Fisherbrand Basic 60L User manual

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version of these operating instructions is binding.
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Thermo Fisher Scientific
300 Industr Drive
Pittsburgh, Penns lvania 15275
United States
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The content of this document are subject to change without notice.
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supersede all previous information received b the purchaser.
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govern or modif an Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information
between the two documents.

Fisherbrand 50152755 | 1
Table of Contents
Chapter 1 Table of Contents .....................................................................................................................................................1
Chapter 2 Safety Notes.............................................................................................................................................................1
Basic Operating Precautions........................................................................................................................................1
Operational Safet Rules .............................................................................................................................................2
Warrant ....................................................................................................................................................................2
Explanation of Safet Information and S mbols .............................................................................................................3
.............................................................................................................................................................................3
Additional S mbols for Safet Information ................................................................................................................3
S mbols on the Incubator........................................................................................................................................4
Intended Purpose of the Incubator ...............................................................................................................................5
Correct Use............................................................................................................................................................5
Incorrect Use..........................................................................................................................................................5
Standards and Directives ............................................................................................................................................5
Chapter 3 Delivery of the Incubator ..........................................................................................................................................
Packaging..................................................................................................................................................................7
Acceptance Inspection ................................................................................................................................................7
Scope of Suppl .........................................................................................................................................................8
Incubators..............................................................................................................................................................8
Chapter 4 Installation ...............................................................................................................................................................9
Ambient Conditions ....................................................................................................................................................9
Location Requirements ...........................................................................................................................................9
Intermediate Storage ................................................................................................................................................10
Room Ventilation ......................................................................................................................................................10
Incubator Dimensions and Clearances ...................................................................................................................11
Transport.............................................................................................................................................................12
Installing the Anti-tilt Anchor .....................................................................................................................................13
Chapter 5 Product Description................................................................................................................................................15
Basic Gravit Incubator Overview ...............................................................................................................................15
Work Space Atmosphere...........................................................................................................................................16
Sensing and Control S stem......................................................................................................................................17
AC Power Socket..................................................................................................................................................17
Fuses ..................................................................................................................................................................17
Work Space Components..........................................................................................................................................18
Inner Chamber .....................................................................................................................................................18
Access Port..........................................................................................................................................................18
Shelf S stem........................................................................................................................................................19
Chapter 6 Start-up..................................................................................................................................................................21
Installing the Shelf S stem ........................................................................................................................................21
Initial Installation.......................................................................................................................................................21
Preparing the Work Space.........................................................................................................................................22
Installation of the Support Rails .............................................................................................................................22
Installing the Shelf Support Brackets......................................................................................................................23
Installing the Perforated Shelves............................................................................................................................23
Levelling the Incubator..........................................................................................................................................24

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| 50152755 Fisherbrand
Connecting Power ....................................................................................................................................................24
Connecting to the Power Suppl Source.................................................................................................................24
Electrical Requirements to be Met when Operating the Incubators in the European Union:.............................................25
Chapter Operation................................................................................................................................................................2
Preparing the Incubator.............................................................................................................................................27
Device Check .......................................................................................................................................................27
Starting Operation ................................................................................................................................................27
Chapter 8 Handling and Control..............................................................................................................................................29
Overview..................................................................................................................................................................29
Operator mode.....................................................................................................................................................30
Calibration of the reference sensor ........................................................................................................................31
Chapter 9 Shut-down .............................................................................................................................................................33
Shutting the Incubator Down .....................................................................................................................................33
Chapter 10 Cleaning and Disinfection ......................................................................................................................................35
Cleaning ..............................................................................................................................................................35
Cleaning Exterior Surfaces ....................................................................................................................................35
Wipe / Spra Disinfection ..........................................................................................................................................35
Preparing the Manual Wipe/Spra Disinfection .......................................................................................................37
Predisinfection .....................................................................................................................................................37
Cleaning ..............................................................................................................................................................37
Final Disinfection..................................................................................................................................................38
Chapter 11 Maintenance ..........................................................................................................................................................39
Inspections and Checks ............................................................................................................................................39
Regular Checks....................................................................................................................................................39
Semi-annual Inspection ........................................................................................................................................39
Service Intervals .......................................................................................................................................................40
3-monthl service.................................................................................................................................................40
Annual Service .....................................................................................................................................................40
Replacing the Door Seal............................................................................................................................................41
Replacing the Power Cord .........................................................................................................................................41
Returns for Repair ....................................................................................................................................................41
Chapter 12 Disposal .................................................................................................................................................................43
Overview of Materials Used .......................................................................................................................................43
Chapter 13 Error Codes ............................................................................................................................................................45
Chapter 14 Technical Data .......................................................................................................................................................4
Chapter 15 Accessories............................................................................................................................................................51
Chapter 16 Device Log..............................................................................................................................................................53

