GE HEALTHCARE Monitor UV-900 User manual

Monitor UV-900
Operating Instructions
Original instructions

Table of Contents
41 Introduction ..........................................................................................................
41.1 About this manual ................................................................................................................................
51.2 Important user information .............................................................................................................
61.3 Regulatory information ......................................................................................................................
71.3.1 EU Directives ....................................................................................................................................
81.3.2 Eurasian Customs Union ............................................................................................................
91.3.3 Regulations for USA and Canada ...........................................................................................
91.3.4 Other regulations and standards ............................................................................................
101.4 User documentation ...........................................................................................................................
122 Safety instructions ...............................................................................................
122.1 Safety precautions ...............................................................................................................................
172.2 Labels .........................................................................................................................................................
192.3 Emergency procedures ......................................................................................................................
202.4 Procedures for decommissioning .................................................................................................
212.5 Declaration of Hazardous Substances (DoHS) ........................................................................
243 System description ..............................................................................................
243.1 Illustrations ..............................................................................................................................................
253.2 Flow cell .....................................................................................................................................................
273.3 Monitor principle ...................................................................................................................................
304 Installation ............................................................................................................
314.1 Unpacking ................................................................................................................................................
314.2 Site requirements ..................................................................................................................................
314.3 Transport ..................................................................................................................................................
314.4 Assembly ..................................................................................................................................................
374.5 Connecting electrical cables ...........................................................................................................
415 Operation ..............................................................................................................
425.1 Starting the instrument ......................................................................................................................
425.2 Reading absorbance values ............................................................................................................
435.3 Menu selection and settings ...........................................................................................................
505.4 Changing flow cell ................................................................................................................................
515.5 UV cell calibration .................................................................................................................................
545.6 Restart after power failure ...............................................................................................................
556 Maintenance .........................................................................................................
566.1 Cleaning before planned service ...................................................................................................
576.2 Cleaning the instrument housing ..................................................................................................
576.3 User maintenance schedule ...........................................................................................................
576.4 Cleaning-in-place .................................................................................................................................
586.5 Checking the instrument ...................................................................................................................
596.6 Cleaning the flow cell and optical connectors ........................................................................
2 Monitor UV-900 Operating Instructions 28962214 AD
Table of Contents

606.7 Storage ......................................................................................................................................................
627 Troubleshooting ...................................................................................................
637.1 General ......................................................................................................................................................
637.2 Faults and actions ................................................................................................................................
647.3 Error messages ......................................................................................................................................
668 Reference information ........................................................................................
668.1 Technical specifications .....................................................................................................................
698.2 Health and Safety Declaration Form ...........................................................................................
708.3 Menu overview .......................................................................................................................................
718.4 Accessories and spare parts ...........................................................................................................
738.5 Ordering information ..........................................................................................................................
Monitor UV-900 Operating Instructions 28962214 AD 3
Table of Contents

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regula-
tory information, intended use of the Monitor UV-900 system, and lists of associated
documentation.
In this chapter
See pageSection
41.1 About this manual
51.2 Important user information
61.3 Regulatory information
101.4 User documentation
1.1 About this manual
Purpose of the Operating
Instructions
The Operating Instructions provides you with the instructions needed to handle Monitor
UV-900 in a safe way.
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu.
Hardware items are identified in the text by bold text (for example, Power).
4 Monitor UV-900 Operating Instructions 28962214 AD
1 Introduction

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, operating or
maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Intended use
Monitor UV-900 is a UV-absorbtion monitor for use in liquid chromatograhy.
Monitor UV-900 is intended for research use only, and shall not be used in any clinical
procedures, or for diagnostic purposes.
Prerequisites
In order to operate Monitor UV-900 in the way it is intended:
•The user must have a general understanding of bioprocessing equipment and with
the handling of biological materials.
•The user should understand the concepts of liquid chromatography.
•The user must read and understand the Safety Instructions chapter in the Operating
Instructions.
•Monitor UV-900 must be installed in accordance with the site requirements and in-
structions in the Operating Instructions.
Monitor UV-900 Operating Instructions 28962214 AD 5
1 Introduction
1.2 Important user information

Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to pro-
ceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Note:
A tip contains useful information that can improve or optimize your procedures.
Tip:
1.3 Regulatory information
Introduction
This section lists the regulations and standards that apply to the Monitor UV-900.
6 Monitor UV-900 Operating Instructions 28962214 AD
1 Introduction
1.2 Important user information

