Hanna Instruments HI83303 User manual

INSTRUCTION MANUAL
HI83303
Multiparameter Photometer
for Aquaculture

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the
copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using this instrument.
This manual will provide you with the necessary information for correct use of this
instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
[email protected] or view our worldwide contact list at www.hannainst.com.
Dear
Customer,

3
TABLE OF CONTENTS
TABLE OF CONTENTS
1. PRELIMINARY EXAMINATION ............................................................................................... 5
2. SAFETY MEASURES............................................................................................................. 5
3. SPECIFICATIONS................................................................................................................. 6
4. ABBREVIATIONS ................................................................................................................ 7
5. DESCRIPTION..................................................................................................................... 8
5.1. GENERAL DESCRIPTION & INTENDED USE .................................................................. 8
5.2. PRECISION & ACCURACY............................................................................................ 8
5.3. FUNCTIONAL DESCRIPTION........................................................................................ 9
5.4. PRINCIPLE OF OPERATION....................................................................................... 10
5.5. OPTICAL SYSTEM..................................................................................................... 11
6. GENERAL OPERATIONS...................................................................................................... 12
6.1. POWER CONNECTION & BATTERY MANAGEMENT ....................................................... 12
6.2. MODE SELECTION ................................................................................................... 12
6.3. GENERAL SETUP ..................................................................................................... 13
6.4. CONTEXTUAL HELP.................................................................................................. 16
7. LOGGING DATA & DATA MANAGEMENT................................................................................ 17
7.1. LOGGING DATA ....................................................................................................... 17
7.2. ADDING SAMPLE & USER NAMES TO LOG DATA......................................................... 17
7.3. DATA MANAGEMENT................................................................................................ 18
8. PHOTOMETER MODE......................................................................................................... 19
8.1. METHOD SELECTION ............................................................................................... 19
8.2. COLLECTING & MEASURING SAMPLES AND REAGENTS................................................ 19
8.3. CUVETTE PREPARATION........................................................................................... 20
8.4. TIMERS & MEASUREMENT FUNCTIONS..................................................................... 22
8.5. CHEMICAL FORMULA & UNIT CONVERSION............................................................... 22
8.6. METER VALIDATION & CAL CHECK............................................................................. 23
8.7. ABSORBANCE MEASUREMENTS................................................................................ 24
9. PROBE MODE................................................................................................................... 25
9.1. pH MEASUREMENT................................................................................................. 25
9.2. pH CALIBRATION..................................................................................................... 26
9.3. pH MESSAGES & WARNINGS ................................................................................... 27
9.4. pH GLP .................................................................................................................. 28
9.5. pH ELECTRODE CONDITIONING & MAINTENANCE....................................................... 29

4
TABLE OF CONTENTS
10. METHOD PROCEDURES ................................................................................................... 31
10.1. ALKALINITY........................................................................................................... 31
10.2. ALKALINITY, MARINE............................................................................................. 33
10.3. AMMONIA LOW RANGE.......................................................................................... 35
10.4. AMMONIA MEDIUM RANGE ................................................................................... 38
10.5. AMMONIA HIGH RANGE ........................................................................................ 41
10.6. CALCIUM.............................................................................................................. 44
10.7. CALCIUM, MARINE ................................................................................................ 47
10.8. CHLORINE, FREE................................................................................................... 49
10.9. CHLORINE, TOTAL ................................................................................................. 52
10.10. COPPER LOW RANGE........................................................................................... 56
10.11. COPPER HIGH RANGE.......................................................................................... 58
10.12. NITRATE............................................................................................................. 60
10.13. NITRITE, MARINE ULTRA LOW RANGE................................................................... 63
10.14. NITRITE LOW RANGE........................................................................................... 65
10.15. NITRITE HIGH RANGE.......................................................................................... 67
10.16. OXYGEN, DISSOLVED .......................................................................................... 69
10.17. pH..................................................................................................................... 72
10.18. PHOSPHATE, MARINE ULTRA LOW RANGE............................................................. 74
10.19. PHOSPHATE LOW RANGE..................................................................................... 76
10.20. PHOSPHATE HIGH RANGE.................................................................................... 78
11. WARNINGS & ERRORS.................................................................................................... 81
12. STANDARD METHODS ..................................................................................................... 84
13. ACCESSORIES................................................................................................................. 85
13.1. REAGENT SETS...................................................................................................... 85
13.2. pH ELECTRODES ................................................................................................... 87
13.3. pH SOLUTIONS..................................................................................................... 88
13.4. OTHER ACCESSORIES ............................................................................................ 90
CERTIFICATION...................................................................................................................... 92
RECOMMENDATIONS FOR USERS............................................................................................ 92
WARRANTY ........................................................................................................................... 93

