CytoTherm CT-D4 User manual

110 Sewell Ave. Trenton NJ 08610 USA
Tel 800 747
Email serve@cytotherm.com Web www.cytotherm.com
CytoTherm
Dry Plasma
Thawing
with
Massaging Action
Model
CT-D4
Ver 409 and
higher
Owner’s Manual
W A R R A N T Y
Each CytoTherm
product is manufactured under rigid quality control standards. This unit is
fully warranted for a period of
two
9699 for assistance. If necessary send unit to:
CytoTherm 110 Sewell Av
e. Trenton, NJ USA Tel 609 396
Please record the following: Serial No. ___________ Date of Purchase _________
TABLE OF CONTENTS
1 Specifications
2 Description
2 Setting-up
3 Operation
3 Leaks
5 Bladder Replacement
5 Modes
SPECIFICATIONS:
Thawing temperature adjustable 25
over thawing temperature. 45
o
C safety thermostat. Accuracy 0.1
900
Watt, Available in 120 Volt or 230 Volts, 50 or 60 Hz. (must be grounded).
CytoTherm
110 Sewell Ave. Trenton NJ 08610 USA
Tel 800 747
-9699 or 609 396-1456 Fax 609 396-
9395
Email serve@cytotherm.com Web www.cytotherm.com
CytoTherm
-D4
Thawing
Massaging Action
W A R R A N T Y
product is manufactured under rigid quality control standards. This unit is
two
years
from the date of purchase. Call 609 396
9699 for assistance. If necessary send unit to:
e. Trenton, NJ USA Tel 609 396
-
1456 Fax 609 396
Please record the following: Serial No. ___________ Date of Purchase _________
TABLE OF CONTENTS
Draw\Instruct CT Products\
CTD4ins
7 Troubleshooting
8 Maintenance
9 Parts List
9 Technician Functions
10 Safety Features
11 Wiring Diagram
Thawing temperature adjustable 25
o
C to 40
o
C. Preset to 37.0
o
C safety thermostat. Accuracy 0.1
o
C. Easy to do, digital temperature calibration. Power
Watt, Available in 120 Volt or 230 Volts, 50 or 60 Hz. (must be grounded).
9395
Email serve@cytotherm.com Web www.cytotherm.com
product is manufactured under rigid quality control standards. This unit is
from the date of purchase. Call 609 396
-1456 or 800 747-
1456 Fax 609 396
-9395
Please record the following: Serial No. ___________ Date of Purchase _________
CTD4ins
P.doc 27 Jun 16
o
C. Overtemp alarm 1.0
o
C
C. Easy to do, digital temperature calibration. Power

2
DESCRIPTION
The CytoTherm-D4 (Model CT-D4) is a laboratory instrument for thawing
plasma while keeping the plasma bag dry. Tempered water is recirculated
through a bladder. The plasma bag to be thawed lays on one part of the
bladder, while rest of the bladder is folded over and covers the plasma
bag(s). The bladder is divided into segments which are pressurized at
different times to gently massage the plasma bag(s), thereby speeding
thawing. The plasma bag(s) remains dry.
There are 2 isolated sections. If a leak is detected, the thawing stops and an alarm sounds. The plasma bags in the other
section remains sterile.
A digital timer displays the time to completion of the thawing. The plasma bag is visible through the clear lid during
thawing so you can see when it has been fully thawed.
Each of the 2 sections can thaw two 450ml. bags or one 1,000ml. Jumbo bag for a total of 4 x 450ml. or 2 Jumbo bags.
All the air that comes in contact with the internal water is filtered with a 0.1 micron AIR FILTER.
SETTING-UP
The unit comes packed in 1 box. Make sure you have received the following:
….. Main Bath ….. 6 Y shaped blue silicone accelerators
….. Rack ….. Inlet and Outlet Port Plugs (4 of each)
….. Lid ….. Instruction Book
….. 4 Bladders, 2 of which are mounted inside RACK ….. Power Cord
….. Drain tubing ….. Algaecide (contiguous USA only)
….. 4 x Air Filters
For video instructions, visit www.cytotherm.com.
Place the Bath on a counter. Fill with tap water to the Water label inside the BATH. If you use deionized water, add a
pinch of salt to make the water conductive. Do NOT use saline solution. Add 3 ml. of Algicide Part #ALG240 (1.0 ml. per 4
liter of water available in contiguous USA only) to the water. Any other bath clearing agent can also be used.
Place the RACK and LID on the BATH. Lift the front of the rack and attach the tubing from the Bath right side to the
RACK left side fitting. Attach the BATH right side tubing to the RACK left side fitting. Push the front of the RACK down
and towards the center of the bath to engage the leak detector connectors (SS springs). Push the back of the RACK down
to squeeze the air seal.
The RACK has 2 bladders mounted inside. They are easy to remove and replace by just twisting the bladder FITTINGS
as you push down or pull up on the bladder FITTINGS.
Plug the unit in and turn it on. The bath will warm up and maintain the programmed thawing temperature, preset to
37.0
o
C.
Plasma
Plasma
AA
A
A
AA
A
A
B
BB
B