Manufacturing information
The table below summarizes the required manufacturing information.
InformationRequirement
GE Healthcare Bio-Sciences AB,Name and address of manufacturer
Björkgatan 30, SE 751 84 Uppsala, Sweden
In this section
See pageSection
71.3.1 EU Directives
81.3.2 Eurasian Customs Union
91.3.3 Regulations for USA and Canada
91.3.4 Other regulations and standards
1.3.1 EU Directives
Conformity with EU Directives
This product fulfills the European Directives listed below. See the EU Declaration of
Conformity for the directives and regulations that apply for the CE marking.
If not included with the product, a copy of the EU Declaration of Conformity is available
on request.
TitleDirective
Machinery Directive (MD)2006/42/EC
Electromagnetic Compatibility (EMC) Directive2014/30/EU
Low Voltage Directive (LVD)2014/35/EU
Restriction of Hazardous Substances (RoHS) Directive2011/65/EU
Monitor UV-900 Operating Instructions 28962214 AD 7
1 Introduction
1.3 Regulatory information
1.3.1 EU Directives

CE marking
The CE marking and the corresponding EU Declaration of Conformity is valid for the in-
strument when it is:
•used according to the Operating Instructions or user manuals, and
•used in the same state as it was delivered from GE, except for alterations described
in the Operating Instructions or user manuals.
1.3.2 Eurasian Customs Union
Introduction
This section contains additional regulatory information to comply with the Eurasian
Customs Union technical regulations.
Manufacturer and importer
information
The table below summarizes the manufacturer and importer information required by
the Eurasian Customs Union.
InformationRequirement
See Manufacturing informationName and address of manufacturer
Telephone: + 46 771 400 600Telephone number of manufacturer
GE Healthcare LLCImporter and/or company for obtain-
ing information about importer GE Healthcare Life Sciences
Presnenskaya nab., 10C, 12th floor
RU-123 317 Moscow, Russian Federation
Telephone 1: + 7 495 411 9714
Fax nr: + 7 495 739 6932
Email: [email protected]
8 Monitor UV-900 Operating Instructions 28962214 AD
1 Introduction
1.3 Regulatory information
1.3.1 EU Directives

1.3.3 Regulations for USA and Canada
FCC compliance
This device complies with part 15 of the FCC Rules. Operation is subject to the following
two conditions: (1) This device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause undesired
operation.
The user is cautioned that any changes or modifications not expressly approved
by GE could void the user’s authority to operate the equipment.
Note:
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide rea-
sonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequen-
cy energy and, if not installed and used in accordance with the instruction manual, may
cause harmful interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference in which case the user will be re-
quired to correct the interference at his own expense.
CAN ICES/NMB compliance
This product complies with the Canadian standard ICES-001/NMB-001 concerning
electromagnetic compatibility.
1.3.4 Other regulations and standards
Introduction
This section describes the standards that apply to the Monitor UV-900 system.
Environmental conformity
This product conforms to the following environmental requirements.
TitleRequirement
Waste Electrical and Electronic Equipment (WEEE) Directive2012/19/EU
Management Methods for the Restriction of the Use of Haz-
ardous Substances in Electrical and Electronic Products.
China RoHS
Monitor UV-900 Operating Instructions 28962214 AD 9
1 Introduction
1.3 Regulatory information
1.3.3 Regulations for USA and Canada

Standards, machinery and
electrical equipment
Standard requirements fulfilled by this product are summarized in the table below.
DescriptionStandard
Safety of machinery. General principles for design. Risk
assessment and risk reduction.
EN ISO 12100
Safety requirements for electrical equipment for mea-
surement, control, and laboratory use - Part 1: General
requirements.
EN 61010-1, IEC 61010-1,
UL 61010-1, CAN/CSA-
C22.2 No. 61010-1
Electrical Equipment for Measurement, Control, and
Laboratory Use-EMC requirements-Part 1: General re-
quirements
EN 61326-1
(Emission according to CISPR 11, Group 1, class A)
NOTICE
This equipment is not intended for
use in residential environments
and may not provide adequate
protection to radio reception in
such environments.
Industrial, Scientific and Medical (ISM) Radio Frequency
Generators (Canada)
ICES-001/NMB-001
1.4 User documentation
In addition to these Operating Instructions, the documentation package supplied with
Monitor UV-900 also includes product documentation binders containing detailed
specifications and traceability documents.
The most important documents in the document package with regard to technical aspects
of Monitor UV-900 are listed below.
10 Monitor UV-900 Operating Instructions 28962214 AD
1 Introduction
1.3 Regulatory information
1.3.4 Other regulations and standards

