DORIC LISER & LED Series User manual

⋆LISERTM & LED/LD Light Source
User Manual
Version 1.0.0

Contents
1 Important Safety Information 3
1.1 General Safety Information ............................................. 3
1.2 Laser Safety Information .............................................. 3
1.3 Safety Labels ..................................................... 4
1.4 Activation Safety Features ............................................. 4
1.5 Emission Indicator .................................................. 5
2 Overview 6
2.1 Operating Principle ................................................. 6
2.2 Overview of the ⋆LISERTM & LED/LD Light Source ............................... 8
2.3 Overview of the Bandpass Filter for ⋆LISERTM ................................. 9
3 Operations Guide 10
3.1 Getting Started ................................................... 10
3.2 FC Connector Installation ............................................. 12
4 Doric Neuroscience Studio 13
4.1 Channel Configuration ............................................... 13
4.2 Control ans Settings ................................................. 21
4.3 Acquisition View ................................................... 23
5 Specifications 25
6 Support 27
6.1 Maintenance ..................................................... 27
6.2 Warranty ....................................................... 27
6.3 Disposition ...................................................... 27
6.4 Contact us ...................................................... 27
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1
Important Safety Information
1.1 General Safety Information
The ⋆LISERTM & LED/LD Light Source is a new type of optical source which, in addition to the laser-pumped Ce:YAG
crystal fluorescence output, can also include standard LED or laser diode outputs. This type of hybrid light source
is not specifically considered by international safety committees such as the IEC1and the FDA2. Consequently, the
user should follow all safety procedures related to the worst case scenario, either in working or failure condition.
Considering the power level of the fluorescence output of the ⋆LISERTM & LED/LD Light Source, this means following
Class 3B laser product safety rules even though the output does not necessarily contain laser radiation, depending
on the exact model and output filter. The next section on laser safety information should thus be read and carefully
followed.
1.2 Laser Safety Information
If you are not familiar with laser light sources, ask for advice to qualified personnel BEFORE FIRST USE and READ
CAREFULLY the application note Important Laser Safety Information that can be found on the USB key. You can also
contact directly Doric Lenses by email ([email protected]) to obtain a copy of this application note.
DANGER!
The ⋆LISERTM & LED/LD Light Source is a Class 3B laser product.
Read the application note Important Laser Safety Information
BEFORE FIRST USE.
The ⋆LISERTM & LED/LD Light Source is a Class 3B laser product emitting visible light at sufficiently high power levels
to PERMANENTLY DAMAGE THE EYES. NEVER LOOK directly into the optical beam exiting from the output FC
connector or from any optical fiber connected to the output FC connector. NEVER LOOK directly at specular or
diffuse reflections of the output beam. It is important to WEAR LASER SAFETY GLASSES (goggles) certified for the
wavelength and power level of the light source. Also follow all safety procedures to protect anyone working in the
area. Even when wearing laser safety glasses, NEVER LOOK directly into the beam or any specular reflection of
the optical beam exiting from the ⋆LISERTM & LED/LD Light Source or from any optical fiber connected to its output
FC connector. The ⋆LISERTM & LED/LD Light Source is provided with a safety interlock connector on the rear panel.
When the interlock circuit is shorted and the power key is inserted, the driver is enabled (see Section 1.4). For a safe
use of the ⋆LISERTM & LED/LD Light Source, the safety interlock connector should be connected to the laser safety
interlock circuit of the laboratory. You should contact the laser safety officer (LSO) of your institution or company to
set a proper laser safety interlock circuit for your application and laboratory installation. The ⋆LISERTM & LED/LD Light
Source emits light spanning over a large bandwidth in the visible light spectrum. Since the output spectrum depends
on the exact model and optional output filter, the output power level and the according safety procedures are specific
to each application.
1International Electrotechnical Commission
2Food and Drug Administration
3