5
PRELIMINARY EXAMINATION
1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully. For further
assistance, please contact your local Hanna Instruments Office or email us at [email protected].
Each HI83303 is delivered in a rugged carrying case and is supplied with:
• Sample cuvette (4 pcs.)
• Sample cuvette cap (4 pcs.)
• Cloth for wiping cuvettes
• Scissors
• USB cable
• 5 Vdc power adapter
• 60 mL glass bottle
• Instrument quality certificate
• Instruction manual
Note: Save all packing material until you are sure that the instrument works correctly. Any
damaged or defective item must be returned in its original packing material with the supplied
accessories.
2. SAFETY MEASURES
• The chemicals contained in the reagent kits may be hazardous if improperly handled.
• Read the Safety Data Sheets (SDS) before performing tests.
• Safety equipment: Wear suitable eye protection and clothing when required and follow
instructions carefully.
• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of
water. If reagent contacts skin, rinse the affected area thoroughly with water. Avoid
breathing released vapors.
• Waste disposal: For proper disposal of reagent kits and reacted samples, contact a
licensed waste disposal provider.
SAFETY MEASURES

6
SPECIFICATIONS
3. SPECIFICATIONS
Measurement Channels 5 x optical channels
1 x digital electrode channel (pH measurement)
Photometer
Range 0.000 to 4.000 Abs
Resolution 0.001 Abs
Accuracy ±0.003 Abs @ 1.000 Abs
Light source Light Emitting Diode
Bandpass filter bandwidth 8 nm
Bandpass filter wavelength accuracy ±1.0 nm
Light detector Silicon photocell
Cuvette types Round, 24.6 mm diameter
Number of methods 25
Probe
Range -2.00 to 16.00 pH (± 1000.0 mV)*
Resolution 0.01 pH (0.1 mV)
Accuracy ±0.01 pH (±0.2 mV) @ 25 °C / 77 °F
Temperature compensation ATC, -5.0 to 100.0 °C (23.0 to 212.0 °F)*
Calibration two-point, from five available buffers
(4.01, 6.86, 7.01, 9.18, 10.01 pH)
Electrode Intelligent pH / temperature electrode
Temperature
Range -20.0 to 120.0 °C (-4.0 to 248.0 °F)
Resolution 0.1 °C (0.1 °F)
Accuracy ±0.5 °C @ 25 °C (±0.9 °F @ 77 °F)
Additional
Specifications
Logging 1000 readings (mixed photometer and electrode)
Display 128 x 64 pixel B/W LCD with backlight
USB-A (Host) functions Mass-storage host
USB-B (Device) functions Power input, mass-storage device
Battery life > 500 photometer measurements or 50 hours
of continuous pH measurement
Power supply
5 Vdc USB 2.0 power adapter / type micro-B
connector
3.7 Vdc Li-polymer rechargeable battery,
non-serviceable
Environment 0 to 50 °C (32 to 122 °F)
0 to 95% RH, non-serviceable
Dimensions 206 x 177 x 97 mm (8.1 x 7.0 x 3.8")
Weight 1.0 kg (2.2 lbs.)
*Limits will be reduced to actual probe / sensor limits.

