Hitachi CHROMASTER 5160 User manual

INSTRUCTION MANUAL
FOR
CHROMASTER 5160 PUMP
This product is intended for research use only. It is not to be used for reporting
patient diagnostic or therapeutic results.
Before using the instrument, read the safety instructions and
precautions carefully.
Be sure to observe the safety instructions in this manual and
the WARNING/CAUTION labels on the instrument.
Keep this manual in a safe place nearby so it can be referred
to whenever needed.
24-14, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo, Japan
3rd Edition, August 2014
Copyright C Hitachi High-Tech Science Corporation 2013. 1st Edition, 2012
All rights reserved. Printed in Japan. Part No. 892-9162-2

NOTICE:
1. The Information contained in this document is subject
to change without notice for improvement.
2. This manual is copyrighted by Hitachi High-Tech
Science Corporation with all rights reserved.
No part of this manual may be reproduced, transmitted
or disclosed to a third party in any form or by any
means without the express written permission of
Hitachi High-Tech Science Corporation.
3. Hitachi High-Tech Science Corporation assumes no
liability for any direct, indirect, or consequential
damages arising from use not described in this
manual.
Utmost care must be exercised when using the
instrument.
4. This document does not provide any warranty or
permission for industrial properties or any rights to
grant license lawfully and without infringement.

- 1 -
PREFACE
Thank you very much for purchasing Chromaster 5160 Pump.
The Chromaster 5160 Pump is specifically designed for automatic analysis in a liquid
chromatograph system.
Note that physiologically or biologically active samples are not applicable to the Chromaster
5160 Pump because of possible infection with bacteria or viruses.
This product is intended for use by persons having a basic knowledge of chemical
analysis.
Remember that improper use of analytical instruments, chemicals or samples would
result not only in wrong analytical data but also in consequences adverse to safety. Note
that it is allowed only for persons having a basic knowledge of chemical analysis
procedures to use this instrument.
Carefully read this instruction manual before attempting operation. For proper use of the
software, please acquaint yourself with it.
The liquid chromatograph system comprises a pump unit for delivering mobile phases, a
sample introduction unit for injecting samples (autosampler, manual injector), a column unit for
separating components of each sample, a column oven unit for maintaining the column at a
constant temperature, and a detector unit for detecting separated sample components as
electric signals. Before using the liquid chromatograph system, read carefully the instruction
manual accompanying each of these units so that you can carry out analysis properly.

- 2 -
ABOUT THIS MANUAL
This instruction manual describe how to use and maintain the Chromaster 5160 Pump.
This instruction manual. consists of the following sections.
IMPORTANT (Warranty, Installation, Relocation, After-sale Technical Service, etc)
SAFETY SUMMARY (collected and summarized)
Section 1 OUTLINE (Isocratic System, Low-Pressure Gradient System)
Section 2 FUNCTIONS (Name and Function of Each Part on Pump, Specifications)
Section 3 OPERATION (Basic Operation, UTLTY Setting, GLP and Preparation)
Section 4 MAINTENANCE & INSPECTION (Performance and Specifications Check)
Section 5 PARTS REPLACEMENT (Consumables and Spare Parts)
Section 6 INDEX
APPENDIX1INSTRATTION
APPENDIX2Description on Contact Signal Communication
APPENDIX3Terminology
First of all, read "IMPORTANT" and "SAFETY SUMMARY" at the beginning of this manual
for ensuring safety in operation of pump and accessories.