OPERATION
Wait for the water to heat up to 37.0
o
C. Open the LID by:
1. Pushing the LOCKBAR to the left.
2. Lifting the front of the LID until it is vertical.
3. Lowering the LID into the slot .
Lift the top of the BLADDER and place the plasma bags on the bottom part of the
BLADDER. Pl
ace the "Y" accelerators on the middle of each plasma bag. Cover
the plasma bags with the top portion of the bladder. Tuck in the BLADDER so that
it stays within the walls of the section of the RACK.
Close the LID by lifting straight up until it stops
(forward). Lock it into place by pushing the LOCKBAR to the right.
Push START to begin thawing for the programmed time. The pumps will pump
tempered water through the BLADDER and massage the plasma bags. The
thawing time will be
displayed. Use PLUS or MINUS to increase or decrease the
thawing time. When the time has expired, the buzzer will call the operator back.
STOP ends the thawing and drains the bladders.
If more thawing time is needed, hold PLUS while pushing
START and th
e unit will thaw for another 3 minutes.
Thawing time is mainly a factor of thickness of the plasma in
the bag. Flat-
frozen plasma thaws quicker than folded
plasma, which is thicker.
LEAKS
In case of a leak, the alarm will sound and the thawing will stop.
Open the LID to see which bag leaked. The leak will be confined
to only one of the sections. The other section is not contaminated.
Determine what leaked:
1. Plasma bag leaked. Pick
up the plasma bag and
observe for leaks. If there is a leak, discard the bag(s).
Check if the Bladder also leaked. See # 3 below. If the BLADDER did not leak, use a paper towel to soak up the
visible leaked solution. Remove the BLADDER by first lifting the
much of its internal water as possible. Remove the INLET and OUTLET FITTINGS by holding the
FITTINGS and twisting them as you pull up.
If you need to rush thawing the plasma bags in the non
later. Use the plastic plugs to close off the stainless steel ports, turn the unit on and start thawing. You will have
to watch the plasma bags to see when they are thawed, because the count down timer starts at the full thawin
time.
Rinse the BLADDER under flowing water and wipe with a bleach towelette or a 10% bleach solution.
Wipe out the inside of the RACK and then wipe it with a bleach towelette or a 10% bleach solution. Remount
the cleaned BLADDER in the RACK.
2. BLADDER leaked
. If the plasma bag did not leak, the leak must be in the bladder. Wipe off and place the
plasma bag(s) aside. Remove and discard the BLADDER by first lifting the top of the BLADDER up to drain
out as much of its internal water as possible.
FITTINGS and twisting them as you pull up. Wipe up all the leaked solution inside the RACK. Wipe the inside
of the RACK with a bleach towelette or a 10% bleach solution. Remount a new BLADDER. You are re
start thawing again.
3
C. Open the LID by:
2. Lifting the front of the LID until it is vertical.
Lift the top of the BLADDER and place the plasma bags on the bottom part of the
ace the "Y" accelerators on the middle of each plasma bag. Cover
the plasma bags with the top portion of the bladder. Tuck in the BLADDER so that
it stays within the walls of the section of the RACK.
Close the LID by lifting straight up until it stops
and then rotating it down
(forward). Lock it into place by pushing the LOCKBAR to the right.
Push START to begin thawing for the programmed time. The pumps will pump
tempered water through the BLADDER and massage the plasma bags. The
displayed. Use PLUS or MINUS to increase or decrease the
thawing time. When the time has expired, the buzzer will call the operator back.
STOP ends the thawing and drains the bladders.
If more thawing time is needed, hold PLUS while pushing
e unit will thaw for another 3 minutes.
Thawing time is mainly a factor of thickness of the plasma in
frozen plasma thaws quicker than folded
-frozen
In case of a leak, the alarm will sound and the thawing will stop.
Open the LID to see which bag leaked. The leak will be confined
to only one of the sections. The other section is not contaminated.
up the plasma bag and
observe for leaks. If there is a leak, discard the bag(s).
Check if the Bladder also leaked. See # 3 below. If the BLADDER did not leak, use a paper towel to soak up the
visible leaked solution. Remove the BLADDER by first lifting the
top of the BLADDER up to drain out as
much of its internal water as possible. Remove the INLET and OUTLET FITTINGS by holding the
FITTINGS and twisting them as you pull up.
If you need to rush thawing the plasma bags in the non
-contaminated section,
you can do the full clean up
later. Use the plastic plugs to close off the stainless steel ports, turn the unit on and start thawing. You will have
to watch the plasma bags to see when they are thawed, because the count down timer starts at the full thawin
Rinse the BLADDER under flowing water and wipe with a bleach towelette or a 10% bleach solution.
Wipe out the inside of the RACK and then wipe it with a bleach towelette or a 10% bleach solution. Remount
the cleaned BLADDER in the RACK.
. If the plasma bag did not leak, the leak must be in the bladder. Wipe off and place the
plasma bag(s) aside. Remove and discard the BLADDER by first lifting the top of the BLADDER up to drain
out as much of its internal water as possible.
Remove the INLET and OUTLET FITTINGS by holding the
FITTINGS and twisting them as you pull up. Wipe up all the leaked solution inside the RACK. Wipe the inside
of the RACK with a bleach towelette or a 10% bleach solution. Remount a new BLADDER. You are re
Check if the Bladder also leaked. See # 3 below. If the BLADDER did not leak, use a paper towel to soak up the
top of the BLADDER up to drain out as
much of its internal water as possible. Remove the INLET and OUTLET FITTINGS by holding the
you can do the full clean up
later. Use the plastic plugs to close off the stainless steel ports, turn the unit on and start thawing. You will have
to watch the plasma bags to see when they are thawed, because the count down timer starts at the full thawin
g
Rinse the BLADDER under flowing water and wipe with a bleach towelette or a 10% bleach solution.
Wipe out the inside of the RACK and then wipe it with a bleach towelette or a 10% bleach solution. Remount
. If the plasma bag did not leak, the leak must be in the bladder. Wipe off and place the
plasma bag(s) aside. Remove and discard the BLADDER by first lifting the top of the BLADDER up to drain
Remove the INLET and OUTLET FITTINGS by holding the
FITTINGS and twisting them as you pull up. Wipe up all the leaked solution inside the RACK. Wipe the inside
of the RACK with a bleach towelette or a 10% bleach solution. Remount a new BLADDER. You are re
ady to