System-specific documentation
ContentUser documentation
System description. User instructions, method
creation, operation, maintenance and trou-
bleshooting.
Monitor UV-900 Operating
Instructions
Detailed system description. Comprehensive user
instructions, method creation, operation, ad-
vanced maintenance and troubleshooting.
Monitor UV-900 User Manual
Document whereby the manufacturer ensures
that the product satisfies and is in conformity with
the essential requirements of the applicable direc-
tives.
EU Declaration of Conformity for
Monitor UV-900
Monitor UV-900 Operating Instructions 28962214 AD 11
1 Introduction
1.4 User documentation

2 Safety instructions
About this chapter
This chapter describes safety compliance, safety labels, general safety precautions,
emergency procedures, power failure and recycling of Monitor UV-900.
In this chapter
This chapter contains the following sections:
See pageSection
122.1 Safety precautions
172.2 Labels
192.3 Emergency procedures
202.4 Procedures for decommissioning
212.5 Declaration of Hazardous Substances (DoHS)
2.1 Safety precautions
Introduction
The Monitor UV-900 is powered by mains voltage and handles pressurized liquids that
may be hazardous. Before installing, operating or maintaining the monitor, you must be
aware of the hazards described in this manual. Follow the instructions provided to avoid
personal injury or damage to the equipment. The safety precautions in this section are
grouped into the following categories:
•General precautions
•Using flammable liquids
•Personal protection
•Installing and moving the instrument
•System operation
12 Monitor UV-900 Operating Instructions 28962214 AD
2 Safety instructions

•Maintenance
General precautions
Always follow these General precautions to avoid injury when using the Monitor UV-900
instrument.
WARNING
Do not operate Monitor UV-900 in any other way than described
in the Monitor UV-900 manual.
WARNING
Only properly trained personnel may operate and maintain the
product.
WARNING
Do not use any accessories not supplied or recommended by GE.
WARNING
Do not use Monitor UV-900 if it is not working properly, or if it has
suffered any damage, for example:
•damage to the power cord or its plug
•damage caused by dropping the equipment
•damage caused by splashing liquid onto it
Monitor UV-900 Operating Instructions 28962214 AD 13
2 Safety instructions
2.1 Safety precautions

Using flammable liquids
When using flammable liquids with the Monitor UV-900, follow these precautions to
avoid any risk of fire or explosion.
WARNING
Fire Hazard. Before starting the system, make sure that there is
no leakage.
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE) during
operation and maintenance of this product.
WARNING
Hazardous substances and biological agents. When using haz-
ardous chemical and biological agents, take all suitable protective
measures, such as wearing protective clothing, glasses and gloves
resistant to the substances used. Follow local and/or national
regulations for safe operation and maintenance of Monitor UV-900.
WARNING
Spread of biological agents. The operator must take all necessary
actions to avoid spreading hazardous biological agents. The facility
must comply with the national code of practice for biosafety.
14 Monitor UV-900 Operating Instructions 28962214 AD
2 Safety instructions
2.1 Safety precautions

Installing and moving the
instrument
WARNING
Supply voltage. Before connecting the power cord, make sure that
the supply voltage at the wall outlet corresponds to the marking
on the instrument.
WARNING
Protective ground. The product must always be connected to a
grounded power outlet.
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by GE.
WARNING
Access to power switch and power cord with plug. Do not block
access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.
WARNING
High intensity UV light. This product uses high intensity ultra-violet
light. Do not disconnect the optical fibers while the lamp is on.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
Monitor UV-900 Operating Instructions 28962214 AD 15
2 Safety instructions
2.1 Safety precautions

System operation
WARNING
If liquid is spilled on the equipment, the electrical power supply
must be disconnected immediately. The equipment must be com-
pletely dry on the inside and the outside before reconnecting the
power supply.
WARNING
Hazardous biological agents during run. When using hazardous
biological agents, run System CIP and Column CIP to flush the
entire pump with bacteriostatic solution (e.g. 1M NaOH) followed
by a neutral buffer and finally distilled water, before service and
maintenance.
WARNING
Hazardous chemicals during run. When using hazardous chemi-
cals, run System CIP and Column CIP to flush the entire system
tubing with distilled water, before service and maintenance.
Maintenance
WARNING
Electrical shock hazard. All repairs should be done by service
personnel authorized by GE. Do not open any covers or replace
parts unless specifically stated in the user documentation.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by GE may be used for maintaining or
servicing the product.
16 Monitor UV-900 Operating Instructions 28962214 AD
2 Safety instructions
2.1 Safety precautions

