Hanna Instruments HI 83749 User manual

INSTRUCTION MANUAL
HI 83749
Turbidity & Bentocheck

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the
copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using this meter.
This manual will provide you with the necessary information for correct use of this meter,
as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
[email protected] or view our worldwide contact list at www.hannainst.com.
Dear
Customer,

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TABLE OF CONTENTS
TABLE OF CONTENTS
1. PRELIMINARY EXAMINATION ............................................................................................... 5
2. GENERAL DESCRIPTION....................................................................................................... 6
2.1. BENTOCHECK......................................................................................................... 6
2.2. TAG IDENTIFICATION SYSTEM.................................................................................. 7
2.3. ABBREVIATIONS..................................................................................................... 8
3. SPECIFICATIONS................................................................................................................. 9
4. FUNCTIONAL DESCRIPTION................................................................................................ 10
4.1. INSTRUMENT DESCRIPTION .................................................................................. 10
4.2. KEYPAD DESCRIPTION........................................................................................... 10
4.3. CONNECTORS DESCRIPTION.................................................................................. 11
4.4. DISPLAY DESCRIPTION.......................................................................................... 12
4.5. BEEPER............................................................................................................... 12
5. GENERAL TIPS FOR AN ACCURATE MEASUREMENT............................................................... 13
5.1. CUVETTE.............................................................................................................. 13
5.2. CUVETTE HANDLING ............................................................................................. 13
5.3. CUVETTE OILING................................................................................................... 13
5.4. SAMPLING TECHNIQUE......................................................................................... 13
5.5. REMOVING AIR BUBBLES...................................................................................... 14
5.6. MEASUREMENT TIPS............................................................................................ 14
6. CALIBRATION PROCEDURE................................................................................................. 15
6.1. CALIBRATION....................................................................................................... 15
6.2. OUT OF CALIBRATION RANGE FUNCTION................................................................ 17
6.3. CALIBRATION ERRORS .......................................................................................... 18
6.4. DELETE CALIBRATION............................................................................................ 18
7. TURBIDITY MEASUREMENT ............................................................................................... 19
7.1. AVG (SIGNAL AVERAGE MODE) .............................................................................. 20
7.2. CONTINUOUS MEASUREMENT............................................................................... 20
8. BENTOCHECK (PROTEIN STABILITY TEST)............................................................................ 21
9. DETERMINATION OF BENTONITE REQUIREMENT ................................................................. 24
9.1. SAMPLE PREPARATION ......................................................................................... 24
9.2. READING PROCEDURE .......................................................................................... 26
9.3. COMPARATIVE MODE............................................................................................ 28
10. LOGGING........................................................................................................................ 29
10.1. VIEW LOGGED DATA............................................................................................ 30
11. GOOD LABORATORY PRACTICE (GLP)................................................................................. 33

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TABLE OF CONTENTS
12. SETUP........................................................................................................................... 35
12.1. SET REFERENCE VALUE....................................................................................... 35
12.2. ACTIVATING COMPARATIVE MODE........................................................................ 36
12.3. SET EPA COMPLIANCE MODE............................................................................... 37
12.4. SET BEEPER....................................................................................................... 38
12.5. SHOW / HIDE THE TIME ..................................................................................... 38
12.6. SET THE DATE.................................................................................................... 39
12.7. SET THE TIME .................................................................................................... 40
12.8. SET INSTRUMENT ID .......................................................................................... 41
12.9. SET BAUD RATE ................................................................................................. 42
12.10. LCD BACKLIGHT................................................................................................ 42
13. TAG INSTALLATION ......................................................................................................... 43
14. LAMP REPLACEMENT....................................................................................................... 44
15. BATTERY MANAGEMENT.................................................................................................. 45
15.1. BATTERIES REPLACEMENT................................................................................... 46
15.2. USING AN AC ADAPTER....................................................................................... 46
16. ERROR CODES................................................................................................................ 47
17. PC INTERFACE ................................................................................................................ 48
18. ACCESSORIES................................................................................................................. 49
CERTIFICATION...................................................................................................................... 50
RECOMMENDATIONS FOR USERS............................................................................................ 50
WARRANTY ........................................................................................................................... 50

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PRELIMINARY EXAMINATION
1. PRELIMINARY EXAMINATION
Remove the meter from the packing material and examine it carefully. For further assistance, please
contact your local Hanna Instruments office or email us at [email protected].
This HI83749 Turbidity & Bentocheck Meter is supplied complete with:
• Six sample cuvettes and caps
• Four calibration cuvettes (HI83749-11)
• Bentocheck reagent (HI83749-0) and Silicone oil (HI93703-58)
• One 1000 µL automatic pipette with two tips and Instructions sheet
• Four 25 mL glass vials with caps
• One 1 mL syringe with two tips; one Funnel; Filter paper (25 pieces)
• Five tag holders with tags (HI920005)
• Tissue for wiping the cuvettes
• Four 1,5V AA batteries
• AC Adapter
• Instruction manual
• Instrument quality certificate
• Rugged carrying case
Note:Save all packing material until you are sure that the instrument works correctly. Any
damaged or defective item must be returned in the original packing with the supplied accessories.

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GENERAL DESCRIPTION
2. GENERAL DESCRIPTION
The HI83749 is an auto diagnostic microprocessor meter that benefits from Hanna’s years of
experience as manufacturer of analytical instruments.
The meter is especially designed for wine analyses and, beside turbidity measurements, it allows to
make tests to verify protein stability (e.g. Bentocheck).
The instrument compensates wine color to guarantee accurate readings during the vinification process;
also for the darkest red wine samples. The optical system, consisting of a tungsten filament lamp
and multiple detectors, assures long term stability and minimizes the need of frequent calibration.
Calibration can be anyway easily performed at any time in two, three or four points (<0.1, 15, 100
and 500 NTU-adjustable calibration points), using the supplied or user prepared standards.
The meter has all necessary GLP (Good Laboratory Practice) functions to allow maximum traceability of
data like a real time clock, log-on-demand (up to 200 measurements), and T.I.S. -Tag Identification
System to give all recorded data a location, time and date stamp.
The meter measures turbidity of samples from 0.00 to 1200 NTU (Nephelometric Turbidity Units) and
is USEPA compliant. In the USEPA measurement mode the instrument rounds the readings to meet
USEPA reporting requirements. It has a continuous measurement mode to verify the settling rate of
suspended matter, and a signal average (AVG) mode to accumulate multiple readings giving a final
average value. The AVG routine is particularly useful to measure samples with suspended particles
with different dimensions.
The HI83749 has a user-friendly interface, with a large backlit LCD (Liquid Crystal Display). Acoustic
signals and displayed codes to guide the user step by step through routine operations.
This valuable splash proof portable turbidity meter is supplied in a rigid carrying case that offers
protection for harsh environments.
2.1. BENTOCHECK
The prevention of protein haze or deposit in bottled white wines is a universal concern and often a wine
needs to be stabilized before bottling. One commonly used stabilization agent is bentonite. Bentonite
is a volcanic clay earth type fining agent (like kaolin). It improves the clarity and stability of wine
but has also negative aspects because of the volume of lees formed, reduction of tannin and color.
Since there are different types and qualities of bentonite with different capacity of protein removal, it
is important to make laboratory trails with the same lot and wetting degree of the bentonite as that
will be used in the cellar.
Protein stabilization is normally not a problem in bottled red wines because of the relatively high
concentration of phenols that binds and precipitates with the instable proteins before bottling. Often
bentonite is added to red wines at a level of about 12 g/hL (1 lb/1000 gal), reducing colloidal
suspended particles thus improving membrane filterability.
Wines with low phenols contents, such as rose, light reds and whites should be checked for protein
stability before bottling. Hanna is offering a rapid test to verify the risk of future protein haze formation.

