DSI Buxco FinePointe User manual

Publication 014181-001 Rev 02
DSI Buxco® FinePointe™
Pulmonary Function Test
USER MANUAL

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Pulmonary Function Test • Publication 014181-001 Rev 02 • www.datasci.com ©2023 Data Sciences International
Table of Contents
Essential Safety Notes ...................................................................................................................................................5
Environmental Conditions.........................................................................................................................................5
Hazards and Warnings...............................................................................................................................................5
Welcome........................................................................................................................................................................7
What you will be learning .........................................................................................................................................7
Application Overview ....................................................................................................................................................8
Pulmonary Function Testing (PFT) ............................................................................................................................8
How It Works.............................................................................................................................................................9
PFT Test ...................................................................................................................................................................10
FRC (Functional Residual Capacity) Test Automation.........................................................................................10
PV (Pressure Volume) Test Automation .............................................................................................................11
FV (Flow Volume) Test Automation....................................................................................................................12
Ventilator Automation........................................................................................................................................13
Valve Sequence Automation...............................................................................................................................13
System Hardware ........................................................................................................................................................14
PFT Controller Pressure Panel .................................................................................................................................14
Pressure Panel Front...........................................................................................................................................15
Pressure Panel Back ............................................................................................................................................17
Pressure Reservoir...................................................................................................................................................18
Compressed Air Source ...........................................................................................................................................20
Chamber Manifold Design.......................................................................................................................................20
Manifold Types........................................................................................................................................................22
Mouse Manifold..................................................................................................................................................22
Rat/GP Manifold .................................................................................................................................................23
Large Animal Manifold........................................................................................................................................24
Configuring the Hardware.......................................................................................................................................25
1 - Front Panel Connections................................................................................................................................25
2 - Rear Panel Connections .................................................................................................................................26
3 - Compressed Air Source Connection ..............................................................................................................27
4 - Setting Positive Pressure................................................................................................................................28
5 - Setting Negative Pressure..............................................................................................................................29
6 - Setting Inspiration Flow .................................................................................................................................29
7 - Setting Slow Expiration Flow .........................................................................................................................30
Mouse Chamber Configuration Notes ....................................................................................................................31
Switching Between Mouse and Rat.........................................................................................................................33

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Pulmonary Function Test • Publication 014181-001 Rev 02 • www.datasci.com ©2023 Data Sciences International
Measuring Dead Space in the Manifold ..................................................................................................................34
Boyle’s Law Method............................................................................................................................................34
Alternate Boyle’s Law Method Without Patm .......................................................................................................35
Pressure Conversions and Useful Information ...................................................................................................36
Hardware Configuration in FinePointe Control Panel.............................................................................................37
System Calibration.......................................................................................................................................................42
Definitions of Signals...............................................................................................................................................42
Valves ......................................................................................................................................................................42
Calibration Procedure .............................................................................................................................................43
1 - Open the Calibration Window .......................................................................................................................43
2 - Calibrate Flows...............................................................................................................................................45
3 - Calibrate Pressure..........................................................................................................................................50
Creating a PFT Study....................................................................................................................................................53
Acquiring Data .............................................................................................................................................................56
Launching Acquisition .............................................................................................................................................56
Loading the subject .................................................................................................................................................60
Saving Your Data .....................................................................................................................................................61
Reports ........................................................................................................................................................................62
Creating and Modifying Groups ..............................................................................................................................62
Creating Reports......................................................................................................................................................64
Modifying Measurements.......................................................................................................................................66
Rejecting Data .........................................................................................................................................................69
Test Results..................................................................................................................................................................69
FRC ..........................................................................................................................................................................70
Quasistatic Pressure Volume...................................................................................................................................70
Fast Flow Volume ....................................................................................................................................................71
Resistance & Compliance ........................................................................................................................................72
Appendix A - Troubleshooting .....................................................................................................................................73
Common Reasons PFT Tests Might Fail...................................................................................................................73
FRC Test ..............................................................................................................................................................73
PV Test ................................................................................................................................................................73
FV Test ................................................................................................................................................................74
Leaks........................................................................................................................................................................ 74
Common Symptoms............................................................................................................................................74
General Suggestions ...........................................................................................................................................74
Test for Leaks in the Main Chamber ...................................................................................................................75