IMPORTANT - 1
IMPORTANT
Warranty on Product
Limited Warranty
The Chromaster 5160 Pump is warranted to be free from defects in material or workmanship
under normal use within the product specifications indicated in this manual and under
conditions given below. This warranty is void if the software is not used according to the
instruction manual.
The manufacturer makes no warranties, either express or implied, except as provided herein,
including without limitation thereof, warranties as to marketability, merchantability, for a
particular purpose or use, or against infringement of any patent.
No oral or written information or advice given by the manufacturer, its dealers, distributors,
agents or employees shall create a warranty or in any way increase the scope of this warranty.
Scope of Warranty
Any parts which prove to be defective in design or workmanship during the warranty period will
be repaired, adjusted or replaced without charge. A substitute part may be used for repair, or
replacement with an equivalent product may be made instead of repair. Such system
components as a personal computer and printer to be updated frequently for improvement may
not be available in original versions at the time of replacement.
Note that this warranty does not apply to the instrument after it is discarded, or if modified by
the user or resold without permission from the manufacturer, consumable parts, and any failure
of lifetime-expired parts.
The manufacturer assumes no liability for any damage to data or application software due to
any possible fault or failure of this instrument.
Warranty Period
One year from the date of initial installation.
(In case a separate warranty document has been issued, the warranty period indicated in it
takes precedence over the above period.)

IMPORTANT - 2
Warranty Limitations and Exclusions
Disclaimer of Warranty
THE MANUFACTURER MAKES NO WARRANTIES, EITHER EXPRESS OR IMPLIED,
EXCEPT AS PROVIDED HEREIN, INCLUDING WITHOUT LIMITATION THEREOF,
WARRANTIES AS TO MARKETABILITY, MERCHANTABILITY, FOR A PARTICULAR
PURPOSE OR USE, OR AGAINST INFRINGEMENT OF ANY PATENT. IN NO EVENT SHALL
THE MANUFACTURER BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY
DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.
NO ORAL OR WRITTEN INFORMATION OR ADVICE GIVEN BY THE MANUFACTURER, ITS
DEALERS, DISTRIBUTORS, AGENTS OR EMPLOYEES SHALL CREATE AWARRANTY OR
IN ANY WAY INCREASE THE SCOPE OF THIS WARRANTY.
Note that the following cases are excluded from the scope of this warranty, i.e., these cases are
beyond the coverage of free-of-charge repair even during the warranty period indicated above.
(a) Failure due to operation at a place not meeting the installation requirements specified by
the manufacturer.
(b) Failure due to power supply voltage/frequency other than specified by the manufacturer or
due to abnormality in power supply.
(c) Corrosion or deterioration of the tubing due to impurities contained in reagent, gas, air or
cooling water supplied by the user.
(d) Corrosion of the electric circuits or deterioration of the optical elements due to highly
corrosive atmospheric gas.
(e) Failure due to use of software, hardware or spare parts not supplied by the manufacturer.
(f) Failure due to use not described in the manual or improper repair not approved by the
manufacturer.
(g) Failure due to maintenance or repair by other than service personnel qualified by the
manufacturer.
(h) Failure due to relocation or transport conducted not under the supervision of the
manufacturer after the initial installation of the instrument.
(i) Failure due to disassembly, modification or relocation not approved by the manufacturer.
(j) Failure due to acts of God, including fire, earthquake, storm, flood, lightning, social
disturbance, riot, crime, insurrection, terrorism, war (declared or undeclared), radioactive
pollution, contamination with harmful substance, etc.

IMPORTANT - 3
(k) Failure of the hardware, or damage to the system software, application software, data or
hard disk due to computer virus infection.
(l) After disposal of this instrument, after its resale without prior approval from the
manufacturer, consumable parts, and failure of any part that have reached the end of its
service life.
(m)Failure due to a life-limited part that has exceeded the end of its useful lifetime.
Limitations of Liability
Hitachi High-Tech Science shall not be liable in contract or in tort (including, without limitation,
negligence, strict liability or otherwise) for claims by third parties (other then for bodily injury or
property damage), for economic losses of any kind or for any special, incidental, indirect,
consequential, punitive or exemplary damages, arising in any way out of the performance of, or
failure to perform, this instrument, even if Hitachi High-Tech Science could foresee or has been
advised of the possibility of such damages.
Disclaimer of Liability for Industrial Properties of a Third Party
Hitachi High-Tech Science does not assume any liability for a third party’s complaint regarding
infringement of any patent rights or industrial properties with respect to products manufactured
through use of the equipment supplied by Hitachi High-Tech Science or its related companies
or application of said instrument.
Installation, Relocation and After-sale Technical Service
Installation and Relocation
(a) Installation at delivery shall not be carried out by the user. It shall be carried out by our
sales representative or the engineers who have been trained and qualified for this
purpose by us in order to use the instrument safely and accurately.
(b) Before installation, the user shall make preparations for satisfying the installation
requirements in accordance with this instruction manual.
(c) If relocation becomes necessary after initial installation (delivery), please contact the
dealer from whom you purchased the instrument or our sales representative.
After-sales Service
(a) For after-sale technical service of this instrument, please notify our local sales
representative or service office.

