GeneAll GTI032A User manual

GENTi Advanced Automatic
Extraction Equipment
GTI032A
Users’ Manual
GeneAll Biotechnology Co., Ltd.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
Caution :
GeneAll Biotechnology Co., Ltd. Reserves the right to modify this manual at
any time without notice.
This document contains proprietary information protected by copyright. All rights reserved.
Without prior written consent from GeneAll biotechnology Co., Ltd., any part of the manual shall
not be duplicated, reproduced or translated into any other languages.
Before the initial use of this instrument, please carefully read this manual!
Document Version: August 2020, Version 1.0

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
Important Notes
1. General Practice
Caution :
Because of the important information in this column, please read it carefully. Failure
to follow the advice in this column will possibly result in damage or malfunctioning
of the machine.
2. Safety
During the operation, maintenance, or repair of the machine, the following safety measures should
be taken. Otherwise, the guard provided by the machine is likely to be damaged, the rated safety
level to be reduced, and the rated operation conditions to be affected.
GeneAll Biotechnology Co., Ltd. Shall not be in any way responsible for the consequences resulted
from buyer’s not observing the following requirements.
a) Grounding Considerations
A.C. power’s grounding should be reliable for fear of an electric shock. The 3-pin plug with the
machine’s power line is a safety device that should be matched with a grounded socket. Never let
the third ground pin floating. If the 3-pin plug cannot be inserted, it is recommended to ask an
electrician to install an appropriate power socket.
b) Keep Away from Electric Circuits
The operator is not allowed to open the machine. Changing components or adjusting certain
parameters inside the device must only be accomplished by the certificated professional
maintenance personnel. Do not change elements while the power is still on.
c) A.C. Power Considerations
Before turn on the power, always check and insure that the mains voltage is within the required A.C.
100-240V and insure that the current rating load of the power socket meet the required specification.
d) A.C. Power Line Considerations
As an accessory of the machine, the A.C. power line should be a default one. If it is damaged, the
A.C.
power line can not be repaired, but must be replaced with a new one. The power line should be
free of heavy objects during the machine’s operation. Keep the power line away from the place
where people ambulate frequently.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
e) Connect the A.C. Power Line
While connect or disconnect the power line, you should hold the 3-pin plug with your hand. Insert
the plug thoroughly into the socket to ensure good contact between the plug and socket. Pull the
plug, not the line, when you need to disconnect to the mains.
f) Design Environments
The machine should be placed in a low-humidity, dust-free, and good-ventilation room without
caustic gas or powerful magnetic interference. In addition, water sources, such as pools and water
pipes, should be separated a distance from the machine.
Never cover or obstruct the openings of the machine, which are designed for ventilation and to
prevent the device’s interior from being too hot. When a single device is running, the shortest
distance between its openings and the nearest object is 25cm; otherwise, when two devices or
above are running at the same time, the shortest distance is 40 cm. Do not place the device on a
soft surface, because that will
result in adverse ventilation near the device’s bottom openings.
Too high temperature will lead to degraded performance or failure of the machine. Therefore, the
device should be protected against any kind of heat sources like sunlight, ovens, or central heating
equipment.
If the machine is set aside for a long time, it is recommended to disconnect the power line to mains
and cover the device with a piece of soft cloth or plastic to prevent dust from entering.
Caution :
Once one of the following events occurs, you are suggested to disconnect the power
line with mains, and contact the distributor or ask a certificated maintenance worker
for help.
Liquid into the device;
The device sprinkled or drenched;
The device malfunctioning, giving off abnormal sound or door;
The device falling onto the floor or its shell damaged;
Significant changes in the device’s performance.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
3. WARRANTY AND SERVICE INFORMATION
a) Warranty
GeneAll Biotechnology Co., Ltd. is responsible for the replacement for failures caused by material
and manufacturing defects within 1 month after goods delivery.
GeneAll Biotechnology Co., Ltd. is responsible for maintenance of failures caused by material and
manufacturing defects within 12 months after goods delivery. GeneAll Biotechnology Co., Ltd. is
responsible for selectively repairing and replacing instruments with confirmed defects within
warranty period.
Maintained products shall be sent to maintenance department designated by GeneAll Biotechnology
Co., Ltd. by the user. The freight incurred thereof shall be borne by the user. GeneAll Biotechnology
Co., Ltd. will bear the freight incurred by returning instrument. (For domestic users only) For
maintenance out of warranty period, GeneAll Biotechnology Co., Ltd. will charge for certain
maintenance cost.
b) Warranty Terms
The above warranty is not applicable to defective devices with incorrect use, abnormal operating
conditions, improper application, and unauthorized maintenance or alteration.
GeneAll Biotechnology Co., Ltd. makes no express warranties other than those which are described
herein. Any descriptions in sales promotion under specific conditions shall not create an express
warranty that the goods shall conform to such description.
Caution :
After unpacking, immediately check the goods against the packing list. In case of
damaged or missed goods, please immediately contact the supplier.
After qualification of acceptance, properly fill in the product acceptance sheet and
send (or fax) the copied sheet to the supplier for filing and maintenance.
After unpacking, the packing box and packing materials shall be well kept for future
repair. For the damage of instrument occurring during transporting to the repair
department due to improper package, GeneAll Biotechnology Co., Ltd. shall bear no
liabilities.
All upgrades of the equipment and software shall be operated by the engineer of
the manufacturer. GeneAll company shall not be liable for any equipment failure
caused by unauthorized updates and modifications.
After-sale Service Hotline: [phone]

