Eicom AZ-X-Y User manual

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User’s ManUal
Concentric Microdialysis Probe
Model AZ-X-Y
• AllEicomproductsareforanimalresearchpurposeonly.
• Keeptheprobeawayfromphysicalshock.
• Inletneedleislongerthanoutletneedle.
• Duramustbebrokenpriortoinsertionintothebrain.
AZ-X-Y Probe
the probe membrane. After flushing for several minutes (it is not
necessarytoflushforanextendedperiodoftime)themembranemay
“sweat”orappearwetthisisnormal,notduetodamageoraclog.
2. Ifnoflowisobservedviatheoutletneedle,soaktheprobeindistilled
water for a few minutes and repeat instructions in item 1. After
conrmingthedistilledwaterflowfromtheoutlet,pleaseremovethe
probefromplasticsheathandconrmnoleaking.
3. We test each probe for defect/leaking prior to shipping. If you nd
adefectiveprobeafterfollowingtheabovestepsEicomwillreplace
theprobeatnocharge.Wedonotguaranteeprobesafterinsertion
toanimals.
Removing Air Bubble
1. Ifthereisanairbubbleintheprobe,pleaseremoveit.Becausethe
airbubblewillcovertheactivemembranesiteandkeeptheperfusate
away.Thiswillresultinalowerrecoveryrate.Theairbubbleshouldbe
removedbeforestudy.Ifitissmall,itwilldisappearincaseofinvivo
studybutmaynotinvitro.
2. Perfusateneedstobedegassedbeforeuse.
3. Toremovetheairbubble,pleaseintroduceabigairtotheinletand
flushout.Donotconnectoutlettubingatthistime.Thebigairbubble
willtrapandremoveasmallairbubble.
Connecting Outlet
1. Conrmneitherleakingorairbubblesarepresentinthemembrane.
Settheflowrateonthesyringepump.Astandardflowrateis1-2μl/
min.Ahighflowratecoupledwithalongoutlettubingcanresultin
damageoftheprobemembrane.
2. Connect Teflon tubing (model JT-10) or other tubing to the outlet.
A long tubing length (more than 150 cm) can cause leaking of the
membraneortheliquidswivelduetotheinducedhighpressure.Ifyou
usetubinglongerthan150cm,pleaseavoidusingflowrateshigher
than 2 μl/min. Polyethylene tubing can cause confounding peaks
whenanalyzingsamplesusingHPLCwithelectrochemicaldetection.
Cleaning Probes and Storage
Removetheprobefromthesystemandflushwithdistilledwatertowash
out salts. Then put the probe in the distilled water and place at 4°C for
storage. Keep the membrane wet to prevent itfrom shrinking. Keep the
probeawayfromphysicalshock.
Cleaning Lines (Teflon, FEP tubing)
Bacteriagenerationandproteinpollutionoftubingcanbeacauseoflow
recoveryrates,especiallyintheoutlettubing.Inordertoavoidthisproblem,
washthelineswith50timesdilutedcommercialkitchenbleach(chlorine)in
purewater.Perfusethesolutionintothelines(nottheprobe)andleaveitfor
atleastonehour,thenthoroughlyrinsewithwater.
Perfusion Solution
Wearecommendusinganon-bufferedaCSFsolutionbecausephosphate
buffersolutionsmayprecipitatecalciumphosphateandcanresultinclogs.
Probe Check
1. Injectdistilled water using a1 or 2 mldisposable (plastic) syringe.
Connect the syringe to the inlet (longer needle) of the probe using
Bitontubing(JB-30).Theprobemaybecomecloggedordamagedif
youinitiallyflushwithasaltsolution.Werecommendusingdistilled
waterfortheinitialprobeflush.Donotconnecttubingtotheoutlet
oftheprobeduringflushing.Pleaseconrmtherearenoleaksfrom
AD12-0718
inner 0.105/0.040 mm PFS
outer 0.63/0.33 mm SUS
inner 0.150/0.075 mm PFS
outer 0.63/0.33 mm SUS
0.3/0.2 mm SUS
0.18/0.12 mm SUS
Inner Dead Volume
inlet: 0.05 µL
outlet: 0.4 µL
9.0 mm
12.0 mm
X + 5
PFS: Polyimide-Resin
Coated Fused Silica
Membrane: Artificial Cellulose
Cut off: MW 50,000
OD/ID: 0.22/0.20 mm
SUS (Stainless Steel) 316
Y
Model: AZ-X-Y
X = (guide cannula length)
Y = (membrane length)

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Cap Nut
(AC-1)
Guide Cannula
(AG-X)
Stero Adapter
(SAG-X)
Clamp of
Stereotaxic Apparatus
not provided from Eicom
Guide Cannula crown is
acrylic resin made and
adhesive to acrylic
dental cement but Cap
Nut is not acrylic resin.
Stero Adapter
(SAG-X)
Dental Cement
Ancor Screw (AN-3)
After confirming fixation of the guide
cannula, loose the cap nut and remove stero
adaptor from the guide cannula. Instead of
the stero adaptor, insert the dummy cannula
(AD-X) and fix with a cap nut.
Dummy Cannula (AD-X)
CBA
ED
CongurationofStereoAdaptor(SAG-X),GuideCannula(AG-X),DummyCannula(AD-X)&CapNut(AC-1)
USA
10060CarrollCanyonRd.,Ste100,SanDiego,CA92131
TollFree(888)680-7775Fax(858)560-8040
Europe
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Phone+35319022700Fax+35314430784