Hitachi L-2100 User manual

INSTRUCTION MANUAL
FOR
MODEL L-2100 PUMP
Copyright Hitachi High-Technologies Corporation 2002. All rights reserved. Printed in Japan. Part No. 890-9101

-1-
PREFACE
Thank you very much for purchasing Hitachi Model L-2100/2130
pump, which has been specifically designed for automatic
analysis in a liquid chromatograph system.
The Model L-2100/2130 pump is intended for use by persons
having a basic knowledge of chemical analysis.
Remember that improper use of analytical instruments,
chemicals or samples would result not only in wrong analytical
data but also in consequences adverse to safety.
Carefully read this instruction manual before attempting
operation. For proper use of the pump, please acquaint yourself
with it.
ABOUT THIS MANUAL
The operating procedures and maintenance/checkup instructions
for the L-2100/2130 pump are described in this manual.
First of all, read "IMPORTANT" and "SAFETY SUMMARY" at the
beginning of this manual for ensuring safety in operation of the
pump.
If installation of the pump is not yet completed, refer to Section 2.
Before attempting operation, read through Sections 3 and 4 to
become acquainted with the structure of the instrument and its
basic operations.

-2-

-3-
IMPORTANT
Precautions on Electromagnetic Wave Interference
1. Possible Electromagnetic Wave Interference Caused by This Instrument
Where this instrument is used in a residential area or an
adjacent area thereto, it may cause interference to radio and
television reception.
To prevent this, use the specified system connection cables in
strict accordance with the instruction manual. The instrument is
designed to minimize possible electromagnetic wave
interference caused by it if the specified cables are connected
properly.
However, there is no guarantee that electromagnetic wave
interference will not be caused by the instrument.
If the instrument does cause interference to radio or television
reception, which can be determined by turning the instrument off
and on, the user is encouraged to try to correct the interference
by one or more of the following measures:
•Reorient the antenna of the radio/television receiver.
•Increase separation between the instrument and the radio/TV
receiver.
•Connect the instrument to an outlet on a circuit different from
that to which the radio/TV receiver is connected.
2. Possible Electromagnetic Wave Interference Affecting This Instrument
Where this instrument is used near an intense electromagnetic
source, interference noise may be given to the instrument to
incur an adverse effect on its performance or functionality.
To present this, use the specified system connection cables in
strict accordance with the instruction manual. The instrument is
designed to minimize possible electromagnetic wave
interference affecting it if the specified cables are connected
properly.
However, there is no guarantee that electromagnetic wave
interference will not occur in the instrument. If the instrument
does incur electromagnetic wave interference, which can be
determined by turning on and off possible sources of
electromagnetic interference nearby, the user is encouraged to
try to correct the interference by one or more of the following
measures:

-4-
•Reorient the instrument.
•Increase separation between the instrument and possible
sources of electromagnetic interference.
•Connect the instrument to an outlet on a circuit different from
that to which possible sources of electromagnetic interference
are connected.
•Check that any other device connected with the instrument is
not affected by electromagnetic interference.
Warranty on Product
The Model L-2100 pump is warranted to be free from defects in
material or workmanship under normal use within the product
specifications indicated in this manual and under conditions
given below. This warranty is void if the instrument is not used
according to the instruction manual.
(1) Scope of Warranty
Any parts which prove to be defective in design or
workmanship during the warranty period will be repaired,
adjusted or replaced without charge. A substitute part may
be used for repair, or replacement with an equivalent
product may be made instead of repair. Such system
components as a personal computer and printer to be
updated frequently for improvement may not be available in
original versions at the time of replacement. Note that this
warranty does not apply to the instrument if modified by the
user or resold without permission from the manufacturer,
consumable parts, and any failure of lifetime-expired parts.
(2) Warranty Period
One year from the date of initial installation
(3) Availability of Technical Support Service
Technical support service for this instrument is available
within regular working hours on workdays specified by
Hitachi High-Technologies Corporation.

