DLAB Laboratory Instruments D3024 User manual

D3024 & D3024R
High Speed (Refrigerated) Micro-Centrifuge
Dragon Laboratory Instruments Limited
www.dragon-lab.com
Before using this centrifuge, please carefully read this user manual for its efficient operation and
your safety.

High Speed (Refrigerated) Micro-Centrifuge
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Contents
Copyright…….………………………………………………………………………………….….….… 2
Safety reminder…….…………………………………………………………………………………..…3
1. Specifications…………………………………………………………………………………………...6
2. Declaration of Conformity…………………………………………………………………………..…6
3. Required operational conditions………………………………………………………………….…....7
3.1 Basic operational conditions…………………………………………………………………………..…7
3.2 Transport and storage conditions………………………………………………………………….….... 7
4. Installation……………………………………………………………………………………………..…7
4.1 Location…………………………………………………………………………………………….……7
4.2 Connection of the power cord and grounding……………………………………………………….…..8
5. Structure…………………………………………………………………………………………….……8
6. Operation panel…………………………………………………………………………………….… 10
7. Rotor preparation……………………………………………………………………………….…….12
7.1 Prepare the sample……………………………………………………………………………………12
7.2 Inject the sample into the tubes……………………………………………………………………….12
7.3 Keep the tubes balance………………………………………………………………………………..12
7.4 Inspect the rotor……………………………………………………………………………………….12
7.5 Symmetrically load centrifuge tubes in rotor……………………………………………………….…13
8. Operation………………………………………………………………………………………………..13
8.1 Normal operation…………………………………………………………………………………….…13
8.2 RCF operation………………………………………………………………………………………..…17
8.3 Pulse operation………………………………………………………………………………………….17
9. Maintenance………………………………………………………………... ………………... ….……18
9.1 Cleaning…………………………………………………………………………………….………..…18
9.2 Consumables……………………………………………………………………………………………19
9.3 The replacement of seal ring …………………………………………………………………………20
9.4 Routine inspection……………………………………………………………………………………20
10. Troubleshooting……………………………………………………………………….…………….…21
10.1 Frequent problems list……………………………………………………………………………...…22
10.2 How to open the door…………………………………………………………………………….......22
10.3 Replace fuses…………………………………………………………………………………………22
11. Instructions of rotor and tube…………………………………………………………………….… 22
11.1 Rotor instructions………………………………………………………………………………..……22
11.2 Tubes…………………………………………………………………………………………….……23

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12. Calculate RCF………………………………………………………. ………………………….….…25
13. Ordering information …………………………………………………………………………..……25
14. Guarantee.………………………………………………………………………………….….………26
14.1 Guarantee of centrifuge……………………………………………………………………….………26
14.2 Guarantee of the rotor…………………………………………………………………….………..…26
After-sales service……………………………………………………………………………….…...…....26
Copyright:
No part of this manual may be reproduced or transmitted without prior written permission of
DRAGONLAB.
We can not be responsible to inform at real-time if the outline and specifications of centrifuge is subject to
change for improvement.
VERSION201012

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Safety Reminder
Common safety precautions
Carefully read the following safety precautions for a thorough understanding.
zFollow the instructions and procedures described in this manual to operate this centrifuge safely.
zCarefully read all safety messages in this manual and the safety instructions on the instrument.
zSafety messages are labeled as indicated below. They are in combination with signal words of
“WARNING” and “CAUTION” with the safety alert symbol to call your attention to items or
operations that could be dangerous to you or other persons using this instrument. The definitions
of signal words are as follows:
WARNI N G ：Personal Danger
Warning notes indicate any condition or practice, which if not strictly observed, could result in
personal injury or possible death.
CAUTION：Possible damage to instrument
Caution notes indicate any condition or practice, which if not strictly observed or remedied,
could result in damage or destruction of the instrument.
NOTE：Notes indicate an area or subject of special merit, emphasizing either the product’s
capability or common errors in operation or maintenance.
zDo not operate this centrifuge in any manner not described in this User Manual. When in doubt
or have any troubles with this centrifuge, ASK FOR HELP.
zThe precautions described in this User Manual are carefully developed in an attempt to cover all
the possible risks. However, it is also important that you are alert for unexpected incidents. Be
carefully operating this centrifuge.

