already connected,
contact service engineer.
Error temp comp Set correct temperature compensation
(0-9.95%) or if conductivity cell is not
connected, connect cell and restart
system. If error message reappears or
conductivity cell is already connected,
contact service engineer.
Error in temp sensor If conductivity cell (where temperature
sensor is located) is not connected,
connect cell and restart system. If error
message reappears or conductivity cell is
already connected, contact service
engineer.
Ref temp out of range Enter correct reference temperature
(0-99.9°C).
Reference information
pH electrode
The pH electrode is of the sealed combination double junction type. It
contains a sealed Ag/AgCl reference which cannot be refilled, an
internal electrolyte bridge of 4M KCl saturated with Ag/AgCl, an
outer electrolyte bridge of 1 M KNO3, an annular ceramic reference
junction and a low profile pH membrane. The pH electrode is
delivered with a transparent cover.
The flow cell should not be used with another pH electrode.
pH/C-902 Instruction 14003958 AB 7

Fig 4. pH electrode cross-section
Conductivity cell
The flow cell has two cylindrical titanium electrodes positioned in the
flow path of the cell. An alternating voltage is applied between the
electrodes and the resulting current is measured and used to
calculate the conductivity of the eluent. The monitor controls the AC
frequency and increases it with increasing conductivity between
50 Hz and 50 kHz giving maximum linearity and true conductivity
values.
The conductivity is automatically calculated by multiplying the
measured conductance by the flow cell’s cell constant. The cell
constant is pre-calibrated on delivery but can be measured with a
separate calibration procedure. This procedure is described in
Section Calibrating the conductivity cell, .
One of the electrodes has a small temperature sensor for measuring
the temperature of the eluent in the flow cell. Temperature variations
influence the conductivity and in some applications, when highly
precise conductivity values are required, it is possible to program a
temperature compensation factor that recalculates the conductivity
to a set reference temperature.
Fig 5. Conductivity cell – principle
Technical specifications
Operating data
General
Operating temperature
for monitor
4 – 40°C
Operating surrounding
temperature for cells
2 – 30°C
Surrounding humidity 20 – 95% rH
pH measurement
pH range 0 to 14 (spec. valid between 2 and 12)
Accuracy
temperature
compensated1
±0.1 pH within +4 to +40°C
not temperature
compensated1
±0.2 pH within +15 to +25°C
±0.5 pH within +4 to +40°C
Response time Max 150 s (0-95% of step)
Long term drift Max 0.1 pH units/8 h
(constant conditions)
Flow rate sensitivity Max 0.2 pH units within 50l/h - 200 l/h
20°C
1Calibrated for 20°C
Conductivity measurement
Conductivity range 1 μS/cm - 999.9 mS/cm
Deviation from true
conductivity value
Max ± 4 % or ±10 μS/cm, whichever is
greater, in the range 1 μS/cm –
300 mS/cm
Max ± 2 % or ±10 μS/cm, whichever is
greater, in the range 0,1 μS/cm –
100 mS/cm (at room temperature)
Reproducibility
short term Max ±1 % or ±5 μS/cm
long term Max ±3 % or ±15 μS/cm
Noise ±0.5% of full scale calibrated range
Response time Max. 120 s (0 – 95% of step)
Temperature sensor
Accuracy ±2.0°C
Drift ±1%/10 h
Flow rate sensitivity ±1% within 50 l/h – 200 l/h 20°C
Flow cell data
pH cell
Cell sizes:
6 mm For use in:
6mm Polypropylene systems
3/8” Stainless steel systems
10 mm For use in:
10mm Polypropylene systems
1/2” Stainless steel systems
22 mm For use in:
1” systems (PP and SS)
8 pH/C-902 Instruction 14003958 AB

pH cell
Flow range 4 – 180 l/h (6 mm cell)
13 – 600 l/h (10 mm cell)
45 – 1800 l/h (22 mm cell)
Max pressure 10 bar (6 and 10 mm cell)
6 bar (22 mm cell)
3 bar up to +60°C (all cells)
1 bar up to +80°C (all cells)
Back pressure Max 0.1 bar at 600 l/h (6 and 10 mm cell)
Max 0.1 bar at 1800 l/h (22 mm cell)
Wetted materials PEEK and Polypropylene (polypropylene
systems)
PEEK and EN 1.4435 (stainless steel
systems)
Sealing: EPDM, FEP encapsulated silicone
Chemical resistance The wetted parts are resistant to organic
solvents and salt buffers commonly used
in chromatography of biomolecules,
except 100% Ethyl acetate, 100% Hexane
and 100% Tetrahydrofuran (THF)
Conductivity cell
Cell sizes:
8 mm For use in:
6 and 10 mm Polypropylene systems
3/8” and 1/2” Stainless steel systems
12 mm For use in:
1” Polypropylene systems
1” Stainless steel systems
Flow range 4 – 600 l/h (8 mm cell)
45 – 1800 l/h (12 mm cell)
Max pressure 10 bar (8 mm cell)
6 bar (12 mm cell)
3 bar up to +60°C (all cells)
1 bar up to +80°C (all cells)
Back pressure Max 0.1 bar at 600 l/h (8 mm cell)
Conductivity cell
Max 0.2 bar at 1800 l/h (12 mm cell)
Wetted materials PEEK, Polypropylene and Titanium
Sealing: EPDM, FEP encapsulated silicone
Chemical resistance The wetted parts are resistant to organic
solvents and salt buffers commonly used
in chromatography of biomolecules,
except 100% Ethyl acetate, 100% Hexane
and 100% Tetrahydrofuran (THF)
Accessories and spare parts
Item Quant Code no.
Transmitter pH/C-902 1 28-4019-33
pH electrode CPS11-1BA2ESA 1 44-5514-99
pH Cable CPK9 TOP68 L=5 1 44-5514-85
O-ring kit pH PFR91 1 44-3004-70
pH flowcell PEEK/PFR91 Process
connection: 3/4” Tri-Clamp (TC25), ID
9.4mm
1 28-4022-28
Conductivity sensor 8mm EPDM 1 28-4021-98
Conductivity sensor 8mm FEP 1 28-4021-99
O-ring kit CM-C EPDM
2pcs O-ring 25.07x2.62 EPDM
6pcs O-ring 10.78x2.62 EPDM
1pc O-ring 58x3.0 EPDM
1pc Assembly rod
1 28-4022-00
O-ring kit CM-C FEP
2pcs O-ring 25.07x2.62 EPDM
6pcs O-ring 10.77x2.62 FEP
1pc O-ring 58x3.0 EPDM
1pc Assembly rod
1 28-4022-01
cytiva.com/bioprocess
Cytiva and the Drop logo are trademarks of Global Life Sciences IP Holdco LLC or an
affiliate.
ÄKTAprocess and UNICORN are trademarks of Global Life Sciences Solutions USA
LLC or an affiliate doing business as Cytiva.
All other third-party trademarks are the property of their respective owners.
© 2020–2021 Cytiva
All goods and services are sold subject to the terms and conditions of sale of the
supplying company operating within the Cytiva business. A copy of those terms and
conditions is available on request. Contact your local Cytiva representative for the
most current information.
For local office contact information, visit cytiva.com/contact
14003958 AB V:5 05/2021