3.
Plasma bag and BLADDER both leaked
leaked, discard the plasma bag(s) and squeeze down lightly on the BLADDER. If the bladder is also leaking,
the volume
of the leaked solution in the front of the RACK will increase. Remove the BLADDER (see #2
above) and discard it. Wipe the section of the RACK with bleach towelettes or a 10% bleach solution. DO NOT
USE CONCENTRATED BLEACH.
If you need to rush thawing the plasma bags in the sterile section, you can do the full clean up later. Use plugs
to close off the stainless steel ports, turn the unit on and start thawing. You will have to watch the plasma bags
to see when they are thawed
, because the count down timer will restart from the full thawing time.
Do a full clean up. Lift the front of the RACK, disconnect the RACK TUBING and place the RACK aside.
Drain all the water in the bath.
CT
-
Drain Tube Assembly
Part C-VLVM
Insert drain tube
clock wise to lock.
After water starts to flow, gently pinch the drain tube and release to
remove any air to improve flow.
4
Plasma bag and BLADDER both leaked
. It is a very unlikely case. After you determine that the plasma bag
leaked, discard the plasma bag(s) and squeeze down lightly on the BLADDER. If the bladder is also leaking,
of the leaked solution in the front of the RACK will increase. Remove the BLADDER (see #2
above) and discard it. Wipe the section of the RACK with bleach towelettes or a 10% bleach solution. DO NOT
USE CONCENTRATED BLEACH.
If you need to rush thawing the plasma bags in the sterile section, you can do the full clean up later. Use plugs
to close off the stainless steel ports, turn the unit on and start thawing. You will have to watch the plasma bags
, because the count down timer will restart from the full thawing time.
Do a full clean up. Lift the front of the RACK, disconnect the RACK TUBING and place the RACK aside.
-
D4 Quick Connect Drain
Insert drain tube
into drain housing part C-
VLMF.
clock wise to lock.
After water starts to flow, gently pinch the drain tube and release to
. It is a very unlikely case. After you determine that the plasma bag
leaked, discard the plasma bag(s) and squeeze down lightly on the BLADDER. If the bladder is also leaking,
of the leaked solution in the front of the RACK will increase. Remove the BLADDER (see #2
above) and discard it. Wipe the section of the RACK with bleach towelettes or a 10% bleach solution. DO NOT
If you need to rush thawing the plasma bags in the sterile section, you can do the full clean up later. Use plugs
to close off the stainless steel ports, turn the unit on and start thawing. You will have to watch the plasma bags
, because the count down timer will restart from the full thawing time.
Do a full clean up. Lift the front of the RACK, disconnect the RACK TUBING and place the RACK aside.
VLMF.
Push in and turn