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WA R N I N G ：
zThis centrifuge is not explosion-proof. Never use explosive or flammable samples.
zDo not install the centrifuge in or near places where inflammable gases are generated
or chemicals are stored.
zDo not place dangerous material within 30cm around the centrifuge.
zMake sure to prepare necessary safety measures before using samples that are toxic,
radioactive or contaminated with pathogenic micro-organisms at your own responsibility.
zIf the instrument, rotor and accessories that has been contaminated by solutions with
toxic, radioactive or pathogenic materials, clean it according to the decontamination
procedure that you are specified.
zIf you require services at site, please sterilize and decontaminate centrifuge in
advance, and then notice the service center involved in the details of the particular
materials.
zDo not handle the power cord or turn on or off the POWER switch with wet hands to
void electrical shocks.
zFor safety purposes, do not enter within 30cm around this centrifuge while it is in
operation.
zWhile the rotor is rotating, never forcedly release the door lock.
zUnauthorized repairs, disassembly, and other services to the centrifuge except by our
service center are strictly prohibited.
CAUTION
zThis centrifuge must be located on one firm and level table.
zMake sure the centrifuge is horizontal before running.
zMake sure the angle between the door and cover is greater than 70 degrees when
open the door.
zBe careful not put your fingers or hands between the door and cover when the door
off.
zDo not move or relocate this centrifuge while it is running.
zWhen there is fluid in the rotor chamber, please promptly dry with a dry cloth to
avoid sample contamination.

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zEnsure to remove any objects and fragments of the tubes dropped inside the rotor
chamber before running this centrifuge.
zCautions on rotors
1）Always check for corrosion and damages on the rotor surface before using it.
Do not use the rotor or bucket if such abnormality found.
2）Do not set the centrifuge speed override the allowable minimum speed of the
rotor kits (rotor, bucket, or the other adapter). Make sure to run it below the
allowable minimum speed.
3）Do not exceed the allowable imbalance.
4）Use the rotor and tubes within their actual capacities.
5）If the rotor is provided with a cap, ensure it is tightened before the operation.
zIf any abnormal condition occurs during operation, please stop it immediately and
contact our service center. Notice the service the alarm code if displayed.
zEarthquake damage is likely to the centrifuge, contact our service center if
abnormality observed.

1.Specifications
Maximum speed 15000rpm(200-15000rpm), increment: 10rpm
Maximum RCF 21380×g, increment: 10×g
Maximum capacity 2ml×24, 0.5ml×36, 4-PCR8 serial tubes
Temperature range -20℃-40℃(D3024R)
Timer 30seconds -99minutes-HOLD, continuous operation
Driving Motor Brushless DC motor
Safety devices
Dual door interlock、Over-speed detector、
Over-temperature detector、Automatic internal
diagnosis
Power requirements
D3024:
Single-phase, 220V-240V, 50Hz/60Hz, 5A.
110V-120V, 50Hz/60Hz, 5A
D3024R:
Single-phase, 220V-240V, 50Hz/60Hz, 10A.
110V-120V, 50Hz/60Hz, 10A
Dimensions（mm）
D3024:（L）280×（W）364×（H）266
D3024R:（L）338×（W）580×（H）324
Weight
D3024: 12kg
D3024R: 30kg
Additional features
Speed/RCF switch、Pulse operation、Processing
display、Voice reminder
2
2. . D De ec cl la ar ra at ti io on n o of f C Co on nf fo or rm mi it ty y
Construction in accordance with the following safety standards:
EN 61010-1
EN 61010-2-10
Construction in accordance with the following EMC standards:
EN 61326-1/ FCC Part 15 Subpart B/ IECS 001
Associated EU guidelines:
EMC-guidelines: 2004/108/EC
Instrument guidelines: 2006/95/EC
This ISM device complies with Canadian ICES-001.

