Hanna Instruments HI97727 User manual

INSTRUCTION MANUAL
HI97727
Color of Water
Photometer

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the
instrument.
This manual will provide you with the necessary information for correct
use of the instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to
e‑mail us at [email protected] or view our worldwide contact
list at www.hannainst.com.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Dear
Customer,

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TABLE OF CONTENTS
1. PRELIMINARY EXAMINATION ........................................................................
2. SAFETY MEASURES......................................................................................
3. SPECIFICATIONS..........................................................................................
4. ABBREVIATIONS..........................................................................................
5. DESCRIPTION..............................................................................................
5.1. GENERAL DESCRIPTION AND INTENDED USE...........................................
5.2. FUNCTIONAL DESCRIPTION....................................................................
5.3. PRECISION AND ACCURACY....................................................................
5.4. PRINCIPLE OF OPERATION.....................................................................
5.5. OPTICAL SYSTEM ..................................................................................
6. GENERAL OPERATIONS.................................................................................
6.1. METER VALIDATION: CAL CHECK/CALIBRATION ........................................
6.2. GLP .....................................................................................................
6.3. LOGGING DATA/LOG RECALL...................................................................
6.4. GENERAL SETUP...................................................................................
6.5. CONTEXTUAL HELP................................................................................
6.6. BATTERY MANAGEMENT........................................................................
7. COLLECTING AND MEASURING SAMPLES........................................................
8. METHOD PROCEDURE ..................................................................................
9. ERROR DESCRIPTIONS .................................................................................
10. BATTERY REPLACEMENT .............................................................................
11. ACCESSORIES............................................................................................
CERTIFICATION................................................................................................
RECOMMENDATIONS FOR USERS......................................................................
WARRANTY .....................................................................................................
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1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully to
make sure that no damage has occurred during shipping. Notify your nearest Hanna
Customer Service Center if damage is observed.
Each HI97727C is supplied with:
• Sample cuvette (2 pcs.)
• Sample cuvette cap (2 pcs.)
• Plastic stopper (2 pcs.)
• A ZERO ‑ CAL Check Cuvette A
• HI97727B ‑ CAL Check Cuvette B for Color of Water
• Cloth for wiping cuvettes
• 1.5V AA Alkaline batteries
• Instruction manual
• Meter quality certificate
• CAL Check standard certificate
Each HI97727 is supplied with:
• Sample cuvette (2 pcs.)
• Sample cuvette cap (2 pcs.)
• Plastic stopper (2 pcs.)
• 1.5V AA Alkaline batteries
• Instruction manual
• Meter quality certificate
Note: Save all packing material until you are sure that the instrument works correctly.
Any damaged or defective item must be returned in its original packing material with the
supplied accessories.
2. SAFETY MEASURES
• The chemicals contained in the reagent kits may be hazardous if
improperly handled.
• Read the Safety Data Sheets (SDS) before performing tests.
• Safety equipment: Wear suitable eye protection and clothing when required, and
follow instructions carefully.
• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty
of water. If reagent contacts skin, rinse the affected area thoroughly with water.
Avoid breathing released vapors.
• Waste disposal: For proper disposal of reagent kits and reacted samples, contact
a licensed waste disposal provider.
PRELIMINARY EXAMINATIONSAFETY MEASURES

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Color of
Water
Range 0 to 500 PCU (Platinum Cobalt Units)
Resolution 1 PCU
Accuracy ±10 PCU ±5% of reading at 25 °C
Method
Adaptation of the Standard Methods
for the Examination of Water and
Wastewater, 18th edition, Colorimetric
Platinum Cobalt method
Measurement
System
Light source Light Emitting Diode
Bandpass filter 420 nm
Bandpass filter
bandwidth 8 nm
Bandpass filter
wavelength accuracy ±1.0 nm
Light detector Silicon photocell
Cuvette type Round 24.6 mm diameter (22 mm inside)
Additional
Specifications
Auto logging 50 readings
Display 128 x 64 pixel B/W LCD with backlight
Auto‑off After 15 minutes of inactivity
(30 minutes before a READ measurement)
Battery type Alkaline 1.5 V AA (3 pcs.)
Battery life > 800 measurements (without backlight)
Environment 0 to 50 °C (32 to 122 °F);
0 to 100% RH, non‑serviceable
Dimensions 142.5 x 102.5 x 50.5 mm (5.6 x 4.0 x 2.0")
Weight (with batteries) 380 g (13.4 oz.)
Case ingress protection
rating IP67, floating case
3. SPECIFICATIONS
SPECIFICATIONS
4. ABBREVIATIONS
mg/L milligrams per liter (ppm)
mL milliliter
°C degree Celsius
°F degree Fahrenheit
LED Light Emitting Diode
HDPE High Density Polyethylene
GLP Good Laboratory Practice
PCU Platinum Cobalt Units
DIW Deionized Water
NIST National Institute of Standards and Technology
ABBREVIATIONS