5
Most of the water will drain out. Tilt the bath to drain more of the water. Wipe the inside of the
BATH and the RACK with a bleach towelette or a 10% bleach solution. Mount a new BLADDER to
replace the leaked BLADDER in the RACK. Reassemble (See SETTING-UP). Plug in and turn on
the unit and push START and let it run for a few minutes so that the water passes through and
rinses the pumps and the BLADDERS on the inside. Turn the unit off, drain out all the water a
second time. Reassemble (see SETTING-UP). The unit is ready to start thawing.
BLADDER REPLACEMENT
Hold the BLADDER so that the holes in the FITTINGS (water connectors) face down. Push the top of the
BLADDER underneath the black rod that passes across the RACK near the back of the RACK. Gently pull the
BLADDER up until the FITTING is lined up with the Stainless Steel PORT (Exit Tube) near the black rod.
Push down on the FITTING as you twist back and forth until the FITTING bottoms against the bottom of the
RACK.
Mount the other three FITTINGS the same way.
BLADDER STORAGE
When storing, roll the bladders up loosely, start with a 2.5 cm. (1”) diameter. Do not fold. Folding the bladder
will crimp the internal venturi tubing. If the bladder is dry it can be stored in a zip lock bag. If the bladder is
wet then allow air flow around the rolled bladder.
MODES
STANDBY MODE. Just turn the unit ON. The unit heats up and maintains 37.0
O
C in the BATH.
THAWING MODE. Push START when in STANDBY mode to start thawing. The unit then recirculates
tempered water through the BLADDERS, and massages the plasma bags. The countdown timer indicates the
remaining time. The plasma bags are monitored for leaks. Timing out or pushing STOP ends thawing.
DIAGNOSTIC MODE. Is for technician access. Hold MINUS as you turn the unit ON.
1. To restore the unit to initial factory settings, press ENTER while still holding MINUS. The unit displays
“UNIT RESET”. Turn the unit off. You have to re-calibrate the temperature after resetting the unit.
2. “PUMP TEST” is a diagnostic tool to check if each pump turns on. It is checked only when a pump does
not operate. Release MINUS then push ENTER to verify that you are a technician. The display shows
“PUMP TEST”. MINUS turns on Pump 1, ENTER turns on Pump 2. Push PLUS to go to next function.
3. Overtemperature Alarm Test. Hold MINUS as you turn the unit ON. DIAGNOSTICS will be displayed.
Push ENTER. Push and release PLUS until ALARM TEST is displayed. Push ENTER, the heater will
heat continuously and the pumps will turn on. When the temperature reaches 1.0C above the target
temperature the alarm will sound. Turn Off the unit. If alarm does not sound, contact CytoTherm.
Alarm test should be performed every 3 months as part of unit maintenance.
PROGRAM MODE. Allows you to:
1. Program the thawing time.
2. Select whether to continue thawing when timed out.
3. Calibrate the temperature.
4. Set the target thawing temperature. The Alarm temperature is 1.0C above the target temperature.
5. Select the signal when thawing is done (continuous or pulsed).
6. Adjust the massaging timing.
Enter PROGRAM MODE by holding ENTER while turning the unit ON. Follow the displayed instructions to
program. Pushing PLUS advances the menu to the next parameter.

