DSI 600-2100-001 User manual

Life Science SuiteTM
Plethysmograph Chambers
Manual
Model: 600-2100-001, 600-2200-001, 600-2400-001, 600-2500-001, & 600-2600-001
Manual: MU00249-001
Revision 57
Data Sciences International
119 14th Street NW, Suite 100
St. Paul, MN 55112
P+[phone] F+1(651) 481-7470
http://www.datasci.com

Data Sciences International
119 14th Street NW, Suite 100
St. Paul, MN 55112
http://www.datasci.com
Copyright©2012 by Data Sciences International. All rights reserved. No part of this manual may be reproduced,
translated, transcribed, or transmitted in any form or by any means manual, electronic, electromagnetic, chemical, or
optical without the written permission of Data Sciences International.

Plethysmograph Chamber Manual Contents •i
Contents
Overview 1
Introduction ...............................................................................................................................1
Components...............................................................................................................................2
Rat Head-Out Plethysmograph Chamber ....................................................................2
Mouse Head-Out Plethysmograph Chamber...............................................................5
Rat Unrestrained Whole Body Plethysmograph Chamber ..........................................8
Mouse Unrestrained Whole Body Plethysmograph Chamber...................................12
Accessories..............................................................................................................................18
Operation 22
Setup and Operation ................................................................................................................22
DSI Head-Out Chambers Animal Loading Tips........................................................22
DSI Whole Body Chamber Animal Loading Tips.....................................................23
Maintenance and Cleaning.......................................................................................................23
Appendix A 24
Specifications...........................................................................................................................24
Troubleshooting.......................................................................................................................25
Insufficient Screen Resistance...................................................................................25
Compromised Neck Seal...........................................................................................26
Incorrect Gain Settings..............................................................................................26
Turbulent Air Flow....................................................................................................26
Animal Acclimation ..................................................................................................27
Product Issue Report 28
Product Issue Report Form......................................................................................................28
Feature Request 29
Feature Request Form..............................................................................................................29

Plethysmograph Chamber Manual Contents •1
Overview
Introduction
The DSI plethysmograph chambers are designed and built for optimal performance
along with the Ponemah Physiological Platform and DSI telemetry systems. Product
offerings in this document include the Rat Head-Out Plethysmograph Chamber, the
Mouse Head-Out Plethysmograph Chamber, the Guinea Pig Unrestrained Whole
Body Plethysmograph Chamber, the Rat Unrestrained Whole Body Plethysmograph
Chamber, and the Mouse Unrestrained Whole Body Plethysmograph Chamber.

2 •Contents Plethysmograph Chamber Manual
Components
Rat Head-Out Plethysmograph Chamber
Rat Head-Out Plethysmograph Chamber (600-2100-001) pictured on a telemetry RPC1 receiver plate

Plethysmograph Chamber Manual Contents •3
Rat Head-Out Plethysmograph Chamber components:
1. Rat Head-Out Plethysmography Chamber Accessory (Front) (600-
2110-001)
2. Squeeze plate mechanism (600-2112-001)
3. Rat Head-Out Plethysmography Chamber Accessory (Rear) (600-
2111-001)

4 •Contents Plethysmograph Chamber Manual
The Rat Head-Out Plethysmograph Chamber (600-2100-001) is packaged with the
following items:
1. Taper Plug 5/16 to ½ OD
2. 1/8-27 NPT to 1/8 barb
3. 1/8-27 NPT to 1/8-27 NPT
4. 1/8-27 NPT Plug
5. 1/8-27 NPT to ¼-28 UNF
6. Tygon tubing 1/8 in. ID (24 in. long)
7. Extra Pneumotachograph Screens (3)
8. Neck seals (2) 0.80 diameter hole
9. Neck seals (2) 0.95 diameter hole
10. Taper Plug 1 1/32 to 1 17/64 OD
11. Knurled thumb screw
12. Neck seals (2) silicon 0.75 hole
13. Neck seals (2) silicon 0.875 hole
14. Input block

Plethysmograph Chamber Manual Contents •5
Mouse Head-Out Plethysmograph Chamber
Mouse Head-Out Plethysmograph Chamber (600-2200-001)
Mouse Head-Out Plethysmograph Chamber (600-2200-001) components:
1. Body Chamber

6 •Contents Plethysmograph Chamber Manual
2. Head Mount Plate
3. Head Plate
4. Screw Pan HD 10-32x7/8
5. Knurled Thumb Nut
6. Female Luer Adaptor
7. Male Luer Plug
8. Male Luer Intergral Lock Ring
9. 1/8 dia x 3/16 screw
10. Stack Airway
11. Screen Retainer Plate
12. Pneumotachograph Screen
13. Black Silicone O-Ring
14. Screw Pan HD 4-40 x 7/16
15. Push Rod Guide
16. Push Rod Retainer Plate Seal
17. Red Silicone O-Ring Seal
18. Black Silicone O-Ring Seal
19. Screw Pan HD 4.40 x 1/4
20. Block Transducer Connection
22. Push Rod Assembly
The Mouse Head-Out Plethysmograph Chamber (600-2200-001) is packaged with
the following items:

Plethysmograph Chamber Manual Contents •7
1. 3 extra Pneumotachograph Screens
2. 2 Fittings, Luer male to 1/8 barb
3. Taper plug 7/16 to 5/8 OD
4. Taper plug 3/16 to 11/32 OD
5. 3 Latex neck seals
6. Tygon tubing 1/8 in. ID (24 in. long)
7. Head orifice reduction rings