7
ABBREVIATIONS
4. ABBREVIATIONS
Abs Absorbance
GLP Good Laboratory Practice
NIST National Institute of Standards and Technology
EPA US Environmental Protection Agency
g/L grams per liter (ppt)
µg/L micrograms per liter (ppb)
mg/L milligrams per liter (ppm)
mL milliliter
HR High Range
LR Low Range
MR Medium Range
ULR Ultra Low Range

8
DESCRIPTION
5. DESCRIPTION
5.1. GENERAL DESCRIPTION & INTENDED USE
HI83303 multiparameter photometer is a compact and versatile meter with two measurement modes,
Photometer and Probe. Photometer mode includes a CAL Check™ feature and 25 different methods
that cover a wide variety of applications, making it ideal for both benchtop and portable operations.
With the CAL Check feature users are able to validate the performance of the instrument and apply a
user calibration (if necessary). Hanna Instruments CAL Check cuvettes are made with NIST traceable
standards. Probe mode uses a digital pH probe with a one or two-point calibration.
• Digital electrode input for pH measurements
• Certified CAL Check cuvettes to confirm meter functionality
• Dual purpose micro-USB flash drive
• Lithium polymer rechargeable battery
• Auto-off
• Absorbance mode
• User and sample name entry
• GLP features
5.2. PRECISION & ACCURACY
Precision is how closely repeated measurements are to one another. Precision is usually expressed as
standard deviation. Accuracy is defined as the closeness of a test result to the true value. Although
good precision suggests good accuracy, precise results can be inaccurate. The figure explains these
definitions. For each method, the accuracy is expressed in the related measurement section.

9
DESCRIPTION
5.3. FUNCTIONAL DESCRIPTION
1. Splash-proof keypad
2. Liquid Crystal Display (LCD)
3. Indexing mark
4. Protective port covers
5. Light-blocking cover panel
6. Cuvette holder
7. ON/OFF power button
8. 3.5 mm TRRS (jack) input for digital electrodes
9. Standard USB host connector for data transfer to a USB flash drive
10. Micro-USB device connector for power or PC interface

10
DESCRIPTION
Keypad Description
The keypad contains 12 direct keys and 3 functional keys with the following functions:
Press the functional key to perform the function displayed above it on the LCD.
Press to access the list of photometer methods.
Press to move up in a menu or a help screen, to increment a set value or to access second
level functions.
Press to toggle between photometer and probe (pH electrode) mode.
Press to move left in a menu or to decrement a set value.
Press to move down in a menu or a help screen, to decrement a set value or to access second
level functions.
Press to move right in a menu or to increment a set value.
Press to access the setup screen.
Press to log the current reading.
Press to review saved logs.
Press to exit the current screen.
Press to display the help screen.
ON/OFF power button
5.4. PRINCIPLE OF OPERATION
Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms,
molecules or crystal lattices. Photometric chemical analysis is based on specific chemical reactions
between a sample and reagent to produce a light-absorbing compound.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical-chemical characteristics of the substance according to the
Lambert-Beer Law. If all other factors are constant, the concentration "c" can be calculated from the
absorbance of the substance.

11
DESCRIPTION
Lambert Beer Law:
-log I/Io= elc d
or
A = elc d
Io= intensity of incident light beam
I= intensity of light beam after absorption
el= molar extinction coefficient at wavelength l
c = molar concentration of the substance
d = optical path through the substance
5.5. OPTICAL SYSTEM
Instrument Block Diagram
The internal reference system (reference detector) of the HI83303 photometer compensates for any
drifts due to power fluctuations or ambient temperature changes, providing a stable source of light for
your blank (zero) measurement and sample measurement.
LED light sources offer superior performance compared to tungsten lamps. LEDs have a much higher
luminous efficiency, providing more light while using less power. They also produce little heat, which
could otherwise affect electronic stability. LEDs are available in a wide array of wavelengths, whereas
tungsten lamps have poor blue / violet light output.
Improved optical filters ensure greater wavelength accuracy and allow a brighter, stronger signal to be
received. The end result is higher measurement stability and less wavelength error.
A focusing lens collects all of the light that exits the cuvette, eliminating errors from cuvette
imperfections and scratches, eliminating the need to index the cuvette.

