DORIC LISER & LED Series User manual

⋆LISERTM & LED/LD Light Source
User Manual
Version 1.0.0

Contents
1 Important Safety Information 3
1.1 General Safety Information ............................................. 3
1.2 Laser Safety Information .............................................. 3
1.3 Safety Labels ..................................................... 4
1.4 Activation Safety Features ............................................. 4
1.5 Emission Indicator .................................................. 5
2 Overview 6
2.1 Operating Principle ................................................. 6
2.2 Overview of the ⋆LISERTM & LED/LD Light Source ............................... 8
2.3 Overview of the Bandpass Filter for ⋆LISERTM ................................. 9
3 Operations Guide 10
3.1 Getting Started ................................................... 10
3.2 FC Connector Installation ............................................. 12
4 Doric Neuroscience Studio 13
4.1 Channel Configuration ............................................... 13
4.2 Control ans Settings ................................................. 21
4.3 Acquisition View ................................................... 23
5 Specifications 25
6 Support 27
6.1 Maintenance ..................................................... 27
6.2 Warranty ....................................................... 27
6.3 Disposition ...................................................... 27
6.4 Contact us ...................................................... 27
2

1
Important Safety Information
1.1 General Safety Information
The ⋆LISERTM & LED/LD Light Source is a new type of optical source which, in addition to the laser-pumped Ce:YAG
crystal fluorescence output, can also include standard LED or laser diode outputs. This type of hybrid light source
is not specifically considered by international safety committees such as the IEC1and the FDA2. Consequently, the
user should follow all safety procedures related to the worst case scenario, either in working or failure condition.
Considering the power level of the fluorescence output of the ⋆LISERTM & LED/LD Light Source, this means following
Class 3B laser product safety rules even though the output does not necessarily contain laser radiation, depending
on the exact model and output filter. The next section on laser safety information should thus be read and carefully
followed.
1.2 Laser Safety Information
If you are not familiar with laser light sources, ask for advice to qualified personnel BEFORE FIRST USE and READ
CAREFULLY the application note Important Laser Safety Information that can be found on the USB key. You can also
contact directly Doric Lenses by email ([email protected]) to obtain a copy of this application note.
DANGER!
The ⋆LISERTM & LED/LD Light Source is a Class 3B laser product.
Read the application note Important Laser Safety Information
BEFORE FIRST USE.
The ⋆LISERTM & LED/LD Light Source is a Class 3B laser product emitting visible light at sufficiently high power levels
to PERMANENTLY DAMAGE THE EYES. NEVER LOOK directly into the optical beam exiting from the output FC
connector or from any optical fiber connected to the output FC connector. NEVER LOOK directly at specular or
diffuse reflections of the output beam. It is important to WEAR LASER SAFETY GLASSES (goggles) certified for the
wavelength and power level of the light source. Also follow all safety procedures to protect anyone working in the
area. Even when wearing laser safety glasses, NEVER LOOK directly into the beam or any specular reflection of
the optical beam exiting from the ⋆LISERTM & LED/LD Light Source or from any optical fiber connected to its output
FC connector. The ⋆LISERTM & LED/LD Light Source is provided with a safety interlock connector on the rear panel.
When the interlock circuit is shorted and the power key is inserted, the driver is enabled (see Section 1.4). For a safe
use of the ⋆LISERTM & LED/LD Light Source, the safety interlock connector should be connected to the laser safety
interlock circuit of the laboratory. You should contact the laser safety officer (LSO) of your institution or company to
set a proper laser safety interlock circuit for your application and laboratory installation. The ⋆LISERTM & LED/LD Light
Source emits light spanning over a large bandwidth in the visible light spectrum. Since the output spectrum depends
on the exact model and optional output filter, the output power level and the according safety procedures are specific
to each application.
1International Electrotechnical Commission
2Food and Drug Administration
3

1.3 Safety Labels
The laser class labels are provided with the system and the laser aperture is clearly identified by laser warning label
and/or the text LASER APERTURE.
WARNING - LASER RADIATION
AVOID EXPOSURE TO BEAM
CLASS 3B LASER PRODUCT
WAVELENGTH: 400-700 nm
MAXIMUM OUTPUT POWER: <500 mW
IEC 60825-1 Edition 3.0 2014-05
(a) Laser Classification Label Example (b) Laser Warning Label (c) Laser Aperture Identification
Figure 1.1: Safety Labels
1.4 Activation Safety Features
The drivers for all Doric Lenses light sources come with a number of safety features. These are built into the driver
circuits, as shown in the block diagram (Fig. 1.2).
Figure 1.2: Safety feature block diagram
•The Micro-controller,Key Switch,Interlock Plug and Current Driver are connected in series. This means that
if any single safety feature is not properly in place, the light source cannot be activated.
•The Micro-controller is used to control the light source driver.
(a) Key Switch (b) Interlock Plug
Figure 1.3: Safety Feature Elements
•The Key Switch (Safety feature 1) (Fig. 1.3a), located on the left side of the driver, is required to activate any
light source. If removed, no data can be sent from the micro-controller to the Current Driver.
•The Interlock plug (Safety feature 2) (Fig. 1.3b) is used to integrate the driver into an Interlock Circuit.
–The Interlock Plug comes with a small wire short-circuiting it. This wire must be removed before integrating
it into an Interlock Circuit.
Chapter 1. Important Safety Information 4

–Connect the Interlock Circuit in series with the Interlock Plug so the circuit may function properly.
•The Current Driver sends current to any connected light source. If the Key is absent or the Interlock Plug has
an open circuit, it cannot receive signals from the micro-controller, preventing it from sending out current.
1.5 Emission Indicator
For light sources emitting invisible laser radiation, a dedicated LED indicator is ON when the driver is outputting an
electrical current. When the driver is outputting current, the light source will emit light from the aperture.
Chapter 1. Important Safety Information 5

2
Overview
2.1 Operating Principle
The Doric ⋆LISERTM light source is inspired by the “white” LED concept. LEDs (Light Emitting Diodes) are solid state
light sources emitting light by the direct conversion of an electric current passing through a semiconductor junction.
LEDs are now available at many central wavelengths and bandwidths. Properly speaking, no LEDs can emit over the
entire visible spectrum to produce white light. Many so-called “white” LEDs are in fact a combination of a blue LED
and a fluorescent crystal. A part of the blue LED light is absorbed by a Cerium doped Yttrium Aluminum Garnet crystal
(shortly: Ce:YAG crystal) which, after absorption of blue light, spontaneously re-emits over a significant part of the
visible spectrum, mostly green, yellow and red light. The combination of the green-yellow-red fluorescence and the
unabsorbed blue light from the pumping LED results in white light.
“White” LEDs are designed for energy efficient white lighting applications. For scientific applications involving optical
fiber coupling, high spectral brightness (W/mm2/sr/nm) sources are required. In order to increase the spectral bright-
ness, the “white” LED operating principle must be scaled in term of pump power. The power scaling first requires a
higher brightness pump source emitting blue light. In the Doric ⋆LISERTM light source, high power blue laser diodes
(LDs) emitting at 450 nm are used to provide a much smaller pumped volume of the Ce:YAG crystal. Such a direct
power scaling of the pumping source leads to thermal failures. Indeed, a high brightness pumping leads to a local
heating of the Ce:YAG crystal. When the crystal temperature locally reach about 400°C, a phenomenon called “tem-
perature quenching” leads to a significant drop of the fluorescent emission due to non radiative relaxation of blue-light
excited Ce3+ ions. The temperature increase ultimately leads to a catastrophic failure of the crystal. Heat generated
by high brightness pumping thus have to be managed properly using passive/active cooling and crystal geometry.
The ⋆LISERTM light source emits over a broad and continuous visible light spectrum. A typical fluorescence spectrum of
a Ce:YAG crystal is shown in Fig. 2.1. The source thus provides a speckle-free light beam at the output of multimode
optical fibers. Also, direct modulation of the emitted light intensity is easily achieved through the modulation of the
injection current of all pump laser diodes, and this, without any detrimental spiking like with diode-pumped solid-state
(DPSS) laser systems.
6

Figure 2.1: Output Power Spectral Density of a ⋆LISERTM Fiber Light Source using 200 µm, 0.53 NA Fiber
Chapter 2. Overview 7

2.2 Overview of the ⋆LISERTM & LED/LD Light Source
The Doric ⋆LISERTM & LED Light Source includes a LED light source combined with the ⋆LISERTM source (Fig. 2.2). The
light from both sources are mixed using an optical combiner. The ⋆LISERTM & LED/LD Light Source is considered as a
Class 3B laser product. It is critical to follow the safety instructions stated in this manual. The device has the following
elements.
Figure 2.2: ⋆LISERTM & LED/LD Fiber Light Source
•The Beam Aperture is where the light exits the light source. The aperture is composed of a fiber coupling
assembly that injects the emitted light into an optical fiber. The standard model uses an FC fiber connector. A
safety FC metal cap is attached to the optical head to block the output light beam in absence of optical fiber.
•The Fan Grids are found on the top, rear and side of the light source. They must be kept clear at all times to
avoid overheating the system.
•The Removable Filter Holder is used to insert an optical bandpass filter in the system, allowing the selection of
a narrow part of the broad Ce:YAG spectrum of emission. The filter holder can accept any filter up to a 25.4 mm
diameter and a maximum 5 mm thickness.
•The BNC LISER Input Port is used to command the ⋆LISERTM source.
•The BNC LED Input Port is used to command the LED/LD source.
•The BNC LISER Output Port is used to monitor the ⋆LISERTM source.
•The BNC LED Output Port is used to monitor the LED/LD source.
•The Power On/Off switch turns on/off the driver.
•The Key Switch must be in place to enable light emission. Note that, despite its similar form factor, the power
key is not a standard micro SD card such as those used in some digital cameras. Do not attach the Key to a key
fob or similar holder; this may prevent proper insertion of the Key Switch.
•The 12 V port connects the ⋆LISERTM to its 12 VDC power supply.
•The USB port allows the user to connect the ⋆LISERTM to a computer using a USB-A/USB-B cable. This allows
the driver to be controlled using Doric Neuroscience Studio.
•The Interlock connector plug allows the user to connect the ⋆LISERTM to a safety interlock system. It is recom-
mended to connect the interlock plug to a laboratory interlock system (See chapter 1).
Chapter 2. Overview 8

2.3 Overview of the Bandpass Filter for ⋆LISERTM
Considering the very broad spectrum of the ⋆LISERTM fluorescence, it is often required to select a specific part of
the output spectrum using an optical bandpass filter. The light source contains a Removable Filter Holder that can
accept filters with an outside diameter of 25.4 mm and maximum thickness of 5 mm. The filter is placed between the
⋆LISERTM light source and the combiner, and is held in place magnetically. A wide variety of filters are available in our
Website.
Figure 2.3: Bandpass Filter for the ⋆LISERTM in its holder
Chapter 2. Overview 9

