Hanna Instruments HI97769 User manual

Hanna Instruments Inc., 584 Park East Drive, Woonsocket, RI 02895 USA
www.hannainst.com
INSTRUCTION MANUAL
HI97769
Anionic Surfactants
Photometer

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner,
Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Hanna Instruments reserves the right to modify the design, construction, or appearance of its products without advance notice.
TABLE OF CONTENTS
1. Preliminary Examination ...............................3
2. Safety Measures..............................................3
3. Abbreviations .................................................4
4. Specifications..................................................4
5. Description......................................................5
5.1. General Description & Intended Use .................... 5
5.2. Functional Description........................................ 6
5.3. Precision & Accuracy...........................................7
5.4. Principle of Operation.........................................7
5.5. Optical System...................................................7
6. General Operations ........................................8
6.1. Meter Validation: CAL Check™ & Calibration....... 8
6.2. GLP.................................................................10
6.3. Logging Data & Log Recall ...............................10
6.4. General Setup.................................................. 11
6.5. Reagents & Accessories ....................................13
6.6. Contextual Help ...............................................13
6.7. Battery Management ....................................... 13
7. Photometer...................................................14
7.1. Proper Use of Dropper Bottle.............................14
7.2. Glass Vial & Cuvette Preparation....................... 14
8. Method Procedure ........................................15
9. Warning & Error Descriptions.......................18
10. Battery Replacement...................................19
11. Accessories ..................................................19
11.1. Reagent Sets.................................................19
11.2. Other Accessories ...........................................19
Certification .......................................................20
Recommendations for Users..............................20
Warranty............................................................20
Dear Customer,
Thank you for choosing a Hanna Instruments®product.
Please read this instruction manual carefully before using this instrument as it provides the necessary
information for correct use of this instrument as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at [email protected].
Visit www.hannainst.com for more information about Hanna Instruments and our products.

3 Preliminary Examination
1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully. For further assistance,
please contact your local Hanna Instruments office®or email us at [email protected].
Each HI97769C is delivered in a rugged carrying case and is supplied with:
• Sample cuvette (2 pcs.)
• Sample cuvette cap (2 pcs.)
• Plastic stopper (2 pcs.)
• A ZERO ‑ CAL Check™Cuvette A
• HI97769B ‑ CAL Check Cuvette B for Anionic Surfactants
• Cloth for wiping cuvettes
• 1.5V AA Alkaline battery (3 pcs.)
• CAL Check standard certificate
• Quick reference guide with instructions for manual download and instrument quality certificate
Each HI97769 is delivered in a cardboard box and is supplied with:
• Sample cuvette (2 pcs.)
• Sample cuvette cap (2 pcs.)
• Plastic stopper (2 pcs.)
• 1.5V AA Alkaline battery (3 pcs.)
• Quick reference guide with instructions for manual download and instrument quality certificate
Note: Save all packing material until you are sure that the instrument works correctly. Any damaged or defective
item must be returned in its original packing material with the supplied accessories.
2. SAFETY MEASURES
• The chemicals contained in the reagent kits may be hazardous if improperly handled.
• Read the Safety Data Sheets (SDS) before performing tests.
•
Safety equipment: Wear suitable eye protection and clothing when required, and follow instructions
carefully.
•
Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of water.
If reagent contacts skin, rinse the affected area thoroughly with water. Avoid breathing released
vapors.
•
Waste disposal: For proper disposal of reagent kits and reacted samples, contact a licensed waste
disposal provider.

