Denville Scientific XL3000I XL8-10 User manual

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CONTENTS
1 - INTRODUCTION
2 - VOLUME SETTING
3 - METHOD OF PIPETTING
4 - RECOMMENDATIONS
5 - RECALIBRATION
6 - MAINTENANCE
7 - PIPETTE KIT AND ACCESSORIES
8 - SPARE PARTS
1 - INTRODUCTION
Multichannel p pettes have been
des gned for use w th m croplates. The p pettes
enable prec se
and s multaneous del very of 8
or 12 preset-volumes of l qu d.
Multichannel p pettes are produced n four
ranges of volumes: 1-10 µl, 5-50 µl, 20-200 µl,
50-300 µl.
Multichannel p pettes are
equ pped w th a d g tal readout counter, bu lt nto
the p pette’s handle,
for measur ng volumes,
(F g. 1F). Volume sett ngs are cont nuously
adjustable and are controlled by rotat ng the
cal brat on knob, (F g 1B). The volume range of
the
p pette s nd cated on the p pett ng push-
button, (F g. 1A).
Multichannel p pettes should be
used w th polypropylene d sposable t ps, to
ensure safety and accuracy, (F g. 1C). The
shafts ensure the compat b l ty of the
p pettes
w th a broad assortment of t ps, and the spec al
ejector shape reduces the force requ red for
the r eject on. In order to protect the user aga nst
nadvertent contact w th used t ps, the
p pettes
are equ pped w th t p ejectors, (F g. 1D). By
turn ng the mult channel module clockw se n
1 2
relat on to the handle, (F g. 2), t s poss ble to
d sable the ejector’s operat on n order to avo d
acc dental use, or to change ts pos t on n such
a way as to make f ll ng of m croplates more
conven ent.
The spec f cat ons for accuracy and prec s on
g ven n the follow ng table are obta ned us ng
t ps. These f gures are only
guaranteed when t ps are used.
8 channel
12 channel
The spec f cat ons for the volumetr c perfor-
mance are der ved from extens ve grav metr c
tests as prescr bed by the Deutsches Inst tut für
Normung e.V. To ver fy the performance of your
p pette request document DIN 12650.
Cond t on of measurments: d st lled water at tem -
perature 20±1°C.
The
p pette des gn enables the user to perform
the recal brat on process accord ng to the
gu del nes presented n sect on 5.
XL8-10 Min 1 ±8.0 ≤ 8.0
Max 10 ±2.0 ≤ 2.0
XL8-50 Min 5 ±4.0 ≤ 3.0
Max 50 ±1.6 ≤ 1.0
XL8-200 Min 20 ±2.0 ≤ 1.5
Max 200 ±1.0 ≤ 0.8
XL8-300 Min 50 ±1.6 ≤ 1.5
Max 300 ±1.0 ≤ 0.8
Model No. Volume Inaccuracy Im recision
[µl] [%] [%]
Model No. Volume Inaccuracy Im recision
[µl] [%] [%]
SPECIFICATIONS
XL12-50 Min 5 ±4.0 ≤ 3.0
Max 50 ±1.6 ≤ 1.0
XL12-200 Min 20 ±2.0 ≤ 1.5
Max 200 ±1.0 ≤ 0.8
XL12-300 Min 50 ±1.6 ≤ 1.5
Max 300 ±1.0 ≤ 0.8
SPECIFICATIONS
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2 - SETTING THE VOLUME
In order to set the volume of the sample to be
del vered, the cal brat on knob w th d splay s
used.
The volume shown by the d splay s repre-
sented by three d g ts, wh ch should be read
from top to bottom. Typ cal meter read ngs are
shown n the follow ng table:
To ach eve accurate del very of the requ red
volume, the operator should turn the cal brat on
knob n the d rect on d m n sh ng d splay rea-
d ngs. If the volume be ng currently set s h gher
than the prev ous one, the operator should turn
the cal brat on knob 1/3 of a turn beyond the
requ red value, and then slowly rotate t back-
wards, observ ng d m n sh ng read ngs, unt l the
requ red value s ach eved.
