Hanna Instruments HI97105 User manual

INSTRUCTION MANUAL
Hanna Instruments Inc., 584 Park East Drive, Woonsocket, RI 02895 USA www.hannainst.com
HI97105 | HI97115
XpH
XAlkalinity
XAmmonia
XCalcium
XMagnesium
XNitrate Low Range
XNitrate High Range
XNitrite Ultra Low Range
XPhosphate Ultra Low Range
Marine Master Waterproof
Multiparameter Photometer

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner,
Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Hanna Instruments reserves the right to modify the design, construction, or appearance of its products without advance notice.
TABLE OF CONTENTS
1. Preliminary Examination ...........................................3
2. Safety Measures.......................................................4
3. Specifications...........................................................4
3.1. Photometer Specifications ................................4
3.2. Measurement System.......................................4
3.3. Methods.........................................................5
4. Description...............................................................6
4.1. General Description & Intended Use ..................6
4.2. Functional Description......................................7
4.3. Precision & Accuracy........................................8
4.4. Principle of Operation ......................................8
4.5. Optical System................................................8
5. General Operations...................................................9
5.1. Meter Validation: CAL Check™.........................9
5.2. Chemical Formula & Unit Conversion...............11
5.3. Logging Data & Log Recall .............................11
5.4. General Setup ...............................................12
5.5. Reagents & Accessories..................................15
5.6. Instruction Manual ........................................15
5.7. Contextual Help.............................................15
5.8. Bluetooth Connectivity, HI97115 only.............16
5.9. Hanna Lab Application...................................16
6. Photometer............................................................17
6.1. Method Selection...........................................17
6.2. Reading Location Selection.............................17
6.3. Collecting & Measuring Samples and Reagents...18
6.4. Cuvette Preparation .......................................18
6.5. Measurement Recommendations.....................19
6.6. Battery Management.....................................20
7. Method Procedure...................................................21
7.1. Marine pH ....................................................21
7.2. Marine Alkalinity...........................................22
7.3. Marine Ammonia...........................................23
7.4. Marine Calcium.............................................25
7.5. Marine Magnesium........................................27
7.6. Marine Nitrate LR..........................................28
7.7. Marine Nitrate HR .........................................31
7.8. Marine Nitrite ULR.........................................32
7.9. Marine Phosphate ULR...................................33
8. Warning & Error Descriptions...................................35
9. Battery Replacement...............................................36
10. Accessories...........................................................37
Certification.................................................................38
Recommendations for Users..........................................38
Warranty ....................................................................38
Abbreviations..............................................................38
Regulatory Notices, HI97115 only ................................39
Dear Customer,
Thank you for choosing a Hanna Instruments®product.
This manual has been written for:
• HI97105 photometer with software version v1.04 and higher
• HI97115 photometer with software version v1.03 and higher
Please read this instruction manual carefully before using these instruments as it provides the necessary
information for correct use of these instruments as well as a precise idea of their versatility.
If you need additional technical information, do not hesitate to e‑mail us at [email protected].
Visit www.hannainst.com for more information about Hanna Instruments and our products.