IMPORTANT - 4
(b) A maintenance & service contract is available for servicing the instrument after the
warranty period has ended (service available at charge).
(c) Maintenance parts and consumables will be supplied during the useful life of this
instrument (7 years). And even though parts and/or units (when available) will continue
to be supplied after the specified useful life has ended (for up to 10 years), they are not
intended to extend the useful life of the instrument. Also, substitutes may be supplied in
the event of discontinuance of manufacture of certain parts or units.
(d) Some main components of the instrument other than maintenance parts and
consumables may not be available in the event of discontinuance of manufacture of the
main unit. If repair cannot be provided in the case of instrument malfunction, we
recommend that you consider discontinuing use and replacing the instrument.
Technical Seminars and Training Courses for Users
We offer technical seminars and training courses at either our or user’s facilities to ensure
proper and safe operation of the analytical instrument to its full performance. For further
information, contact our sales representative. (Applicants will be charged.)
Estimated Life time of the Instrument
This instrument has a useful service life of seven (7) years after the date of initial use
(installation), which is estimated under the condition that periodic maintenance, checkup,
replacement of life-limited parts, and repair of worn parts are carried out as specified in the
instruction manual.

IMPORTANT - 5
Caution on Disposal of Instrument
Although at present the instrument does not use materials that would directly cause
environmental disruption, the environmental protection laws and regulations may be revised or
amended. When planning to dispose of the instrument, therefore, be sure to check the latest
issues of the relevant laws and regulations or consult our sales representative.
Disposal of This Instrument and Its Parts
In the present design, this instrument does not use materials that would directly cause
environmental harm. Note, however, that the environmental protection laws and regulations
may be revised or amended. Therefore, be sure to consult with your local Hitachi
High-Technologies sales or service representative when planning disposal of this instrument or
its parts and accessories.
When discarding or recycling the used PC, it is the user’s responsibility to delete all data from
the hard drive.
When discarding the chargeable battery mounted on the PC, refer waste disposal treatment
servicing to an authorized waste collection agent, or send the battery for recycling after
providing insulation on its positive and negative terminals with adhesive vinyl tape or the like.
Precautions on CE Conformity Marking
In consideration of use in the European countries, this instrument bears the CE mark indicating
the conformity to the requirements mentioned below.
Electromagnetic Compatibility Requirement
This instrument is designed to satisfy the European Norm EN61326-1 (2006) for the CE
conformity marking through conformity to the EMC Directive 2004/108/EC.
This instrument is classified as Class A of EN61326-1. So, this instrument must not be used in
domestic establishments nor in establishments directly connected to a low voltage power
supply network which supplies buildings used for domestic purpose.
And this instrument is also designed to comply with table 1 "Basic immunity test requirements"
in the above European Norms. If the instrument is used near an intense electromagnetic
source, however, interfering noise may be given to the instrument to cause an adverse effect
on its performance or functionality.
Safety Requirement
This instrument is also designed to satisfy the European Norm EN61010-1 (2010) for the CE
conformity marking through conformity to the LVD Directive 2006/95/EC.
This instrument is requested to be used in a suitable environment and grounded appropriately.

IMPORTANT - 6
Information for Users on WEEE (only for EU Countries)
This symbol is in compliance with the Waste Electrical and
Electronic Equipment directive 2002/96/EC (WEEE).
This symbol on the product indicates the requirement NOT to
dispose of the equipment as unsorted municipal waste, but use the
return and collection systems available.
Information on Disposal for Users
1. In the European Union
If you need to discard this product or discard user serviceable parts:
Please contact your local sales representative or distributor who will inform you of the recycle of
the product. You might be charged for the costs arising from take-back and recycling.
2. In other Countries outside the EU
If you wish to discard this product, please contact your local authorities and ask for the correct
method of disposal.
Other Precautions
Handling of Chemicals and Samples
(a) The user is responsible for following relevant laws and regulations in handling, storage and
disposal of chemicals and samples used in analytical operation with this instrument.
(b) Reagents, standard solutions and accuracy-control samples shall be handled, stored and
discarded as instructed by the respective suppliers.
(c) Physiologically or biologically active samples are not applicable to this instrument because
of possible infection with bacteria or viruses.

