Harvard Bioscience CMA 12 Elite User manual
Instructions for CMA 12 Elite Microdialysis Probe
TECHNICAL INFORMATION
Membrane
Material Polyarylethersulfone (PAES)
Molecular Cut-O 20,000 Daltons
Outer Diameter 0.5 mm
Length 1, 2, 3 and 4 mm
Probe Shaft
Material Stainless-steel or Metal Free
Diameter 0.64 mm
Length 14 mm
Internal Volume
Inlet Volume 0.2 µL
Outlet Volume 2.6 – 2.9 µL
CMA 12 Elite Microdialysis Probe User’s Manual
1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean,
at room temperature and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula
3. Connect the desired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet.
Tubing Adaptors and FEP Tubing should be used for all connections. To facilitate the handling of Tubing Adaptors,
they should be pre-soaked in ethanol for a minimum of 15 minutes.
4.
5.
Flush the probe with 10-15 μL/min in the Perfusion Fluid for 4-5 min to wash out air. While flushing, “tap” with a scissor
on the probe clip (not the probe) to remove air bubbles. The vibrations from the probe clip will in most cases remove the
air bubble. If possible, check for air bubbles under a stereomicroscope. If the air bubble is not gone, the flushing and
“tapping” must be repeated. When flushing the membrane it may appear to be “sweating” which is due to ultrafiltration
of fluid through the membrane.
6.
7. Set the pump to the required perfusion flow (usually 1-5 μL/min) and check for leaks. The microdialysis probe is now
ready for use.
8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL
INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling
times.
9. After the experiment, put the microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to
prevent salt crystal formation. The probe can be stored in deionized water.
Mount the microdialysis probe in the CMA 11 & 12 Probe Clip on the CMA/130 In-Vitro Stand or the In-Vitro holder
on the CMA 402 Pump. Put the microdialysis probe in a vial filled with perfusion fluid.
Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the
cannula. Wait for 10 min as the Tubing Adapter must be dry before flushing.
10.For further set up instructions, see CMA 120 System for Freely Moving Animals, User’s Manual.
WARRANTY
The probes manufactured by CMA Microdialysis are warranted to be free from defects in material and workmanship for a
period of two years from the manufacturing date if stored in the original package.
Claims should be forwarded without delay to CMA Microdialysis or to your local distributor.
ORDER INFORMATION Ref No.
CMA 12 Elite Microdialysis Probe, 1 mm, 3/pkg CMA 8010431
CMA 12 Elite Microdialysis Probe, 2 mm, 3/pkg CMA 8010432
CMA 12 Elite Microdialysis Probe, 3 mm, 3/pkg CMA 8010433
CMA 12 Elite Microdialysis Probe, 4 mm, 3/pkg CMA 8010434
CMA 12 Elite Metal Free, 1 mm, 3/pkg CMA 8011201
CMA 12 Elite Metal Free, 2 mm, 3/pkg CMA 8011202
CMA 12 Elite Metal Free, 3 mm, 3/pkg CMA 8011203
CMA 12 Elite Metal Free, 4 mm, 3/pkg CMA 8011204
CMA 12 Guide Cannula, 3/pkg CMA 8309024
CMA 12 Guide Cannula, 30/pkg CMA 8309025
CMA 12 Guide Metal Free, 3/pkg CMA 8011205
Tubing Adapter, 10/pkg CMA 3409500
FEP Tubing, 1 m, 1/pkg CMA 3409501
FEP Tubing, 1 m, 10/pkg CMA 8409501
Tubing Connector, 3/pkg CMA P000113
CMA 11 & 12 Probe Clip CMA 8309013
Perfusion Fluid CNS CMA P000151
Microsyringe 1 mL CMA 8309020
Microsyringe 2.5 mL CMA 8309021
Glass Ionomer Cement Kit CMA 750027
Trephine Drill Bits CMA 8011158
Anchor Screw Drill Bits CMA 8003264
Anchor Screws CMA 7431021
Anchor Screw Driver CMA 8309637
CMA 130 In-Vitro Stand CMA 8309102
CMA Microdialysis AB
Head Oce, Sweden
Torshamnsgatan 30A, SE-164 40 Kista, Sweden
Tel: +46 8 470 10 00
Harvard Apparatus
84 October Hill Road
Holliston, MA 01746 USA
Phone Orders: 800-232-2380 • Fax: 508-429-5732
8010437C
The CMA 12 Elite Microdialysis Probe is not intended for use in humans. It is only suitable for laboratory research in animals.
CMA Microdialysis only guarantees single usage of CMA 12 Elite Microdialysis Probes.
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