3 Preliminary Examination
1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully. For further assistance,
please contact your local Hanna Instruments®office or email us at [email protected].
Each HI97105 or HI97115 is supplied with:
• Sample cuvette (2 pcs.)
• Sample cuvette cap (2 pcs.)
• 1.5V AA Alkaline batteries (3 pcs.)
• Instrument quality certificate
• Quick reference guide with instructions for manual download
Each HI97115C* is delivered in a rugged carrying case and is supplied ** with:
• Sample cuvette (2 pcs.)
• Sample cuvette cap (2 pcs.)
• Marine pH reagent, 30 mL dropper (1 pc.)
• Marine Alkalinity reagent, 30 mL bottle (1 pc.)
• Marine Ammonia starter kit
Reagent A, 30 mL bottle (1 pc.)
Reagent B and C (reagent for 25 tests, each)
• Marine Calcium starter kit
Reagent A, 30 mL bottle (1 pc.)
Reagent B (reagent for 25 tests)
• Marine Magnesium starter kit
Reagent A, 120 mL bottle (1 pc.)
Magnesium Indicator reagent (for 25 tests)
• Marine Nitrate High Range reagent (for 25 tests)
• Marine Nitrite Ultra Low Range reagent (for 25 tests)
• Marine Phosphate Ultra Low Range reagent (for 25 tests)
• 1 mL graduated syringe with tip (3 pcs.)
• Minipipette with tip (1 pc.)
• 3 mL Pasteur pipette (2 pcs.)
• 5 mL syringe with black printing and tip (1 pc.)
• 5 mL syringe with blue printing and tip (1 pc.)
• 1.5V AA Alkaline batteries (3 pcs.)
• Cloth for wiping cuvettes
• Scissors
• Instrument quality certificate
• Quick reference guide with instructions for manual download
Note: Save all packing material until you are sure that the instrument works correctly. Any damaged or defective
item must be returned in its original packing material with the supplied accessories.
* HI97115UC, instrument code in the USA.
** Marine Nitrate Low Range testing reagent not supplied.

Safety Measures 4
2. SAFETY MEASURES
The chemicals contained in the reagent kits may be hazardous if improperly handled.
Read the Safety Data Sheets (sds.hannainst.com) before performing tests.
Safety equipment • Follow instructions carefully and wear suitable eye protection and clothing when
required.
Reagent spills • If a reagent spill occurs, wipe up immediately and rinse with plenty of water.
• If reagent contacts skin, rinse the affected area thoroughly with water.
• Avoid breathing released vapors.
Waste disposal • Contact a licensed waste disposal provider for proper disposal of reagent kits and
reacted samples.
3. SPECIFICATIONS
3.1. PHOTOMETER SPECIFICATIONS
Auto logging 200 readings
Display 128 x 64 pixel B/W LCD with backlight
Auto-off After 15 minutes of inactivity
(after 30 minutes of inactivity if a Zero has been done but not a Read)
Battery type 1.5 V AA Alkaline (3 pcs.)
Battery life > 800 measurements (without backlight)
Environment 0 to 50 °C (32 to 122 °F)
0 to 100 % RH, non‑serviceable
Dimensions 142.5 x 102.5 x 50.5 mm (5.6 x 4.0 x 2.0")
Weight 380 g (13.4 oz.), with batteries
Casing IP67 rating, floating
3.2. MEASUREMENT SYSTEM
Light source LED
Bandpass filter
Wavelength 525 nm & 610 nm
Bandwidth 8 nm
Wavelength accuracy ±1.0 nm
Light detector Silicon photocell
Cuvette type Round 24.6 mm diameter (22 mm inside diameter)

5 Specications
3.3. METHODS
Range Resolution Accuracy
at 25 °C (77 °F)
LED
Description
pH
6.3 to 8.6
pH
0.1
pH
±0.2 pH
of reading
525 nm
Colorimetric Adaptation of
Phenol Red Method.
Alkalinity
0.0 to 20.0
dKH
0.1
dKH
±0.3 dKH
±5 % of reading
610 nm
Colorimetric Method.
Reaction causes a distinctive range of
colors from yellow to greenish blue to
develop.
Ammonia
0.00 to 2.50
ppm
(as NH3)
0.01
ppm ±0.05 ppm
±5 % of reading
610 nm
Adaptation of the Salicylate Method.
The Reaction between Ammonia and
Ammonium and the reagent causes a
blue‑green tint in the sample.
Calcium
200 to 600
ppm
1
ppm ±6 % of reading
610 nm
Adaptation of Zincon Method.
Magnesium
1000 to 1800
ppm
(as Mg2+)
5
ppm ±5 % of reading
610 nm
Adaptation of Colorimetric EDTA Method
using calmagite indicator.
Reaction between magnesium and the
reagents causes a blue to violet tint in the
sample.
Nitrate LR
0.00 to 5.00
ppm
(as NO3¯)
0.01
ppm
±0.25 ppm
±2 % of reading
525 nm
Zinc Reduction Method.
Reaction between nitrate and the reagent
causes a pink tint in the sample.
Nitrate HR
0.0 to 75.0
ppm
(as NO3¯)
0.1
ppm
±2.0 ppm
±5 % of reading
525 nm
Zinc Reduction Method.
Reaction between nitrate and the reagent
causes a pink tint in the sample.
Nitrite ULR
0 to 200
ppb
(as NO2¯‑ N)
1
ppb ±10 ppb
±4 % of reading
525 nm
Adaptation of EPA Diazotization Method
354.1. Reaction between nitrite and the
reagent causes a pink tint in the sample.
Phosphate ULR
0.00 to 0.90
ppm
0.01
ppm
±0.02 ppm
±5 % of reading
610 nm
Adaptation of Standard Methods for
Examination of Water and Wastewater,
20th Edition, Ascorbic Acid Method.
Reaction between phosphate and the
reagent causes a blue tint in the sample.

Description 6
4. DESCRIPTION
4.1. GENERAL DESCRIPTION & INTENDED USE
The HI97105 and HI97115 are waterproof multiparameter portable photometers that benefit from Hanna’s®
years of experience as a manufacturer of analytical instruments.
The instrument is a compact and versatile Marine multiparameter photometer designed to accurately determine
pH, Alkalinity, Ammonia, Calcium, Magnesium, Nitrate, Nitrite, and Phosphate levels in aquariums and marine
biology applications.
The photometer has an advanced optical system that uses a Light Emitting Diode and a narrow band interference
filter for accurate, repeatable readings. The optical system is sealed from outside dust, dirt, and water.
The meter uses an exclusive positive-locking system to ensure that the cuvettes are placed into the holder
in the same position every time.
With the CAL Check™ functionality, users are able to validate the performance of the instrument at any
time. Hanna Instruments®CAL Check cuvettes are certified against NIST‑traceable reference instrument(s).
The built-in tutorial mode guides users step‑by‑step through the measurement process. The tutorial mode
includes all steps required for sample preparation, the required reagents and quantities.
Suitable for field or bench measurements, the photometer features:
• Sophisticated optical system
• Meter validation using certified CAL Check cuvettes
• Tutorial mode guides the user step‑by‑step
• Option to assign locations to logged readings
• Auto logging
• Waterproof IP67, floating case
Operating Modes
The HI97115 can be used as a stand‑alone photometer or connected to the Hanna Lab App using Bluetooth
wireless technology and a compatible smart device.
The Hanna Lab App functions include calibration, measurement, data logging, graphing, and data sharing.

7 Description
4.2. FUNCTIONAL DESCRIPTION
Marine Master
Multiparameter Photometer
TANK1
Zero
Read
pH Marine
pH
8.0
у95%09:58:21
5
41
2
3
6
HI97115
204-810005
R
IC: 8595A-ANNAB1
Contains
FCC ID: XPYANNAB1
AS/NZS 4268:2012
R-C-ULX-ANNA-B112
ICASA TA-2019/1203
03850-19-05903
CCAI18LP2200T2
CMIIT ID: 2021DJ6698
Made in Romania
Woonsocket RI USA
3 x 1.5V AA
SN: XXXXXXXXXXXX
1. Liquid Crystal Display (LCD)
2. Keypad
3. ON/OFF power button
4. Cuvette holder
5. Indexing mark
6. Battery cover
Keypad Description
The keypad contains 3 direct keys and 3 functional keys with the following functions:
Press the functional key to perform the function displayed above it on the LCD.
Press and hold to power on/off. Press briefly to return to the previous screen.
Press to access the menu screen.
Press to display the context‑sensitive help menu.

Description 8
4.3. PRECISION & ACCURACY
Precision is how closely repeated measurements are to one another, usually expressed as standard deviation (SD).
Accuracy is defined as the closeness of a test result to the true value and is method specific.
Although good precision suggests good accuracy, precise results can be inaccurate.
Precise, accurate Precise, not accurate Not precise, accurate Not precise, not accurate
Figure 1: Precision versus Accuracy
4.4. PRINCIPLE OF OPERATION
Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and matter.
When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules, or crystal
lattices. Photometric chemical analysis is based on specific chemical reactions between a sample and reagent
to produce a light‑absorbing compound.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the
matter and on the physical‑chemical characteristics of the substance according to the Lambert‑Beer law.
If all other factors are constant, the concentration "c" can be calculated form the absorbance of the substance.
– log I/Io= elc d
or
A = elc d
Io= intensity of incident light beam
I= intensity of light beam after absorption
el= molar extinction coefficient at wavelength l
c = molar concentration of the substance
d = optical path through the substance
Figure 2: Lambert-Beer law
4.5. OPTICAL SYSTEM
LED
Filter
Beamsplitter
Reference Detector Microprocessor
Cuvette
Focusing Lens
Light Detector
Figure 3: Instrument Block Diagram

9 General Operations
The internal reference system (reference detector) of the photometer compensates for any drifts due to
power fluctuations or ambient temperature changes, providing a stable source of light for the blank (zero)
measurement and sample measurement.
LED light sources offer superior performance compared to tungsten lamps. LEDs have a much higher luminous
efficiency, providing more light while using less power. They also produce little heat, which could otherwise
affect electronic stability. LEDs are available in a wide array of wavelengths, whereas tungsten lamps have
poor blue/violet light output.
Improved optical filters ensure greater wavelength accuracy and allow a brighter, stronger signal to be received.
The end result is higher measurement stability and less wavelength error.
A focusing lens collects all of the light that exits the cuvette, eliminating errors from cuvette imperfections and
scratches, reducing the need to index the cuvette.
5. GENERAL OPERATIONS
5.1. METER VALIDATION: CAL CHECK™
Validation of the photometer involves verifying the concentration of the certified CAL Check standards*.
The CAL Check screen guides users step‑by‑step through the validation process.
Warning: Do not use any solutions or standards other than the Hanna Instruments® CAL Check standards.
For accurate validation, please perform it at room temperature, 18 to 25 ° C (64.5 to 77.0 ° F).
Note: Protect the CAL Check cuvettes from direct sunlight by keeping them in the original packing. Store between
5 and 30 ° C (41 to 86 ° F). Do not freeze.
To perform a CAL Check:
1. Press the key to enter the menu.
2. Use the functional keys to select CAL Check and press Select.
“Not Available“message or date, time, and status of the last CAL Check will be displayed on the screen.
Note: CAL Check is for the bandpass filter used by the selected method. Methods with the same bandpass filter
use the same CAL Check standards.
* CAL Check standards and testing reagents are sold separately. Please refer to Accessories section for ordering code.

General Operations 10
3. Press Check to start a new CAL Check.
Press the key at any time to abort the validation process.
4. Use the functional keys to enter the certificate value of the calibration
standard found on the CAL Check Standard Certificate.
5. Press Next to continue.
Note: This value will be saved in the instrument for future validation.
6. Insert the HI97100‑ZERO CAL Check Cuvette A then press Next to continue. “Please wait...“message will
be displayed during the measurement.
7. Insert the CAL Check Cuvette B for the selected method (i.e. HI97100‑525 for pH, Nitrate LR, Nitrate HR,
Nitrite ULR or HI97100‑610 for Alkalinity, Ammonia, Calcium, Magnesium, Phosphate ULR), then press
Next to continue. The “Please wait...“message will be displayed during the measurement.
Note: HI97100-ZERO, HI97100-525, and HI97100-610 are included in the HI97105-11 CAL Check™ standards
for Marine Master photometer – cuvette kit. Please see Accessories for ordering codes.
8. When the CAL Check is complete, the display will show one of the following messages and the value
obtained during the measurement:
• “PASSED“: measured value is within the accuracy specification
• “OUT OF SPECIFICATION“: measured value is outside of the tolerance window
A. Check the certified value, expiration date, and clean the outside of the cuvette.
B. Repeat the CAL Check procedure.
C. If this error continues, contact your nearest Hanna Instruments®customer service center.