Fisherbrand part-number 1
List of Figures
Figure 3-1 Incubator dimensions and required clearances................................................................................................ 11
Figure 3-2 Lift Points ..................................................................................................................................................... 12
Figure 4-1. Fisherbrand Basic 60L/100L/180L Gravit Incubator Front View...................................................................... 15
Figure 4-2. Fisherbrand Basic 60L/100L/180L Gravit Incubator Rear View ...................................................................... 16
Figure 4-3. Sensor S stem ............................................................................................................................................. 17
Figure 4-4. Basic Gravit Incubator Shelf S stem ............................................................................................................. 19
Figure 5-1. Sliding the Retaining Spring into the Support Rail ........................................................................................... 21
Figure 5-2. Support Rail Installation ................................................................................................................................ 22
Figure 5-3. Shelf Support Installation .............................................................................................................................. 23
Figure 5-4. Installing the Perforated Shelves.................................................................................................................... 23
Figure 7-1. Control Panel for Fisherbrand Basic Gravit Incubator ..................................................................................... 29
Figure 10-1. Door seal ................................................................................................................................................... 41

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part-number Fisherbrand

Fisherbrand 50152755 | 1
Safety Notes
Basic Operating Precautions
These operating instructions describe Fisherbrand incubators.
Fisherbrand incubators have been manufactured to the latest state of the art and have been
tested thoroughl for flawless functioning prior to shipping. However, the incubator ma present
potential hazards, particularl if it is operated b inadequatel trained personnel or if it is not used
in accordance with the intended purpose. Therefore, the following must be observed for the sake
of accident prevention:
 Never step into the unit.
 Fisherbrand incubators must be operated b adequatel trained and authorized professional
personnel.
 Fisherbrand incubators must not be operated unless these operating instructions have been
full read and understood.
 The present operating instructions, applicable safet data sheets, plant h giene guidelines
and the corresponding technical rules issued b the operator shall be used to create written
procedures targeted at personnel working with the subject matter device, detailing:
 the decontamination measures to be emplo ed for the incubator and the accessories
used with it,
 the safet precautions to be taken when processing specific agents,
 the measures to be taken in case of accidents.
 Repair work on the incubator must be carried out onl b trained and authorized expert personnel.
 The contents of these operating instructions are subject to change at an time without further
notice.
 Keep these operating instructions close to the incubator so that safet instructions and
important information are alwa s accessible.
 Keep the door open-times during operation alwa s as short as possible. The longer the door
is open, the higher can be a possible overshooting of the temperature. After a door opening
allow the incubator to the set-point temperature, before opening again.
 Should ou encounter problems that are not detailed adequatel in these operating instructions,
please contact Fisher Scientific International, LLC immediatel for our own safet .