3 - METHOD OF PIPETTING
F t the t ps onto the cones of the mult channel
module. When you put the t ps on the shafts the
p pette should be pressed aga nst the t ps n the
box. The l qu d asp rated nto the t ps should
not flow out by grav ty from properly f xed t ps.
Wh le hold ng the
p pette n a vert cal pos t on,
t ps should be mmersed n the l qu d to a depth
of 2-4 mm, and flushed once by draw ng a volume
of l qu d and d spens ng t out w th slow and
steady movement.
Model No.
Counter
readings
Set
volume
Basic
increment
0
3
5
2
5
0
0
8
5
0
6
5
XL8-10
XL8-50
XL12-50
XL8-200
XL12-200
XL8-300
XL12-300
3.5 µl
6.5 µl
85.0 µl
250.0 µl
0.02 µl
0.10 µl
0.20 µl
1.0 µl
3 4
Next, st ll hold ng the p pette vert cally, the ope-
rator should press the p pett ng button unt l the
f rst res stance po nt s felt, and mmerse the t ps
n the l qu d to the depth of 2-4 mm, (F g. 3B).
Releas ng the p pett ng button w th a slow and
un form movement dur ng 2-3 seconds, the l qu d
should be drawn nto the t ps, (F g. 3B) wh ch
should then be l fted above the l qu d’s surface.
The
p pette should next be pos t oned at an
angle of 10-45 degrees n relat on to nner walls
of the dest nat on vessels and t ps should be
empt ed by press ng the p pett ng button
slowly unt l the f rst res stance po nt s felt,
(F g. 3C). After wa t ng one second, the p pett ng
button should be pressed to the second res s-
tance po nt n order to d spense rema n ng l qu d,
(F g. 3D). Then t ps should be l fted out from the
vessels wh le ma nta n ng contact between
the ends of the t ps and the nner walls of the
vessel unt l the p pett ng button has been
released, (F g. 3E). F nally, press ng the ejector
button, the operator should separate the t ps
from the mult channel module’s cones, (F g. 3F).
- RECOMMENDATIONS
To ach eve max mum safety, prec s on and re-
l ab l ty, the follow ng pr nc ples should be ob-
served:
• do not draw l qu ds w thout t ps f tted on the
p pette cones,
• do not lay the
p pette flat w th t ps f lled,
• do not draw volumes of l qu d exceed ng the
p pette ’s range,
• check f the t ps are f tted properly,
•dur ng operat on, the
p pette must be held
vert cally, t ps should be mmersed n l qu d
to the depth of 2 to 4 m ll meters and the p pet-
t ng button should be depressed and released
slowly and evenly,
• new t ps must be re-wetted pr or to p pett ng,
by draw ng and expell ng the l qu d to be me-
asured. Th s s espec ally mportant when wor-
k ng w th l qu ds of v scos t es and dens t es
d fferent from that of water and n temperatu-
res other than amb ent temperature,
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The recalibration can be performed within one
full turn of the key to the right or to the left only.
Recalibration conditions:
• Amb ent temperature and the temperature of
the p pette, t ps and l qu d should be w th n
the range 20-25°C and stab l zed dur ng we gh-
ng
w th n ±0.5 ° C
•Measurements should be conducted us ng
d st lled water
• Balance sens t v ty should be su table for the
volume to be controlled
Recalibration procedure:
• Set the volume depend ng on the p pette volu-
me accord ng to the follow ng table:
•Perform three asp rat on ser es (each ser es
should nclude the asp rat ons from all chan-
nels), we gh each t me and calculate the aver-
age value of the asp rat ons.
• Calculate average asp rated volume n µl mul-
t ply ng the average asp rat on amount [mg]
by the d st lled water dens ty coeff c ent
[µl/mg], wh ch depends on temperature and
pressure accord ng to the follow ng table:
5
• when p pet ng l qu ds wh ch tend to wet walls
of t ps, such as serums, prote ns, organ c sol-
vents, measur ng must be performed much
slower than w th other l qu ds,
•
t ps must be replaced w th new ones, when
chang ng from one l qu d to another, or f drops
of l qu d rema n ns de the t ps,
• after use, the p pette should be stored vert cal-
ly n a holder w th t ps removed.
