GeneDireX BLook User manual

Fig.1. Emission spectrum for the BLooK LED transilluminator
BLooKLED transilluminator
Catalog number: BK001
Serial number: BK27888001
Description
BLooK is a remarkable blue light LED transilluminator for the detection of nucleic acids
or protein under non-UV conditions. The wavelength of the special blue LED lights is
470 nm (fig 1), hence no damage to your nucleic acids or protein. Also, since UV is not
used, there is no need for any special personal eye or skin protection. The blue LED
lights are arranged under the viewing area (200 × 120 mm). An amber filter, on hinges,
is lowered into position once your gel is mounted. The stained gel is now ready for
viewing. This instrument has a specially designed ergonomic 4° angle, so users can
easily sit on a chair to see the experiment results. BLooK is designed to view the gel
after running electrophoresis on the gel stained with the Novel Juice, Novel Green,
Novel Green Plus, Nimble Juice or Nimble Juice RTYPE. Further, it is perfectly designed
for OnePCRTM, OnePCRTM HiFi, OnePCRTM HotStar, OnePCRTM Plus, OneMARK B, and
OneMARK 100, which contains the fluorescent stain compatible with the blue light
wavelength. However, BLooK is not suitable for ethidium bromide.
Features
 Ergonomic fusion-Patented 4° ergonomic viewing angle ( “Golden Angle” )
 Optimized for use with the nucleic acid and protein fluorescent dyes.
 Blue light source good for 30,000 hours.
 No risk of UV damage for high quality work experience.
 Smart power-saving function - Automatic power shut-off option at 5 minutes.
 Gel-cutting knife - Cut out the target from the gel for further experiment.
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A B C D
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Unit dimensions (W × L × H)
Gel viewing dimensions (W × L × H)
Weight (g)
Input voltage
LED source
LED life (hours)
Emission maxima (nm)
Store temperature
Operating temperature
Auto shut-off (min)
Filter type :
295 × 215 × 42 mm (11.6 × 8.4 × 1.6 in)
200 × 120 mm (7.8 × 4.7 in)
1280
100∼240V, 50 / 60 Hz
Built-in blue light LED module
>30,000
470
25°C
25°C ∼+40°C
5
Amber filter (ideal for Novel Juice, Novel Green,
Novel Green Plus, OnePCRTM, OnePCRTM HiFi,
OnePCRTM HotStar, OnePCRTM Plus, OneMark B,
OneMARK 100, Nimble Juice, Nimble Juice RTYPE )
Specifications
Contents:
(A) Power Cord: Used to connect the BLooK port with the power socket.
(B) BLooK LED transilluminator
(C) Gel-Cutting Knife: Cut out the target from the gel for further experiment.
(D) Replacement Blade: Used as the new blade.

Amber filter
The upper panel is the amber
filter used to observe the gel
upon being lowered in position.
Dented holder
A long, dented holder, which is
located close to the operator,
is used to store the gel-cutting knife.
Power inlet
is used for connecting
the power cord.
Light source
The light source is located below and
projects upward from the bottom.
Please first lower the amber filter in
position when in use for preventing the
strong light from harming the eyes.
Ventilation holes
are located on the same side
and enable the heat, which is
generated when power is on,
to be displaced.
ON/OFF button
is used for switching
on and off the power.
BLooK LED transilluminator
Operating
1. Place the BLooK LED transilluminator on a level bench, with enough space around
the unit for allowing air circulation and preventing overheating.
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2. Connect the power cord and the socket.
3. Place the gel on the transparent glass support area.
Note: It is recommended that gloves be worn to prevent skin contact with gel and staining agents.
4. Close the amber lid above the gel.
5. Press the ON / OFF to ON mode.
6. Now the fluorescent samples can be viewed or cut.
Note: For cutting fluorescent samples out of the gel, please lift the lid to access the gel.
7. When finished turn the transilluminator off.
8. Clean the transilluminator filter and surface by wiping with a wet, not scratching cloth.