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
Contents
Chapter I Introduction
1. Instrument Features
2. APPLICATIONS
Chapter II Features
1. Normal Working Conditions
2. Transportation and Storage Conditions
3. Basic Parameters
4. Basic Performance
5. Software Functions
Chapter III Preparations
1. Structure Diagram
2. Check before Startup
3. Placement of 96 deep well plate
4. Startup
Chapter IV Operation Guide
1. Upper computer operating interface
Chapter V Fault Analysis and Troubleshooting

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
Chapter I Introduction
This chapter mainly introduces the features and applications of the instrument.
1. Instrument Features
This product adopts a magnetic bead extraction technology to extract and purify nucleic acid
through precision transmission and controlling step motor by single chip microcomputer. It has
features as follows: small volume, light weight, low noise, full-closed workaround; Embedded real-
time operating system; Transparent working chamber facilitating the observation of working
conditions; Large LCD panel, easy to operate. It has powerful functions such like door open
protection, ultra-limit position protection and alarm. It is safer and more reliable for use. It can be
used to extract DNA, RNA from whole blood, cells, and tissues and so on, so as to save time and
labor.
2. Instrument Applications
This product can be used for separation and purification of nucleic acid in molecular biology
laboratory and clinical laboratory.
Chapter II Features
This chapter mainly introduces the usage, transportation and storage conditions of the instrument,
as well as its basic parameters, performance and functions.
1. Normal Working Conditions
Ambient temperature: 10℃~ 30℃
Relative humidity: 20%-70% without condensation
Power supply: AC100-240V 50-60Hz
Caution :
Before power on, please confirm whether the working conditions meet the above
requirements. Pay special attention to the power line is reliably grounded.
2. Transportation and Storage Conditions
Ambient temperature: -20℃+ 55℃
Relative humidity: ≤80%
3. Basic Parameters