12
GENERAL OPERATIONS
6. GENERAL OPERATIONS
6.1. POWER CONNECTION & BATTERY MANAGEMENT
The meter can be powered from an AC / DC adapter (included) or from the built-in rechargeable
battery.
The meter will perform an auto-diagnostic test when it is first powered on. During this test, the Hanna
Instruments logo will appear on the LCD. After 5 seconds, if the test was successful, the last method
used will appear on the display.
The battery icon on the LCD will indicate the battery status:
• battery is charging from external adapter • battery fully charged (meter connected to AC / DC adapter)
• battery capacity (no external adapter) • battery near 0% (no external adapter)
• battery exhausted (no external adapter)
To conserve battery, the meter will turn off automatically after 15 minutes of inactivity (30 minutes
after a Zero measurement). If a photometer measurement is on the screen, an auto-log is created
before shutdown.
6.2. MODE SELECTION
The HI83303 has two operational modes: Photometer and Probe.
Photometer mode enables on-demand measurement of a cuvette using the integrated optical system.
Probe mode enables continuous measurement using a Hanna Instruments digital electrode connected
to the 3.5 mm port.
To switch between Photometer mode and Probe mode, use the MODE key.
Note: The active mode cannot be switched while in Setup, Recall or Method menus.

13
GENERAL OPERATIONS
6.3. GENERAL SETUP
Press the SETUP key to enter in Setup menu, highlight desired option using the  keys and
press Select.
CAL Check (Photometer Mode Only)
Press Select to enter the CAL Check screen. The date, time and values for the last CAL Check are
displayed on the screen. To start a new CAL Check press Check and follow the prompts on the screen.
See METER VALIDATION & CAL CHECK for additional information.
Temperature Unit (Probe Mode Only)
Option: °C or °F
Press the functional key to select the desired temperature unit.
Backlight
Values: 0 to 8
Press Modify to access the backlight intensity. Use the functional keys or the  keys to increase or
decrease the value. Press Accept to confirm or press the ESC key to return to the Setup menu without
saving the new value.
Contrast
Values: 0 to 20
Press Modify to change the display’s contrast. Use the functional keys or the  keys to increase
or decrease the value. Press Accept to confirm the value or the ESC key to return to the Setup menu
without saving the new value.

14
GENERAL OPERATIONS
Date & Time
Press Modify to change the date and time. Press the functional keys or the  keys to highlight the
value to be modified (year, month, day, hour, minute or second). Use the  keys to change the
value. Press Accept to confirm or ESC key to return to the Setup without saving the new date or time.
Time Format
Option: AM/PM or 24-hour
Press the functional key to select the desired time format.
Date Format
Option: DD/MM/YYYY, MM/DD/YYYY, YYYY/MM/DD, YYYY-MM-DD, Mon DD, YYYY,
DD-Mon-YYYY, YYYY-Mon-DD
Press Modify to change the date format. Use the  keys to select the desired format. Press Select
to confirm or the ESC key to return to the Setup menu without saving the new format.
Decimal Separator
Option: Comma ( , ) or Period ( . )
Press the functional key to select the desired decimal separator. The decimal separator is used on the
measurement screen and CSV (Comma-Separated Values) files.

15
GENERAL OPERATIONS
Language
Option: Português, Deutsch, English, Español, Français, Italiano, Dutch
Press Modify to change the language. Use the  keys to select the desired language. Press Select
to change the language.
Beeper
Option: Enable or Disable
When enabled, a short beep is heard every time a key is pressed. A long beep alert sounds when
the pressed key is not active or an error is detected. Press the functional key to enable or disable the
beeper.
Instrument ID
Option: 0 to 999999
This option is used to set the instrument’s ID (identification number). Press Modify to access the
instrument ID screen. Use the functional keys or the  keys to highlight the digit to be modified.
Press the  keys in order to set the desired value. Press Accept to confirm the value or press the
ESC key to return to the Setup menu without saving the new value.
Meter Information
Press Select to view the model, serial number, firmware version and selected language. Press the ESC
key to return to the Setup menu.

16
GENERAL OPERATIONS
Probe Information (Probe Mode Only)
Press Select to view model number, serial number and firmware version for the connected probe. Press
the ESC key to return to the Setup menu.
6.4. CONTEXTUAL HELP
HI83303 offers an interactive contextual help mode that assists the user at any time.
To access the help screen press the HELP key. The instrument will display additional information
related to the current screen. To read all the available information, scroll the text using the  keys.
Press the ESC key to return to the previous screen.