6
Programming the Thawing Time. When the thawing time is displayed, press MINUS because you want to
change the time. Use PLUS or MINUS to set the desired new thawing time, press ENTER to program in the
new time. Turn the unit off.
Calibrating Temperature. Turn the unit on and let it heat up to 37.0
o
C. Check the displayed temperature with
your accurate thermometer. There is a covered THERMOMETER WELL on the left side of the control panel.
Rotate the cover bar to expose the thermometer well. Place your thermometer into the THERMOMETER
WELL. The thermometer stem should be at least 15 cm. (6”) long. If the displayed temperature needs to be
changed, turn the unit off. Enter PROGRAMMING MODE by holding ENTER as you turn the unit on. When
the thawing time is displayed push PLUS until the display shows “CALIBRATE TEMPERATURE”. Push
MINUS because you want to change the displayed temperature. Use PLUS or MINUS to make the displayed
temperature agree with your thermometer. Push ENTER to record the calibration. Turn the unit off. If the
unit can not be calibrated, or if the calibrated temperature is not stable, have a technician check the unit. See
TECHNICIAN FUNCTIONS.
Adjust the massaging. Pump 1 fills the outer tubes of the BLADDER. Pump 2 fills the center tube BLADDER.
Pump 1 is on constantly when thawing, but at a lower pressure. Pump 2 then controls the massaging action:.
1. Pump 2 ON time pressurizes the center tube and pushes the plasma towards the outside tubes.
2. Pump 2 OFF time lowers the pressure of the center tube and allows plasma to be pushed to the center.
You can observe the action and adjust the Pump 2 times to change the massaging action. The preset times
have been tested to work very well.
SWITCH FUNCTIONS
There are 6 switches on the front panel:
START. Starts function.
STOP. Stops function.
ENTER. Records parameter changes.
PLUS. Steps through parameters and increases value of parameter.
MINUS. Allows change in parameter and decreases value of parameter.
Sketch of FIN logo. Not currently used. Future expansion