WARNING
Corrosive substance. NaOH is corrosive and therefore dangerous
to health. When using hazardous chemicals, avoid spillage and
wear protective glasses and other suitable Personal Protective
Equipment (PPE).
WARNING
When using hazardous chemicals, make sure that the entire flow
cell has been flushed thoroughly with bacteriostatic solution, for
example NaOH, and distilled water, before service and mainte-
nance.
WARNING
When using hazardous chemicals, make sure that the entire system
has been flushed thoroughly with bacteriostatic solution, e.g. NaOH,
and distilled water before service and maintenance.
WARNING
Disconnect power. Always disconnect power from the instrument
before replacing any component on the instrument, unless stated
otherwise in the user documentation.
2.2 Labels
Introduction
This section describes the system label on Monitor UV-900 and its meaning.
Monitor UV-900 Operating Instructions 28962214 AD 17
2 Safety instructions
2.1 Safety precautions

System label
The illustration below shows the system label.
The specific data shown on the system label below is only an example. Actual
data is specific for each individual system and may vary from system to system.
Note:
The system label information is explained in the table.
DescriptionLabel text
Code number.Code no:
Serial number.Serial no:
Year (YYYY) and month (MM) of manufacture.Mfg Year:
Voltage.Voltage:
Supply voltage frequency.Frequency:
Max power.Max power:
Warning! Read the user documentation before using
the system. Do not open any covers or replace parts
unless specifically stated in the user documentation.
This symbol indicates that waste electrical and electron-
ic equipment must not be disposed as unsorted munic-
ipal waste and must be collected separately. Please
contact an authorized representative of the manufac-
turer for information concerning the decommissioning
of equipment.
18 Monitor UV-900 Operating Instructions 28962214 AD
2 Safety instructions
2.2 Labels

DescriptionLabel text
This symbol indicates that the product contains haz-
ardous materials in excess of the limits established by
the Chinese standard GB/T 26572 Requirements of
concentration limits for certain hazardous substances
in electrical and electronic products.
The system complies with applicable European direc-
tives.
Eurasian Conformity mark: the single conformity mark
indicates that the product is approved for circulation
on the markets of the member states of the Eurasian
Customs Union.
The system complies with the requirements for
electromagnetic compliance (EMC) in Australia and New
Zealand.
CAN ICES-1/NMB-1 indicates that this product complies
with the Canadian standard ICES-001 concerning
technical requirements relative to radiated noise emis-
sions from Industrial, Scientific and Medical radio fre-
quency generators.
CAN ICES-1/NMB-1
This symbol indicates that Monitor UV-900 has been
certified by a Nationally Recognized Testing Laboratory
(NRTL). NRTL means an organization, which is recognized
by the US Occupational Safety and Health Administra-
tion (OSHA) as meeting the legal requirements of Title
29 of the Code of Federal Regulations (29 CFR), Part
1910.7.
2.3 Emergency procedures
Introduction
This section describes how to perform an emergency shutdown of Monitor UV-900. The
section also describes the result in the event of power failure.
Monitor UV-900 Operating Instructions 28962214 AD 19
2 Safety instructions
2.2 Labels

Emergency shutdown
In an emergency situation, do as follows to stop the run:
Switch off power to the instrument by switching the On/off switch on the rear panel to
the (O) position. If required, disconnect the mains power cord. The run is interrupted
immediately.
Power failure
In the event of power failure the run is interrupted immediately.
2.4 Procedures for decommissioning
Introduction
This section contains information about the decommissioning of Monitor UV-900.
Decontamination
The product must be decontaminated before decommissioning. All local regulations
must be followed with regard to scrapping of the equipment.
Disposal of the product
When taking the product out of service, the different materials must be separated and
recycled according to national and local environmental regulations.
Recycling of hazardous
substances
Monitor UV-900 contains hazardous substances. Detailed information is available from
your GE representative.
20 Monitor UV-900 Operating Instructions 28962214 AD
2 Safety instructions
2.3 Emergency procedures