HMC HG-50 User manual

(Form: CE-014a-01)
HG-50/HG-80
OPERATING MANUAL
● Be sure to read this operation manual carefully and handle it properly.
WARNING
!

－1－
Introduction
(1) No part of this document may be reproduced without permission.
(2) The contents of this document are subject to change without notice.
(3) This document has been carefully compiled. If you have any questions or require
information not covered in the manual, please contact :


Hafing 21
84549 Engelsberg
Tel.: [phone]
Fax.:[phone]
[e-mail]

－2－
Read Carefully Before Using
●Determine the handling person responsible of this product.
●In this manual the following headings are applied to items to which great attention should be
given:
WARNING : Precaution indicating an imminent dangerous situation which if not
avoided may lead to death or serious injury.
CAUTION : Precaution indicating a dangerous situation which if not avoided may
lead to moderate or slight injury.
IMPORTANT : Indicates items you are strongly advised to obey.
󰁅
󰁅󰁅
󰁅 NOTE : Items that will aid in proper operation of the equipment.
●Never use the autoclave to sterilize any of the following hazardous materials or substances with
alkali content. Sterilization of such objects can cause explosion, corrosion of the working
chamber or chamber piping, and deterioration of gaskets.
List of Hazardous Materials
Explosive substances
・Nitroglycol, nitroglycerin, nitrocellulose, and other explosive nitric esters.
・Trinitrobenzene, trinitrotoluene, picric acid, and other explosive nitro compounds.
・Peracetic acid, methyl ethyl ketone peroxide, benzoyl peroxide, and other organic peroxides.
Ignitable substances
・Metallic lithium, potassium, sodium, yellow phosphorous, phosphorus sulfide, and red
phosphorus.
・Celluloids, calcium carbide (carbide), lime phosphide, and magnesium powder
・Aluminum powder, magnesium powder, and metallic powders other than aluminum powder
・Sodium dithionite (or sodium hydrosulfite)
Oxidizing agents
・ Potassium chlorate, sodium chlorate, ammonium chlorate, and other chlorates
・ ・ Potassium perchlorate, sodium perchlorate, ammonium perchlorate, and other
perchlorates.
・Potassium peroxide, sodium peroxide, barium peroxide, and other inorganic peroxides
・Potassium nitrate, sodium nitrate, ammonium nitrate, and other nitrates
・Sodium chlorite and other chlorites
・Calcium hypochlorite and other hypochlorites
Flammable substances
・Ethyl ether, gasoline, acetaldehyde, propylene oxide, carbon disulfide, and other substances
whose flash points range from -30 to 0°C.
・Methanol, ethanol, xylene, benzyl acetate (or amyl acetate), and other substances whose
flash points range from 0 to 30°C.
・Kerosene, gas oil, turpenine oil, isopentyl alcohol (or isoamyl alcohol), acetic acid, and other
substances whose flash points range from 30 to 65°C.
Flammable gas (hydrogen, acetylene, ethylene, methane, ethane, propane, butane, and other
substances that are gases at a temperature of 15°C under 1 atmospheric pressure.)
WARNING
!
!
!
!

－3－
● When liquid with salt water and much salinity of salt agar etc.
spills in the chamber, blowing, discharge water in the
chamber and wipe up drop of water around the lid gasket
beautifully.
It causes the corrosion of the chamber and the piping when
leaving just as it is
● Check that the pressure is below "0MPa" before opening the
lid.
● Absolutely do not attempt to remodel or alter this product.
●Foreign matter (metals, liquid) may enter through the vent hole. Operating the equipment with
such foreign matter inside may cause trouble with the equipment, fire or electric shock.
●Do not forcibly bend, twist, tie or extend the power cord. Do not place heavy objects on the cord.
A damaged cord or exposed wire can cause fire or electric shock.
●Never connect the power cord to a power supply other than one of the rated voltage. Connection
to such a power supply can cause fire or electric shock.
●If grounded socket is unavailable, ground the equipment using a separate ground wire before
connecting the power cord to the power source.
●Connect the grounding cable correctly to the Type D or higher-grade grounding terminal. Never
connect the grounding wire to gas pipes or water pipes.
●Close the lid after confirming that no foreign matter is adhering to the section contacting the lid
gasket. Foreign matter in this section can cause vapor leaks.
●When using a waste processing bag or other kind of bag and disinfecting, place the bag in the
metal mesh holder and then insert it into the chamber. Using the bag “as is” can cause excessive
temperatures, pressures, lack-of-water, etc.
●Be careful not to pinch your hands when closing the lid.
●Do not touch the lid or lid cover when opening or closing the lid.
●Do not put your face or hands close to the chamber when lifting the lid after operations are
complete; steam will gush out of the chamber.
●The lid, chamber, gasket and panel are extremely hot immediately after the completion of
operation. Do not touch the equipment or you may get burned.
●Put on heat insulating gloves before removing a substance from the chamber. Do not put hands
into the chamber until the steam has been vented.
●Some time is required for liquids to cool. Be sure to check that the temperature has dropped
sufficiently before unloading a liquid from the chamber or burns can result.
●Do not unload the exhaust bottle or drain the chamber when the chamber is under pressure.
Boiling water or steam may gush out causing burns.
●Do not remove the exhaust bottle before water in the bottle has sufficiently cooled.
●If any abnormality occurs (e.g. abnormal sounds, smells, smoke), immediately shut the power off.
After checking to see that the abnormal condition does not continue, call our authorized
distributor in your region.
●If the display reading changes between the steps, turn the POWER switch off then on again. If
the problem continues, turn the power switch off and call our authorized distributor in your region.
CAUTION
!
0.2
0.3
0.4
0
0.1
0
Pressure
gauge