| Safet Notes
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Chapter 1
Operational Safety Rules
The following rules must be heeded when working with Fisherbrand incubators:
 Observe the sample weight limits specified for our Fisherbrand incubator as a whole and its
shelving in particular; see “Technical Data” on page 47.
 Do not load the bottom of the interior workspace to avoid the risk of overheating an samples
placed there and to prevent the temperature sensor from being damaged.
 Arrange the samples evenl throughout the work space, making sure not to place them too
closel to the interior walls to ensure a uniform temperature distribution.
 Do not load our Fisherbrand incubator with substances that exceed the capabilities of the
available lab apparatus and Personal Protection Equipment to provide sufficient degrees of
protection to users and third parties.
 Check the door seal ever six months for proper sealing performance and possible damage.
 Do not process an samples containing hazardous chemical substances that ma be
released into the ambient air through defective seals or ma cause corrosion or other defects
on parts of the Fisherbrand incubator.
Warranty
Fisher Scientific International, LLC warrants the operational safet and functions of the
Fisherbrand incubators onl under the condition that:
 the incubator is operated and serviced exclusivel in accordance with its intended purpose
and as described in these operating instructions,
 the incubator is not modified,
 onl original spare parts and accessories that have been approved b Fisher Scientific care
used (third-part spares without Fisher Scientific International, LLC approval void the limited
warrant ),
 inspections and maintenance are performed at the specified intervals,
 an operation verification test is performed after each repair activit .
The warrant is valid from the date of deliver of the incubator to the customer.

Fisherbrand 50152755 | 3
Safet Notes | Chapter 1
Explanation of Safety Information and Symbols
Safety Notes and Symbols Used T roug out T ese
Operating Instructions
Additional Symbols for Safety Information
Indicates a hazardous situation which, if not avoided, will result in death or serious
injuries.
Indicates a hazardous situation which, if not avoided, could result in death or serious
injuries.
Indicates a situation which, if not avoided, could result in damage to
equipment or property.
NOTE
Is used for useful hints and information regarding the application.
Wear safet gloves!
Wear safet goggles!
Harmful liquids!

| Safet Notes
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Chapter 1
Symbols on t e Incubator
Electric shock!
Hot surfaces!
Fire hazard!
Explosion hazard!
Suffocation hazard!
Danger of tipping !
Observe operating instructions
Mark of conformit USA/Canada

Fisherbrand 50152755 | 5
Safet Notes | Chapter 1
Intended Purpose of t e Incubator
Correct Use
Fisherbrand incubators are laborator devices for preparing and cultivating cell and tissue
cultures. The devices emplo precision temperature control for simulating the specific
ph siological ambient conditions for these cultures.
Fisherbrand incubators are for professional use onl .
Incorrect Use
To avoid the risk of explosion do not load the incubator with tissue, material, or liquids that:
 are easil flammable or explosive,
 release vapor or dust that forms combustible or explosive mixtures when exposed to air,
 release poisons,
 do not pour an liquids on the bottom of the interior surface or into a collecting basin inside
the unit.
 release dust
 exhibit exothermic reactions
 are p rotechnical substances
 refrain also from pouring an liquids onto the internal base plate or inserting bowls filled with
liquids into the sample compartment.
Standards and Directives
The incubator complies with the following standards and guidelines:
 IEC/CAN/CSA-C22.2/UL 61010 - 1
 IEC/CAN/CSA-C22.2 61010 - 2 - 010
 China EEP Hazardous Substances Information
http://www.thermofisher.com/us/en/home/technical-resources/rohs-certificates.html.
Additionall , the incubator is in compliance with man other international standards, regulations
and directives not listed here. Should ou have an questions regarding compliance with national
standards, regulations and directives applicable for our countr , please contact our Fisherbrand
sales organization.

| Safet Notes
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| 50152755 Fisherbrand
Chapter 1

Fisherbrand 50152755 | 7
Delivery of the Incubator
Packaging
Fisherbrand incubators are delivered in a rugged packaging box. All packaging materials can be
separated and are reusable:
Packaging materials
Packaging carton: Rec cled paper
Foam elements: St rofoam (CFC-free)
Pallet: Chemicall untreated wood
Packaging film: Pol eth lene
Packaging ribbons: Pol prop lene
Acceptance Inspection
After the incubator has been delivered, check the deliver immediatel for:
 completeness,
 possible damage.
If components are missing or damage is found on the incubator or the packaging, in particular
damage caused b humidit and/or water, please notif the carrier as well as Fisherbrand
Technical Support immediatel .
Risk of injury
Should sharp edges have formed in damaged areas or elsewhere on the device, take
all necessary precautions to protect personnel handling the incubator. For example,
have them wear protective gloves and other personal protection equipment.