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
Model
Parameters
GTI032A
Sample size
32pcs/time
Magnetic rod (fixed)
4x8 pcs
Sample range
20 ~ 1000ul
Magnetic bead collection efficiency
>98%
Most suitable size of magnetic beads
0.2~1.0um
Overall dimensions mm
430*395*435 (L*W*H)
Net weight
32.5kg
4. Basic performance
Operating temperature: room temperature
Program storage: 250 files
5. Software functions
File edit, view, modify and delete functions
File run, pause, and stop functions
File import & export by U disk
Voice prompt function
Fault protection and alarm function
UV lamp on and timed off
Upgrade by U disk
Caution :
The above software features are for reference only. GeneAll reserves the right to
change the software without notice.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
Chapter III Preparations
This chapter mainly describes the structure, the functions of screen and keys on the screen, as well
as the preparations before the startup. Before the first operation of this instrument, please read this
chapter carefully and make sure you are familiar with the contents of this chapter.
1. Structure Diagram
Back view of full automatic nucleic acid extraction and purification system
①Case Body ②Display ③Operating room ④ Where 96 deep well plate is placed
⑤Closing cover ⑥Power switch ⑦ Standard power outlet ⑧USB interface
⑨Sticker ⑩RS232
Caution :
The fuse seat is fitted with a fuse for which the requirement is: F 250V 6A Ø5x20.
Fuses must be replaced using products that meet the requirements. Contact the
supplier or manufacturer for fuses.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
2. Check before Startup
The following contents should be confirmed before power on:
2.1 Whether the power supply meets the required voltage of the instrument;(see chapter 2 of this
document for power requirements)
2.2 Confirm that the power cord plug has been reliably inserted into the power socket;
2.3 Reliable grounding of power cord;
2.4 Whether the 96 deep well plates is in place.
2.5 Placement of 96 deep well plate
2.5.1 There are four heating blocks placed on each plate with a limit block on each side to assist in
positioning, and a spring plate on each side to help fix the 96 deep well plate.
2.5.2 Note: Make sure the 96 deep well plate is placed evenly before starting up.
2.6 Startup
Switch on the automatic nucleic acid purification system, and the instrument will conduct self-test.
Self-test takes about 10 seconds. Please wait patiently. If no problem is found in the self-test, the
main menu will appear on the screen, and then the user can edit, review, modify and delete files.
Chapter IV Operation Guide
This chapter explains in detail how to edit/review/modify/delete files, and how to run/stop files. It
also introduces the meaning of protection interface in the process of file running.
Warning !
If there is any abnormal sound or display after the instrument is switched on, or
there is a fault alarm during the instrument self-testing, please switch off the power
immediately and contact GeneAll.
Ready? Let's get started!
1. Upper computer operating interface
a. Operation main interface
After the system is started, enter the main interface of nucleic acid purification operation, which
mainly has the following functions: Run, New File, Ultraviolet Lamp and System Setting. The interface
center displays the operation state of the instrument. If the instrument is in operation state, click
the icon of the center to further check the detailed operation information. The followings are the
idle and runtime main interfaces, respectively.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
The bottom four function buttons from left to right are: Run, New File, UV Lamp, and System Setting.
The middle operation status button and status information are displayed only when the equipment
is in running status. Press the Running button to enter the running state interface to view the
detailed information of the running state.
b. Run
Click run icon on the main interface to enter the file browsing interface. You will see buttons for
browsing menu selection on the left to select files, history or U disk files, information browsing in
the middle, function buttons in the bottom, and main interface icons in the upper left corner to
return to the main interface. Select different browsing menu items, the bottom function button and
browsing information will be different.
The following figure shows the file browsing interface.
A list of stored files is in the middle, showing up to 9 files per page and totally 250 files. The current
selected file is displayed in white. Click the current selected file to enter the file editing interface.The
page information and page forward/back button is in the right; the function button is in the bottom,
including Export, Delete, New, Edit, and Run.
Export: Copy the selected file to the default u-disk directory (under the \pcrex\ GeneAll of u-

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
disk directory).
Delete: Delete the selected file from the instrument.
New: After creating the default file, go to the file editing interface to edit the new file.
Edit: Go to the File Editing Interface to edit the current selected file.
Run: Run the selected file.
The following image shows the history browsing interface.
A list of 9 files that were last edited and run is in the middle. The current selected file is displayed
in white.
Click the current selected file to enter the file editing interface. T the function button is in the
bottom, including Clear Record, Edit, and Run.
Clear: Clear history Edit: Go to the file edit interface to edit the selected file Run: Run the selected
file
The following figure shows the U disk file browsing interface, which can only be displayed when
the U disk is inserted. If the U disk is not inserted, it prompts failure to detect the U disk.
In the center is the ".ebr "file list that conforms to the file name standard in the default directory

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
of U disk (under the \pcrex\ GeneAll of u-disk directory) (a legal filename consists of an English
letter, a number, and an underscore, and a filename that does not include a suffix is no longer than
12 characters long). The current selected file is displayed in white. The page information and page
forward/back button are in the right; the function button is in the bottom, including the import.
Import: Copy the selected file to the instrument
c. Running files
Click on Run button on the file browsing interface;
Click on Run button in the history browsing interface;
Click on Run button in the file save interface.
The lysis and elution temperatures are displayed on top of the running interface. The lower left side
of the interface shows the steps undergoing, including waiting time, mixing time, binding time, and
remaining time.
The bottom right side of the interface shows the number of columns being heated. At the bottom
is the function button, including: Stop, Pause, Pause/Reset.
Stop: Stop the experiment.
Pause: Suspend the experiment.
Pause/Reset: Suspend the experiment and Raise the magnetic rods.
d. UV light setting
In the operation main interface, click ultraviolet lamp setting to enter the ultraviolet lamp setting
interface.
After setting ultraviolet lamp turn-on time, click ok to turn to the ultraviolet lamp turn-on time set