17
LOGGING DATA & DATA MANAGEMENT
7. LOGGING DATA & DATA MANAGEMENT
The instrument features a data log function to help the user to keep track of measurements. The data
log can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using
the LOG and RECALL keys.
7.1. LOGGING DATA
Press the LOG key and the last valid measurement will be stored with a date and time stamp. Only
valid measurements can be stored.
7.2. ADDING SAMPLE & USER NAMES TO LOG DATA
A sample ID and user ID can be added to the saved log. Use the  keys to highlight the Sample
ID or User ID then press Modify. Sample ID and user ID are entered using the alphanumeric multi-
tapping keypad.
Enter one character at a time by pressing the key with the assigned character repeatedly until the
desired character is highlighted. For reference, a list of the characters available for the current key will
be shown under the text box.
The character will be entered after a two-second delay or after another key is pressed.
Press Accept to update the sample or user ID.
Press functional key to delete the last character.
Press Clear to delete all of the characters.
Press the ESC key to discard all changes and return to the previous screen.

18
LOGGING DATA & DATA MANAGEMENT
7.3. DATA MANAGEMENT
Viewing & Deleting
Data can be viewed, deleted and exported to a USB drive or a PC by pressing the RECALL key. Use
the  keys to scroll through the saved logs. Press Info to view additional information about the
selected log.
Use Delete to erase logged data. After pressing Delete the prompt on the display will confirm the action.
Press No or the ESC key to return to the previous screen.
Press Yes to delete the selected log.
Press Del All to erase all the logged data. If Del All is pressed the prompt on the display will confirm
the action. Press Yes to delete all logged data, No or the ESC key to return to the log recall.
Data Export
Log data can be exported to a USB flash drive or to a PC. To access data export functions, press the
RECALL key then Export.
Use the  keys to select the desired export location.
For export to flash drive, insert the USB flash drive into the dedicated port at the back of the meter
labeled HOST USB, then follow the on-screen prompts.
For export to PC, connect the meter to a PC using the supplied micro-USB cable. Insert the cable into
the port at the back of the meter labeled PC PWR. Follow the on-screen prompts. When the display
informs that the PC is connected, the meter will appear as a removable disk. Use a file manager (such
as Windows Explorer or Mac Finder) to move the file from the meter to the PC.
Log data is exported as a single file (HI83303.csv) containing all logged photometer and probe data.
The CSV file may be opened with a text editor or spreadsheet application.

19
PHOTOMETER MODE
8. PHOTOMETER MODE
8.1. METHOD SELECTION
In order to select the desired method press the METHOD key and a screen with the available methods
will appear.
Press the  keys to highlight the desired method. Press Select.
After the desired method is selected, follow the procedure described in the related section.
Before performing a method, read all the instructions carefully.
8.2. COLLECTING & MEASURING SAMPLES AND REAGENTS
Proper Use of Syringe
1. Push the plunger completely into the syringe and insert the tip into the solution.
2. Pull the plunger up until the lower edge of the seal is exactly on the mark for the desired volume.
3. Take out the syringe and clean the outside of the syringe tip, be sure that no drops are hanging
on the tip of the syringe. Then, keeping the syringe in a vertical position, push the plunger down
into the syringe, the desired volume has been delivered.

20
PHOTOMETER MODE
Proper Use of Dropper Bottle
1. Tap the dropper on the table several times.
2. Remove the cap and wipe the outside of the tip with a cloth.
3. Keep the dropper bottle in a vertical position while dosing the reagent.
Proper Use of Powder Packet
1. Use scissors to open the powder packet.
2. Push the edges of the packet to form a spout.
3. Pour out the content of the packet.
8.3. CUVETTE PREPARATION
Proper mixing is very important for reproducibility of the measurements. The proper mixing technique
for each method is listed in the method procedure.
(a) Invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical
position. Turn the cuvette upside-down and wait for all of the solution to flow to the cap end,
then return the cuvette to the upright vertical position and wait for all of the solution to flow to
the cuvette bottom. This is one inversion. The correct speed for this mixing technique is 10 to 15
complete inversions in 30 seconds. This mixing technique is indicated with “invert to mix” and
one of the following icons: