DSI JET Series User manual

DSI
JET User Guide
Data Sciences International
Bluetooth®
Receiver
Data Sciences International
119 14th Street NW, Suite 100
St. Paul, MN 55112
Phone: [phone]
US: [phone]
Email: [e-mail]
www.datasci.com

Data Sciences International
119 14th Street NW, Suite 100
St. Paul, MN 55112
Phone: [phone]
US: [phone]
Email: [e-mail]
www.datasci.com
Copyright1997-2012 Data Sciences International. All rights reserved. No part of this manual may be reproduced,
translated, transcribed, or transmitted in any form or by any means manual, electronic, electromagnetic, chemical, or
optical without the written permission of Data Sciences International.

DSI JET User Guide Contents
•
iii
Contents
Notices 1
FCC Notice................................................................................................................................1
Industry Canada Notice .............................................................................................................1
EU Notice –Declaration of Conformity....................................................................................1
Battery Information ...................................................................................................................2
Disclaimer..................................................................................................................................2
Product Warranty.......................................................................................................................2
Product Repairs..........................................................................................................................2
Verification................................................................................................................................2
About This User Guide 3
Related Documentation..............................................................................................................3
Theory of Operation 4
Specifications 6
JET Device Models....................................................................................................................6
JET Bluetooth® Receiver..........................................................................................................6
General Specifications...............................................................................................................6
JET Device –Technical Specifications.........................................................................6
Mechanical Specifications for JET Device..................................................................7
JET Device Battery Specifications..............................................................................7
General Specifications for JET Receiver.....................................................................7
Mechanical Specifications for JET Bluetooth® Receiver...........................................8
Lead Sets....................................................................................................................................9
Monitoring ECG with JET and a 3 Channel ECG Lead Set....................................................10
Monitoring ECG or other Bio-potentials with JET and a Differential Lead Set......................11
To Instrument a Dog for Measurement 11
Supplies Needed ......................................................................................................................11
Jacket Pocket Size Recommendations.....................................................................................12
To Attach the Electrodes..........................................................................................................13
To Install the JET Device and Connect the ECG Leads ..........................................................14
Use of JET Blood Pressure Add-On........................................................................................14
To Place Respiratory Inductive Plethysmography (RIP) Bands ..............................................15
Attaching Leads to Band Sensors ............................................................................................15
Cleaning the Sensors................................................................................................................16
Technical Specification............................................................................................................17
To De-instrument a Dog 17
Maintenance 17

iv
•
Contents DSI JET User Guide
To Charge the Battery..............................................................................................................17
To Clean the JET device and Leads.........................................................................................19
Room Setup Recommendations 20
Purpose ....................................................................................................................................20
JET Bluetooth Receiver Location Recommendations .............................................................20
Required Utilities.....................................................................................................................20
Networking..............................................................................................................................21
Overall System Integrity..........................................................................................................21
JET Bluetooth Receiver Protection Recommendations ...........................................................21
An example installation ...........................................................................................................21
Troubleshooting 22
Technical Support 24
Appendices 25

DSI JET User Guide Notices
•
1
Notices
FCC Notice
The JET subject’s device contains FCC ID QOQWT12 which complies with part 15 of the FCC Rules. Operation is
subject to the following two conditions:
1. This device may not cause harmful interference, and
2. This device must accept any interference received, including interference that may cause undesired operation.
The JET Receiver 272-0241-001 contains 3 transmitter modules with FCC ID: QOQWT141, IC ID 5123A-BGTWT41.
Device 272-0241-001 compiles with Part 15 of the FCC Rules Operation is subject t the following two conditions.
1. This device may not cause harmful interference, and
2. This device must accept any interference received, including interference that may cause undesired operation.
Each 272-0241-001 should be at least 20cm separation between the receiver and all persons.
Changes or modifications not expressly approved by the party responsible for compliance could void the user's authority
to operate the equipment. Please refer to the www.datasci.com website for more information.
Industry Canada Notice
The JET subject device contains IC: 5123A-BGTWT12A which complies with RSS 210 of Industry Canada. Operation
is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must
accept any interference received, including interference that may cause undesired operation.
The JET Receiver 272-0241-001 contains 3 transmitter modules with FCC ID: QOQWT141, IC ID 5123A-BGTWT41
which complies with RSS 210 of Industry Canada. Operation is subject to the following two conditions: (1) this device
may not cause harmful interference, and (2) this device must accept any interference received, including interference that
may cause undesired operation.
EU Notice –Declaration of Conformity
In accordance with Annex IV of the EU directive 1999/5/EC, Data Sciences International declares under our
responsibility that the JET product complies with the appropriate essential requirements of the R&TTE Directive and the

