Evolve 70 Manual

70mm, 100mm and 140mm
Chromatography Columns

DELTA PRECISION LTD
Chromatography Column
Operating Manual and Installation Guide
70mm, 100mm and 140mm Columns

Revision History
Revision
Comments
Date
A
Initial release
21/11/2014
B
Photos updated
25/02/2015
C
Address Updated
21/06/2018
D
Pressure Equipment Directive updated
06/04/2021
E
Technical specification updated caster not available on 70-140mm columns
22/04/2021
F
Updated to reflect Evolve® Trademark status
12/07/2021
Evolve® is a registered trademark of Delta Precision Ltd. Evolve® is a registered trademark in the
EU, UK and US.
Manual Revision
Date
F
July 2021

Table of Contents
Column Hardware .......................................................................................................... 1
Mechanical Description ........................................................................................................................... 1
Technical Data. ...................................................................................................................................... 3
Weights and Dimensions ........................................................................................................................ 3
Pressure Drop ........................................................................................................................................ 4
Material Conformity ............................................................................................................................... 6
Chemical Compatibility .......................................................................................................................... 7
Safety Guidelines ............................................................................................................ 9
Pressure Equipment Directive (PED). ................................................................................................... 9
Over-pressurization ................................................................................................................................. 9
Handling ................................................................................................................................................ 9
Chemical compatibility .......................................................................................................................... 10
Installation and Column Set-up ................................................................................... 11
Installation. .......................................................................................................................................... 11
Column Operation ........................................................................................................ 15
Hydrostatic Leak Test ......................................................................................................................... 15
Media Preparation ............................................................................................................................... 16
Column Packing (Constant Flowrate Method) ...................................................................................... 16
Unpacking ........................................................................................................................................... 18
Cleaning and Storage ............................................................................................................................ 18
Maintenance ................................................................................................................. 20
Routine Maintenance Procedures-General guidelines .............................................................................. 20
Disassembly of 70mm, 100mm and 140mm Columns ......................................................................... 21
Reassembly of 70mm, 100mm and 140 column. ................................................................................... 24
Troubleshooting ........................................................................................................... 27
Leakage from adjuster seal .................................................................................................................... 27
Leakage from fixed bed support seal ..................................................................................................... 27
Leakage from inlet or outlet .................................................................................................................. 27
Excessive column back pressure ............................................................................................................ 28
Media Leakage .................................................................................................................................... 28
Kits, Spare parts and Accessories ................................................................................. 29
Spare parts and kits ............................................................................................................................. 31
Spare Parts and kits Cont. ................................................................................................................... 32
Accessories ............................................................................................................................................ 34
Contact Us .................................................................................................................... 35
Appendix 1 Chromatography Performance .................................................................. 36
Performance Measurement and Column Efficiency ................................................................................. 36
Performance Results From Evolve®Columns ........................................................................................ 38

Table of Figures
Figure 1.1 Evolve®Column Range 70mm, 100mm, 140mm, 200mm and 250mm columns .................... 2
Figure 1.2. Pressure Drop Evolve®70mm column ..................................................................................... 4
Figure 1.3 Pressure Drop Evolve®100mm column ................................................................................... 4
Figure 1.4 Pressure Drop Evolve®140mm column ................................................................................... 5
Figure 3.1 Diagram showing the location of the fixed bed support ............................................................. 11
Figure 3.2 Photograph showing the installation of the fixed bed support .................................................... 12
Figure 3.3 Diagram showing the location of the adjuster bed support ........................................................ 13
Figure 3.4 Photograph showing the location of the adjuster bed support ..................................................... 13
Figure 4.1 Schematic of the column with part identification ....................................................................... 17
Figure 5.1 Adjuster Assembly Evolve® 100 column .............................................................................. 22
Figure 5.2 Fixed Assembly Evolve® 100 column ................................................................................... 23
Figure 5.3 Diagram showing the location of the fixed bed support ............................................................. 24
Figure 5.4 Photograph showing the installation of the fixed bed support .................................................... 24
Figure 5.4 Photograph showing the location of the adjuster bed support ..................................................... 25
Figure 5.5 Diagram showing the location of the adjuster bed support ........................................................ 25
Figure 5.6 Photograph showing the final location of the adjuster bed support ............................................. 26
Figure 5.7 Photograph showing the alignment of marking on flowtube and adaptor to ensure component is
correctly tightened ....................................................................................................................... 26
Figure A1 Description of Column Efficiency Calculations ........................................................................ 37
Figure A2 Example of typical chromatogram. Evolve®250mm column. 2% Acetone sample, flowrate
100cm/hr, bed height 19.5cm. ................................................................................................... 38