Grant-bio ES-20 User manual

Operating instructions
Shaker-Incubator
ES-20
For version V.2GD

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Operating instructions
Version 2.02 - July 2013

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Contents
1 Safety ...........................................................................................4
2 General Information....................................................................5
3 Getting Started ............................................................................6
4 Operation of ES-20......................................................................7
5 Specifications..............................................................................9
6 Guarantee and service..............................................................10
7Declaration of Conformity ........................................................11
ES-20
Operating instructions Version 2.02 - July 2013

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The following symbols mean:
Caution! Make sure you have fully read and understood the present operating
instructions before using the equipment. Please pay special attention to
sections marked by this symbol.
GENERAL SAFETY
Use only as specified in the operating instructions provided.
The unit should not be used if dropped or damaged.
The unit must be stored and transported only in an horizontal position (see marking on the
package).
After transportation or storage keep the unit under room temperature for 2–3 hrs before
connecting it to the electric circuit.
Use only cleaning and decontamination methods recommended by the manufacturer.
Do not make modifications in design of the unit.
ELECTRICAL SAFETY
Connect only to electric circuit with voltage corresponding to that on the serial number label.
Ensure that the electric circuit switch and the plug are easily accessible during use.
Disconnect the unit from the electric circuit before moving.
Disconnect the power cord plug from power socket to turn off the unit.
After moving the unit check the shaker control cable connections.
Do not plug the unit into an ungrounded the power socket, and do not use an ungrounded
extension lead.
If liquid penetrates into the unit, disconnect it from the electric circuit and have it checked by a
repair and maintenance technician.
1. Safety
ES-20
Operating instructions
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ES-20
Operating instructions
DURING OPERATION
Do not impede the platform motion.
Do not operate the unit in premises with aggressive or explosive chemical mixtures.
Do not disconnect the shaker control cable when the unit is switched on.
Do not operate the unit if it is faulty or has been installed incorrectly.
Do not use outside laboratory rooms.
Do not place a load exceeding the maximum load value mentioned in the specifications section
of these operating instructions.
BIOLOGICAL SAFETY
It is the user's responsibility to carry out appropriate decontamination if hazardous material is
spilt on or penetrates into the equipment.
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2. General Information
The ES-20 is a compact desktop shaker-incubator used for mixing of biological liquids as well as
incubation and cultivation of biological liquids according to the program set by the operator.
Built-in microprocessor thermocontroller provides constant temperature control in the incubator
chamber. Forced heated air circulation inside the transparent plexiglass chamber guarantees even
temperature distribution. Dismountable construction makes transportation easy.
Orbital shaking is controlled by the digital tachometer (rpm) and digital timer regardless of the
temperature. The unit is equipped with the direct-drive system ensuring most reliable stable long-time
operation (up to 30 24 hour cycles).
The ES-20 is easy to operate. The two-line LCD display shows both set and actual values for
temperature, time and speed.
The device consists of a thermal regulator and control block, shaking block and a plexiglass
chamber.
The following elements are located on the front panel:
-power switch (fig.1/ );
-display (fig.1/ );
-control buttons (fig.1/ ).
The following elements are located on the rear panel:
-shaking block control cable (fig.1/ );
-socket for connecting the power cord (fig.1/ );
Fig.1 Unit construction
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ES-20
Operating instructions
- ).
Five interchangeable platform types allow using the shaker for:
-growing cell cultures in flasks and other laboratory glassware;
-extracting tissue samples at physiological temperatures;
-other sample preparation processes.
The unit is applicable in all the areas of clinical, biotechnology and microbiology laboratory
research.
fuse holder (fig.1/
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Operating instructions
u PUP-12 v PP-4 w P12-100
y P16-88 z HB-200
3. Getting started
3.1 Unpacking
Remove packaging carefully, and retain them for future shipment or storage of the unit
Examine the unit carefully for any damage incurred during transit. The warranty does not
cover in-transit damage.
3.2The ES-20 set includes
• Shaker-Incubator ES-20 ....................................................................................1 piece
• Power cord..........................................................................................................1 piece