11 General Operations
5.2. CHEMICAL FORMULA & UNIT CONVERSION
Chemical formula and unit conversion factors are method‑dependent and pre‑programmed into the instrument.
Note: When powered on, the instrument starts with the previously selected chemical form.
To view the displayed result in the desired chemical formula:
1. Press the key to enter the menu.
2. Use the functional keys to select Chemical Form (if available for selected method)
3. Press Select to change the displayed chemical formula.
4. Use functional keys to highlight desired chemical formula. Press Select.
5.3. LOGGING DATA & LOG RECALL
The instrument features a data autolog function to help users keep track of all
measurements. Every time a measurement is made the data is automatically
saved. The data log can hold 200 individual measurements.
Note: When the data log is full (200 data points), the meter will rewrite the oldest
data point. A confirmation message will display before a log is overwritten.
Viewing and deleting the data is possible using the Log Recall menu.
1. Press the key to enter the menu. Use the functional keys to select Log Recall and press Select.
2. Use the functional keys to highlight a log and press Info to view additional information about the log.
From this screen Next and Previous can be used to view other logs.
3. Press Delete to erase logged data. After pressing Delete a prompt on
display will ask for confirmation.

General Operations 12
4. Press No or the key to return to the previous screen. Press Yes to
delete selected log. Press Del All to erase all the logged data.
If Del All is pressed, follow the prompt to confirm. Press Yes to delete
all logged data, No, or the key to return to the log recall.
5.4. GENERAL SETUP
Press the key to enter the menu.
Use the functional keys to select Setup and press Select. Use the functional keys to highlight desired option.
Backlight
Option: 0 to 100 %
Press Modify to access backlight intensity.
Use the functional keys to increase or decrease the percentage intensity value.
Press Accept to confirm or the key to return to the Setup menu without saving the new value.
Contrast
Option: 0 to 100 %
Press Modify to change the display’s contrast. Use the functional keys to increase or decrease the percentage
contrast value.
Press Accept to confirm the value or the key to return to the Setup menu without saving the new value.
Date & Time
Press Modify to change the date and time. Press the functional keys to highlight the value to be modified (year,
month, day, hour, minute, or second).
Press Edit to modify the highlighted value. Use the functional keys to change the value.
Press Accept to confirm or the key to return to the previous screen.

13 General Operations
Time Format
Option: AM/PM, 24-hour
Press the functional key to select the desired time format.
Date Format
Press Modify to change the date format. Use the functional keys to select the desired format. Press Accept to
confirm or the key to return to the Setup menu without saving the new format.
Decimal Separator
Option: Comma ( , ) or Period ( . )
Press the functional key to select the desired decimal separator.
The decimal separator is used on the measurement screen.
Language
Press Modify to change the language. Use the functional keys to select the desired language.
Press Accept to choose one of the languages installed.
Beeper
Option: Enable, Disable
When enabled, a short beep is heard every time a key is pressed.
A long beep alert sounds when the pressed key is not active or an error is
detected. Press the functional key to enable or disable the beeper.
Locations
Option: 10, 25 (HI97105); 10, 25, 50, 75, 100 (HI97115)
This function allows users to edit between 10 (default) and up to 100 reading locations.
Press Modify and use the functional keys to set desired option.
Press Accept to confirm or the key to return to the Setup menu without saving.

General Operations 14
Tutorial
Option: Enable, Disable
Press the functional key to enable or disable the tutorial. When enabled,
the user will be guided step‑by‑step through the measurement procedures.
Bluetooth, HI97115 only
Option: ON, PAIR, OFF
Press Modify and use the functional keys to set desired option.
Press Accept to confirm or the key to return to the Setup menu without saving.
Once connected, the Bluetooth (&) icon is displayed in the top right corner of the screen.
Meter ID
Press Modify and use the functional keys to set desired ID.
Press Accept to confirm or the key to return to the Setup menu without saving the new Meter ID.
Meter Information
Press Select to view the model, serial number, firmware version, and selected language.
Press the key to return to the Setup menu.
Delete Paired Devices, HI97115 only
This function removes all previous Bluetooth connections.
Press Select to delete all paired devices. The meter will ask for confirmation.
Press Accept to confirm or Cancel to return to the Setup menu without performing the operation.