| Deliver of the Incubator
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| 50152755 Fisherbrand
Chapter 2
Scope of Supply
Incubators
Quantity of components supplied (pieces) Gravity Convection
Incubators
Perforated shelves 2
Support rail for shelf table-top incubators 4
Shelf support 4
Power cord 1
Clip springs for table-top incubators 4
Plug 1
Anti-tilt anchor 1
Operating manual 1
Summarized Safet Instructions 1

Fisherbrand 50152755 | 9
Installation
Ambient Conditions
Location Requirements
The incubator must onl be operated in a location that meets all of the ambient condition
requirements listed below:
 Installation location indoors in dr areas free from drafts.
 The dust burden ma not exceed the contamination categor 2 based on IEC61010-1. Using
the incubator in an atmosphere with electricall conductive dust is prohibited.
 The minimal distance to adjacent surfaces must be observed on all sides (see Section
“Incubator Dimensions and Clearances” on page 11)
 The operating room must be equipped with appropriate ventilation.
 Solid, level, fire-proof surface; no flammable materials opposite to the rear panel of the
incubator.
 Vibration-proof substructure (floor stand, lab table) capable of bearing the dead weight of the
incubator and its accessories (particularl if two devices are stacked).
 The electrical circuitr of the incubator has been designed for an operating height of up to
2000 m above sea level.
 Relative humidit up to 80% (maximum; preferabl 60-70%), non condensing.
 Should condensation exist, wait until the moisture has evaporated completel before
connecting the incubator to a power source and powering up.
 The ambient temperature must be within a range of +18 °C to +32 °C (64.4 °F to 89.6 °F).
 Avoid direct exposure to sunlight.
 Devices that produce excessive amounts of heat must not be placed near the incubator.
 Place the incubator on a floor stand (option; to be ordered separatel ), never on the lab floor.
This prevents the penetration of dust or dirt into the device.
 Power line voltage variations must not exceed ±10 % of the nominal voltage.
 Transient surges must lie within the range of levels that normall occur in the power suppl
s stem. The impulse withstand voltage based on surge categor II of IEC 60364-4-443 shall
be applied at the nominal voltage level.

| Installation
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Chapter 3
 Consider installing one dedicated upstream circuit breaker per incubator to avoid multiple
device failures in case of an electrical fault.
Intermediate Storage
When the incubator is placed in intermediate storage, make sure that the ambient temperature is
between 20 °C to 60 °C (68 °F to 140 °F) and the maximum relative humidit does not exceed
90%, non-condensing.
Room Ventilation
Heat dissipating from the incubator during continuous operation ma cause a change in the room
climate.
 Therefore, the incubator must onl be installed in rooms with sufficient ventilation.
 Do not install the incubator in room recesses without ventilation.
 When several devices are to be placed in the same room, additional ventilation ma have to
be provided as necessar .
 To avoid an impact of the heat dissipated b the incubator on the ambient climate the room
must be vented b means of a laborator -grade ventilation s stem that complies with
applicable local and national health and safet regulations and has sufficient capacit .
 If excessive temperatures tend to occur in the operating room, be sure to provide a thermal
protection means that cuts out the power suppl to mitigate the impact of over-temperature
scenarios.
Contamination hazard
Do not place the incubator directly on the lab floor, but mount it on the floor stand or on
a lab work surface (option; to be ordered separately). Contaminants, such as bacteria,
viruses, fungi, prions, and other biological substances may use the open door to
migrate easily from the floor into the incubator’s work space.