1.3 Safety Labels
The laser class labels are provided with the system and the laser aperture is clearly identified by laser warning label
and/or the text LASER APERTURE.
WARNING - LASER RADIATION
AVOID EXPOSURE TO BEAM
CLASS 3B LASER PRODUCT
WAVELENGTH: 400-700 nm
MAXIMUM OUTPUT POWER: <500 mW
IEC 60825-1 Edition 3.0 2014-05
(a) Laser Classification Label Example (b) Laser Warning Label (c) Laser Aperture Identification
Figure 1.1: Safety Labels
1.4 Activation Safety Features
The drivers for all Doric Lenses light sources come with a number of safety features. These are built into the driver
circuits, as shown in the block diagram (Fig. 1.2).
Figure 1.2: Safety feature block diagram
•The Micro-controller,Key Switch,Interlock Plug and Current Driver are connected in series. This means that
if any single safety feature is not properly in place, the light source cannot be activated.
•The Micro-controller is used to control the light source driver.
(a) Key Switch (b) Interlock Plug
Figure 1.3: Safety Feature Elements
•The Key Switch (Safety feature 1) (Fig. 1.3a), located on the left side of the driver, is required to activate any
light source. If removed, no data can be sent from the micro-controller to the Current Driver.
•The Interlock plug (Safety feature 2) (Fig. 1.3b) is used to integrate the driver into an Interlock Circuit.
–The Interlock Plug comes with a small wire short-circuiting it. This wire must be removed before integrating
it into an Interlock Circuit.
Chapter 1. Important Safety Information 4

–Connect the Interlock Circuit in series with the Interlock Plug so the circuit may function properly.
•The Current Driver sends current to any connected light source. If the Key is absent or the Interlock Plug has
an open circuit, it cannot receive signals from the micro-controller, preventing it from sending out current.
1.5 Emission Indicator
For light sources emitting invisible laser radiation, a dedicated LED indicator is ON when the driver is outputting an
electrical current. When the driver is outputting current, the light source will emit light from the aperture.
Chapter 1. Important Safety Information 5

2
Overview
2.1 Operating Principle
The Doric ⋆LISERTM light source is inspired by the “white” LED concept. LEDs (Light Emitting Diodes) are solid state
light sources emitting light by the direct conversion of an electric current passing through a semiconductor junction.
LEDs are now available at many central wavelengths and bandwidths. Properly speaking, no LEDs can emit over the
entire visible spectrum to produce white light. Many so-called “white” LEDs are in fact a combination of a blue LED
and a fluorescent crystal. A part of the blue LED light is absorbed by a Cerium doped Yttrium Aluminum Garnet crystal
(shortly: Ce:YAG crystal) which, after absorption of blue light, spontaneously re-emits over a significant part of the
visible spectrum, mostly green, yellow and red light. The combination of the green-yellow-red fluorescence and the
unabsorbed blue light from the pumping LED results in white light.
“White” LEDs are designed for energy efficient white lighting applications. For scientific applications involving optical
fiber coupling, high spectral brightness (W/mm2/sr/nm) sources are required. In order to increase the spectral bright-
ness, the “white” LED operating principle must be scaled in term of pump power. The power scaling first requires a
higher brightness pump source emitting blue light. In the Doric ⋆LISERTM light source, high power blue laser diodes
(LDs) emitting at 450 nm are used to provide a much smaller pumped volume of the Ce:YAG crystal. Such a direct
power scaling of the pumping source leads to thermal failures. Indeed, a high brightness pumping leads to a local
heating of the Ce:YAG crystal. When the crystal temperature locally reach about 400°C, a phenomenon called “tem-
perature quenching” leads to a significant drop of the fluorescent emission due to non radiative relaxation of blue-light
excited Ce3+ ions. The temperature increase ultimately leads to a catastrophic failure of the crystal. Heat generated
by high brightness pumping thus have to be managed properly using passive/active cooling and crystal geometry.
The ⋆LISERTM light source emits over a broad and continuous visible light spectrum. A typical fluorescence spectrum of
a Ce:YAG crystal is shown in Fig. 2.1. The source thus provides a speckle-free light beam at the output of multimode
optical fibers. Also, direct modulation of the emitted light intensity is easily achieved through the modulation of the
injection current of all pump laser diodes, and this, without any detrimental spiking like with diode-pumped solid-state
(DPSS) laser systems.
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Figure 2.1: Output Power Spectral Density of a ⋆LISERTM Fiber Light Source using 200 µm, 0.53 NA Fiber
Chapter 2. Overview 7