5 - RECALIBRATION
Multichannel p pettes are cal brated
by grav metr c method, us ng d st lled water, at
the temperature 20±1°C, accord ng to DIN
12650.
If dur ng p pette operat on you f nd that the
accuracy error (the d fference between the real
asp rated volume and the preset volume)
exceeds the perm ss ble value g ven n the table
n sect on 1, the p pette recal brat on procedure
should be carr ed out.
Before start ng the recal brat on t s necessary
to check whether the follow ng requ rements
have been fulf lled dur ng error determ nat on:
• the amb ent temperature, and the temperature
of the p pette, t ps and water was dent cal
• the dens ty of the l qu d used s close to that
of d st lled water
•the balance w th appropr ate sens t v ty has
been used
• mg/µl convers on factor has been taken nto
account
•the requ rements g ven n sect ons 3 and 4
have been fulf lled
If the above cond t ons are sat sf ed and the accu-
racy error for selected volume g ven n sect on 1
exceeds the perm ss ble value, the p pette
recal brat on procedure should be carr ed out.
Volume checked [µl]
0.1 - 10
10 - 100
> 100
Balance sensitivity [mg]
≤ 0.001
≤ 0.01
≤ 0.1
Temperature [°C] Pressure [hPa]
960 1013 1067
20 1.0028 1.0029 1.0029
21 1.0031 1.0032 1.0032
22 1.0032 1.0033 1.0033
23 1.0035 1.0035 1.0036
24 1.0037 1.0038 1.0038
25 1.0039 1.0040 1.0041
1 - 10
5 - 50
20 - 200
50 - 300
1
5
20
50
0.92 - 1.08
4.8 - 5.2
19.6 - 20.4
49.2 - 50.8
0.33
1.67
6.30
10.00
Range
of the i ette
volumes [µl]
Preset
volume
[µl]
Permissible
volumes
[µl]
Volume change ∆V for
full turn of the calibra-
tion key [µl]
(24 increments)
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If the average asp rated volume exceeds the
perm ss ble value, the follow ng should be done:
• Remove the p pett ng pushbutton, (F g. 4A)
•Hold ng the volume sett ng knob to protect
t aga nst rotat on, nsert the cal brat on key
nto the cuts of the cal brat on screw, (F g. 4B)
• Turn the key clockw se to reduce the asp rat-
ed volume, or counter-clockw se to ncrease
the volume, (F g. 4C). One full turn of the cal bra-
t on key changes the p pette asp rat on volume
by the amount g ven n the table
•Take out the key and replace the p pett ng
pushbutton, (F g. 4D).
Determ ne the average asp rated volume. The
average volume should be w th n the perm ss -
ble range g ven n the table. If the volume
exceeds the values stated, the recal brat on pro-
cedure should be repeated.
6 - MAINTENANCE
Cones should be kept clean us ng ethyl alcohol.
7 - PIPETTE KIT
AND ACCESSORIES (OPTIONAL)
Pipette kit
The p pettes are del vered n the k ts nclud ng:
• P pette
• Instruct on manual
• Cal brat on key
• Ident f cat on labels
Accessories (Opt onal)
Pipette Tip Choices
10 µl, 10 racks of 96 (960 ti s er ackage)
XL8-10
200 µl, 10 racks of 96 (960 ti s er ackage)
XL8-50
XL8-200
XL12-50
XL12-200
300 µl, 10 racks of 96 (960 ti s er ackage)
XL8-300
XL12-300
Pipette Stand Choices
Stand for eight channel i ette
XL8
Stand for twelve channel i ette
XL12
7 8
8 - SPARE PARTS
The spare parts for mult channel, (F g. 1, F g. 4):
1. Pushbutton
2. Ejector pushbutton
3. Calibration knob
4. Ejector
5. Piston assembly
6. Calibration key
can be ordered from Denville (type of the p pette
and name of the part for th s p pette should be
spec f ed).
Before return ng a p pette to Denville please
ensure that the p pette s completely free of any
contam nat on (chem cal, m crob al or rad o-
act ve).
Warning: The replacement of the plunger
requires conducting of calibration proce-
dure according to section 5.
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