other relevant provisions, when used for its intended purpose. Please refer to the www.datasci.com website for more
information.
Battery Information
The JET device uses a removable and rechargeable Lithium Ion battery. Replacement of this battery is recommended
every 1 to 2 years depending upon storage and use conditions.
The JET RIP Modules contain a non-removable, and non-rechargeable Lithium / Manganese Oxide battery. The battery
has sufficient capacity for greater than 2700 hours of use. To maximize useful life it is recommended that the modules
be stored individually and without any cable connections.
Consult with your federal, state/province and local laws for acceptable disposal.
Disclaimer
The JET device and accessories described in this User Manual are not intended to be used and should not be used in
human experimentation or applied to humans in any way.
Product Warranty
Each JET device is warranted for 12 months. Should the JET device fail to perform within specification during the warranty
period, it will be repaired or replaced with a new unit.
Product Repairs
Repairs to the JET Device and accessories are dependent upon the amount and type of damage that is present. For example,
repair of a device due to damaged battery tabs or a damaged lead set jack is feasible. Repair of a damaged circuit board is
not feasible. .
Verification
DSI hardware and software are extensively tested and calibrated before leaving our factory or warehouse. Researchers
should independently verify the basic accuracy of materials delivered.

About This User Guide
This User Guide provides instructions on how to set up and operate the DSI JET System. The DSI JET™System is
designed to be used in conjunction with the Ponemah™ Physiology Platform (P3 Plus)/Life Science Suitesoftware for
data acquisition and analysis.
Related Documentation
Please refer to the manual for the Ponemah Physiology Platform (P3 Plus)/Life Science Suite for instructions on how to
configure and use the Ponemah software applications.

Theory of Operation
Refer to Figure 1 below.
The JET™System consists of externally worn telemetry devices. JET is a Bluetooth®enabled system specially
designed for toxicology and safety pharmacology laboratories running large animal studies. It can be used to
monitor ECG, temperature, and activity. Accessories may be used in tandem with the JET device to collect
additional endpoint (e.g. respiration and blood pressure) as described later in this document.
The use of the Bluetooth technology allows for group housing and a large number of animals per animal room.
In a JET System, surface electrodes are placed on an animal and are connected to a JET device. The JET device is
then placed into a pocket of an externally worn animal jacket. The JET device transmits to a Bluetooth®receiver.
Each Bluetooth®receiver can receive signals from up to 6 JET devices depending upon the device type. Each JET
device can transmit data up to 10 meters with negligible interference and no cross-talk exists between animals.
Bluetooth®
Receiver
Figure 1 JET System in Group Housing ConfigurationFigure 1 JET System in Group Housing Configuration

There are 3 models of JET devices available. The models differ by the number of channels that can be recorded by
each device. The housing of each of the 3 models is the same, with the devices differentiated based on the lead set
that is connected.
Figure 2 JET-3ETA-BP Device
The Bluetooth Receiver can accommodate up to six JET-EA-BP, four to six
1
JET-3ETA-BP or four JET-5ETA-BP
devices. The receiver needs to be placed in the animal room, but does not need to be in an animal cage. Multiple
receivers can be used in the same room to record from a total of 36 JET devices per room via multiple systems.
(The device limit per room for JET-5ETA-BP devices is 18.)
Figure 3 JET Bluetooth Receivers Model 2293 (left) and 272-0241-001 (right)
The JET system functions with the industry-leading Ponemah software. The Ponemah system provides continuous
data storage and experimental analysis either as they occur, or during subsequent review, with the option of
performing these actions within GLP guidelines. This solution is designed for the researcher whose protocol
demands flexibility with accurate, continuous and multi-channel acquisition and analysis in a validated environment.
The Ponemah system is modular, allowing users to custom design the configuration based upon their application,
budget and convenience —without any programming. Each Ponemah system allows up to 16 individual JET
devices to be recorded at one time though there is also a limitation of 128 channels. Through the use of multiple
Ponemah systems and multiple JET Bluetooth receives it is possible to monitor 36 animals in a single animal room
using JET-EA-BP or JET-3ETA-BP devices or 18 per room using JET-5ETA-BP devices.
1
For a small number of devices, 6 devices per receiver may be acceptable. 4 devices per receiver are recommended for optimum performance.

Specifications
JET Device Models
Refer to Figure 2.
There are 3 different models of JET devices. The main differentiator between each of the devices is the number of
channels per device. The JET-5ETA-BP device includes a second jack enabling a 2nd lead set to be connected (not
pictured).
JET-EA-BP
JET-3ETA-BP
JET-5ETA-BP
Number of ECG leads
available per device*
1
7
9
Number of BP capable
channels available per
device**
1
1
1
Respiration
Capability***
No
Yes (with 1 lead ECG)
Yes (with 7 lead ECG)
Activity
3-axis accelerometer
3-axis accelerometer
3-axis accelerometer
Temperature
No
Surface Thermistor
Surface Thermistor
*Some of the ECG channels are derived by the Ponemah P3 software.
**A JET BP Add-On is required to use this functionality.
***A JET RIP Add-On is required to use this functionality.
JET Bluetooth® Receiver
Refer to Figure 3.
The JET Bluetooth®receiver transfers data from individual JET devices to the data acquisition and analysis
software. The receiver can receive signals from up to six JET-EA-BP’s, four to six
2
JET-3ETA-BP’s or four JET-
5ETA-BP’s devices at one time.
Ponemah supports both the older JET Receiver (2293) and the new JET Receiver (272-0241-001) on the same
system at the same time.
General Specifications
The table below identifies the major specifications for the components of the JET System.
JET Device –Technical Specifications
Biopotential
Temperature
Activity
Input Range: ± 10 mV
Input Range: 0 to 70 deg. C.
Three-axis accelerometer
Sample Rate: 750 Hz
Sample Rate: 10Hz
Sample Rate: 50 Hz
Bandwidth: 0.1 to 250 Hz
Accuracy: 0.5 deg. C.
Input Impedance: > 10 M
Noise: <20 V peak-to-peak
Resolution: 16 bits
2
For a small number of devices, 6 devices per receiver may be acceptable. 4 devices per receiver are recommended for optimum performance.

Blood Pressure Add-On
Respiratory Add-On
equivalent to implant
Sample Rate: 50Hz
Resolution: 16 bits
Rate Accuracy: 5% or 2 breaths per minute
Measurement Range: 0–150 breaths per minute
Mechanical Specifications for JET Device
Length
2.5 Inches (6.35 cm)
Width
1.1 Inches (2.8 cm)
Height
3.7 inches (9.45 cm)
Total Volume
168 CCs
Weight
150 grams
Note that dimensions do not include the lead sets. The lead sets will add about 15 CCs to the total volume.
Figure 4 Dimensions of JET Device
JET Device Battery Specifications
Type
Lithium Ion
Transmission time (from full charge without BP Add-On in use)
27 hours
Transmission time (from full charge with BP Add-On in use)
25 hours
Note: See appendix for detail on care and use of these batteries over time.
General Specifications for JET Receiver
Animals Per Ponemah (P3) system
Up to 16
Transmission range between device and
receiver
10 meters
Number of JET devices per receiver
Up to 6 (dependant on device type)
Receiver connection to P3 system
Receiver can be connected directly to computer via an
Ethernet cable through a network card or into a local area

network. For more details on receiver connection to the
system, please refer to the PONEMAH Physiology Platform
user guide.
Mechanical Specifications for JET Bluetooth® Receiver
JET Receiver 2293
JET Receiver 272-0241-001
Length
8.5 inches (21.6 cm)
7.5 inches (19.1 cm)
Width
5.6 Inches (14.2 cm)
4.75 inches(12.1 cm)
Height
1.5 Inches (3.8 cm)
1.5 inches (3.8 cm)
Weight
450 grams
Figure 5 JET Bluetooth Receivers with dimensions