-5-
(4) Limitations and Exclusions on Warranty
Note that the following cases are excluded from the scope
of this warranty.
(a) Failure due to operation at a place not meeting the
installation requirements specified by Hitachi High-
Technologies Corporation.
(b) Failure due to power supply voltage/frequency other
than specified by Hitachi High-Technologies
Corporation or due to abnormality in power supply.
(c) Corrosion or deterioration of the tubing due to
impurities contained in reagent, gas, air or cooling
water supplied by the user.
(d) Corrosion of the electric circuits or deterioration of the
optical elements due to highly corrosive atmospheric
gas.
(e) Failure due to use of hardware, software or spare parts
other than specified by Hitachi High-Technologies
Corporation.
(f) Failure due to improper handling or maintenance by
the user.
(g) Failure due to maintenance or repair by a service
agent not approved or authorized by Hitachi High-
Technologies Corporation.
(h) After disposal of this instrument, or after its resale
without Hitachi High-Technologies Corporation
approval.
(i) Failure due to relocation or transport after initial
installation.
(j) Failure due to disassembly, modification or relocation
not approved by Hitachi High-Technologies
Corporation.
(k) Consumables, and failure of parts that have reached
the end of specified useful life.
(l) Failure of parts excluded from the warranty in the
instruction manual or other documents.
(m) Failure due to acts of God, including fire, earthquake,
storm, flood, lightning, social disturbance, riot, crime,
insurrection, war (declared or undeclared), radioactive
pollution, contamination with harmful substance, etc.
(n) Failure of the hardware, or damage to the system
software, application software, data or hard disk due to
computer virus infection.

-6-
(o) Failure of the personal computer connected with the
instrument, or damage to the system software,
application software, data or hard disk due to power
interruption or momentary power voltage drop caused
by lightning.
(p) Failure of the personal computer connected with the
instrument, or damage to the system software,
application software, data or hard disk due to
disconnection of main power to the personal computer
without taking the specified normal shutdown
procedure.
(5) Disclaimer of Warranty
THE MANUFACTURER MAKES NO WARRANTIES,
EITHER EXPRESS OR IMPLIED, EXCEPT AS PROVIDED
HEREIN, INCLUDING WITHOUT LIMITATION THEREOF,
WARRANTIES AS TO MARKETABILITY,
MERCHANTABILITY, FOR A PARTICULAR PURPOSE
OR USE, OR AGAINST INFRINGEMENT OF ANY
PATENT. IN NO EVENT SHALL THE MANUFACTURER
BE LIABLE FOR ANY DIRECT, INCIDENTAL OR
CONSEQUENTIAL DAMAGES OF ANY NATURE, OR
LOSSES OR EXPENSES RESULTING FROM ANY
DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.
NO ORAL OR WRITTEN INFORMATION OR ADVICE
GIVEN BY THE MANUFACTURER, ITS DEALERS,
DISTRIBUTORS, AGENTS OR EMPLOYEES SHALL
CREATE A WARRANTY OR IN ANY WAY INCREASE
THE SCOPE OF THIS WARRANTY.
(6) Warranty Card
The warranty card will not be reissued. Keep it in a safe
place.
Installation, Relocation and After-sale Technical Service
Installation of the instrument shall be carried out by or under
supervision of qualified service personnel of the manufacturer or
its authorized service agent.
Before installation of the instrument, the customer is requested
to prepare necessary utilities referring to this manual.
When relocation of the instrument becomes necessary after
initial installation (delivery), please notify your local Hitachi High-
Technologies sales representative or service office.

-7-
Technical Seminars and Training for Customers
For the customers to acquire in-depth understanding of the
analytical instruments, technical seminars and customer training
courses are available at Hitachi High-Technologies Corporation
or your site. For further information, contact your local Hitachi
High-Technologies Corporation sales representative.
(The technical seminars and customer training courses are
available on a chargeable basis.)
Other Precautions
1. Handling of Chemicals and Samples
(1) The user is responsible for following relevant legal
standards and regulations in the handling, storage and
discarding of chemicals and samples used in analytical
operations of the instrument.
(2) Reagents, standard solutions and accuracy-control
samples shall be handled, stored and discarded as
instructed by the respective suppliers.
2. Notice on This Instruction Manual
(1) The information contained in this manual is subject to
change without notice for product improvement.
(2) This manual is copyrighted by Hitachi High-Technologies
Corporation with all rights reserved.
(3) No part of this manual may be reproduced or transmitted in
any form or by any means without the express written
permission of Hitachi High-Technologies Corporation.