Problem
Low sensitivity
Automatic shut- off
High background,
suboptimal, or no image
Cause
Wavelength may not be right
Automatic power-off system
Low sample concentration
Solution
Check the excitation and emission
wavelengths of the fluorescence dye
Press the power button again to
re-turn on the light
Check the concentration of sample
Cat. No.
LD001-1000
LD002-0500
LD003-0500
MB203-0100
MB205-0100
MB206-0100
MB207-0100
DM101-0100
DM110-0100
NJ001-0010
NJ002-0500
Description
Novel Juice
Novel Green
Novel Green Plus
OnePCRTM
OnePCRTM HiFi
OnePCRTM HotStar
OnePCRTM Plus
OneMARK 100
OneMARK B
Nimble Juice
Nimble Juice RTYPE
Size
1 ml
500 μl
500 μl
100 Reactions
100 Reactions
100 Reactions
100 Reactions
600 μl
600 μl
10 ml (100x)
500 mL
Troubleshooting
Refer to the table below to troubleshoot problems that you may encounter when viewing
with the BLooK LED transilluminator
Related Ordering Information
Note: Please, read the installation instruction carefully before installing the BLooK LED transilluminator.
This instrument is intended for clinical and research laboratory use and it must be operated only by specialized
personnel aware of the potential risks associated with the chemical and biological agents normally used with this
unit. This instrument is meant for use only by specialized personnel that know the health risks associated with
blue light radiation and with reagents that are normally used with this instrument. It is designed to shield only the
person working in front of the system and to observe the gel fluorescence emission clearly.
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Maintenance and Repair
Cleaning and Maintenance
The transilluminator should be damply wiped off with a soft cotton cloth. The cloth
should not be too wet. Do not use aggressive cleaning detergents or solvents.
After use of the transilluminator, we recommend to wipe off the frame and the filter glass
with a damp cloth. Take care that the cloth is not soaked with scrubbing or corrosive
detergents as this could damage the filter glass. We suggest using alcohol, glass cleaners
or similar mild detergents.
Pay attention to wearing appropriate gloves when cleaning areas (as the filter plate of
the transilluminator, switches, protection shield) which may have been in contact with
carcinogenic or toxic reagents. The protection shield is made of tempered glass and
must only be cleaned with a damp cloth.
Method of Replacing with the New Blade
When replacing with the new blade, unscrew the black handling part of the knife
counterclockwise from the blue part. Push out the blue part’s blade-holding section.
Remove the old blade, and open the “SPARE BLADE” for taking out the new replacement
blade. Insert it into the X-shaped blade insertion hole. Restore the blue connector back
into the original position, and then screw tight the blue part with the black handling part
clockwise.
Caution: The blade is quite sharp. Please act with caution to avoid cuts.
Replacement of Spare Parts
Only original spare parts are allowed.
Servicing
Please contact your local dealer.
The BLooK LED transilluminator is warranted against defects in materials and
workmanship for 1 year. If any defects occur in the instrument or accessories during this
warrant period, GeneDireX will repair or replace the defective parts at its discretion
without charge. The following defects, however, are specifically excluded:
1. Defects caused by improper operation.
2. Repair or modification done by anyone other than GeneDireX or an authorized agent.
3. Damage caused by substituting alternative parts.
4. Use of fittings or spare parts supplied by anyone other than GeneDireX.
5. Damage caused by accident or misuse.
6. Damage caused by disaster.
7. Corrosion caused by improper solvent or sample.
For any inquiry or request for repair service, contact your local GeneDireX office. Inform
the model and serial number of your instrument.

BLooK LED transilluminator complies with the European Community
Safety requirements.
A CE Marking is a European marking of conformity that indicates a
product complies with the essential requirements of the applicable
European laws or directives with respect to safety, health, and
environment and consumer protection. Generally, this conformity to the
applicable directives is done through self-declaration. The CE Marking
is required on products in the countries of the European Economic
Area (EEA) to facilitate trade among the member countries. The
manufacturer or their authorized representative established in the EEA
is responsible for affixing the CE Marking to their product. The CE
Marking provides a means for a manufacturer to demonstrate that a
product complies with a common set of laws required by all of the
countries in the EEA to allow free movement of trade within the EEA
countries.
Caution
Caution risk of danger.
Consult the User Guide for further safety information.
Do not dispose of this product in unsorted municipal waste.
Caution
To minimize negative environmental impact from disposal of electronic
waste, do not dispose of electronic waste in unsorted municipal waste.
Follow local municipal waste ordinances for proper disposal provision
and contact customer service for information about responsible disposal
options.
REGULATORY NOTICE
IMPORTANT: This GeneDireX instrument is designed and certified to meet safety
standards. Certified products are safe to use when operated in accordance with the
instruction manual. This instrument should not be modified or altered in any way.
Alteration of this instrument will:
1. Void the manufacturer’s warranty
2. Void the safety and EMC certification
3. Create a potential safety hazard
GeneDireX is not responsible for any injury or damage caused by the use of this
instrument for purposes other than those for which it is intended, or by modifications of
the instrument not performed by GeneDireX or an authorized agent.
Appendix
Explanation of Symbols and Warnings
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Caution
The BLooK LED transilluminator is classified as a Class 2 LED
product, which is indicated by the symbol to the left, radiation when
open, do not stare into the beam
Safety Information
The BLooK LED transilluminator is an electrical device. Never touch the power cord or
outlet with wet hands. Do not use this device in damp areas or while standing on damp
floors. Do not attempt to open the BLooK LED transilluminator. The BLooK LED
transilluminator should be used with the supplied power cord. Only these power cords
should be used to power the device.Attach the power cord to the BLooK LED transilluminator
at the back of the device. Plug the other end of the power cord into a properly grounded
electrical outlet, ensuring the correct plug adaptor is attached. Always disconnect the
BLooK LED transilluminator from the electrical outlet before cleaning the device.
The BLooK LED transilluminator does not produce UV- light; however, it does utilize an
intense blue light for viewing gels. It should be noted that published literature has
identified blue light as a possible risk factor for macular degeneration; however, no
clinical studies have been published. Therefore, the BLooK LED transilluminator amber
filter unit provided with this device should be used to protect your eyes while viewing gels.
The amber filter unit is NOT a safety screen for UV emission, and will NOT protect your
eyes when viewing gels on UV transilluminators. After viewing and documenting the gel
or sample, always press the unit off.
Warranty
GeneDireX warrants that BLooK LED transilluminator will be free from defects in
material and workmanship for a period of one year from date of purchase. If a defect is
present, GeneDireX will, at its option, repair, replace, or refund the purchase price of
this product at no charge to you, provided it is returned during the warranty period. This
warranty does not apply if the product has been damaged by accident, abuse, misuse
or misapplication, or from ordinary wear and tear. This warranty shall be limited to the
replacement of defective products. It is expressly agreed that this warranty will be in lieu
of all warranties of fitness and in lieu of the warranty of merchantability.

Made in TAIWAN
CHINA Patent: CN203115668U TAIWAN Patent: M449283