Lead Sets
Figure 6 Lead Set for collecting 7 leads of ECG

There are 3 different lead sets available for each of the JET devices. Each type of lead is available in versions that
are approximately 12 or 26 inches long.
A
1 channel
differential lead
set
Consists of a pair of differential leads (positive, negative) and a
reference lead.
B
3 channel
differential lead
set
Consists of 3 pairs of differential leads (positive, negative) and a
reference lead
C
3 channel ECG
Lead Set
Consists of 3 leads that share a common negative and a separate
reference lead (shown in figure 6 above)
The following table identifies the compatible lead set for each of the JET models.
JET Model
Lead sets used by the device
JET-EA-BP
A
JET-3ETA-BP
B or C
JET-5ETA-BP
Needs 2 lead sets, can be any combination of B or C
Monitoring ECG with JET and a 3 Channel ECG Lead
Set
The primary use of the JET system is to monitor ECG. The JET devices use standard ECG skin electrodes. The
color coding for the provided 3 Channel ECG lead set is based on the Association for the Advancement of Medical
Instrumentation (AAMI) standards. The following table outlines where the colored leads would be placed on an
animal.
Location
AAMI (US, Japan)
Channel Reference
Left Arm (LA)
Black
Positive Input for Channel 1
Right Arm (RA)
White
Negative Input for Channels 1, 2, and 3
Left Leg (LL)
Red
Positive Input for Channel 2
Right Leg (RL)
Green
Reference Input
Chest Lead or V lead (Vx)
Brown
Positive Input for Channel 3
As previously mentioned, Leads I and II are measured directly and the rest of the ECG leads are derived by the P3
software. The calculations used to derive the other leads are shown below.
Lead I = Channel 1
Lead II = Channel 2
Lead III = Channel 2 –Channel 1
aVR= –(Channel 1 + Channel 2) / 2
aVL= Channel 1 –((Channel 2) / 2)
aVF= Channel 2 –((Channel 1) / 2)
Vx= Channel 3 –((Channel 1 + Channel 2) / 3)
If a second 3 channel ECG lead set is used, 2 additional V leads may be calculated by the software using the
calculations below.
Vx2= Channel 5 –((Channel 1 + Channel 2) / 3)
Vx3= Channel 6 –((Channel 1 + Channel 2) / 3)
Care must be taken so as to not to mix up the Channel 1-3 and Channel 5-6 lead sets or the animal wiring will be
incorrect until the plugs are switched. Channel 4 is dedicated to the Blood Pressure jack.

Monitoring ECG or other Bio-potentials with JET and a
Differential Lead Set
Wire the animal using the positive and negative leads as needed to obtain the desired leads with no calculations or
derivations within the software.
To Instrument a Dog for
Measurement
Please Note: This user guide is not meant to function as a guide to animal care or handling. Appropriate animal
handling and care measures must be determined by the laboratory before using this device.
Supplies Needed
Item
Qty.
Comments
JET Device
1/dog
Lead Set(s)
1-2/dog
Depends on JET Model used, 2 necessary for JET-5ETA-BP
Snap Electrode Patches
3-7
Depends on lead set and JET model used.
Thermistor
1/dog
Optional
Shaving Supplies
n/a
Electric Razor, Disposable Razor, Shaving Cream, Depilatory
Misc. Supplies
n/a
Abrading Gel, Gauze Wrap
Jacket
1/dog
The jacket dimensions must be customized for your animal
The minimum jacket pocket dimensions are shown in Figure 7.
Contact your DSI Salesperson for a list of companies providing
jackets that have been optimized for use with JET devices.
Undershirt or Self
Adhesive Elastic Bandage
1/dog
A method of compressing and holding the electrodes in place and/or
holding respiratory belts in place is optional. A wrap or undershirt
can be used.
JET Respiration Add-On
1/dog
If desired, this will plug into 2 of the differential biopotential channel
inputs.
JET Blood Pressure Add-
On
1/dog
If desired, this will plug directly into the JET device.

Jacket Pocket Size
Recommendations
Refer to Figure 7.
Overall Size
The pocket in which the JET Device will be contained must have
minimum dimensions of 6” (15.2 cm) long by 2.75” (7 cm) wide by
1.25” (3.2 cm) deep in order to accommodate the JET device.
Dimensions slightly larger than those shown in Figure 7 are
recommended especially if one desires to use any JET Add-Ons such as
respiration or blood pressure.
Through Hole
The center of the one-inch diameter through hole must be at least 6”
(15.2 cm) from the bottom of the pocket and at least 1.25” (3.2 cm) from
the pocket sides. This hole allows the leads to be routed inside of the
jacket for attachment to the animal.
Figure 7 Pocket Dimensions

Pocket Location on the Jacket
The recommended pocket location is either on the back or flank depending upon the animal being used. If the pocket
is located on the back, it should be located close to the tail of the animal (with the top of the pocket toward the
head). If the pocket is located on the flank, it should be located about midway between the front and rear legs.
To Attach the Electrodes
1. Prepare the electrode and thermistor location sites as follows:
a. Shave the electrode and thermistor areas. (Refer to Figure 8)
b. Treat the shaved areas with the depilatory if necessary to denude the skin.
c. Perform any additional site preparation as required (abrasion, etc.).
d. Confirm the quality of the site preparation.
2. Place the thermistor on the animal, and then tape the thermistor in place.
3. Make sure the thermistor adheres adequately to the site.
4. Attach the electrodes to the appropriate sites according to the lead color as shown in
Figures 8a or 8b if using the 3 Channel ECG lead set for 7 Lead ECG; otherwise use your own preferred
locations for the leads desired. The diagram below exhibits the lead placement necessary for 6 leads of ECG
(Lead I, II, III, aVr, aVl, and aVf). The brown lead on the ECG lead set would be used for a V-lead
measurement. The brown lead can be placed in several different locations.
Note: It’s recommended that you avoid attaching the leads to areas of active muscle movement. This will help
minimize movement artifact. Also, if using the Respiratory Add-On avoid placing electrodes where the respiratory
belts will reside.
Figure 8a Recommended ECG Lead placement (Note: V lead not shown)
Note: Black and White electrodes are on clavicles. Green and Red electrodes are on rib cage.