3
Operations Guide
3.1 Getting Started
The procedure below should be followed carefully. There are several safety measures to take into account to ensure
safe and proper use of the ⋆LISERTM & LED/LD Light Source.
1. Connect the Interlock plug to the driver. The system CANNOT be operational if the safety interlock circuit is
open.
•When unpacking, a temporarily shorted interlock plug is already secured in place. It is highly recommended
to remove the shorting electrical wire and connect the interlock plug to a proper interlock circuit of the
laboratory.
•See the Important Safety Information section (Chapter 1) for more information.
WARNING!
Be aware that a shorted interlock plug DISABLES this safety feature AT YOUR
OWN RISKS. A proper safety interlock circuit is highly recommended.
2. Ensure that the ON/OFF switch of the driver is set to OFF.
3. Connect the ⋆LISERTM & LED/LD Light Source to the power outlet with the included 12 V AC-DC adapter.
WARNING!
DO NOT OPEN the enclosure of the driver. Electrical hazards may result.
The driver does not contain any user-serviceable components.
4. The optical head is sensitive to electrostatic discharges, use proper grounding techniques.
5. Unscrew the metal safety cap from the Beam aperture.
6. Connect the Optical fiber patch-cord to the ⋆LISERTM & LED/LD Light Source (see the section 3.2).
7. Ensure that a proper laser beam block is ready at the output of the optical fiber.
DANGER!
The light beam exiting the ⋆LISERTM & LED/LD Light Source or any connected optical
fiber should be confined properly BEFORE turning ON the device.
Use a proper beam block. Read the application note Important Laser Safety
Information BEFORE FIRST USE.
8. Ensure that all laser safety procedures are followed.
10

9. Insert the power key into its receptacle.
10. Set the power switch to ON.
11. Connect the USB-A/USB-B cable between the ⋆LISERTM & LED/LD Light Source and the computer.
12. Install the Doric Neuroscience Studio on the computer. Double-click on the DoricNeuroscienceStudioSetup_vX.X.exe
file located on the Doric USB memory stick supplied with the ⋆LISERTM & LED/LD Light Source and follow the
on-screen instructions (help is also available in the Doric Neuroscience Studio User Manual, which can be
downloaded on our Website, section SUPPORT).
13. If needed, connect an external device to the ⋆LISERTM & LED/LD Light Source by using one of the input BNC
port. In this configuration, the driver will wait the signal from another device, consequently the light source will
be triggered. To follow an external device, select the external TTL or the external analog mode for the desired
channel (see section 4.1.2).
14. If needed, connect a data acquisition system or an external device to the ⋆LISERTM & LED/LD Light Source by using
the output BNC port of the desired channel. A data acquisition system enables the viewing and the recording
of the signal generated by an external device connected to the light source. The output BNC can also be used
to connect an external device that will be triggered by the light source.
LISER™/LD 450 Light Source
Fiber optic rotary joint
(FRJ_1x1_FC)
secure to a holder
(Holder_FRJ_small)
Branching ber optic
patch cord (BFP)
Two ferrule cannula
(TFC)
Doric Neuroscience Studio
FRJ_1x1_FC-FC
Figure 3.1: System using a ⋆LISERTM & LED/LD Light Source
Chapter 3. Operations Guide 11

3.2 FC Connector Installation
1. Clean the optical fiber connector before insertion. Use isopropanol and a lint-free wipe.
2. With an FC connector, the connector key must be oriented to enter within the receptacle slot to ensure proper
connection (Fig. 3.2).
Figure 3.2: FC connector, Fiber Installation
To reduce the risk of eye injury, it is sound practice to
NOT CONNECT/DISCONNECT OPTICAL FIBERS
when the light source is turned on
Chapter 3. Operations Guide 12

4
Doric Neuroscience Studio
Doric Light Sources can be controlled by the Doric Neuroscience Studio. These include LED Modules, Laser Diode
Modules and ⋆LISERTM Light Source1. The interface is separated into two main sections, Control & settings and the
Acquisition View. Each light source driver has a number of Channels, each one controlling a light source of its given
type. These channels, accessible using the Add Channel button, will be the first detailed.
4.1 Channel Configuration
4.1.1 Channel Configuration Window Overview
Figure 4.1: Channels Configuration Main Interface
The Channels configuration window is used to configure each channel. The window can be accessed by using either
the Add Channel or Edit buttons. This window is separated into multiple sections shown in Figure 4.1 that are defined
below.
1The ⋆LISERTM Light Source are also known in older models as Ce:YAG Fiber Light Source.
13