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3. Required Operational Conditions
3.1 Basic operational conditions
（1）Power:
D3024: Single-phase, 220V-240V, 50Hz/60Hz, 5A; 110V-120V, 50Hz/60Hz, 5A
D3024R: Single-phase, 220V-240V, 50Hz/60Hz, 10A; 110V-120V, 50Hz/60Hz, 10A
（2）Ambient temperature: 2℃-40℃.
（3）Relative humidity: ≤80%.
（4）No vibration and airflow around.
（5）No electric dust, explosive and corrosive gases around.
3.2 Transport and storage conditions
（1）Storage temperature: -40℃-55℃.
（2）Relative humidity: ≤93%.
4. Installation
This section describes the instructions that you should abide when install the centrifuge to ensure
your safety and the optimum performance. Before moving the centrifuge, the rotor must be removed.
WARNI N G ：
●Incorrect power supply may damage centrifuge；
●Make sure the power source conforms to the required power supply before
connecting.
4.1 Location
（1）Locate this centrifuge on a firm、flat and level table, ensure the four feet of this centrifuge stand on
the table firmly. Avoid installing on the slippery table-board that conveys vibration.
（2）Ideal ambient temperature is 20℃±5℃, avoid placing the centrifuge in direct sunlight if temperature
exceeds 30 .℃
（3）Ensure the centrifuge has at least 10cm of free space on both sides and at least 30cm behind it to
guarantee cooling efficiency.
（4）No heat or water resource nearby, otherwise easily cause sample temperature increase or centrifuge

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failures.
4.2 Connection of the power cord and grounding
WARNI N G ：
●Do not touch the power cord with wet hands to avoid electrical shocks.
●This centrifuge must be grounded properly.
（1）This centrifuge is equipped with a 3P flat plug, grounding can be done just by plugging the 3P plug
into the outlet.
（2）An over 10A outlet which has a good ground is required, and meet with local safety requirements
to ensure its earth terminal is properly.
5.Structure
D3024
Figure 5.1 D3024 Front view of centrifuge
Seal ring of door
Operation panel
Door lock hook
POWER switch
Cover

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Figure 5.2 D3024 Rear view of centrifuge
D3024R
Figure 5.3 D3024R Front view of centrifuge
Outlet
Seal ring of door
Operation panel
Door lock hook
POWER switch
Cover
Outlet
Air vents
View
Door

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Figure 5.4 D3024R Rear view of centrifuge
6.Operationpanel
Air vents
View
Door
（1）
（2）
（4）（3）
Speed display area Temperature display area Time display area
（5）
15000 25 ℃12min
rpm

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Figure 6-1 Operation Panel
Item Symbol Name Function
1
Pulse button When the door closed, press and hold the button to
accelerate running, release the button to stop it.
2
Open/ lock button Press the button to open the door The button is not
available when the centrifuge is running.
3
Select button Press the button to choose the parameter which you want to
modify.
4 Start/ Stop button
Press down the button to start running. The centrifuge will
brake to stop running if press the button during
centrifugation.
5 Parameter button
Clockwise rotate to increase parameter values.
counter-clockwise rotate to decrease parameter values.
Press down the button, shift between speed and RCF
display.
Figure 6-2 The main interface (D3024)
Main interface of D3024 model is as figure 6-2. The speed is set to be 15000rpm, temperature of
centrifugal chamber is 25 ,℃and the running time is12 minutes. When speed symbol is rotating,
indicating the centrifuge is running, the rotation is faster, the speed is higher. Temperature only displays the
temperature of chamber and can not be controlled. Time symbol displays the ratio of working to time
setting. The total time setting is divided into 10 scales.
15000 25 ℃12min
rpm

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Figure 6-3 The main interface (D3024R)
Main interface of D3024R model is as figure 6-3. The speed is set to be 15000rpm, temperature of
centrifugal chamber is 4 ,℃and the running time is 12 minutes. When speed symbol is rotating,
indicating the centrifuge is running, the rotation is faster, the speed is higher. When temperature symbol
is blinking, indicating refrigeration is running, no blinking means refrigeration stop working.
Time symbol displays the ratio of working to time setting. The total time setting is divided into 10
scales.
7.RotorPreparation
7.1 Prepare the sample
7.2 Inject the sample into tubes.
CAUTION: Do not inject overloading samples into the centrifuge which will be
caused leaking.
Do not exceed the actual capacity allowed in the user manual.
7.3 Keep the tubes balance
Although the centrifuge can accept sample balancing by eye, we recommend that you use this
centrifuge in a well-balanced condition to extend its life expectancy.
Never intentionally run the centrifuge under unbalanced condition even though the allowable
imbalance is not exceeded.
7.4 Inspect the rotor
15000 4 ℃12min
rpm