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DESCRIPTION
5. DESCRIPTION
5.1. GENERAL DESCRIPTION AND INTENDED USE
The HI97727 is an auto‑diagnostic portable meter that benefits from Hanna’s years
of experience as a manufacturer of analytical instruments. It has an advanced optical
system that uses a Light Emitting Diode (LED) and a narrow band interference filter
that allows for accurate and repeatable readings.
The optical system is sealed from outside dust, dirt and water. The meter uses an
exclusive positive‑locking system to ensure that the cuvettes are placed into the holder
in the same position every time.
With the CAL Check functionality, users are able to validate the performance of the
instrument at any time and apply a user calibration (if necessary). Hanna's CAL Check
cuvettes are made with NIST traceable standards.
The built in Tutorial mode guides users step‑by‑step through the measurement process. It
includes all steps required for sample preparation, the required reagents and quantities.
The HI97727 meter measures color of water samples from 0 to 500 PCU. The method is
an adaptation of the Standard Methods for the Examination of Water and Wastewater,
18th edition, Colorimetric Platinum Cobalt method.
By comparison with known color of platinum cobalt chloride standards method is used
to determine “apparent color” of water (color from suspended and dissolved components
measured using un‑filtered samples) and “true color” (measured using filtered samples
after removal of slightly turbidity).
The HI97727 photometer is a compact and versatile meter suitable for field or bench
measurements, featuring a:
• Sophisticated optical system
• Meter validation using certified CAL Check cuvettes
• Tutorial Mode guides the user step‑by‑step
• Auto logging
• Waterproof IP67, floating case
• GLP Features

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Keypad Description
The keypad contains 3 direct keys and 3 functional keys with the following functions:
Press the functional keys to perform the function displayed above them on the LCD.
Press and hold to power off/on. Press briefly to return to the previous screen.
Press to access the menu screen.
Press to display the context‑sensitive help menu.
DESCRIPTION
1) ON/OFF power button 3) Liquid Crystal Display (LCD) 5)Indexing mark
2) Keypad 4) Cuvette holder 6) Battery cover
5.2. FUNCTIONAL DESCRIPTION

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DESCRIPTION
5.4. PRINCIPLE OF OPERATION
Absorption of light is a typical phenomenon of interaction between electromagnetic
radiation and matter. When a light beam crosses a substance, some of the radiation
may be absorbed by atoms, molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical
path length through the matter and on the physical‑chemical characteristics of the
substance according to the Lambert‑Beer Law:
‑log I/Io= elc d
or
A = elc d
Io= intensity of incident light beam
I = intensity of light beam after absorption
el = molar extinction coefficient at wavelength l
c = molar concentration of the substance
d = optical path through the substance
Therefore, the concentration "c" can be calculated from the absorbance of the substance
as the other factors are constant.
Photometric chemical analysis is based on specific chemical reactions between a
sample and reagent to produce a light‑absorbing compound.
5.3. PRECISION AND ACCURACY
Precision is how closely repeated
measurements are to one another.
Precision is usually expressed as
standard deviation (SD).
Accuracy is defined as the
closeness of a test result to the
true value.
Although good precision suggests
good accuracy, precise results can
be inaccurate. The figure explains
these definitions.
For each method, the accuracy is expressed in the related measurement section.

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DESCRIPTION
Instrument Block Diagram
The internal reference system (reference detector) of the HI97727 photometer
compensates for any drifts due to power fluctuations or ambient temperature changes,
providing a stable source of light for your blank (zero) measurement and sample
measurement.
LED light sources offer superior performance compared to tungsten lamps. LEDs have
a much higher luminous efficiency, providing more light while using less power. They
also produce little heat, which could otherwise affect electronic stability. LEDs are
available in a wide array of wavelengths, whereas tungsten lamps have poor blue/
violet light output.
Improved optical filters ensure greater wavelength accuracy and allow a brighter,
stronger signal to be received. The end result is higher measurement stability and less
wavelength error.
A focusing lens collects all of the light that exits the cuvette, eliminating errors from
cuvette imperfections and scratches, eliminating the need to index the cuvette.
5.5. OPTICAL SYSTEM