1. The Channel Types are displayed on the left side of the window. These include the ⋆LISERTM light sources, the
LED light sources and the Laser Diode light sources.
2. The Channel Options section allows you to define the Light Source Option, the Current options and the Trig-
gering Options. The different fields of this section are explained in more detail in section 4.1.2.
3. The Sequence Options defines the parameters of each pulse sequence for the channel. These parameters are
different for each Channel Mode. The different fields for the different Channel Mode are explained in more
detail in section 4.1.3.
4. The Sequence Preview section shows a visualization of the output sequence that will be generated by the
current configuration.
5. The Add button will save the current channel configuration and enables a new channel to be configured. The
Close button will close the window without saving the current channel configuration.
4.1.2 Channel Options Section
Figure 4.2: Channel Options of the Channel Configuration Window
The Channel Option section (Fig. 4.2) is separated in 3 sub-sections, the LightSource Options section that defines
the channel and its mode, the Current Options and the Trigger Options section that control the trigger method of
the selected channel.
LightSource Options
1. The Channel field identifies which of the available channels is currently being modified. The Light Source can
be changed by selecting a new one from the drop-down list.
2. The Mode field identifies the mode used to generate the light. Five modes are available, Continuous Wave
(fix current), External TTL (external digital command), External Analog (external analog command), Square Se-
quence(s) (internal digital command), and Complex Sequences(s) mode (internal analog command). Each mode
enables different options of the Sequence Option section that are explained in more detail in section 4.1.3.
Chapter 4. Doric Neuroscience Studio 14

Current Options
3. The Current Options includes the slider used to control the current sent to the light source.
•When using some LED module, the Overdrive checkbox will appear. When selected, this allows the system
to exceed the normal safe current limit of the light source. THIS SHOULD ONLY BE USED WITH PULSED
SIGNALS, AS IT CAN OTHERWISE DAMAGE THE LIGHT SOURCE.
•When using a CLED module, the Low-Power checkbox will appear. When selected, this allows reduced-
power signaling for the same voltage. This mode is only available for CLED modules. This allows low-power
signals to be more stable in time. The maximal current is reduced to one tenth of light source’s normal
maximal current. If the BNC Output is used to monitor the LED power, its output voltage is proportional
to the current passing through the light source, and not the voltage sent to it. For example, a driver with a
normal maximum current of 2000 mA for a 5 V signal (400 mA/V) will have a maximum current of 200 mA
for a 5 V signal (40 mA/V) in low power mode. The BNC output of the driver will still relate LED current
with a 400 mA/V conversion factor.
Trigger Options
4. The Type defines the type of trigger that is used to start/stop a sequence. The Triggered type can starts and
stops a sequence at a rising edge while the Gated type can starts the sequence at a rising edge and stops it at a
falling edge. A more refined interaction of the trigger with the defined sequence can be set up using the Mode
field. Not all Trigger Type are available for each combination of Trigger Mode and Repeatability. The different
combinations are shown in Figure 4.7.
5. The Mode field defines how the trigger activates a sequence. Each mode are not compatible with each combi-
nation of trigger type and repeatability. Figure 4.7 shows the different available combinations for the different
Trigger Modes. Four Modes are available and are the following:
•Uninterrupted: This mode activates the channel sequence when an input greater than 3.3 V is detected
by the BNC input. Following input pulses will be ignored while the sequence is running (Fig. 4.3). When
the Repeatable sequence checkbox is checked, the sequence will restart with the arrival of the first input
pulse after the sequence has finished (Fig. 4.3b). This mode is available for Triggered pulse only.
•Pause: This mode activates the channel sequence when a rising edge greater than 3.3 V is detected on
the BNC input (Fig. 4.4). Following input pulses (when Triggered, Fig. 4.4a) or falling edge (when Gated,
Fig. 4.4c) will pause the sequence and the sequence will continue when the next rising edge is received.
When the Repeatable sequence checkbox is checked, the sequence will restart with the arrival of the first
input pulse after the sequence has finished (Figs. 4.4b and 4.4d).
•Continue: This mode activates the channel sequence when a rising edge greater than 3.3 V is detected on
the BNC input (Fig. 4.5). The following input pulse (when Triggered, Fig. 4.5a) or a falling edge (when Gated,
Fig. 4.5c) will turn off the output, but the sequence will continue. The output will be turned back on at the
reception of the following rising edge. Triggers only affect the output voltage value. When the Repeatable
sequence checkbox is checked, the sequence will restart with the arrival of the first input pulse after the
sequence has finished (Figs. 4.5b and 4.5d).
•Restart: This mode activates the channel sequence when a rising edge higher than 3.3 V is detected on the
BNC input. The following input pulse (when Triggered, Fig. 4.6a) or falling edge (when Gated, Fig. 4.6b) will
stop the sequence and the sequence will restart from the beginning when the next rising edge is received.
When the sequence is completed, it will restart with the next input pulse.
Chapter 4. Doric Neuroscience Studio 15