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
state interface or click cancel button to return to the main interface.
e. System setting
Click on the system settings to enter the system settings interface in the operation main interface.
The 8 buttons in the middle correspond to 8 Settings entries. Click the button in the upper right
corner to return to the main interface.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
f. Date &Time
Click the Date & Time button in the system setting interface, enter the Date & Time setting interface,
and click ok or cancel button after setting time or date to return to the system setting interface.
g. Language selection
In the system settings interface click language into the language settings interface, select Chinese
or English, click OK or cancel button to return to the system settings interface.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
h. Backlight adjustment
Click backlight adjustment on the system setting interface to enter the backlight adjustment
interface.
Turn up/down screen brightness by clicking and dragging the scroll bar, or by clicking certain spot
in the horizontal column to modify the position of the indicator sign, and then click ok or cancel to
return to the system setting interface.
i. Motion parameters
The parameters in this interface are only used by experienced engineers. Please do not modify
the parameters without authorization.
j. Prompt tone setting
Click the prompt tone to enter the prompt tone setting interface on the main interface. After setting
the alarm prompt, the keyboard prompt and the closing prompt, click OK or Cancel button to return
to the system setting interface.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
k. Screen calibration
In the operation main interface, click the screen calibration to enter the screen calibration interface,
and click the center of the cross icon in the upper left corner and lower right corner, then return to
the system setting interface.
l. Step name management
Click name management to enter the step name management interface in the system setting
interface, and the interface displays all the steps currently, and you can create New, Delete and
Clear. Click exit button to return to the system setting interface.
New: Add a new step name tag for English and Numbers only and slip line for up to 12 characters,
preferably no more than 8 characters Delete: Delete the selected custom name label. The default
name is not removable Clear: Remove all custom name tags, leaving only the default name tags.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
m. System update
After inserting the upgrade USB drive into the main interface, click the system update to enter the
system update interface(if the upgrade USB drive is not inserted or there is no upgrade file in the
USB drive, it will be indicated that the upgrade file is not detected.) After selecting the program
that needs to be upgraded (according to the upgrade file in the USB flash disk, automatically list
the items available for upgrade), click the Upgrade button to start the firmware upgrade process,
and click the Cancel button to return to the main interface.
After the upgrade is completed, according to the screen prompt, shutdown the device, pull out the
upgrade USB drive, and restart the device.
Caution:
Please restart the device according to the screen prompt message, and it is forbidden to shut
down the device during the upgrade process.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
Chapter V Fault Analysis and Troubleshooting
This chapter mainly introduces the possible fault phenomena, causes analysis and troubleshooting
of this instrument.
Fault Analysis and Troubleshooting
Serial No.
Fault phenomenon
Cause
Solution
1
Display screen is not on when
the power switch is turned on.
Power on.
Check the power
and switch on.
Fuse was blown down.
Replace the fuse(F
250V 3A Ф5x20).
The switch is damaged.
Replace switch.
Others.
Contact the supplier
or manufacturer.
2
When power switch turned on,
buzzing alarm sounds and
system error is displayed. Please
contact the manufacturer.
Error occurs during
self-inspection process.
Contact the supplier
or manufacturer.
8
Abnormal screen display
Screen wires
disconnected.
Contact the supplier
or manufacturer.
Screen control chip is
damaged.
9
Screen buttons don't work.
Touch screen is
damaged
Contact the supplier
or manufacturer.
Caution :
users are not allowed to open the housing of the instrument for inspection by himself
during the warranty period. If any failures that require opening of the housing for
inspection, the supplier or manufacturer should be contacted timely.

Operation Manual for GENTi Advanced Automatic Extraction Equipment
GeneAll Biotechnology Co., Ltd.
GeneAll Biotechnology Co., Ltd.
Add :
GeneAll B/D, 303-7 Dongnam-ro, Songpa-gu, Seoul, 05729, Korea.
Tel :
82 –2 –407 –0096
Fax :
82 –2 –407 –0779
E-mail :
[email protected]
WWW.geneall.COM