15 General Operations
Restore Factory Settings
Press Select to reset to factory settings.
Press Accept to confirm or Cancel to exit without restoring the factory settings.
5.5. REAGENTS & ACCESSORIES
Press the key to enter the menu.
Use the functional keys to select Reagents / Accessories and press Select to access a list of reagents and
accessories. Press the key to exit.
5.6. INSTRUCTION MANUAL
Press the key to enter the menu.
Use the functional keys to select Instruction manual and press Select see details on how to access the online
manual. Scan the QR‑code or use link to download the PDF. Press the key to exit.
5.7. CONTEXTUAL HELP
The photometer offers an interactive contextual help mode that assists the user at any time. Press the
key to access the help screen. The instrument will display additional information related to the current screen.
Use the functional keys to scroll the text and read all the available information.
Press the to exit help mode, or press the key to return to the previous screen.

General Operations 16
5.8. BLUETOOTH CONNECTIVITY, HI97115 ONLY
Using Hanna Lab App
To connect the photometer to the Hanna Lab App:
1. Power on the instrument and press Continue to enter measurement mode. &is displayed flashing indicating
the instrument is in discoverable mode.
Note: For Bluetooth connectivity, ensure Bluetooth option is configured as ON (default) in Setup. To disable,
configure as OFF.
2. On smart device: download and start the App. Grant required access.
Tap &and the instrument's ID appears in the list of Available Devices.
Tap “Connect” to enable Bluetooth connectivity. All readings are transmitted directly to the application.
Pairing a New Device
• With ON configured in Setup, the meter connects without pairing.
• With PAIR configured in Setup, a 6‑digit Bluetooth pin will be generated the first time the instrument
and the smart device are paired. Once the devices are paired, the pin is not required when reconnected.
Deleting Paired Devices
1. Press to select Delete Paired Devices option in Setup menu.
After selecting this option, a prompt on display is asking for confirmation.
2. Press Accept to confirm.
With PAIR option enabled, a bonding pin will need to be re‑entered when attempting a new Bluetooth connection.
5.9. HANNA LAB APPLICATION
• The Hanna Lab App is available from the App Store®and on Google Play.
• Consult the Help section of the application for information on measurement, data logging, graphing,
and data sharing.
• Measurements can be displayed alone, with tabulated data, or as a graph. The graph can be panned and
zoomed with pinch‑to‑zoom technology.
App Store is a trademark of Apple, Inc.
Google Play and the Google Play logo are trademarks of Google LLC.

17 Photometer
6. PHOTOMETER
6.1. METHOD SELECTION
1. Press the key to enter the menu.
2. Use the functional keys to select Methods and press Select.
3. Use the functional keys to highlight the desired method then press Select.
The instrument enters measurement screen.
• If tutorial mode is disabled, follow the measurement procedure.
• If tutorial mode is enabled, press Measure and follow the messages on the screen.
Note: At power on the instrument starts with the previously selected method.
6.2. READING LOCATION SELECTION
The user has the option to select a measurement location from up to 25 TANKS (HI97105) and from up to
100 TANKS (HI97115).
From menu, having previously selected required Method, press the corresponding functional key to select
a location.
Notes: Changing measurement location resets the Zero reading.
At power on the instrument starts with the previously selected location.
Editing Tank Name
1. From measurement screen press the corresponding functional key.
2. With option highlighted press Rename.
3. Use the functional keys to enter the name (up to 15 characters).
4. Press Accept to confirm.
5. Press the key to return to the measurement screen.