Output Triggers
(a) Triggered Non-Repeatable Sequence
...
Output Triggers
(b) Triggered Repeatable Sequence
Figure 4.3: Uninterrupted Sequence Mode
Output Triggers
(a) Triggered Non-Repeatable Sequence
...
Output Triggers
(b) Triggered Repeatable Sequence
Output Triggers
(c) Gated Non-Repeatable Sequence
...
Output Triggers
(d) Gated Repeatable Sequence
Figure 4.4: Pause Sequence Mode
Output Triggers
(a) Triggered Non-Repeatable Sequence
...
Output Triggers
(b) Triggered Repeatable Sequence
Output Triggers
(c) Gated Non-Repeatable Sequence
...
Output Triggers
(d) Gated Repeatable Sequence
Figure 4.5: Continue Sequence Mode
...
Output Triggers
(a) Triggered Repeatable Sequence
...
Output Triggers
(b) Triggered Gated Sequence
Figure 4.6: Restart Sequence Mode
Chapter 4. Doric Neuroscience Studio 16

6. The Repeatable sequence checkbox, when selected, allows a sequence to be repeated. Not all modes and
trigger types can be repeated. Please refer to the Figure 4.7 to know the repeatable sequence combinations.
7. The TTL Output checkbox, when selected, allows the output BNC channel to be used as a TTL generator. The
monitoring signal will provide a TTL signal instead of an analog voltage output proportional to the LED current.
The output will send out a 5 V signal whenever the input current is >0 mA. This can be used even if a light
source is not connected.
8. The Sequence Visualisation shows a graphical representation of the behavior of the selected Trigger Option
Type, Mode and Repeatability.
Triggered Gated
Non-repeatable
sequence
Repeatable
sequence
Non-repeatable
sequence
Repeatable
sequence
Uninterrupted
Pause
Con�nue
Restart
Figure 4.7: Trigger options possibilities
4.1.3 Sequence(s) Options Section
Continuous Wave
The Continuous Wave mode is used to set the Light Source to a chosen intensity without variations during experi-
ments.
External TTL
The External TTL mode is used to drive the Light Source to a chosen intensity when the External TTL signal is high.
When the External TTL signal is low, the Light Source is turned OFF.
External analog
The External Analog mode is used to drive the Light Source in function of the analog voltage used as input. The input
voltage may varies between 0 V and 5 V and the intensity will follow the variations between 0 mA and the maximum
current.
Chapter 4. Doric Neuroscience Studio 17