1 -
1
1. OUTLINE
The liquid chromatograph system consists of a pump for feeding
an eluent, a sample introducing section (autosampler, manual
injector) for injecting a sample, a column for chromatographic
separation of the injected sample, a column oven for maintaining
the column at a constant temperature, and a detector section for
detecting a signal of a separated component of the sample.
In addition, a degasser for deaerating an eluent, a gradient
device for varying a mixing ratio of eluents with time, and other
optional devices are incorporated in the system.
1.1 Isocratic System
The isocratic system has a simple configuration in which single-
solvent analysis is performed.
1.1
Model L-2100/2130 pump
Model L-2300 column oven
Model L-2200 autosampler
Model L-2400 UV detector
System power supply unit
Eluent
Drain
Washing solution
Degasser
Column

1 -
2
(1) An eluent is fed by the pump through the degasser.
(2) Asample is injected from the autosampler.
(3) The injected sample is separated through the column which
is maintained at a constant temperature by the column
oven.
(4) Each component separated from the sample is then
detected as a signal by the detector.
1.1 Isocratic System

1 -
3
1.2 Low-Pressure Gradient System
In the low-pressure gradient system, two or more solvents are
mixed in its low-pressure section from which a liquid feed is
performed by a single pump. The composition of an eluent is
made to vary with time in chromatographic separation.
(1) Each of plural eluents is fed from the degasser to the low-
pressure unit.
(2) In the low-pressure unit, the eluents are mixed at a pre-
specified ratio.
(3) Through the mixer, the mixture eluent is delivered.
(4) Asample is injected by the autosampler.
(5) The injected sample is separated through the column which
is maintained at a constant temperature by the column
oven.
(6) Each component separated from the sample is detected as
a signal by the detector.
1.2
Model L-2100/2130 pump
Model L-2300 column oven
Model L-2200 autosampler
Model L-2400 UV detector
Degasser
Washing solution
Mixer
Low-
pressure
gradient unit
Eluent
System power supply unit
Drain
Column

1 -
4
1.3 High-Pressure Gradient System
In the high-pressure gradient system, two or more solvents are
fed by respective pumps and mixed in the high-pressure section
for liquid delivery.
(1)From the degasser, each of plural eluents is applied to the
pump.
(2) The plural eluents are fed at a flow rate corresponding to a
pre-specified mixing ratio.
(3) The plural eluents thus fed are mixed in the high-pressure
gradient section. Through the mixer, the mixture eluent is
delivered.
(4) A sample is injected by the autosampler.
(5) The injected sample is separated through the column which
is maintained at a constant temperature by the column
oven.
(6) Each component separated from the sample is detected as
a signal by the detector.
1.3 High-Pressure Gradient System
Eluent
Drain
Model L-2100/
2130 pump
Model L-2200
autosampler
Model L-2300
column oven
Model L-2400
UV detector
System power
supply unit
Model L-2100/
2130 pump
Washing
solution
Mixer
Degasser
Column

2 -
1
2. INSTALLATION
CAUTION
Beware of heavy objects!
This instrument is as heavy as 15 kg.
When carrying this instrument, exercise care not to incur injury
by dropping it off accidentally. Be sure to hold the front and
rear parts of the instrument securely when moving it.
2.1 Unpacking
Carefully unpack the shipping case, take out module units and
standard accessories, and place them on a sturdy, level desk or
bench.
NOTICE: Installation of this instrument shall be carried out by
or under supervision of qualified service personnel
of Hitachi High-Technologies Corporation or its
authorized service agent.
Before installation of this instrument, the customer
shall make preparations for satisfying the
installation requirements in accordance with this
manual.
When relocation of this instrument becomes
necessary after initial installation (delivery), please
notify your local Hitachi High-Technologies sales
representative or service office.
2.1

2 -
2
2.2 Installation Place
When selecting an installation place for this instrument, be sure
to check the following conditions:
(1) Operating Ambient Temperature
4to 35 °C
Temperature variations during measurement should be
minimized. Take sufficient care to prevent dew
condensation.
(2) Operating Ambient Humidity
45 to 85% RH
(3) Atmosphere
(a) Well ventilated.
(b) Free from acidic or alkaline gases that could corrode
metals significantly.
(c) Free from vapors of solvents (e.g., benzene, thinner)
that could affect the paint coating on the instrument.
(4) Other Requirements
(a) Not exposed to direct sunlight.
Exposure to direct sunlight could result in deteriorated
performance or discoloration of the instrument.
Avoid installing the instrument near a window of the
laboratory room.
(b) Not exposed to drafts directly.
(c) Free from intense vibrations and shocks.
(d) Apart from a heat source such as a gas burner,
electric heater, or oven.
(e) Apart from a strong magnetism source such as an
electronic welder, high-frequency electronic furnace, or
voltage transformer.
2.2 Installation Place