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Pulmonary Function Test • Publication 014181-001 Rev 02 • www.datasci.com ©2023 Data Sciences International
Test for Leaks in the Valve Assembly..................................................................................................................75
Animal Breathes During Tests .................................................................................................................................76
Appendix B – Anesthesia .............................................................................................................................................77
First Choice..............................................................................................................................................................77
Second Choice .........................................................................................................................................................78
Third Choice............................................................................................................................................................. 78
Notes .......................................................................................................................................................................78
Appendix C - Interpretations PFT Results ....................................................................................................................79
FRC (Functional Residual Capacity) Test..................................................................................................................80
Occlude at FRC ....................................................................................................................................................80
No Efforts During FRC Occlusion.........................................................................................................................81
Data Assumptions Met .......................................................................................................................................81
PV (Pressure Volume) Test......................................................................................................................................82
Test Starts When Flow Is Zero ............................................................................................................................82
At TLC, Volume Rises as Pressure Falls ...............................................................................................................82
At TLC, Volume Drops as Pressure Falls..............................................................................................................83
Almost Vertical Volume Drop Immediately after the Hold Period .....................................................................83
Expiratory Duration Should be Long Enough......................................................................................................83
Volume Should Rise Steadily During Inspire to TLC ............................................................................................83
Volume-Pressure Curve is Smooth .....................................................................................................................83
Overlays of Consecutive PV Tests Differ .............................................................................................................84
FV (Flow Volume) Test ............................................................................................................................................85
Ripples or Squiggles Near the End of the Flow-Volume Curve ...........................................................................85
A Flat Top of the Flow Volume Curve .................................................................................................................85
A Dip in the Center of the Flow-Volume Curve...................................................................................................85
Generally Rounded Flow-Volume Curve.............................................................................................................85
FVC Value is Wildly Different Than IC .................................................................................................................85
Appendix D - Suggested Reading.................................................................................................................................86
Contact Information ....................................................................................................................................................87
DSI Technical Support—North America..............................................................................................................87
DSI Technical Support—Europe..........................................................................................................................87

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Pulmonary Function Test • Publication 014181-001 Rev 02 • www.datasci.com ©2023 Data Sciences International
Essential Safety Notes
This section describes potential hazards which may exist in the operation of these units. A number of warning
labels and symbols are affixed to your instrument. These symbols are used to inform you of potential dangers
which may exist or where caution is required.
THE PROTECTION GIVEN BY THE EQUIPMENT MAY BE IMPAIRED IF USED IN A MANNER NOT SPECIFIED BY THE
MANUFACTURER.
Environmental Conditions
•Indoor use
•Altitude up to 2000 m
•Storage and operation range: 4°C to 40°C; 10% - 80% Rh, Non-condensing
•Mains supply voltage fluctuations not to exceed ± 10% of the nominal voltage
•Supply voltage, voltage range: 12vdc, 4.0A
•Overvoltage Category 2
•Pollution degree 2
Hazards and Warnings
This instrument is subject to the following identified hazards:
CAUTION: The controller is heavy, to transport the controller use two people grasping either end of the controller and lift
using proper techniques.
The AC/DC adapter requires connection to protective earthing (ground), only use with a power supply outlet and power supply
cord that provides protective earthing.
Only use a detachable power cord (AC/DC adapter) that allows for protective earthing and is a minimum of 18 AWG. This cord
will need to have appropriate agency approvals, such as UL, CSA.
DSI cannot guarantee the safety of this device if used other than intended or used by any procedures other than those described
in this manual.
The AC/DC adapter requires connection to protective earthing (ground), only use with a power supply outlet and power supply
cord that provides protective earthing.
Only use a detachable power cord that allows for protective earthing and is a minimum of 18 AWG. This cord will need to have
appropriate agency approvals, such as UL, CSA.
Do Not Operate with Suspected Failures
If damage is suspected on or to the product, do not operate the product. Contact qualified service personnel to perform
inspection.