IMPORTANT - 7
Disturbance by Electromagnetic Wave
This instrument conforms to Class A in the EN standards EN61326 (first edition 2002-02).
Avoid installing this instrument near equipment whose data will be affected by
electromagnetic noise within the permissible range of this standard.
Also, data of this instrument may be affected by electromagnetic noise or the instrument itself
may malfunction. In the room where this instrument is installed, the following electric
devices must not be brought: Devices which emit radio waves such as mobile phone,
transceiver, wireless telephone and similar small-power device.

SAFETY - 1
SAFETY SUMMARY
Before using the Chromaster 5160 Pump, be sure to read the following safety
instructions carefully.
The hazard warnings which appear on the warning labels on the product or in the manual have
one of the following alert headings consisting of a safety alert symbol and signal word
WARNING or CAUTION.
:
The alert symbol shown at left precedes every signal word for hazard
warnings, and appears in safety-related descriptions in the manual.
To prevent possible hazards or injury, be sure to follow the safety
precautions preceded by this symbol.
WARNING : Indicates a potentially hazardous situation which, if not avoided, will or
can result in death or serious injury.
CAUTION : Indicates a potentially hazardous situation which, if not avoided, will or
can result in minor or moderate injury.
The following signal word NOTICE or NOTE is used to indicate precautionary instructions
concerning possible property damage.
NOTICE ：Used to indicate a potentially hazardous situation which, if not avoided,
will or can result in damage to user’s property, serious damage to the
instrument, damage to data, or environmental pollution, though
personal injury may not be incurred.
NOTE ：Used to indicate explanatory information for ensuring proper instrument
operation and accurate measurement while preventing any possible
damage to the instrument.
Be sure to observe the precautionary instructions in the manuals accompanying the
instrument.
Do not perform any operation or action other than described in these manuals.
On occurrence of any trouble in the instrument, notify the nearest sales service
representative of Hitachi High-Technologies.
Keep in mind that the hazard warnings in the manuals or on the product cannot cover every
possible case, as it is impossible to predict and evaluate all circumstances beforehand.
Always be alert and use your common sense.

SAFETY - 2
SAFETY SUMMARY
General Safety Precautions
Safety Precautions During Use
WARNING
If an abnormal condition such as unusual noise, odor or fumes occurs during
operation of the instrument, the power supply should be turned off immediately.
Disconnect the power cord plug from the power outlet. Then, after providing proper
safety measures as required, contact the nearest service representative of Hitachi
High-Technologies.
Using the instrument in such an abnormal condition could result in an electric shock or
fire.
Radiation from the Laser Light Source
WARNING
The internal CD-ROM or CD-R/W drive of the PC is provided with a laser beam
source.
In normal operation of the PC containing the laser beam source, no laser beam
harmful to human health will be emitted outside. Note, however, that if a laser beam
leaking out of the PC accidentally gets into the eye, eye injury could occur. When
using the PC, carefully read the laser-related safety precautions described in the PC
manual. Be sure to observe these instructions and the following precautions:
(a) Do not open the panel of the laser device. There are no user-serviceable parts
inside.
(b) On any other laser device, do not attempt to operate or adjust.