7
TROUBLESHOOTING
English: Unit should be opened by qualified technicians only.
French: Ne puet être ouvert que un techicien autorize.
Unit is "dead" Verify that you are plugged into a live, grounded outlet.
Have qualified technician check if fuse is blown or connectors are
loose. There are three fuses (see wiring diagram) in the unit. Fuse-1 &
Fuse-2 are located on the circuit board: 8Amp for 120Vac models and
5Amp for 240 Vac models. Fuse-3 is a ¾ Amp slow blow inline fuse
on the transformer 12 Vac line.
Computer does not boot when
turned on. Does not display
readable messages.
Unit is not responding, but the display is backlit. Backlit display
indicates that the unit has power. Turn unit off for at least 10 seconds
and turn unit back on. If this does not solve problem, replace the
computer (program module part# CD4-PM).
Display says “low water” but
there is water.
The unit is not sensing that water is present.
a. Make sure you are using tap water or adding salt to deionized
water to make it conductive.
b. The level sensor (screw inside the BATH on the right side) is dirty.
Scrape it and then clean with alcohol.
Unit does not heat but
"heating" is displayed
Was the unit filled with water over 40C.? If yes then cool the water to
20C to reset the safety thermostat.
Check the Safety thermostat (#C-TH) and the heaters (#14-77) for
continuity.
Bag leaked, but does not
trigger alarm and “LEAK”
message..
Lift front of RACK. Clean the stainless steel nuts exposed on the front
of the RACK with alcohol. Clean the stainless steel springs that
protrude from the BATH with alcohol and bend out the bottom of the
spring so that it is 1.2 cm. (.5”) from the BATH wall. If there is still a
problem, contact CytoTherm.
Temperature calibration does
not “hold”, it changes .
Replace temperature sensor (#CT4-TS).
Temperature calibration is out
of range.
Have technician check the voltage at TP1 (see wiring diagram) , it
should be 0.395 +/- .001 Vdc. If TP1 was correct, replace the
temperature sensor (#CT4-TS).
If TP1 was not correct, restore the unit to initial factory settings (see
DIAGNOSTICS) do full calibration. See Technician Functions > Full
Calibration.
MESSAGES, Troubleshooting
COLD indicates that the water temperature is less than 20.0C. As soon as the temperature rises above 20C, it
will display the true temperature.
TOO HOT is an audible and displayed alarm condition that indicates that the bath temperature is 1.0C higher
than the set temperature. If the operator pours hot water into the bath he/she will trigger this alarm.
This should never happen in normal operation. If it does, contact CytoTherm.
CLOSE LID indicates that the lid is not closed. If the lid is closed, check if there is a magnet imbedded in the
LID on the front right side. If the magnet is there, then the magnetic sensor (reed relay) may have failed. Have
technician check the sensor and its connections. If the sensor and the connections are OK then the PC board
should be replaced.

