SynOptics SYNBIOSIS ProtoCOL 3 User manual

ProtoCOL 3
2-Sector Spiral Batch Set Up - Quick Guide
Start ProtoCOL 3 software
Log on to ProtoCOL 3
Capture image
Select the image tab and check the
live box
Create batch
Click New Batch
Position plate
Insert the plate holder, ensuring the
plate holder that gives the best
contrast between the colony and
background is used
Adjust the exposure by increasing/
decreasing the sliding scale
Capture image
Click
Click
Enter
Slide
Click
Plate configuration Select Plate Configuration to set
plate size, sample volume and
threshold. Defaults are Circular
plate 90mm, Spiral volume
100µl and threshold of 20.
Change if necessary
Select
Select
Choose an application
Select Spiral Plate
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Place plate onto plate holder
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Total plate count
Position the counting frame over
the colonies to be counted
Click
ProtoCOL 3 is now ready to make the first measurement
Accept batch
Select
Click Click Total Plate Count
Select colony type from the
drop-down, either Light or Dark
Colonies
Slide
Check
Move slider to the left to
accept small particles or to the
right to exclude small particles
If any colonies are touching
select the Split function
Classification settings Move slider to the left to adjust
the detection sensitivity
Enter a plate ID
Enter
Enter a plate ID found on
Measure tab. Auto-increment
default is “on”, to change this
click on the Configuration tab
Click
Click
Accept batch
Name the batch
Enter Name the batch
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Email: [e-mail] Email: [e-mail]
www.synbiosis.com P32SSBSU1216
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