Elisabeth Pharmacon Zephyrus Gel Box Enclosure 2.0 Series User manual

Zephyrus Gel Box Enclosure 2.0
Instructions for Use
Ref. No.: Z-DOC-504-2
Created by: VIJU Instruction Manual ZEPYHRUS® Gel Box Blue System 2.0
Source: https://www.elisabeth.cz/en/product-zephyrus-gel-box-blue-system-20.html
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Content
Introduction .....................................................................................................................................3
Warnings ..........................................................................................................................................3
Unpacking.........................................................................................................................................3
Setting up Zephyrus Gel Box Enclosure 2.0 .....................................................................................4
Photo Filters.....................................................................................................................................5
Gel Imagining ...................................................................................................................................5
Technical Specification.....................................................................................................................7
Accessories and Consumables .........................................................................................................8
Warranty ..........................................................................................................................................8
Created by: VIJU Instruction Manual ZEPYHRUS® Gel Box Blue System 2.0
Source: https://www.elisabeth.cz/en/product-zephyrus-gel-box-blue-system-20.html
This file is owned by ELISABETH PHARMACON, spol. s r. o., It is issued for publication. Version: 240220-01
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Introduction
Congratulations on your purchase of a Zephyrus Gel Box Enclosure 2.0. This enclosure is designed
for capturing high quality photos of electrophoresis gels with a smart phone or tablet camera. The
Zephyrus Gel Box Enclosure 2.0 can be used on existing blue light or UV transilluminators.
An orange photo filter is included with the system. This filter is designed to absorb (block) blue
excitation light from a blue light transilluminator, allowing through only the fluorescing sample
light for capturing gel images using a smart phone camera.
Additional filters are required and available separately for imaging gels on a UV transilluminator.
To ensure proper operation and performance from your Zephyrus Gel Box Enclosure 2.0, please
read this manual in its entirety before use.
Warnings
Read this manual in its entirety before operating the Zephyrus Gel Box Enclosure 2.0, and keep the
manual for future reference.
CAUTION: When using blue light transilluminators: Although blue light does not pose
the same hazards as UV light, the emitted blue light is very bright. It is not
recommended to look directly at the blue light illumination surface.
CAUTION: When working in a laboratory environment, it is always important to
properly handle all reagents and chemicals. When using a cell phone for capturing
gel images, it is very important not to contaminate the phone with any chemicals or
reagents. Use proper and common laboratory safety practices when working with reagents and
gels stained with DNA dye. Always wear gloves, and always change gloves when switching from
handling gels, or reagent to use of a cell phone, and vice versa. Zephyrus Gel Box Enclosure 2.0
components should be cleaned periodically using mild soap and water or 10% bleach solution for
decontamination.
CAUTION: If using Zephyrus Gel Box Enclosure 2.0 on a UV transilluminator, always
use caution to prevent exposure of the skin and eyes to UV light. If working on a UV
transilluminator with a viewing surface larger than the bottom of the Zephyrus Gel
Box Enclosure 2.0, use the GBE UV Blocking Mat to block UV light from emitting around the edges
of Zephyrus Gel Box Enclosure 2.0.
Unpacking
NOTE: Always handle the Zephyrus Gel Box Enclosure 2.0 and parts with care.
Carefully remove the system and all accessories from the carton and remove any foam protective
pieces. Check for any shipping damage. In the event of shipping damage, a claim must be filed with
Created by: VIJU Instruction Manual ZEPYHRUS® Gel Box Blue System 2.0
Source: https://www.elisabeth.cz/en/product-zephyrus-gel-box-blue-system-20.html
This file is owned by ELISABETH PHARMACON, spol. s r. o., It is issued for publication. Version: 240220-01
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the carrier. Check the contents of the package to make sure you have received all of the parts
ordered:
•Zephyrus Gel Box Enclosure 2.0: 1) Main enclosure hood, 2) extending adapter, and 3) top
platform for smartphone.