2.2 Overview of the ⋆LISERTM & LED/LD Light Source
The Doric ⋆LISERTM & LED Light Source includes a LED light source combined with the ⋆LISERTM source (Fig. 2.2). The
light from both sources are mixed using an optical combiner. The ⋆LISERTM & LED/LD Light Source is considered as a
Class 3B laser product. It is critical to follow the safety instructions stated in this manual. The device has the following
elements.
Figure 2.2: ⋆LISERTM & LED/LD Fiber Light Source
•The Beam Aperture is where the light exits the light source. The aperture is composed of a fiber coupling
assembly that injects the emitted light into an optical fiber. The standard model uses an FC fiber connector. A
safety FC metal cap is attached to the optical head to block the output light beam in absence of optical fiber.
•The Fan Grids are found on the top, rear and side of the light source. They must be kept clear at all times to
avoid overheating the system.
•The Removable Filter Holder is used to insert an optical bandpass filter in the system, allowing the selection of
a narrow part of the broad Ce:YAG spectrum of emission. The filter holder can accept any filter up to a 25.4 mm
diameter and a maximum 5 mm thickness.
•The BNC LISER Input Port is used to command the ⋆LISERTM source.
•The BNC LED Input Port is used to command the LED/LD source.
•The BNC LISER Output Port is used to monitor the ⋆LISERTM source.
•The BNC LED Output Port is used to monitor the LED/LD source.
•The Power On/Off switch turns on/off the driver.
•The Key Switch must be in place to enable light emission. Note that, despite its similar form factor, the power
key is not a standard micro SD card such as those used in some digital cameras. Do not attach the Key to a key
fob or similar holder; this may prevent proper insertion of the Key Switch.
•The 12 V port connects the ⋆LISERTM to its 12 VDC power supply.
•The USB port allows the user to connect the ⋆LISERTM to a computer using a USB-A/USB-B cable. This allows
the driver to be controlled using Doric Neuroscience Studio.
•The Interlock connector plug allows the user to connect the ⋆LISERTM to a safety interlock system. It is recom-
mended to connect the interlock plug to a laboratory interlock system (See chapter 1).
Chapter 2. Overview 8

2.3 Overview of the Bandpass Filter for ⋆LISERTM
Considering the very broad spectrum of the ⋆LISERTM fluorescence, it is often required to select a specific part of
the output spectrum using an optical bandpass filter. The light source contains a Removable Filter Holder that can
accept filters with an outside diameter of 25.4 mm and maximum thickness of 5 mm. The filter is placed between the
⋆LISERTM light source and the combiner, and is held in place magnetically. A wide variety of filters are available in our
Website.
Figure 2.3: Bandpass Filter for the ⋆LISERTM in its holder
Chapter 2. Overview 9

3
Operations Guide
3.1 Getting Started
The procedure below should be followed carefully. There are several safety measures to take into account to ensure
safe and proper use of the ⋆LISERTM & LED/LD Light Source.
1. Connect the Interlock plug to the driver. The system CANNOT be operational if the safety interlock circuit is
open.
•When unpacking, a temporarily shorted interlock plug is already secured in place. It is highly recommended
to remove the shorting electrical wire and connect the interlock plug to a proper interlock circuit of the
laboratory.
•See the Important Safety Information section (Chapter 1) for more information.
WARNING!
Be aware that a shorted interlock plug DISABLES this safety feature AT YOUR
OWN RISKS. A proper safety interlock circuit is highly recommended.
2. Ensure that the ON/OFF switch of the driver is set to OFF.
3. Connect the ⋆LISERTM & LED/LD Light Source to the power outlet with the included 12 V AC-DC adapter.
WARNING!
DO NOT OPEN the enclosure of the driver. Electrical hazards may result.
The driver does not contain any user-serviceable components.
4. The optical head is sensitive to electrostatic discharges, use proper grounding techniques.
5. Unscrew the metal safety cap from the Beam aperture.
6. Connect the Optical fiber patch-cord to the ⋆LISERTM & LED/LD Light Source (see the section 3.2).
7. Ensure that a proper laser beam block is ready at the output of the optical fiber.
DANGER!
The light beam exiting the ⋆LISERTM & LED/LD Light Source or any connected optical
fiber should be confined properly BEFORE turning ON the device.
Use a proper beam block. Read the application note Important Laser Safety
Information BEFORE FIRST USE.
8. Ensure that all laser safety procedures are followed.
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