Dart SVP-3DS Installation instructions
DART 3+D Scanner
Assembly Instructions and User Manual
For SVP-3DS
Version 2.0
IonSense Inc.
999 Broadway
Suite 404
Saugus, MA 01906
2
This manual details the steps necessary to install and use the 3+D scanner SVP-
3DS for the DART SVP.
Contents
HARDWARE ...................................................................................3
K
EY OF
3D
S
CANNER
P
ARTS
........................................................................ 3
3D
S
CANNER
A
SSEMBLY
...................................................................... 4
SOFTWARE ................................................................................... 10
M
ETHODS
........................................................................................... 11
3+D Imaging ................................................................................... 12
3+D Scanner Glass Inserts .............................................................. 14
3
Hardware
Key of 3D Scanner Parts
1. 300mm Linear Rail (X-Rail)
2. 100mm Linear Rail (Y-Rail)
3. X-Rail Cradle (DART-SVP Source Rev 1 ONLY)
4. 3D Sampling Stage
5. Y-Rail Cradle
6. Allen Wrenches (1.5mm, 2 mm, 3mm)
1
2
3
5
4
6
4
3D Scanner Assembly
DART-SVP Source Rev 1: (SN: SVPS00XXX)
Figure 2: Placing the X-Rail into the Cradle
Using the 3mm Allen wrench, remove the three screws that attach the X-cradle to the base of the DART
SVP. Take the X-Rail and slide the non-motor end (end opposite the cable) into the cradle, making sure
that the front of the stage faces the screw holes.
Reference the Hardware and Network Installation Manual v2 manual to determine the exact placement of
the rail in the cradle as this procedure depends on the version and revision of the rail.
Using the 1.5mm Allen wrench, lock the rail into position by tightening the two set screws on the front of
the cradle. One the rail is placed back onto the DART these set screws will be inaccessible.
Figure 3: Tighten the set screws to lock the X-Rail into position.
Front of Stage Facing away
fro MS/Towards Cradle Screws
5
DART-SVP Source Rev 2: (SN: SVPS002XX)
The revision 2 DART-SVP source, pictured below, has an X-Rail Cradle but it is not removable from the
source baseplate. Simply place the linear rail directly into the cradle on the baseplate, and lock into place
using the two set screws.
As with the Rev 1 source, reference the Hardware and Network Installation Manual v2 to determine the
exact placement of the rail in the cradle.
Y-RAIL Installation:
Repeat the process to attach the Y-Rail to its cradle. Slide the non-motor end of the rail into the
housing, leaving it so the end of the rail is flush with the end of the cradle. Again, using the
1.5mm Allen wrench, tighten the set screws to lock the rail in place.
Figure 4, a,b, and c: the Y-Rail with its cradle; the Y-Rail in its cradle; locking the Y-Rail in place.
6
Place the block end of the Y-Rail on top of the X-Rail’s stage. Using four 2.5mm screws and the
2.5mm Allen wrench, secure the block onto the stage, aligning the Y-Rail so that the front piece
of the Y-rail’s stage faces the cradle. Tighten the two 1.5mm set screws on the back of the block
to finish attachment.
Figure 5: Showing the alignment of the X- and Y-Rails before they are connected.
Figure 6: Secure the Y-Rail to the X- using a 2.5mm Allen wrench.
7
Pull the DART SVP source housing all of the way back from the mass spectrometer inlet and
remove the ceramic tube from the VAPUR interface. Supporting the rail assembly carefully,
place the cradle back into the space between the MS interface and DART, with the three screws
on the far side of the MS.
Figure 7: The rail assembly attached to the DART source.
Figure 8: Securing the rail assembly to the DART base with the 3mm Allen wrench.
8
DART-SVP Source Rev 1: Loosen the two knobs on the back of the pillars holding the blue DART
source. Raise the DART source to its maximum height so that the 3+D sample tray can fit underneath it.
Loosen the knobs on the side of the DART and turn it downward three clicks so that it is now pointed
downward at a 45 degree angle. Tighten the knobs to lock it back into place.
Figure 9a and b: Rev 1 Source at maximum height and 45 degree angle
DART-SVP Source Rev 2: Attach the SVP-45A angle module to the DART source housing. Use the
spinning knob to adjust to the proper height.