•Orange Photo Filter
•Any additional filters for UV (ordered separately)
If any parts are damaged or missing, please immediately contact Elisabeth Pharmacon Ltd. at +420
542 213 851 or email [email protected]
IMPORTANT: Save the carton and packing materials for storing and transporting Zephyrus Gel
Box Enclosure 2.0 or returning it for any required servicing.
1. Zephyrus Gel Box Enclosure 2.0 Assembly, Overview of Parts
Setting up Zephyrus Gel Box Enclosure 2.0
Assemble the components of Zephyrus Gel Box Enclosure 2.0 as pictured in section above of this
manual. Depending on the size of the gels to be imaged, or the desired detail of the image, the
extender piece can be connected or removed. The top, smart phone platform piece connects
either to the top of the Extender or to the top of the Main Enclosure. These parts will snap
together and pull apart with gentle pressure.
Smart phone
platform
Pla
Removable
extender
(remove for close)
up) imaging)
Orange Photo filter
Main Enclosure
Hood
Created by: VIJU Instruction Manual ZEPYHRUS® Gel Box Blue System 2.0
Source: https://www.elisabeth.cz/en/product-zephyrus-gel-box-blue-system-20.html
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Photo Filters
A photo filter is required to capture quality images with the Zephyrus Gel Box Enclosure 2.0. The
GBE Filter Orange is included and this filter blocks the visible blue wavelengths coming from the
illumination base or other blue light transilluminators. It allows through the emission wavelengths
from fluorescing nucleic acid stains, to allow clear pictures of gel bands.
Additional filters are available separately for the Zephyrus Gel Box Enclosure 2.0 and are
recommended when using the enclosure on a UV Transilluminator. These glass, band pass filters
block UV light so the UV bulbs will not be seen in the images, and they also eliminate background
wavelengths from the excitation source.
Z-DOC-521-G
band pass photo filter, 590nm, for imaging EtBR on UV transilluminator
Z-DOC-521-B
band pass photo filter, 535nm for imaging green stains on UV transilluminator
Caution: Using the Zephyrus Gel Box Enclosure 2.0 on a UV Transilluminator without
an appropriate filter in place can cause eye or skin exposure to UV radiation. The
Zephyrus Gel Box Enclosure 2.0 filters effectively block harmful UV radiation, and
should always be properly installed when using UV illumination.
Gel Imagining
Electrophoresis gels should be prepared and stained according to the instructions included with
the nucleic acid stain. Use the proper type of transilluminator for the stain used. Common stains
that work well with the Zephyrus Gel Box Enclosure 2.0 enclosure and filters include:
EliDNA™ PS Green, EliDNA™ PS Green Plus, EliDNA™ PS Green Ultra, EliDNA™ LD Green SYBR®
Green I and II, SYBR® Safe, SYBR® Gold, Gel Star, Gel Green, and Green Glo™, Ethidium Bromide,
Gel Red.
Z-DOC-521-O
(included)
Z-DOC-521-B
(optional)
Z-DOC-521-G
(optional)
Created by: VIJU Instruction Manual ZEPYHRUS® Gel Box Blue System 2.0
Source: https://www.elisabeth.cz/en/product-zephyrus-gel-box-blue-system-20.html
This file is owned by ELISABETH PHARMACON, spol. s r. o., It is issued for publication. Version: 240220-01
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1. The UV or blue light transilluminator should be set up on a level and stable lab bench or
table.
2. Carefully place a stained electrophoresis gel onto the transilluminator viewing surface.
3. Note: The gel to be imaged should be 15x15cm or smaller. Cut the gel to a smaller size if
required.
4. Note: If the transilluminator being used has a viewing surface larger than 19x19cm, please
use GBE UV Blocking Mat. This will block any UV or blue light from coming around the
edges of the SmartDoc enclosure base.
5. Place the Zephyrus Gel Box Enclosure 2.0 imaging enclosure onto the transilluminator.
6. Insert the appropriate filter into place on the top platform. The side with the Zephyrus logo
should be facing up. See Section 6 for information on filters.