8
LOW WATER First make sure that a pinch ( 0.01 gr) of salt was added if deionized water was used. Then
remove RACK to add water. Lift up the top layer of the bladders to let the water in the BLADDER drain down
into the BATH. Lift the front of the RACK and disconnect the RACK TUBING. Lift the RACK and put it
aside. Pour water into the BATH to the water label.
LEAK is an audible and written alarm that indicates that a plasma bag or BLADDER leaked.
MAINTENANCE
Daily: Wipe off the unit.
Every 3 months:
Replace the bladders and clean the bath.
1. Unplug the unit.
2. Remove and discard the BLADDERS by first lifting the top of the BLADDERS up to drain out as much of
its internal water as possible. Remove the INLET and OUTLET FITTINGS by holding the FITTINGS
and twisting them as you pull up.
3. Lift the front of the RACK and disconnect the RACK TUBING from the RACK, lift up the Rack and
place aside.
4. Drain the bath by attaching the provided tubing to the DRAIN valve. Most of the water will drain out.
Tilt the bath to drain more of the water.
5. Wipe the BATH and RACK with bleach towelettes or a 10% bleach solution. Use only water and bleach
towelettes or a 10% bleach solutions for cleaning. DO NOT USE CONCENTRATED BLEACH. Use
alcohol for removing oil or grease. Rinse off the bleach with water.
6. Close the DRAIN valve. Fill the bath with water to the water label. Place the RACK on the BATH. Do not
connect the RACK TUBING. Plug the unit in, turn it on and start a thawing cycle. Let the unit run for a
few minutes so that the water passes through and rinses the pumps.
7. Remove the RACK, and drain out the water. Close the DRAIN valve. Fill the bath with water. If you use
deionized or distilled water add a little pinch ( about 0.01 gr.) of table salt. Do not use saline solution.
Add 3 ml. of Algicide Part #ALG240 (1.0 ml. per 4 liter of water available in contiguous USA only) to the
water. Any other bath clearing agent can also be used.
8. Connect the RACK TUBING and position the RACK in place.
9. Mount new BLADDERS in the RACK .
Replace the air filter. Unscrew the filter cover, discard the old filter and mount the new filter. Screw on the
filter cover.
Stainless Steel Springs. The two stainless steel spring rods that push against the rack act as connectors to the
leak sensors in the RACK. Bend them out so that the bottom of the spring is 1.2 cm (.5”) from the BATH wall.
In the very unlikely event that the spring breaks, please contact CytoTherm and we will rush you a new spring
with mounting instructions. The unit will work, but will not detect leaks, with a broken spring until you install
the new spring. Wipe the stainless steel springs and the stainless steel nuts on the front of the RACK with
alcohol to remove any oil or grease.
Leak Test Turn the unit ON. Take a slightly moist piece of a paper towel and touch it to both of the stainless
steel studs inside the front of the RACK. The alarm should sound and the display should say LEAK. If it does
not, contact CytoTherm.
Temperature Calibration. Run temperature calibration at least every 3 months or more often if your
institution procedures or regulations require. See PROGRAM MODE > TEMPERATURE CALIBRATION.
Overtemperature Alarm Test. Hold MINUS as you turn the unit ON. DIAGNOSTICS will be displayed. Push
ENTER. Push and release PLUS until ALARM TEST is displayed. Push ENTER, the heater will heat
continuously and the pumps will turn on. When the temperature reaches 1.0C above the target temperature

9
the alarm will sound. Turn Off the unit. If alarm does not sound, contact CytoTherm. Alarm test should be
performed every 3 months as part of unit maintenance.
BLADDER Maintenance. The bladder does not require periodic maintenance. In case of a plasma bag leak,
remove the BLADDER, rinse the BLADDER under running water and wipe with bleach towelettes or a 10%
bleach solution. Rinse off the bleach.
PARTS LIST CT-D4
CD4-MPL Pump, Magnetic DP-SW Switch, 2-pole
14-77 Heater (2 needed) C-TH Safety Thermostat
CT4-PC Printed Circuit Board CD4-DISP Display
CD4-BTH Bath Section CD4-BL Bladder ( 2 needed)
CD4-RK Rack for Dry Thaw CD4-PM Program Module
C4-TS Temperature Sensor CD4-Y "Y" Accelerator (use 4)
C4-TR Transformer 120V CD4-LID Lid for Rack
CD4-TR2 Transformer 240V C-VLVF Drain Valve Twist Female
C4-FILT Air Filter. Per 4 pack C-VLVM Drain Valve Twist Male
14-77J Heater 100V (Japan only) ALG240 Algicide
CD4-TRJ Transformer Dual (Japan)
Always specify model and serial number when ordering.
SOURCES for SUPPLIES
BAGS overwrap 15 cm. wide by 30 cm. (6” x 12”) long are available from US Plastics 1390 Neubrecht
Rd. Lima OH 45801 Tel. 800 537-9724. Part #47352.
TECHNICIAN FUNCTIONS
FULL CALIBRATION
Adjust the 10-turn potentiometer so that the voltage between TP1 and Ground (Earth) is .395 V (see wiring
diagram for locations). Actual temperature calibration is then set in Programming Mode (see
PROGRAMMING MODE).
The trimmer near the BODY connector adjusts the display contrast.