Fisherbrand 50152755 | 11
Installation | Chapter 3
Incubator Dimensions and Clearances
Figure 3-1 Incubator dimensions and re uired clearances
Table 3-1 Incubator Dimensions
Type A (mm/inch
*
) B (mm/inch) C (mm/inch D (mm/inch)
Fisherbrand Basic 60L Gravit
Incubator 120 V
530 / 20.8 565 / 25.2 720 / 28.3 540 / 21.3
Fisherbrand Basic100L Gravit
Incubator 120 V
640 / 25.2 565 / 25.2 820 / 32.3 650 / 25.6
Fisherbrand Basic180L Gravit
Incubator 120 V
640 / 25.2 738/ 29.1 920 / 36.2 650 / 25.6
Fisherbrand 75L Incubator 230 V 530 / 20.8 565 / 25.2 720 / 28.3 540 / 21.3
Fisherbrand 117L Incubator 230 V 640 / 25.2 565 / 25.2 820 / 32.3 650 / 25.6
Fisherbrand 194L Incubator 230 V 640 / 25.2 738/ 29.1 920 / 36.2 650 / 25.6
*
Dimensions in inches are rounded equivalents specified for information onl . Depth of handle /displa (66 mm/2.6 in) not included in overall depth specified;
height of adjustable feet (36 mm/1.4 in) not included in overall height specified.
Table 3-2 Required Clearances
E (mm/inch) F (mm/inch) G (mm/inch) H (mm/inch)
80 / 3.2 50 / 2 300 / 12 300 / 12

| Installation
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| 50152755 Fisherbrand
Chapter 3
Transport
Table-top incubators
For transport, do not lift the incubator using the doors or components attached to the incubator as
lift points.
Figure 3-2 Lift Points
Heavy loads! Lift with care!
To avoid injury through physical strain, such as strain traumata and slipped discs, do
not attempt to lift the incubator alone!
To avoid injury through dropped loads, be sure to wear Personal Protection Equipment,
such as safety shoes, when lifting the incubator.
To avoid crushing your fingers or hands (particularly in a closing door) or damaging the
incubator, do not use any other lift points than those indicated in the illustration above.

Fisherbrand 50152755 | 13
Installation | Chapter 3
Installing t e Anti-tilt Anc or
Table-top incubators
The anti-tilt anchor secures the device to a solid part of a building. The anti-tilt anchor is to be
mounted on the side opposite of the door hinges.
Bend the fixing tabs of the anti-tilt anchor up on one side and down on the other b an angle of
approx. 90°.
1. Do not use this position if the door is hinged on this side. Right-hand hinges represent the
standard configuration.
2. Preferred position.
3. Alternative position. Do not use if the door is hinged on this side.
4. Remove the bracket screws. Use the preferred position, if possible.
5. Fix the anti-tilt anchor with the bracket side down to the unit.
6. Position the unit with the anti-tilt anchor to in an angle of approx. 90° +/- 20%.
7. Take care that the stacking feet of the unit are still in correct place on the lower unit or on the
stacking adapter.
8. Fix the anti-tilt anchor to a solid part of the building.
Additionall , the following caution notes must be heeded at all times:
Unsafe part of the building!
Install the anti-tilt anchor to a solid part of the building, which is able for shoring loads.
The installation has to be carried out by qualified personnel only.
The connection to the building must be carried out with appropriate screws and
dowels according to the consistence of the building part.

| Installation
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| 50152755 Fisherbrand
Chapter 3
Risk of overheating with stacked devices
To avoid the risk of electrical components and the outer enclosure overheating or
temperature control failing due to insufficient ventilation, do not exceed the specified
stacking height!
Risk of tipping and dropping of stacked devices
You should be aware at all times that stacked devices do not form a stable unit, even
when the stacking pads and frames are correctly used. The top device may tip over
and drop down when being transported in a stack. To avoid injury to persons and
damage to equipment, do not attempt to move stacked devices as a unit! Separate and
move each device one by one, then restack them.