SAFETY - 3
SAFETY SUMMARY
WARNING Precautions in the Manual
WARNING
Ignition of Flammable Chemicals
This instrument is not explosion-proof. In unattended operation, do not use organic
solvents having an ignition point below 70 C (Section 3.4, 3.6)
Beware of ignition hazard when using flammable chemicals such as organic solvents.
(a) Do not bring a heat or flame source near the instrument.
(b) Well-ventilate the laboratory room where the instrument is used.
(c) Always check the following conditions. If an abnormality is found, stop operation
immediately.
Leakage of solvent or waste solution.
Leakage of solvent inside the instrument. (Section 3.4, 3.6)
When using flammable chemicals, be careful about possible ignition due to static
electricity. To prevent the build-up of static electricity, use a conductive container for
waste solution employ and provide proper grounding connection to it.
(Section 3.4, 3.6)
Explosion of Vapor from Flammable Chemicals
If a flammable chemical such as organic solvent leaks from the flow path of the instrument
and its vapor concentration reaches the explosion limit, it could cause spontaneous
combustion with dangerously explosive results.
When using a flammable and readily volatile chemical, be sure to check for leakage from
the instrument flow path and ventilate the laboratory room adequately. (Section 3.4, 3.6)

SAFETY - 4
SAFETY SUMMARY
WARNING Precautions in the Manual (Continued)
WARNING
Electric Shock due to Contact with Inside of Instrument
When removing the pump cover for part replacement, etc., there is a risk of electric shock.
Be sure to turn OFF the power switch and disconnect the power cord from the receptacle
in advance. (Section 4.3)
Electric Shock due to Improper Grounding
This instrument is designed in conformity with the specifications of Class I in Annex H of
the IEC 61010-1 (International Electrotechnical Commission Standards) – Issue 1.
To prevent an electric shock hazard, provide a proper grounding connection.
(a) Be sure to use the grounded 3P power cable, which is supplied as a standard
accessory for the instrument.
The use of a different type of power cable may result in an electric shock hazard.
Connect the 3P power cable to a grounded 3P power outlet.
(b) If a grounded 3P power outlet is not available, use a grounded 3P table tap or a
3P-2P adapter. In this case, be sure to provide proper grounding connection.
For grounding connection, use a screw having a diameter of M4 or higher and a
turn count of 3 or more in threading, and a wire having a thickness of 1.25 mm.
(APPENDIX 1)
Inflammation or Injury due to Toxic, Corrosive or Stimulative Solvent
When using a toxic, corrosive or stimulative solvent, be careful not to incur a physical
inflammation or injury. For details of the properties of each solvent and how to handle it,
refer to the relevant Material Safety Data Sheets (MSDS). Be sure to handle each
solvent properly.
(a) Wear proper protective clothes (e.g., safety goggles) so that a solvent will not
come into direct with the skin.
(b) (b) Ventilate the laboratory room adequately to prevent accidental inhalation of
harmful solvent vapor. (Section 3.4, 3.6)

SAFETY - 5
SAFETY SUMMARY
CAUTION Precautions in the Manual
CAUTION
Heavy Instrument
This instrument is as much as 16 kg. When carrying this instrument, exercise care not to
incur injury by dropping it off accidentally. (APPENDIX 1)
Prevention of Instrument Tipover
Personal injury could be incurred if the instrument tips over. When installing the
instrument, provide a proper means for preventing the instrument from tipping over
accidentally. (APPENDIX 1)
Fatigue due to Long-Hour Operation
If you keep working with the display monitor and keyboard for long hours, your eyes and
body will be fatigued to jeopardize your health. When working with the display monitor
for a long time, take a break for 10 to 15 minutes per hour for health of your eyes and
body. (Chapter 3)
Injury of Hand by Seal remover PV
When removing a seal from the Manual Purge Valve, the seal remover of PV can injure
your hand or finger. Carefully read this instruction manual before starting operation, and
use care with Seal remover PV. (Section 5.4)

SAFETY - 6
SAFETY SUMMARY
WARNING and CAUTION Labels on Instrument
The warning labels shown below are attached on the Chromaster 5160 Pump.
Read the warning labels carefully, and check the instructions on them to attain a clear
understanding with reference to actual parts. Periodically check the appearances of these
warning labels to see if they are clean to allow easy reading over a safe distance. If any
one of the warning labels becomes illegible due to deterioration, contact your local Hitachi
High-Technologies Corporation sales representative or service office of Hitachi
High-Technologies Corporation sales representative for replacement with a new one.
(1) Ignition of Flammable Chemicals (2) Explosion of Vapor from Flammable Chemicals
Right Side View
Inside of left door