Abbreviations 4
3. ABBREVIATIONS
mg/L milligrams per liter (ppm)
mL milliliter
°C degree Celsius
°F degree Fahrenheit
EPA US Environmental Protection Agency
GLP Good Laboratory Practice
HDPE High Density Polyethylene
LED Light Emitting Diode
NIST National Institute of Standards and Technology
4. SPECIFICATIONS
Anionic Surfactants
Range 0.00 to 3.50 mg/L (as SDBS)
Resolution 0.01 mg/L
Accuracy ±0.04 mg/L ±3% of reading at 25 °C
Method
Adaptation of the US EPA Method 425.1 and Standard Methods for
the Examination of Water & Wastewater, 20th Edition, 5540C, Anionic
Surfactants as MBAS
Measurement System
Light source Light Emitting Diode
Bandpass filter 610 nm
Bandpass filter bandwidth 8 nm
Bandpass filter wavelength accuracy ±1.0 nm
Light detector Silicon photocell
Cuvette type Round 24.6 mm diameter (22 mm inside)
Additional Specifications
Auto logging 50 readings
Display 128×64 pixel B/W LCD with backlight
Auto‑off After 15 minutes of inactivity (30 minutes before a READ measurement)
Battery type 1.5 V AA Alkaline (3 pcs.)
Battery life >800 measurements (without backlight)
Environment 0 to 50 °C (32 to 122 °F); 0 to 100% RH, non‑serviceable
Dimensions 142.5×102.5×50.5 mm (5.6×4.0×2.0")
Weight (with batteries) 380 g (13.4 oz.)
Case ingress protection rating IP67, floating case

5 Description
5. DESCRIPTION
5.1. GENERAL DESCRIPTION & INTENDED USE
The HI97769 is an auto‑diagnostic portable photometer that benefits from Hanna’s®years of experience as
a manufacturer of analytical instruments. It has an advanced optical system that uses a Light Emitting Diode
(LED) and a narrow band interference filter that allows for accurate and repeatable readings.
The optical system is sealed from outside dust, dirt and water. The meter uses an exclusive positive‑locking
system to ensure that the cuvettes are placed into the holder in the same position every time.
With the CAL Check™ functionality, users are able to validate the performance of the instrument at any time
and apply a user calibration (if necessary). Hanna Instruments®CAL Check cuvettes are made with NIST
traceable standards.
The built‑in tutorial mode guides users step‑by‑step through the measurement process. It includes all steps
required for sample preparation, the required reagents and quantities.
The HI97769 meter measures the anionic surfactants content in water samples in the 0.00 to 3.50 mg/L (ppm)
range. The method is an adaptation of the US EPA Method 425.1 and Standard Methods for the Examination
of Water & Wastewater, 20th Edition, 5540C, Anionic Surfactants as MBAS.
The HI97769 photometer is a compact and versatile meter suitable for field or bench measurements, featuring a:
• Sophisticated optical system
• Meter validation using certified CAL Check cuvettes
• Tutorial mode guides the user step‑by‑step
• Auto logging
• Waterproof IP67, floating case
• GLP features

Description 6
5.2. FUNCTIONAL DESCRIPTION
SN: XXXXXXXXXXXX
HI97769
1. ON/OFF power button
2. Keypad
3. Liquid Crystal Display (LCD)
4. Cuvette holder
5. Indexing mark
6. Battery cover
Keypad Description
The keypad contains 3 direct keys and 3 functional keys with the following functions:
Press the functional key to perform the function displayed above it on the LCD.
Press and hold to power off/on. Press briefly to return to the previous screen.
Press to access the menu screen.
Press to display the context‑sensitive help menu.

7 Description
5.3. PRECISION & ACCURACY
Precision is how closely repeated measurements are to one another. Precision is usually expressed as standard
deviation (SD).
Accuracy is defined as the closeness of a test result to the true value.
Although good precision suggests good accuracy, precise results can be inaccurate. The figure explains these
definitions.
5.4. PRINCIPLE OF OPERATION
Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and matter.
When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal
lattices. Photometric chemical analysis is based on specific chemical reactions between a sample and reagent
to produce a light‑absorbing compound.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the
matter and on the physical‑chemical characteristics of the substance according to the Lambert‑Beer Law. If all
other factors are constant, the concentration "c" can be calculated form the absorbance of the substance.
Lambert‑Beer Law:
– log I/Io= elc d
or
A = elc d
Io= intensity of incident light beam
I= intensity of light beam after absorption
el= molar extinction coefficient at wavelength l
c = molar concentration of the substance
d = optical path through the substance
5.5. OPTICAL SYSTEM
The internal reference system (reference detector) of the HI97769 photometer compensates for any drifts due
to power fluctuations or ambient temperature changes, providing a stable source of light for your blank (zero)
measurement and sample measurement.
LED light sources offer superior performance compared to tungsten lamps. LEDs have a much higher luminous
efficiency, providing more light while using less power. They also produce little heat, which could otherwise
affect electronic stability. LEDs are available in a wide array of wavelengths, whereas tungsten lamps have
poor blue/violet light output.
Improved optical filters ensure greater wavelength accuracy and allow a brighter, stronger signal to be received.
The end result is higher measurement stability and less wavelength error.

General Operations 8
A focusing lens collects all of the light that exits the cuvette, eliminating errors from cuvette imperfections and
scratches, eliminating the need to index the cuvette.
Instrument Block Diagram
6. GENERAL OPERATIONS
6.1. METER VALIDATION: CAL CHECK™ & CALIBRATION
Validation of the HI97769 involves verifying the concentration of the certified CAL Check standards. The CAL Check
screen guides the user step‑by‑step through the validation process and user calibration (if necessary).
WARNING: Do not use any solutions or standards other than the Hanna Instruments
®
CAL Check Standards.
For accurate validation and calibration results, please perform these at room temperature, 18 to 25 ° C
(64.5 to 77.0 ° F).
Note: CAL Check Standards will not read the specified value in measurement mode. Protect the CAL Check
cuvettes from direct sunlight by keeping them in the original packing. Store between 5 and 30 ° C (41 to 86 ° F),
do not freeze.
To perform a CAL Check:
1. Press CAL Check from measurement mode.
The “Not Available“message or the date, time and status of the last CAL Check will be displayed on the
screen.
2. Press Check to start a new CAL Check. Press the key at any time to abort the validation process.

9 General Operations
3. Use the functional keys to enter the certificate value of the calibration standard found on the CAL Check™
Standard Certificate. Press Next to continue.
Note: This value will be saved in the instrument for future validation. If a new set of calibration standards
is obtained please update the certificate value.
4. Insert the A ZERO CAL Check Cuvette A then press Next to continue. The “Please wait...“message will be
displayed during the measurement.
5. Insert the HI97769B CAL Check Cuvette B then press Next to continue. The “Please wait...“message will
be displayed during the measurement.
6. When the CAL Check is complete the display will show one of the following messages and the value
obtained during the measurement:
• “PASSED“: The measured value is within the accuracy specification, no user calibration is required.
• “OUT OF SPECIFICATION“ and Calibrate is available: The measured value is near the expected
value. To update the user calibration press Calibrate. Press Accept to confirm or Cancel to return to
the previous screen.
• “OUT OF SPECIFICATION“: A user calibration is not allowed, the measured value is outside of the tolerance
window. Check the certified value, expiration date and clean the outside of the cuvette. Repeat the CAL Check
procedure. If this error continues contact your nearest Hanna Instruments®Customer Service Center.

General Operations 10
6.2. GLP
Press the key to enter the menu. Use the functional keys to select GLP and press Select. Good Laboratory
Practice (GLP) shows the date and time of the last user calibration (if available) or factory calibration. To erase
the last user calibration and to clear the CAL Check™press Clear and follow the prompts. Press Yes to erase
and return to the factory calibration data or No to exit the clear procedure.
6.3. LOGGING DATA & LOG RECALL
The instrument features a data autolog function to help users keep track of all measurements. Every time a
measurement is made the data is automatically saved. The data log can hold 50 individual measurements.
When the data log is full (50 data points) the meter will rewrite the oldest data point.
Viewing and deleting the data is possible using the Log Recall menu.
Press the key to enter the menu. Use the functional keys to select Log Recall and press Select.
Use the functional keys to highlight a log and press Info to view additional information about the log. From
this screen Next and Previous can be used to view other logs.
Press Delete to erase logged data. After pressing Delete a prompt on display is asking for confirmation.
Press No or the key to return to the previous screen.
Press Yes to delete selected log.
Press Del All to erase all the logged data. If Del All is pressed follow the prompt to confirm. Press Yes to delete
all logged data, No or the key to return to the log recall.