2 -
3
(f) Free from an amount of dust and dirt.
Contamination with dust and dirt could deteriorate the
performance of the instrument.
(g) No abrupt fluctuations in power voltage.
Fluctuations in power voltage could cause noise in
analytical data.
(h) Do not connect the instrument to a power line used by
an electric device that is not equipped with a noise
suppressor circuit (e.g., stirrer, vibrator).
2.2

2 -
4
2.3 Items to be Prepared by the User
(1) Power voltage:100 to 240 V AC
Fluctuation.......Within ±10% with respect
to rated voltage
(2) Frequency :50 or 60 Hz
Fluctuation.......Within ±0.5 Hz with respect
to rated frequency
(3) Power
consumption :500 VA or more in total system
Provide power supply sufficient for
combinational use with other devices.
(4) Grounding :Grounding connection of Electric
Installation Technical Standard Class 3 or
higher should be provided.
WARNING
Ground Properly to Prevent Electric Shock Hazard!
•Be sure to use the power cable supplied with the
instrument. Use of a different power cable could result in
an electric shock hazard.
•This instrument is of a "plug-connected type" specified in
European Norm EN61010-1, so connect the power cable to
a grounded three-wire outlet.
•If a grounded three-wire outlet is not available, the power
cable may be connected to a two-wire outlet through an
adapter plug. In this case, be sure to provide proper
grounding connection as required.
2.3 Items to be Prepared by the User
2.3.1 Power Supply

2 -
5
For installation of this system, it is required to provide a space of
at least 640 mm (width) ×550 mm (depth). Prepare a sturdy,
level desk or bench capable of supporting a weight load of at
least 150 kg. On the rear of the main unit, allow at least
100 mm of clearance.
NOTICE:Installation of the instrument shall be carried out by
or under supervision of qualified service personnel
of the manufacturer or its authorized service agent.
Before installation of the instrument, the customer
is requested to prepare necessary utilities referring
to this manual.
When relocation of the instrument becomes
necessary after initial installation (delivery), please
notify your local Hitachi High-Technologies sales
representative or service office.
2.4 Checking the Contents
After unpacking, check the contents of each case against the
packing list attached to it.
2.3.2 Installation Space
2.3.2
Table tap with
ground cable
Power cord
Connector
L-2100/2130
Ground wire
(green)
Ground
terminal
Ground resistance:
Less than 100 ohms
Ground
Power outlet
More than
750 mm
under ground
Copper plate
300 mm
2 mm
10 mm

2 -
6
2.5 Assembling
The L-2000 Series system is provided with a system power
supply unit which is capable of connecting two pumps, one
autosampler, and one detector. The column oven has its own
power cord. In an instance where provision of a single system
power supply unit is insufficient for the use of two detectors, use
an AC adapter suitable for each instrument (50 W AC adapter
for the pump/autosampler, 100 W AC adapter for the detector).
The power cable from the system power supply unit and the AC
adapter power cable should be connected to the power
connector equipped on the left rear side of the instrument.
2.5.1Power Supply
Unit
2.5 Assembly
Rear of
instrument e-Line connector
Power connector

2 -
7
(1) Isocratic System/Low-Pressure Gradient System
Connect the power cables of the system power supply unit
and column oven to AC power source. For the L-2100/
2130 pump and L-2100 pump, connect the 50W cable
from the system power supply unit to the instrument power
connector. For the L-2400 UV detector, connect the 100
W cable from the system power supply unit to the
instrument power connector.
Model L-2100/2130
pump
Model L-2100
pump
Model L-2300
column oven
Model L-2400 UV
detector
To AC power
source
System power
supply nit 100 W cable
50 W cable
2.5.2

2 -
8
(2) High-Pressure Gradient System
Connect the power cables of the system power supply unit
and column oven to AC power source. For the L-2100/
2130 pump and L-2100 pump, connect the 50 W cable
from the system power supply unit to the instrument power
connector. For the L-2400 UV detector, connect the 100
W cable from the system power supply unit to the
instrument power connector.
In the L-2000 Series, connect the e-Line cable to the e-Line
connector equipped on the left rear of each instrument for
instrument-to-instrument wiring connection.
2.5.3Wiring
Arrangement
Model L-2100/
2130 pump
Model L-2100
pump
L-2300 column oven
Model L-2400
UV detector
To AC power
source
System power supply unit
100 W cable
50 W cable
Model L-2100/
2130 pump
Rear of
instrument e-Line connector
Power connector
2.5 Assembly