• Control cable.......................................................................................................1 piece
• Spare fuse ..........................................................................................................1 piece
• Operating Instructions; Declaration of Conformity..................................................1 copy
• ES-20 Shaker-Incubator Assembling Instruction ...................................................1 copy
Optional accessories:
·PUP-12 platform u........................................................................................on request
·PP-4 platform v.............................................................................................on request
·P12-100 platform w.......................................................................................on request
·P6-250 platform x.........................................................................................on request
·P16-88 platform y.........................................................................................on request
·HB-200 holding bar z....................................................................................on request
x P6-250
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ES-20
Operating instructions
3.3. Set up:
-Assemble the unit using the enclosed ES-20 Orbital Incubator Shaker Assembling
Instruction as a guide;
Note! ES-20 Orbital Incubator Shaker Assembling Instruction can be found on our web
page, please visit the link http://distributor.grant.co.uk/
media/19989/assembling%20ES-20_en6_19102012.pdf.
-Place the unit on a horizontal even working surface;
-Remove protective film from the display;
-Connect the shaker control cable (fig.1/x) into the corresponding sockets on the rear
of the thermostatic and shaking blocks;
-Plug the mains cable into the socket on the rear (fig.1/y) and position the unit so that
there is easy access to the power switch and connector.
3.4. Platform installation:
Install the platform by inserting the pins on the bottom side of the platform into the holes
on the supporting platform on the shaker.
Note! Move the unit carefully and only in a vertical position.
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Operating instructions
4. Operation of ES-20
4.1. Connect the unit to a grounded power socket. Set the Power switch to position I (ON).
4.2. The display will turn on with the upper line (Set p.) showing the previously set time, speed
and temperature and the lower line (Actual p.) showing current readings of the same
parameters (STOP – time, 000 – rpm, incubator temperature °C, which automatically starts
rising according to the temperature set in the upper line).
Setting the parameters
Use the readings in the upper line of the display (Set p.), while setting the parameters
required.
Setting time (Time)
4.3. Using the p and q Time keys (Fig. 2/u) set the required working time interval in hours and
minutes (increment - 1 min). Pressing the key for more than 3 sec will increase the increment.
Setting speed (RPM)
4.4. Using the p and q RPM keys (Fig. 2/v) set the required shaking speed (increment 10
RPM). Pressing the key for more than 3 sec will increase the increment.
Setting temperature(Temp.ºC)
4.5. Using the p and q Temp. keys (Fig. 2/w) set the required temperature (increment 0.1ºC).
Pressing the key for more than 3 sec will increase the increment.
Fig.2 Control panel
Shaker
Timer
Time RPM Temp.
PROCEEDING PARAMETERS SET PARAMETERS
Time (hr : min) RPM Temp. °C
Set p.
Actual p. Run/Stop
STOP 000 +37.0
+37.0
160 00:25
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ES-20
Operating instructions
Caution! The chamber heating can be turned off only by setting the temperature value below
25 °C (the display will show OFF – Temp.ºC– Set p.).
The set parameters can also be changed during operation.
Program execution
4.6. Place samples on the platform.
4.7. Press the Shaker-Run/Stop key (Fig. 2/x). The platform will start rotating and the timer
indicator will start counting up the time interval (with 1 min precision).
Note! If the rotation speed is set to zero, pressing Shaker-Run/Stop key starts the timer but
the platform does not move.
4.8. After finishing the program (after the set time elapses) the platform motion will stop and the
timer will be showing the flashing reading STOP accompanied by the repetitive sound signal
until the Shaker-Run/Stop key is pressed.
Caution! At the end of the set time period the platform movement is stopped automatically,
but the heating can be stopped ONLY manually by reducing the temperature using
the q Temp. key (Fig. 2/w – the lower key) till the OFF sign appears in the upper
line of the display.
4.9. If the working time is not set (the timer indicator in the upper line shows 00:00), pressing the
Shaker-Run/Stop key will start continuous operation of the unit (with countdown timer in the
lower line and indication OFF in the upper line) until the Shaker-Run/Stop key is pressed
again.
4.10. The timer can be reset during operation if required. Press theTimer-Run/Stop key twice
(Fig. 2/y) (first – to stop the timer, second – to restart the timer).
4.11. The platform motion can be stopped at any time by pressing the Shaker-Run/Stop key. In
this case the program execution and the platform motion will stop and the timer will switch