7. Place a smartphone face down onto the top platform and align the camera lens with the
filter. NOTE: Some protective phone cases can prevent the camera lens from aligning and
connecting to the photo filter. Remove the phone case if necessary.
8. Turn on the power of the transilluminator.
9. Select the Camera Mode on your Smart Phone and turn off the flash setting. When
properly positioned, the gel will be seen in the device’s display screen. Focus as required.
10. If required, remove or insert the extender piece to maximize the image size of the gel in
the display. NOTE: Always turn of the power of the transilluminator when adjusting the
Zephyrus Gel Box Enclosure 2.0.
A zoom function on the camera phone can be used to enlarge the gel image in the display, but
this can decrease the resolution of the image.
Created by: VIJU Instruction Manual ZEPYHRUS® Gel Box Blue System 2.0
Source: https://www.elisabeth.cz/en/product-zephyrus-gel-box-blue-system-20.html
This file is owned by ELISABETH PHARMACON, spol. s r. o., It is issued for publication. Version: 240220-01
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Technical Specification
Maximum Gel Size:
15 x 15 cm
Included imaging filter:
Orange PMMA, 12mm aperture
Dimensions (WxDxH):
23x19x22 cm
Weight:
0.9 kg
Phone compatibility:
iPhone, Samsung, LG, HTC smartphones and tablets with
camera
Zephyrus Gel Box Enclosure 2.0 properly
positioned on a UV Transilluminator.
Note: On some models of transilluminators,
the protective cover may need to be removed.
Zephyrus Gel Box Enclosure 2.0properly
positioned on a Blue Light Transilluminator.
Created by: VIJU Instruction Manual ZEPYHRUS® Gel Box Blue System 2.0
Source: https://www.elisabeth.cz/en/product-zephyrus-gel-box-blue-system-20.html
This file is owned by ELISABETH PHARMACON, spol. s r. o., It is issued for publication. Version: 240220-01
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Accessories and Consumables
Item No.
Description
Z-ILU-505-UV
UV Transilluminator, 302nm, 16 x 19 cm viewing surface
Z-ILU-505-B
Blue Light Transilluminator, 465nm, 12 x 17cm viewing surface
Z-DOC-521-O
Orange photo filter for use with blue light illuminators
Z-DOC-521-G
band pass photo filter, 590nm, for imaging EtBR on UV transilluminator
Z-DOC-521-B
band pass photo filter, 535nm for imaging green stains on UV transilluminator
Z-DOC-520
GBE UV Blocking Mat
ED01
EliDNA™ PS Green, 1.0ml
ED01s
EliDNA™ PS Green, Sample, 50ul
ED02
EliDNA™ PS Green Plus, 1.0ml
ED02s
EliDNA™ PS Green Plus, Sample, 50ul
ED03
EliDNA™ PS Green Ultra, 1.0ml
ED03s
EliDNA™ PS Green Ultra, Sample, 50ul
ED05
EliDNA™ LD Green, 1.0ml
ED05s
EliDNA™ LD Green, Sample, 50ul
Warranty
The Zephyrus Gel Box Enclosure 2.0 is warranted against defects in materials, workmanship and
normal use for 1 year. If any defects occur in the instrument or accessories during this warranty
period, Elisabeth Pharmacon Ltd. will repair/replace the defective parts/replace the instrument at
its discretion without charge. Warranty is excluded in tha case of following:
-unauthorized repair
-use of spare parts by anyone other than Elisabeth Pharmacon Ltd.
-used in the manner not described in this manual
-damage caused by accident, neglect, misuse, improper service, natural forces or other
causes not arising from defects in original material or workmanship
Manufacturer:
ELISABETH PHARMACON Ltd.
Rokycanova 4437/5, Brno-Židenice 615 00
[email protected] | www.elisabeth.cz | tel.: [phone]
Created by: VIJU Instruction Manual ZEPYHRUS® Gel Box Blue System 2.0
Source: https://www.elisabeth.cz/en/product-zephyrus-gel-box-blue-system-20.html
This file is owned by ELISABETH PHARMACON, spol. s r. o., It is issued for publication. Version: 240220-01
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