SAFETY - 7
SAFETY SUMMARY
NOTICE Precautions
Precautions on Use of Solvents
Fluororesin, PEEK, quartz, Vespel® and SUS316 materials are used for the wetted parts
of the instrument. Never use solvents that would corrode these materials.
This instrument has internal parts made of materials that could be corroded by strongly
acidic solutions, strongly alkaline solutions or organic solvents. Take care not to spill
these corrosive solutions or solvents into the inside of the instrument. If a corrosive
solution or solvent spills inside, wipe it off immediately.
For use of the liquid leak sensor, follow the instructions given below.
(a) Turn on the leak sensor function when feeding a solvent.
(b) The leak sensor function may remain inactive in cases where the amount of leakage
is too small. Furthermore, leakage of a highly volatile solvent may not be detected.
Be sure to check for liquid leakage regularly to prevent a possible serious accident
regardless of whether the leak sensor is activated or not.
(a) In routine inspection of the instrument, visually check for liquid leakage before
attempting measurements.
(b) In periodic inspection of the instrument, check that the leak sensor works normally.
(Section 2.5)
Referring to the instruction manual accompanying each module unit of the liquid
chromatograph system, set up the automatic liquid feed stop function (such as the
pressure limiter function of the pump unit) to prevent a possible trouble. (Section 4.3)
Disposal of Waste Solution
Be sure to collect waste solution and treat it for proper disposal in accordance with the
relevant laws and regulations regarding water pollution control and sewage treatment.
Improper treatment of waste solution may result in environmental pollution and could also
lead to a penalty.

SAFETY - 8
SAFETY SUMMARY
NOTICE Precaution (Continued)
Accuracy and Precision of Measured Values
Carry out periodic inspection and check whether the system is operating normally.
If necessary, conduct measurement on a control sample.

- i -
CONTENTS
PREFACE...............................................................................................................1
ABOUT THIS MANUAL..........................................................................................2
IMPORTANT.....................................................................................IMPORTANT-1
Warranty on Product ................................................................IMPORTANT-1
Installation, Relocation and After-sale Technical Service ....IMPORTANT-3
Technical Seminars and Training Courses for Users ...........IMPORTANT-4
Estimated Life time of the Instrument.....................................IMPORTANT-4
Caution on Disposal of Instrument.........................................IMPORTANT-5
Disposal of This Instrument and Its Parts..............................IMPORTANT-5
Precautions on CE Conformity Marking.................................IMPORTANT-5
Information for Users on WEEE (only for EU Countries)......IMPORTANT-6
Other Precautions.....................................................................IMPORTANT-6
SAFETY SUMMARY ........................................................................ SAFETY-1
General Safety Precautions........................................................... SAFETY-2
WARNING Precautions in the Manual........................................... SAFETY-3
CAUTION Precautions in the Manual............................................ SAFETY-5
WARNING and CAUTION Labels on Instrument........................... SAFETY-6
NOTICE Precautions....................................................................... SAFETY-7
1. OUTLINE................................................................................................................1-1
1.1 Isocratic System..........................................................................................1-1
1.2 Low-pressure Gradient System..................................................................1-2
2. FUNCTIONS...........................................................................................................2-1
2.1 Name and Function of Each Part on Pump................................................2-1
2.2 Name and Function of Each Part on UI Pad (Option)................................2-6
2.3 Specifications ..............................................................................................2-10
3. OPERATION ..........................................................................................................3-1
3.1 Basic Operation...........................................................................................3-1
3.1.1 Power Supply...................................................................................3-1
3.1.2 Parameter Setting............................................................................3-7
3.1.3 Operational State.............................................................................3-9
3.1.4 ON/OFF Setting...............................................................................3-10
3.1.5 Purging Operation............................................................................3-10
3.1.6 Manual Operation ............................................................................3-11
3.1.7 Programming Operation...................................................................3-13
3.1.8 Exit Operation..................................................................................3-15
3.1.9 Washing after Operation..................................................................3-15