into the STOP mode saving previously set time. Press the Shaker-Run/Stop key to repeat
the operation with the same time and speed.
4.12. After finishing the operation set the power switch, located on the rear panel of the unit, in
position O (Off) and disconnect the external power supply with from electric circuit.
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5. Specifications
The product is designed for operation indoors in a laboratory at altitudes up to 2000 m, with
ambient temperature from +4°C to +40°C and maximum relative humidity 80% for temperatures
up to 31°C decreasing linearly to 50% relative humidity at 40°C.
·Temperature setting range ........................................................................+25°C ... +42°C
·Temperature control range ...................................................5°C above ambient ... +42°C
·Temperature setting resolution ..................................................................................0.1ºC
·Speed control range ......................................................50–250 RPM (increment 10 rpm)
·Maximum load...........................................................................................................2.5 kg
·Orbit ........................................................................................................................10 mm
·Digital time setting...........................................................1 min – 96 hrs (increment 1 min)
·Continuous operating time, not more ....................................................................30 days
Recommended interval between operation sessions not less than 8 hours
·Display .....................................................................................................2x16 signs, LCD
·Dimensions ............................................................................................340x340x435 mm
·Current/power consumption .........................230 V, 0.7 A / 160 W or 120 V, 1.6 A / 170 W
·Voltage .......................................................................230 V, 50/60 Hz or 120 V, 50/60 Hz
·Weight*....................................................................................................................13.2 kg
* Accurate within ±10%.
Grant is committed to a continuous programme of improvement, specifications may be changed without
notice.
Description
Optional
accessories
Metallic flat platform with non-slip rubber mat. Working dim. 215x215 mm PP-4
Universal platform with adjustable bars for different types of flasks,
with non-slip rubber mat. Working dim. 265x185 mm
PUP-12
Additional holding bar for PUP-12HB-200
Platform with clamps for flasks, 100 ml (12 places) Working dim. 250x190 mmP12-100
Platform with clamps for flasks, 250 ml (6 places) Working dim. 250x190 mm
P6-250
Platform with spring holders for up to 88 tubes up to 30 mm diameter
(e. g. 10 ml, 15 ml, 50 ml tubes) Working dim. 275x205x75 mm
P16-88
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ES-20
Operating instructions
6.1 Guarantee
When used in laboratory conditions and according to these working instructions, this
product is guaranteed for TWO YEARS against faulty materials or workmanship.
6.2 Service
There are no user-serviceable parts inside the unit. For all maintenance and repairs (except
as defined below) return to our service department in the UK or in other countries, our
distributor.
Cleaning
The door and side panels are made of organic glass (polymethyl methacrylate) Plexiglass®)
and are prone to scuffing and scratches if improperly cleaned. Care during cleaning of the
door and side panels is recommended to reduce wear of the Plexiglass surface.
Caution! Clean the door and side panels with a mild detergent; never use organic based
compounds, pure alcohol, alcohol-containing cleaners (more than 15%) or
ammonia containing cleaners for Plexiglass! Do not use abrasive pads or
cleansers.
For decontamination, it is recommended to use a special DNA/RNA removing solution (e.g.
TM TM
DNA-Exitus Plus , RNase-Exitus Plus ).
The table below shows the interreaction of Plexiglass with ethyl alcohol and other solutions.
Liquid Interreaction with Plexiglass
DNA-Exitus PlusTM No reaction.
RNase-Exitus PlusTM No reaction.
Ethyl alcohol 10-15% No reaction.
Ethyl alcohol 30% Limited reaction.
Ethyl alcohol Pure Full reaction. Do not use!
H O 6% No reaction.
2 2
Standard ethanol (75%) or other cleaning agents are recommended for cleaning and
disinfection of the stainless steel surfaces.
6. Guarantee and Service
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Operating instructions
Fuse replacement
Disconnect the unit from the mains. Disconnect the power cable from the socket on the rear
of the shaker-incubator. Open the fuse holder (fig.1/z). Replace with the correct fuse (for
230 V, – T1 A, for 120 V – T2 A).
6.3 Routine safety tests
If routine tests are to be made, we recommend a test of the integrity of the protective earth
conductor and an insulation test at 500 Vdc. Routine flash tests are not recommended for
any electrical equipment, because repeated high voltage tests degrade insulation materials.
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ES-20
Operating instructions Version 2.02 - July 2013

Shaker-Incubator/ES-20/28711/2.02
Grant Instruments
(Cambridge) Ltd
Shepreth
Cambridgeshire
SG8 6GB
UK
Tel: [phone]
Fax: [phone]
Email: [email protected]
www.grantinstruments.com