Square Sequence(s)
(a) Square Sequence(s) Mode Interface
(b) Exemple of Error
Figure 4.8: Sequence Options of the Square Sequence(s) Mode.
The Square Sequence(s) mode allows the creation of a square TTL pulse sequence. The Sequence(s) Options of this
mode are shown in Figure 4.8a and are explained below.
1. The Starting Delay defines the time between the activation of the pulse sequence and the beginning of the first
light illumination.
2. The Frequency sets the frequency (in Hz), which is the number of pulses per second. The frequency can also be
changed to the Period. For example, a light illumination at 10 Hz (frequency) will output one pulse every 100
ms (period), whereas a light illumination at 0.5 Hz (frequency) will output one pulse every 2 seconds (period).
3. The Time ON defines the length of a single pulse. This time can also be converted to a Duty Cycle, which
represents the % of the period the pulse duration corresponds to.
4. The Smoothing check box allows to change the pulse slope in square pulse sequences. The Edit Edges button
opens the Smoothing Edge(s) window. An overview of the window opened by Edit Edges will be done in the
next subsection.
5. The Pulse(s) per sequence set the number of pulses per sequence. If it is set to 0, the number of pulses will be
infinite.
6. The Number of sequence(s) sets the number of times that the sequence will be repeated.
7. The Delay between sequences sets the delay between each sequence.
8. The Total Duration shows the total expected duration of the pulse sequence. Should the duration be infinite,
the box will display ∞. If there is an error in parameter selection, this box will turn red and display what is the
error (see Figure 4.8b).
Chapter 4. Doric Neuroscience Studio 18

Smoothing Edge(s)
The Smoothing Edge(s) window (Fig. 4.9) allows to change the pulse slopes of the square pulse sequences.
(a) Overview of the Smoothing Edge(s) window
(b) Exemple of smoothing edges (10ms for rise and fall time)
Figure 4.9: Smoothing Edge(s) window
1. The Rise Time box is used to define the duration to rise from 0 mA to the pulse maximum value.
2. The Plateau Time box is used to define the duration the pulse at its maximum value.
3. The Fall Time box is used to define the duration to descend from the pulse maximum value to 0 mA.
4. The Pulse Graph displays the pulse shape.
5. The Active Time box displays the total duration of the pulse. While the Smoothing option is active, the Time
ON is fixed at this value.
6. The OK button save the changes of the shape of the pulses. The Cancel button discard the changes. Both
buttons close the window.
Chapter 4. Doric Neuroscience Studio 19

Complex Sequence(s)
If needed, it is possible to define a complex sequence to trigger the light source in the Complex Sequence(s) Options
(Fig. 4.10).
Figure 4.10: Complex Sequences Window
1. The Starting Delay sets the delay (in hh:mm:ss:zzz format) before the first light illumination.
2. The Current sets the maximum current (in mA) for the given sequence.
3. The Nb. Seq. sets the number of times that the sequence will be repeated, with a minimum of 1.
4. The Delay between sequences sets the delay (in hh:mm:ss:zzz format) between each sequence if Nb.Seq. is
greater than 1.
5. The Pulses per Seq. sets the number of pulses per sequence, with a minimum of 1.
6. The Frequency/Period sets the frequency (in Hz) or period (in ms) for the pulse sequence. These two values are
linked, and when one is changed the other will adjust automatically. For example, a signal at 10 Hz (frequency)
will output one pulse every 100 ms (period), whereas a pulse sequence at 0.5 Hz (frequency) will output one
pulse every 2000 ms (period).
7. The Time ON/Duty Cycle sets the time (in ms) or the duty cycle (in %) for each pulse. These two values are linked,
and when one is changed the other will adjust automatically. The Time ON must be lower than Period+0.005 ms,
while the Duty cycle must be below 100 %.
8. The Types of pulses sets the pulse type. Pulses can be Square, triangular (Triangle), Ramp up,Ramp down or
Delay. The Delay pulse type is used to create a delay between different sequence.
9. The Sequence controls allow the addition (+) or removal (-) of sequences to the spreadsheet.
10. The Total Duration displays the total time of the experiment. The different values can be Inf for infinite, a valid
time value or Err if the Time ON value is greater than the Period.
Chapter 4. Doric Neuroscience Studio 20