Cuvette Preparation 18
6.3. COLLECTING & MEASURING SAMPLES AND REAGENTS
Proper Use of Powder Packet
1. Use scissors to open the powder packet.
2. Push the edges of the packet to form a spout.
3. Pour out the content of the packet.
Proper Use of Dropper Bottle
1. Tap the dropper on the table several times.
Wipe the outside of the tip with a cloth.
2.
Always keep the dropper bottle in a vertical position
while dosing the reagent.
Proper Use of Syringe
1.
Push the plunger completely into the syringe and insert the tip into
the solution.
2. Pull the plunger up until the lower edge of the seal is exactly on the
mark for the desired volume.
3. Take out the syringe and clean the outside of the syringe tip, be sure
that no drops are hanging on the tip of the syringe.
4. Keep the syringe in a vertical position above the cuvette and then
push the plunger down into the syringe to deliver desired volume
into the cuvette.
Proper Use of Minipipette
1. Attach the pipette tip.
2. Press the button down to the first stop.
3. Immerse the pipette tip in the liquid approximately 2‑3 mm.
4. Slowly let the button move back to original position, wait 2 seconds.
5. Remove the pipette tip from the liquid.
6. To dispense the liquid, place pipette tip on container inside wall.
7. Slowly press button down to first stop.
8. Wait until all of the liquid has been dispensed.
9.
Press button down to second stop, this will allow any remaining liquid
to be dispensed.
6.4. CUVETTE PREPARATION
Proper mixing is very important for reproducibility of the measurements.
•
The mixing technique for each method is listed in the method procedure.
• In order to avoid reagent leaking and to obtain more accurate measurements, close
the cuvette first with the supplied HDPE plastic stopper and then the black cap.

19 Measurement Recommendations
Technique Icons Description
Invertion
For one inversion:
• Hold the cuvette in vertical position
• Turn the cuvette upside‑down and wait for all of the
solution to flow to the cap end
• Return the cuvette to the upright vertical position
• Wait for all of the solution to flow to the cuvette bottom
The correct speed for this technique is 10‑15 complete
inversions in 30 seconds.
Shake
Gentle shake Vigorous shake
6.5. MEASUREMENT RECOMMENDATIONS
General Guidelines
• Whenever the cuvette is placed into the measurement holder, it must be
dry outside and free of fingerprints, oil, or dirt. Wipe it thoroughly with
HI731318 microfiber cleaning cloth or a lint‑free wipe prior to insertion.
•
Shaking the cuvette can generate bubbles in the sample, causing higher
readings. To obtain accurate measurements, remove such bubbles by swirling
or by gently tapping the cuvette.
• Do not let the reacted sample stand too long after reagent has been added.
For best accuracy, respect the timings described in each method.
•
It is possible to take multiple readings in a row, but it is recommended to take a new zero reading for
each sample and to use the same cuvette for zeroing and measurement when possible.
•
Discard sample immediately after reading has been taken, or the glass might become permanently stained.
• All reaction times reported in this manual are at 25 °C (77 °F). In general, reaction time should be increased
for temperatures lower than 20 °C (68 °F), and decreased for temperatures higher than 25 °C (77 °F).

Battery Management 20
Method-Specific Guidelines
Marine Ammonia
• Prepare sample cuvette immediately after collecting sample from tank. Ammonia is volatile and will
dissipate if stored in a bottle before analysis, causing low measurements.
Marine Magnesium
• Keep the tips with their appropriate syringes during measurement.
• Measure liquids accurately by syringe by drawing up the plunger until the bottom seal
of the plunger is at the desired volume mark. Do NOT raise the liquid to the mark as
this will give a false high volume. An air gap between the plunger and liquid is normal.
See image at the right.
• Always use clean, dry cuvettes and syringes/tips.
• Rinse with deionized (RODI) water only; never rinse with tank water.
• Dry the cuvettes before use to prevent dilution.
• Clean the syringes and tips before storage.
6.6. BATTERY MANAGEMENT
The meter will perform an auto‑diagnostic test when it is powered on. During this test, the Hanna Instruments
®
logo will appear on the LCD. If the auto‑diagnostic test was successful, the meter is ready for use.
To conserve the battery, the meter turns off automatically after 15 minutes of inactivity.
If a zero reading has been done but not a Read, auto‑off time is increased to 30 minutes.
The battery icon on the LCD indicates the battery status:
Battery is full.
Battery is below 10 %. Replace batteries soon.
Battery is low. Replace batteries with new ones.
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2
3
4
5