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CAUTION:
●Any abnormality such as corrosion or scratches are found, stop using the rotor and
contact our service center.
●Only manufacturer’s rotors which claimed can be used with the unit.
Check the rotor and bucket for corrosion or scratch before using.
7.5 Symmetrically load centrifuge tubes in rotor
CAUTION:
zMake sure the rotor door is securely fixed on the rotor, rotor and shaft are tightened.
Otherwise, the rotor may be moved off while rotating and cause damage of the
centrifuge or rotor.
zFirmly tighten the rotor door with rotor.
8.Operation
8.1 Normal operation
Turn on the POWER switch, centrifuge will start the self-checking process. (See figure 8-1)
Figure 8-1 Self-checking interface
CAUTION:
●Do not push or lean against the centrifuge while it is running.
●Do not run the centrifuge, when fragment or sample solution left in the centrifugal
chamber. Always keep the centrifugal chamber clean.
●If this centrifuge makes strange noise during operation, stop it immediately and contact
our service center. Notify the alarm code if displayed.
HELLO 88 ℃88min

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After self-checking, instrument will display accumulative running time, see figure below:
Figure 8-2 Accumulative running time interface
The figure indicates the instrument has accumulated running time 312 hours 56 minutes and 45
seconds, and then the instrument displays the last running parameters.
Figure
8-3 Last running interface
Speed: 15000rpm; running time: 12minutes; centrifugal chamber temperature: 25 ℃
Release the door.
8.1.1 Load and replace the rotor
Figure8-4Loadtherotor
00312 56 45
15000 25 ℃12min
rpm
nut
cover

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CAUTION:
zLoad the rotor on the shaft to ensure rotor is in position until it contacts with the shaft,
and tighten the screw to connect firmly the rotor with shaft, otherwise the rotor maybe
move off to damage the centrifuge.
zShould be confirmed the rotor lid is firmly tightened with rotor.
Load the rotor to the shaft to ensure rotor is in position until it fit well with the shaft.
You should feel a click when the rotor is properly loaded on the shaft. If not, may be something
stuck between the rotor and the shaft. Double check and clean it if necessary.
Rotate the rotor slightly by your fingers to double check, if the rotor vibrates obviously, load the
rotor again.
Clockwise rotate the nut using the wrench to tighten the rotor with shaft firmly.
Close the rotor lid, clockwise rotate and firmly tighten the cover with rotor and ensure is in
position. Close the door and then start running.
The method of removing rotor is as same as the above mentioned by turning the screw
counterclockwise.
8.1.2 Set the operation parameters
Press the button to select required parameters. The parameter can be modified when the
parameter is flashing. Clockwise rotate the parameter button to increase parameter value.
counter-clockwise rotate the parameter button to decrease parameter value. Parameter button rotate
faster, parameter value increase faster. The minimum speed increment is 100 rpm, the minimum time
increment is 1 second.
（1）Set the speed
Press the select button , choose the speed unit rpm, showing the speed parameter.
While select speed button, the speed symbol will flash into the speed parameter input
status.
The minimum speed value you can set 200rpm, the minimum increment is 10rpm.
Clockwise rotate parameter button to increase parameter value. counter-clockwise
rotate parameter button to decrease parameter value.
You can quick set value by parameter button , quickly rotate parameter button to

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speed up parameter changing.
There is a circulating function to increase/ decrease parameters. Clockwise rotate
parameter button , values circulate from small →large →maximum →minimum.
counter-clockwise rotate parameter button , values circulate from large →small →
minimum →maximum.
（2）Set the time:
Press select button , time value flash into time setting mode.
Rotate parameter button to set running time range from 30 seconds to 99 minutes.
When time displays as HD, that showing long-running mode.
（3）Set the temperature (D3024R):
Press select button , temperature value flash into temperature setting mode.
Rotate parameter button , input temperature value range from -20℃-40℃.
When temperature symbol flash, indicating refrigeration system is running,
otherwise refrigeration stops working.
8.1.3 Start the operation
（1）Press button to start running
The door should be locked before rotor starts rotating.
It will start timing once the rotor starts running, the screen displays remaining run time.
（2）Inquiry and modify the operation parameters
Operation parameters can be modified after the centrifuge reaches setting speed.
Press select button , return to the preparation interface and displays setting parameters.
Press again the select button , once the parameter symbol is flashing, rotate parameter
button to modify parameters. Release button after 5 seconds, centrifuge will return to normal
operation mode and run process according to the new parameters.
After time parameter has been modified, the operation time is not cleared and will continue
to be accumulated.
（3）Alarm display
If anything wrong in processing, the centrifuge will brake to stop automatically, and display
the error code on the time area. You can find out the malfunction causes by checking the table
10-1, and make correct actions accordingly.