Figure 8b. Alternative ECG Lead placement for dogs (without Respiration)
5. Attach the leads to the electrodes.
6. Make sure the electrodes adhere adequately to their sites.
7. Route the thermistor and electrode leads to where the jacket hole will be when the jacket is on the animal.
8. Optionally wrap the animal with a thin layer of gauze followed by a thin layer of self-adhesive elastic bandage
covering the electrode leads and wires. Make sure the leads are able to be routed through the jacket hole and to
the JET device. A snug undershirt may be used in place of the gauze and wrap if available.
To Install the JET Device and Connect the ECG Leads
1. Place the jacket onto the animal. Route the thermistor and electrode leads through the jacket hole and position
the lead plugs at the jacket pocket.
2. Connect the leads and thermistor to the JET device.
3. Make sure the back side of the JET device is facing away from the animal as instructed on the unit, then place
the unit into the jacket pocket.
4. Apply additional self-adhesive elastic bandage to hold the leads in place if necessary, then close and secure the
jacket pocket.
Use of JET Blood Pressure Add-On
In order to obtain a Blood Pressure signal into the JET Device a pressure only transmitter (e.g.
PA-C10) must be implanted into a blood vessel of the test subject and turned on. In addition, a
JET BP Add-On must be plugged into the JET Device with the antenna portion placed as close
as possible and less than or equal to 5 inches from the implanted transmitter. It is
recommended that this antenna be secured in place relative to the jacket or undershirt so that
the antenna to implant positioning is maintained throughout data collection.

To Place Respiratory Inductive Plethysmography (RIP)
Bands
1. Select the proper size band for the test subject and strap around the abdomen (above the navel and below the
ribcage) and chest (just distal to the forelimbs). Refer to Figure 9. A snug undershirt may be used if available.
2. Attach the first wire to the snap that is on the same side of the band as the
Velcro patch.
3. While holding this end to the left of the test subject’s centerline, wrap the band
around the test subject’s abdomen or chest.
4. Pull the other end over the first end and secure the band on the right side of the
centerline. The band should be snug but not too tight.
5. Attach the second wire to the exposed snap.
Figure 9 Suggested RIP
Band placement for dogs
Attaching Leads to Band Sensors
Figure 10 Abdomen and Chest Electronics Modules
A variable gain pot screw is located on the side of each electronics module. Turning the gain counter-clockwise
until a click is noticed provides the minimum gain setting. Maximum gain is achieved by turning the gain clockwise.
To determine proper gain settings start with minimum gain (full counter clockwise) and increase (turn clockwise)
until minimum useable signal is observed. Typical gain settings are 1-3 turns for beagle, 3-7 turns for cynomolgus
monkey.
Note: Each RIP Module contains a battery. As such the devices have a finite life conservatively
estimated at greater than 2700 hours of continuous use. To assure battery depletion does not occur
during storage it is recommended to have the modules disconnected from anything and stored
individually in a plastic bag.
Attach the electronics module to the RIP bands as pictured in Figure 11.
Note: Prior to connecting the cables to the modules assure the connectors are free from debris (e.g. fur)
or functionality of the RIP module could be impacted.

Figure 11 Attachment of Modules to Bands
The Chest and Abdomen modules should be connected to the 3 Channel Differential Lead as pictured in Figure 12.
The Chest Module should be connected to the White snaps and the Abdomen Module should be connected to the
Red snaps. Make sure when connecting to match polarity (i.e. + to + and - to -).
Figure 12 Attachment of Modules to 3 Channel Differential Lead Set
Cleaning the Sensors
•The bands are machine washable.
•Wipe the sensor and cable with a non-corrosive (to plastic) cleanser to clean before use.
•Make sure the complete sensor assembly is thoroughly dry before reusing it.
•To sterilize the sensors / bands, use standard gas sterilization procedures.
•Do not autoclave or soak the sensor in disinfectants.