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GENERAL OPERATIONS
To perform a CAL Check:
1. Press CAL Check key from measurement mode.
The “Not Available“ message or the date/time
and status of the last CAL Check will be displayed
on the screen.
2. Press the Check key to start a new CAL Check.
Press the key at any time to abort the
validation process.
3. Use the keys to enter the certificate value
of the calibration standard found on the CAL Check
Standard Certificate. Press Next to continue.
Note: This value will be saved in the instrument for future validation. If a new
set of calibration standards is obtained please update the certificate value.
4. Insert the A ZERO CAL Check Cuvette A then press
Next to continue. The “Please Wait...“message
will be displayed during the measurement.
5. Insertthe HI97727B CALCheck CuvetteB then press
Next to continue. The “Please Wait...“message
will be displayed during the measurement.
6.1. METER VALIDATION: CAL CHECK / CALIBRATION
Validation of the HI97727 involves verifying the concentration of the certified CAL
Check standards. The CAL Check screen guides the user step‑by‑step through the
validation process and user calibration (if necessary).
WARNING: Do not use any solutions/standards other than the HANNA®CAL Check
Standards. For accurate validation and calibration results, please perform these at
room temperature (18 to 25 °C; 64.5 to 77.0 °F).
Note: CAL Check Standards will not read the specified value in measurement mode.
Protect the CAL Check cuvettes from direct sunlight by keeping them in the original
packing. Store between +5 °C and +30 °C (41 - 86 °F), do not freeze.
6. GENERAL OPERATIONS

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‑ “PASSED“: The measured value is within the accuracy
specification, no user calibration is required.
‑ “OUT OF SPECIFICATION“ and the Calibration
key is available: The measured value is near the expected value. To update the
user calibration press Calibrate. Press Accept to confirm or Cancel to return to the
previous screen.
GENERAL OPERATIONS
‑ “OUT OF SPECIFICATION“: A user calibration is
not allowed, the measured value is outside of the tol‑
erance window. Check the certified value, expiration
date and clean the outside of the cuvette. Repeat
the CAL Check procedure. If this error continues contact your nearest Hanna Customer
Service Center.
6.2. GLP
Press the key to enter the menu. Use the 
keys to select GLP and press Select. Good Laboratory
Practice (GLP) shows the date and time of the last
user calibration (if available) or factory calibration. To
erase the last user calibration and to clear the CAL Check press Clear and follow the
prompts. Press Yes to erase and return to the factory calibration data or No to exit the
clear procedure.
6. When the CAL Check is complete the display will
show one of the following messages and the value
obtained during the measurement:

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6.3. LOGGING DATA/LOG RECALL
The instrument features a data autolog function to help users keep track of all
measurements. Every time a measurement is made the data is automatically saved.
The data log can hold 50 individual measurements. When the 50 measurements are
full and there is no deleted data, the meter will rewrite the oldest log.
Viewing and deleting the data is possible using the Log Recall menu.
Press the key to enter the menu. Use the functional keys to select Log
Recall and press Select.
Use the functional keys to highlight a log and press Info to view additional
information about the log. From this screen the Next and Previous keys can be used
to view other logs.
Use the Delete key to erase logged data. After pressing Delete a prompt on display
is asking for confirmation.
GENERAL OPERATIONS
Press No or the key to return to the previous screen.
Press Yes to delete selected log.
Press Del All to erase all the logged data.
If Del All is pressed follow the prompt to confirm.
Press Yes to delete all logged data, No or the key to return to the log recall.

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GENERAL OPERATIONS
Backlight
Values: 0 to 100 %
Press the Modify key to access the backlight intensity.
Use the keys to increase or decrease the value.
Press the Accept key to confirm or the key to
return to the Setup menu without saving the new value.
Date / Time
Press the Modify key to change the date/time.
Press the keys to highlight the value to be
modified (year, month, day, hour, minute or second).
Press Edit to modify the highlighted value. Use the
keys to change the value.
Time Format
Option: AM/PM or 24-hour
Press the functional key to select the desired time
format.
Contrast
Values: 0 to 100 %
Press the Modify key to change the display’s contrast.
Use the keys to increase or decrease the value.
Press the Accept key to confirm the value or the
key to return to the Setup menu without saving the
new value.
6.4. GENERAL SETUP
Press the key to enter the menu. Use the keys to select Setup and press
Select. Use the keys to highlight desired option.
Press the Accept key to confirm or the key to
return to the previous screen.

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Language
Press the Modify key to change the language. Use the
keys to select the desired language.
Press Accept to choose one of the languages installed.
Date Format
Press the Modify key to change the date format.
Use the keys to select the desired format.
Press the Accept key to confirm or the key to
return to the Setup menu without saving the new
format.
Decimal Separator
Option: Comma ( , ) or Period ( . )
Press the functional key to select the desired decimal
separator. The decimal separator is used on the
measurement screen.
Beeper
Option: Enable or Disable
When enabled, a short beep is heard every time a
key is pressed. A long beep alert sounds when the
pressed key is not active or an error is detected. Press
the functional key to enable/disable the beeper.
Tutorial
Option: Enable or Disable
When enabled, the user will be guided step‑by‑step
through the measurement procedure.
Meter Information
Press the Select key to view the model, serial number,
firmware version and selected language. Press the
key to return to the Setup menu.
GENERAL OPERATIONS

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GENERAL OPERATIONS
Restore factory settings
Press the Select key to reset to factory settings.
Press Accept to confirm or Cancel to exit without
restoring the factory settings.
6.6. BATTERY MANAGEMENT
The meter will perform an auto‑diagnostic test when it is powered on. During this test,
the HANNA®logo will appear on the LCD. After 5 seconds, if the test was successful,
the last method selected will appear on the display. The battery icon on the LCD will
indicate the battery status:
‑ battery full
‑ battery below 10%, replace the batteries soon
‑ battery is low, replace the batteries with new ones
To conserve battery, the meter will turn off automatically after 15 minutes of inactivity.
If a zero reading has been done but not a read, auto‑off time is increased to 30 minutes.
6.5. CONTEXTUAL HELP
The HI97727 offers an interactive contextual help
mode that assists the user at any time.
To access the help screen press the key.
The instrument will display additional information
related to the current screen. To read all the available information, scroll the text using
the keys.
To exit help mode press the or the key and the meter will return to the
previous screen.

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COLLECTING AND MEASURING SAMPLES
7. COLLECTING AND MEASURING SAMPLES
In order to avoid leaking and to obtain more accurate measurements, close
the cuvette first with the supplied HDPE plastic stopper and then the
black cap.
Whenever the cuvette is placed into the measurement holder, it must be
dry outside and free of fingerprints, oil or dirt. Wipe it thoroughly with
HI731318 or a lint‑free cloth prior to insertion.
Shaking the cuvette can generate bubbles in the sample, causing higher readings. To
obtain accurate measurements, remove such bubbles by swirling or by gently tapping
the cuvette.
It is possible to take multiple readings in a row, but it is recommended to take a new
zero reading for each sample and to use the same cuvette for
zeroing and measurement when possible.
Discard the sample immediately after the reading is taken, or
the glass might become permanently stained.
For reproducible results it is adviced that measurements are
taken close to 25 °C (77 °F).

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METHOD PROCEDURE
8. METHOD PROCEDURE
Note: If tutorial mode is disabled follow the measurement procedure below. If the
tutorial mode is enabled, press Measure and follow the messages on the screen.
• Fill the first cuvette (#1) up to the mark with
deionized water and replace the cap.
• Insert the cuvette (#1) into the holder and check the
notch alignment.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and
ready for measurement.
• Remove the cuvette.
10 mL
#1
• Fill the second cuvette (#2) up to the mark with
unfiltered sample and replace the cap. This is the
apparent color.
10 mL
#2
#1

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METHOD PROCEDURE
• Filter 10 mL of sample through a filter with a 0.45 µm
membrane into the third cuvette (#3), up to the 10 mL
mark and replace the plastic stopper and the cap. This
is the true color.
• Insert the apparent color cuvette (#2) into the holder
and check the notch alignment.
• Press Read to start the reading. The meter displays the value of apparent color in
Platinum Cobalt Units (PCU).
#2
#3
•Press Read to start the reading. The meter displays the true color in Platinum
Cobalt Units (PCU).
• Remove the apparent color cuvette(#2) from the
instrument, insert the true color cuvette (#3) into
the holder and check the notch alignment.
#3
10 mL

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ERROR DESCRIPTIONS
The instrument shows clear warning messages when erroneous conditions appear and when
measured values are outside the expected range. These messages are described below.
No Light: The light source is not functioning properly.
Light Leak: There is an excess amount of ambient light
reaching the detector.
Inverted Cuvette: The sample and the zero cuvettes
are inverted.
Light Low: The instrument cannot adjust the light
level. Please check that the sample does not contain
any debris.
Light High: There is too much light to perform a
measurement. Please check the preparation of the
zero cuvette.
9. ERROR DESCRIPTIONS
Ambient temperature out of limits: The meter is too
hot or too cold for an accurate measurement. Allow
the meter to reach 10 °C to 40 °C (50 °F to 104 °F)
before performing a measurement.
Ambient temperature changed: The temperature
of the meter has changed significantly since the
zero measurement has been performed. A zero
measurement must be performed again.
Out of range: The measured value is outside the limits
of the method.
Battery Low: Battery is low, replace the batteries with
new ones.

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To replace the instrument’s batteries, follow these steps:
• Turn the instrument off by pressing and holding the key.
• Remove the battery cover by turning it counterclockwise.
• Remove the old batteries, replace them with three new 1.5V AA batteries.
• Replace the battery cover, turn it clockwise to close.
10. BATTERY REPLACEMENT
BATTERY REPLACEMENT