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8.1.4 End the operation
（1）The centrifuge will brake when it reaches the setting time or button is pressed.
When the rotor stops rotating, centrifuge will start beeping to alarm the operation was over.
（2）Open the door
The door can be released automatically when the operation was over.
After door closed, you are able to press the button to open it.
After ending the operation, the program will store the setting parameters of this operation, and
will recall these parameters when restart the program.
（3）Open the door and take out the rotor and sample.
8.2 RCF (Relative Centrifugal Force) operation
（1）Turn on the POWER switch
（2）Set a RCF value
CAUTION：
●Do not exceed the allowable maximum RCF value of the rotor and adapters.
Press the select button , choose speed unit ×g, the speed symbol will flash into
RCF parameter input status.
If no button is pressed after the speed parameter has flashed after 5 seconds, the
inputting mode will be shut down.
Rotate parameter button to input a RCF value, RCF increment is10×g.
（3）Set operating conditions.
The other operation, please refer to the section 8.1.
8.3 Pulse operation
This function is used to remove the residual samples adhered on the interior of the tubes.
（1）Turn on the POWER switch and load the rotor on the shaft, tighten the rotor door and make sure it is
in secured position, and then close the door.
Note：The button works only when the rotor stopped and the door locked.

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（2）The centrifuge gets into preparation mode and displays last running parameters.
（3）Press knob and hold, speed up to reach the maximum speed. While release knob during
acceleration, the centrifuge will start to decelerate and stop.
9.Maintenance
9.1 Cleaning
CAUTION：Do not follow the recommended instructions for cleaning or disinfecting
which may damage the centrifuge.
（1）Centrifuge
If the centrifuge is exposed to ultraviolet rays for a long time, the color of the doors may be
changed or the label may be came off. After using, cover the centrifuge with a piece of cloth
to protect it from direct exposure.
Once the centrifuge is heavily dirty, clean it with a cloth or sponge moistened with a neutral
detergent solution.
Sterilize the centrifuge by wiping with a cloth moistened with 70% ethanol solution.
（2）Rotor chamber
Once the rotor chamber is dirty, clean with cloth or sponge moistened with a neutral detergent
solution. Sterilize the centrifuge by wiping with a cloth moistened with 70% ethanol solution.
（3）Drive shaft
Recommend have a regular maintenance for drive shaft. You can wipe the drive shaft with soft
cloth, and then apply a thin coat of silicon grease to it.
（4）Door
Clean and sterilize the door using the same method as the step 1).
（5）Rotor
To prevent corrosion, remove the rotor from rotor chamber. If do not use for a lone term, then
CAUTION：Do not directly pour water, neutral detergent or disinfectant solution into
the rotor chamber. Otherwise fluids may leak into the drive units and cause corrosion or
deterioration to the bearings.

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detach the rotor door and upside the rotor down to dry the tube holes.
If a sample is leaked in the rotor, rinse the rotor with water. Apply a thin coat of silicon grease to
the rotor when it is completely dried.
The rotor should be regular maintenance, recommend to cleaning it each 3 months to ensure tube
and rotor holes keep clean, and then apply a thin coat of silicon grease.
（6）Drain (D3024R)
The centrifuge is equipped with drainage, drain off water when more water is in drain pipe.
9.2 Consumables
The table list below is the centrifuge consumables. It is recommended to replace consumables
according the table below.
No. Consumables Replacement conditions
1 Seal ring of door
Crack
2 Rubber block of temperature sensor
3 Seal ring of centrifuge chamber (3024R)
9.3 The replacement of seal rings
9.3.1 Instructions
There are three high-temperature rubber seal rings that equipped into rotor to achieve bio-safe. The seal
rings may fall off or aging after several autoclaving, need to be replaced or re-installed.
Figure9-1 Sealringsofrotor
9.3.2 Replacement methods
（1）Clean the seal ring slot with neutral detergent solution and make it dry.
（2）Evenly coated with glue (501) in the seal ring slot and keep the seal ring into slot, press evenly to
Seal ring 1 2 3 Seal ring 2 3