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GENERAL DESCRIPTION
If protein instability is detected, a next test can help defining the right amount of bentonite to be
added for improving protein stability. It is important not to overdose bentonite, avoiding stripping
wine flavour, body, and significant loss of color, especially in young red wines. Moreover, adding only
the necessary amount of bentonite to obtain the desired protein stability is also costs saving.
2.2. TAG IDENTIFICATION SYSTEM
Hanna is the first manufacturer of turbidity instruments that has decided to add the unique T.I.S. - Tag
Identification System to portable turbidity meters, to meet the more restrictive needs for traceability
and data management of our clients.
The system is designed for scientific and industrial applications, or to prove during safety audits and
inspections that samples have been truly taken on pre-established locations.
The system is as easy to install as to operate. Just fix the so-called iButton® tags near your sampling
sites that need to be checked often, and with this the T.I.S. is setup. The tag contains a computer chip
embedded in a durable stainless steel can. It is designed to withstand the harsh environments, indoors
or outdoors. The number of tags that can be installed is practically unlimited, because each tag has
a unique identification code.
Immediately after installation of the tags you can start collecting data. Use the meter to take
measurements and memorize the test result by pressing the Log-on-Demand key. Then, the instrument
will ask for the tag identification. Simply touching the iButton® with the matching connector on the
meter does identify and authenticate logging, by storing the iButton® serial number, time and date
stamp events.
The power of the T.I.S. features resides in the PC application. Download all test data to your PC and
use our HI92000 Windows® compatible application software for further data management. You can
sort or filter all your collected data on different criteria like on a specific sampling location, parameter,
date and time intervals, or fix range to filter measured values. The data can be plotted in a graph,
exported to other common Windows® applications or printed for reporting purpose.
It is also possible to add new tags later on, thus increasing an already existing database. Each
time the PC software recognizes a not already registered tag, it will ask for a description of the new
sampling location.

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GENERAL DESCRIPTION
2.3. ABBREVIATIONS
NTU Nephelometric Turbidity Units
FTU Formazin Turbidity Units
USEPA US Environmental Protection Agency
LCD Liquid Crystal Display
RTC Real Time Clock
T.I.S. Tag Identification System
iButton® is registered Trademark of “MAXIM/DALLAS semiconductor Corp.”
Windows® is registered Trademark of “MICROSOFT Corporation”
A light beam that passes through the sample is scattered in all directions. The intensity and pattern
of the scattered light is affected by many variables like wavelenght of the incident light, particle size,
shape, refractive index and color.
The optical system includes a tungsten filament lamp, a scattered light detector (900) and a
transmitted light detector (1800). The microprocessor of the instrument calculates the NTU value from
the signals that reaches the two detectors.

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SPECIFICATIONS
3. SPECIFICATIONS
Range 0.00 to 9.99 NTU
10.0 to 99.9 NTU
100 to 1200 NTU
Range Selection Automatically
Resolution 0.01 NTU from 0.00 to 9.99 NTU
0.1 NTU from 10.0 to 99.9 NTU
1 NTU from 100 to 1200 NTU
Accuracy ±2% of reading plus 0.05 NTU
Repeatibility ±1% of reading or 0.02 NTU, whichever is greater
Stray Light < 0.05 NTU
Light Source Tungsten filament lamp
Light Detector Silicon Photocell
Method Ratio Nephelometric Method.
Display 60 x 90mm backlit LCD
Calibration Two, three or four points calibration
LOG Memory 200 records
Serial Interface RS232 or USB 1.1
Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing
Power supply 4 x 1.5V AA alkaline batteries or AC adapter
Auto Shut-off After 15 minutes of non-use
Dimensions 224 x 87 x 77 mm (8.8 x 3.4 x 3.0”)
Weight 512 g (18 oz.)

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FUNCTIONAL DESCRIPTION
4. FUNCTIONAL DESCRIPTION
4.1. INSTRUMENT DESCRIPTION
1. Cuvette lid
2. Cuvette holder
3. Backlit Liquid Crystal Display (LCD)
4. Splash proof keypad
5. Lamp connector
6. Lamp holder
7. Battery compartment
4.2. KEYPAD DESCRIPTION
8. ON/OFF - press to turn the instrument ON or to activate the back light. Keep the key pressed for
three seconds to turn the instrument OFF.