10
SAFETY FEATURES
WATER LEVEL SENSOR
The unit will display LOW WATER and beep if there is not enough water to cover the level sensor inside the
bath, a screw located 3/4" above the temperature sensor. The heat will also turn off. The water must be
conductive in order for the level sensor to work. Use tap water or add a pinch (0.01 gr.) of salt if you are using
de-ionized or distilled water.
OVER TEMPERATURE
The unit will display TOO HOT and emit double-beeps if the temperature reaches 1.0
o
C above the thawing
temperature. The controls will turn the heaters off. In the unlikely situation that there is a component failure
and the unit continues heating, an independent safety thermostat will turn the heating off at 45
o
C.
SAFETY THERMOSTAT
An independent safety thermostat will turn heaters off if the temperature goes above 45
o
C. It has to be cooled
to 20 C to reset.
FUSING
The main fuses (Fuse-1 & Fuse-2) are located on the Printed Circuit Board and are 8A for 120Vac and 5A for
240Vac AG3 fuses. Correct the cause of the blown fuse.
The transformer has a ¾ Amp slow blow fuse (Fuse-3) on the wires of the 12 Vac winding used to supply the 5
Vdc supply.
ISOLATION
The circuitry is isolated from the power lines by a transformer and from the heaters by an opto-coupled solid
state relay.

11
This page intentionally left blank.

12
Ground is Green with Yellow stripe
Ground
Wire Nut
120V
Wiring CT-D4 & CT-D2
Dwg # 434
Level
Sensor
Screw
Thermostat
Safety
Pump A
Temperature
Sensor
Blk
Heater
Heater
1
1
11
Blu
Orn Orn
120V wired as shown
240V the 2 heaters are in series
Brn
Whi
Grn
Orn
Red
Vio
Switch
Grn
High
Volt
Filter
Brn=High V
Grn/Yel=Gnd
Blu=Neutral
Power Cord
Orn
Blu
Orn
Blu
Grn/Yel
/Yel
Start Stop Minus Plus Enter
Blu
Grn
Gra
2Blu
Vio
2Grn
2Yel
2Orn
2Red
2Brn
&
CT-4D Board
Blk
Blk
Blu
Blu
Brn
Vio
Blk Whi
Blu
Blu
Both Blu wires are Neutral
120V
Pump B
DISPLAY
1
Switches
Buzzer
Chip
Computer
1
Ribbon Cable
Older Unit
Switch
Connector
15 Apr 04 Change dwg to move Switches
Connector to right corner. Change
started with board ver.G.
Add D2
May 2003 New
[H]
[N]
[M]
[X]
[12VAC]
5
1
2
=MOV
120V xform
3
4
8
[H]
[N]
[X]
[12VAC]
4
1
2
240V xform
5
3
8
1
[M]
1
Grn
Blu
Orn
Orn
Grn
Red
Red
Orn
2
2 2
2
= 240V MOV
Blu
Yel
~
Yel
Blu
~
Rev 20 June 06. Fuse table & TP1
Rev 5 June 04 add 3/4A slow blow fuse
to 12 Vac Xform lead. See D 467.
TP1
+
GND
Fuse-1
Fuse-2
Fuse-3
Fuse-3
Fuse Amps Vac Type
1&2 8 120 Fast
1&2 5 240 Fast
3 3/4 12 Slow-Blow
Table of contents
Other CytoTherm Laboratory Equipment manuals
Popular Laboratory Equipment manuals by other brands

3D Histech
3D Histech Pannoramic 250 FLASH II user guide

PerkinElmer
PerkinElmer Spectrum RX I Getting started guide

Bio-Tek
Bio-Tek MultiFlo FX Operator's manual

Exor
Exor X5 quick start guide

Bio-Science
Bio-Science Ampulmatic-10 Purge Gas Injector Operation and maintenance manual

VWR
VWR Peqlab PerfectBlue Mini S instruction manual