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Pulmonary Function Test • Publication 014181-001 Rev 02 • www.datasci.com ©2023 Data Sciences International
Orient the Equipment Properly
Do not orient the equipment so that it is difficult to manage the disconnection device.
Place Product in Proper Environment
Review the operating manual for guidelines for proper operating environments.
Observe all Warning Labels on Product
Read all labels on product to ensure proper usage.
Before installing your new unit, please take time to familiarize yourself with these warnings and symbols:
Direct Current (DC)
Caution

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Pulmonary Function Test • Publication 014181-001 Rev 02 • www.datasci.com ©2023 Data Sciences International
Welcome
Congratulations on joining the community of users worldwide who rely on DSI’s products to perform preclinical
physiologic research. Thank you for your interest in DSI products. We are committed to providing you with quality
products and services.
This manual will help you get to know your FinePointe Pulmonary Function Test system. The structure of the
manual was designed to sequentially guide you through using your DSI system from set up to data acquisition.
What you will be learning
1. Understand how respiratory data is collected using the Pulmonary Function Test system.
a. Hardware
b. Software
2. How to setup your Pulmonary function Test system hardware.
3. How to use the FinePointe software to:
a. Create a hardware configuration
b. Create a study
c. Calibrate hardware
d. Acquire data
e. Generate reports
f. Review data

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Pulmonary Function Test • Publication 014181-001 Rev 02 • www.datasci.com ©2023 Data Sciences International
Application Overview
Pulmonary Function Testing (PFT)
This analyzer monitors a respiratory flow signal and any airway pressure signal during a succession of forced
pulmonary tests from an anesthetized test subject. These tests offer a unique and comprehensive look at the
function of the lung.
PFT is an acronym for Pulmonary Function Testing. This system is also known as Pulmonary Maneuvers. The PFT
system automates a series of tests on an animal which simulate clinical pulmonary testing routinely performed on
humans.
There are 3 tests that the PFT system can perform: FRC, PV, and FV. In addition, the system allows the researcher
to acquire baseline resistance and compliance data.
This picture below illustrates all the lung volumes.
Lung Volumes

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Pulmonary Function Test • Publication 014181-001 Rev 02 • www.datasci.com ©2023 Data Sciences International
The FRC tests measures the FRC lung volume by using Boyle’s Law. FRC lung volume is the Functional Residual
Capacity. This is the volume of air that remains in the lung at the end of a normal expiration. This lung volume is
important because by knowing this lung volume, it is easy to know all the other lung volumes.
See Section FRC for list of common parameters reported.
The PV test allows the researcher to know the static lung properties. Static lung properties are described by the
pressure-volume relationship of the lung. The slope of the Volume-Pressure curve is the instantaneous compliance
at a given pressure. See Section Quasistatic Pressure VolumeFRC Test for list of common parameters reported.
The FV test allows the researcher to know the dynamic lung properties. Dynamic lung properties are described by
the flow-volume relationship of the lung. The Flow-Volume curve shows the flow at a given expired volume. Since
the pressure across the lung is essentially constant during the acquisition of the flow-volume data, the flow value is
proportional to the conductance of the limiting airways at a given expired volume. See Section Fast Flow Volume
for list of common parameters reported.
See Section Resistance & Compliance for list of common parameters reported.
How It Works
The subject has a tracheotomy performed (or is intubated) and placed in the supine position on the bed within the
plethysmograph. The trach tube is connected to the face plate where fresh air is provided either via bias flow or
through ventilation. The face plate contains a manifold of valves which are actuated to perform the PFT tests.
Also, supporting the manifold is a pressure panel which provides flows at safe pressures to the manifold. The
pressure panel also contains preamplifiers necessary to condition the flow and pressure signals needed for the
data analysis.
With the plethysmograph placed around the subject, the subject breathes through the wall of the face plate and
the chest expands with the air entering and contracts as the air exits the lungs. This expansion forces an equal
amount of air out of the plethysmograph through a screen pneumotach. The pneumotach resists the movement
of air, and so, pressure in the chamber is created as a result of the air being forced through the pneumotach. This
pressure is linearly related to flow of air passing through the pneumotach, and since that is the same as the
volume inspired by the subject, the pressure is related to the flow in and out of the subject.
The lung pressure is measured in the tracheal line. Normally, the pressure is referenced to atmosphere, but it can
optionally be referenced to the subject’s esophagus or even to the intrapleural cavity using a needle.

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Pulmonary Function Test • Publication 014181-001 Rev 02 • www.datasci.com ©2023 Data Sciences International
PFT Test
The following summarizes the test automation:
FRC (Functional Residual Capacity) Test Automation
•Uses Boyle’s Law to determine FRC
•Synchronize to Breathing
•Occlude airway until the subject attempts to breathe on its own