8 •Contents Plethysmograph Chamber Manual
Rat and Guinea Pig Unrestrained Whole Body
Plethysmograph Chamber

Plethysmograph Chamber Manual Contents •9
Rat and Guinea Pig Unrestrained Whole Body Plethysmograph Chamber (600-2400-001, 600-2600-001) components:
1. Reference Chamber, Top
2. Reference Chamber, Cylinder
3. Reference Chamber, Bottom
4. Reference Chamber, Tube
5. Reference Chamber, Screen Mount
6. Main Chamber, Cylinder
7. Main Chamber, Top

10 •Contents Plethysmograph Chamber Manual
8. O-Ring Silicone Red
9. Latch
10. Body Box Screen Plate
11. Reference Chamber, Screen Plate
12. O-Ring Silicone Red
13. Plate, Air Path
14. Aerosolization port
15. Fitting, Luer Female to 1/8-27NPT
16. Screw Pan HD 4-40x1/4
17. Screw Pan HD 6-32x5/16
18. Screw Pan HD 4/40x7/8
19. Water Bottle Adapter
22. Latch Bracket
23. Screen Floor Assembly
24. Dowel Pin, SS .0626x0.5
25. Pneumotachograph Screen: SS 200x600
26. O-Ring, 1.00 OD
27. Rubber Silicone Foot .50SQ
28. O-Ring Silicone Red .50OD
29. Dowel Pin .125x .5 SS

Plethysmograph Chamber Manual Contents •11
The Rat and Guinea Pig Unrestrained Whole Body Plethysmograph Chambers are packaged with the following items:
1. One water bottle with sipper tube
2. Three luer cap plugs to seal chamber ports
3. One water bottle support bracket
4. Two 1/8” barb luer male fittings to attach tubing to the chamber
5. Four extra pneumotachograph screens
6. 24 inch length of Tygon tubing (1/8 ID) to plumb the pressure
transducer
7. One ball valve (for aerosol administration) that fits center lid port
8. One tapered stopper to seal water bottle port
9. Three 1/4” threaded hex bolt to seal lid ports

12 •Contents Plethysmograph Chamber Manual
Mouse Unrestrained Whole Body Plethysmograph Chamber
Mouse Unrestrained Whole Body Plethysmograph Chamber (600-2500-001)

Plethysmograph Chamber Manual Contents •13
Mouse Unrestrained Whole Body Plethysmograph Chamber (600-2500-001) components:

14 •Contents Plethysmograph Chamber Manual
1. Base Plate
2. Main Cylinder
3. Main Chamber Top
4. Retainer Filter Airway
5. O-Ring Silicone Red 4.193OD
6. Reference Chamber
7. Reference Chamber Top
8. Latch
9. Latch Bracket
10. Airway
11. Luer Hex to UNF
12. Bumper Rubber Black
13. Screw Pan HD 6-32x1/2
14. Screw Pan HD 4-40x3/4
15. Screw Pan HD 4-40x1.4
16. Water Bottle Adaptor
17. Screen Floor
18. Screw Pan HD 4-40x1/2
19. O Ring Silicone Red 0.50OD
20. O-Ring Silicone Red
21. O-Ring Silicone Black 0.50OD
22. Pneumotachograph Screen: SS 200x600
23. Plug Hex Male ¼” NPT
24. Dowel Pin SS: 0.062 x0.437
25. Key Way Block
28. O-Ring Silicone Red 1.00OD
29. 22MM to 1/4NPT Adaptor

Plethysmograph Chamber Manual Contents •15
The Mouse Unrestrained Whole Body Plethysmograph Chamber is packaged with the following items:
1. One water bottle with sipper tube
2. One ball valve
3. 24 inch length of Tygon tubing (1/8 ID) to plumb the pressure transducer
4. Two 1/8” barb luer male fittings to attach tubing to the chamber
5. Three luer male integral lock tings to seal chamber ports
6. Three luer male plugs to seal chamber ports
7. One tapered stopper to seal water bottle port
8. Four extra pneumotachograph screens
Unrestrained Whole Body Plethysmograph Chamber Sensors
For use with Compensated Whole Body Plethysmography respiratory method, which provides a more accurate volume
calculation.(Reference: Drorbaugh, J. E. & Fenn, W. O.: A barometric method for measuring ventilation in newborn
infants. Pediatrics. 1955; 16;81-87).
In order to use the temperature and humidity sensors the user must unscrew the two nylon plugs from the top of the
chamber and then thread the sensors into the lid taking caution not to over tighten the sensors. Over tightening the
sensors will damage the threads and cause the chamber to leak air.

16 •Contents Plethysmograph Chamber Manual
Rat Unrestrained Whole Plethysmograph Chamber with temperature and humidity sensors (circled in red)
Temperature and Humidity Sensors
•Interfaces to the 7700 UniversalXE signal conditioner.
•Attaches to the lid of the Unrestrained Whole Body Plethysmograph Chamber.
•Calibration values marked on the sensors for ease of calibrating the system.
•Different length cables are available (10 feet [3.05 meters] and 20 feet [6.1 meters]).
NOTE: The temperature and humidity sensors have different connectors on the sensor. The user should take care when
inserting the connector into the sensor to make sure that the correct cable is being used.