Figure 10: Rev 2 Source with SVP-45A angle module
9
Place the block of the sample tray on the Y-Rail stage and secure the top of the block using four 2.5mm
hex screws. Then, using a 1.5mm Allen wrench, tighten the two set screws on the back side of the rail.
This will hold the sample tray firmly in position.
Figure 11: Securing the Stage to the Y-Rail
Insert the longer ceramic tube into the VAPUR inlet, taking care to leave a 2mm gap between the end of
the ceramic tube and the API inlet.
Refer to the specific Vapur Flange Manual for the mass spec instrument being installed on for more
details.
10
Software
Check the About page of the SVP software to see the version of software currently loaded. For this
manual v3.x.x or higher should be uploaded.
The most up to date version of software can be downloaded from the IonSense website at this
address: http://www.ionsense.co /software
If the software needs to be updated then download the file from the IonSense website and
reference the DART Software Manual v3 for instructions on how to update. A printed version
of this software manual comes with each DART system, or can be access here:
http://www.ionsense.co / anuals
Web Software: Page 19
iPod Software: Page 20
11
Methods
Once the software is updated select the Methods tab at the bottom of the software screen. There
will be two new methods available.
NOTE: The methods may need to be added to the main list; reference page 8 of the Software
Manual v3 for details.
•3+D Imaging
This is the standard/generic method for the 3+D scanner. It performs a basic back and
forth scan of a 2D surface.
•3+D Scanner Glass Inserts (L or R)
This method performs the same basic scan, but designed specifically for our glass insert
plate. These inserts support liquid sampling. There is both a left and right version of the
software depending on the linear rail setup.
Before running the method, be sure the Linear Rail settings are correct. If they aren’t the
methods won’t run properly. To check, open the Settings tab in the software; open both Linear
Rail #1 (X) and Linear Rail #2 (Y). A left handed setup should have left selected for both Motor
Location and Sample Direction and vice versa for a right handed setup. Below are the correct
settings for a left handed rail setup.
12
Once the method is selected either hit Start to begin the method, or select Edit to change the
various parameters. Each of these methods is described in more detail on the following pages.
13
3+D Imaging
The following parameters can be adjusted:
Sample Count
Number of rows.
Ion Mode
Ions can be positive or negative.
Temperature
The temperature of the heater.
X Start Position
X location where the sample starts.
X Sample Speed
How fast the X rail moves over the sample.
X Length
Length of the sample.
Y Start Position
Y location where the sample starts.
Y Sample Speed
How fast the Y rail moves over the sample.
Y Spacing
Distance between rows.
Shutdown State
The system can be kept in run or standby mode for repeated use or automatically shut down.
Standby Temperature
If the system is kept in run or standby mode, this temperature will be maintained.
14
3+D Scanner Glass Inserts
The 3+D scanner comes with 96-well plates and circular glass inserts. The inserts should be
placed in the plate so that the closed ends face upward. The closed end is a concave surface and
each one can hold up to 12uL of liquid. Liquid samples can then be spotted onto the surface
using a micro pipettor or liquid handling robot. (IonSense does not support the robotic option.)
Each plate supports up to 48 samples, and the placement of the inserts depends on the
configuration of the 3+D scanner, left or right handed. These configurations allow for the
DART-SVP to be mounted on many instruments.
•For Left Handed Rails
In this configuration, the sample plate will be introduced from the left side of the DART
Source. Due to the heat from the DART gun, samples cannot be placed on every row.
Instead, samples should be placed in every other row, starting with row A. The plate
should be mounted with A1 positioned in the bottom right corner, near the DART Source.
15
•For Right Handed Rails
In this configuration, the sample plate will be introduced from the left side of the DART
Source. Due to the heat from the DART gun, samples cannot be placed on every row.
Instead, samples should be placed in every other row, starting with row B. The plate should
be mounted with A1 positioned in the bottom right corner, away from the DART Source.
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Just as with the previous method, there are a variety of parameters that can be adjusted.
Editable Parameters
Sample Count
Up to 48 sa ples can be run at a ti e.
Ion Mode
Ions can be positive or negative.
Start Temperature
The te perature of the heater while
running the ethod.
Gap Time
How long the ethod waits in between
sa ples, leaving a gap between spectra
peaks.
Sample Time
The nu ber of seconds the sa ple stays
in the DART bea .
Shutdown State
The syste can be kept in run or standby
ode for repeated use or auto atically
shut down.
Standby Temperature
If the syste is kept in run or standby ode, this te perature will be aintained.
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