－4－
How to Read this Manual
●This operation manual consists of the following sections covering the information required for
proper operation of the Autoclave HG-50.
Chapter 1. What is the Autoclave HG-50? :
This section describes the uses and features of the product and the names and functions of its
parts.
Chapter 2. Installation :
This section explains where the equipment should be installed and how to install it. The product
incorporates precision parts, so be sure to follow the instructions covered in this chapter.
Chapter 3. Operation Method :
This section illustrates how to change various set values, and describes operations before
starting the equipment and after automatic operation. This section also covers the display and
performance of the equipment during automatic operation.
Chapter 4. Maintenance and Service :
This section explains the methods for draining water from the exhaust bottle or chamber,
cleaning the body of the equipment, and parts replacement.
Chapter 5. Specifications
This section includes dimensions, power consumption and working range of the product.
Refer to this section as is required.
Chapter 6. Troubleshooting
This section covers troubleshooting procedures for the product. If you encounter a problem, read
this section first.
Appendix
This section contains information on the warranty and a glossary of terms that appear in the
manual. Please refer to this section when necessary.

－5－
CONTENTS
Introduction·················································································································· 
Read Carefully Before User Inspection ···································································· 
How to Read this Manual ·························································································· 
CONTENTS ·················································································································· 
Chapter 1. What is the Autoclave HG-50?·············································1
1. Product Uses··········································································································· 1
2. Product Features····································································································· 1
3. Names and Functions of Each Part ········································································· 1
Chapter 2. Installation·············································································3
1. Installation Instructions ···························································································· 3
2. Installation Procedure······························································································ 4
Chapter 3. Operation Method ·································································6
Basic Operation Method ·························································································· 6
1. Power ······················································································································ 7
2. Pouring Water·········································································································· 7
3. Loading Substance·································································································· 8
4. Selection of Course and Program············································································ 9
5. Changing Set Values (Registering of Values by Customer) ··································· 11
6. Changing Function Setting ···················································································· 14
7. Reservation Operation··························································································· 16
8. Starting Operation·································································································· 18
9. Unloading ·············································································································· 19
10. After Completion of Operation ············································································· 19
11. To Interrupt Operation ·························································································· 20
12. If Power Supply is Cut Off during Operation ························································ 20
13. Operation of Cycles ····························································································· 20
Chapter 4. Maintenance and Service···················································24
1. Draining Exhaust Bottle ························································································· 24
2. Draining Chamber ································································································· 25
3. Cleaning Chamber································································································· 25
4. Cleaning Body ······································································································· 26
5. Water Supply to Cooling Tank················································································ 26
Chapter 5. Specifications ·····································································27
Chapter 6. Troubleshooting ·································································28
1. Error Detection (Alarms) ······················································································ 28
2. Early Troubleshooting···························································································· 29
Appendix································································································30
1. Fast wearing parts ································································································· 30
2. Glossary ················································································································ 30
3. Limited Warranty ··································································································· 31

－6－
Chapter 1. What is the Autoclave HG-50?
1. Product Uses
・The product is used to sterilize substances which can withstand high temperature and high
pressure steam such as tools of glass, ceramic, metal or rubber, water, media, reagents and
liquid medicines (Mode 1 – 3) .
・The product is also used to liquefy media (Mode 4).
2. Product Features
・In order to secure safety, the product is provided with a lid cover around the section that
becomes hot during operation,.
・The lid of the product is opened and closed automatically. It is not necessary to bring your
hands near the chamber port to open or close the lid. The safety has been improved this way.
・The agar cycle, which prevents solidification of sterilized media when they are not taken out
immediately, and the dissolution cycle, which dissolves solidified media, are provided.
・When the exhaust valve (the opening of the exhaust valve) is set, fine exhaust is conducted
automatically after sterilization.
・Steam that comes out of the exhaust valve during air release and exhaust is cooled in the
cooling tank to minimize the quantity of steam that is emitted into the room.
・The temperature, at which the loaded substance cannot be taken out, can be set for safety within
the range of 60-97℃for each cycle and program number.
・A reservation timer is provided to permit operation startup at any desired time within the range of
1 hour to 99 hours later.
3. Names and Functions of Each Part
●
●●
●Outer View of Body
F. L
Lid cover
Operation panel
Pressure gauge
Drain bottle
Drain valve
Caster
Vent hole
Power cord
Main power switch
Cooling fan
Vent hole
Cooling tank
Water filler port
Lid
Gasket
Chamber
0.2
0.3
0.40
0.1
Top panel
Top View
Front View Right Side View Rear View
When the lid is open

－7－
●
●●
●Operation panel
Digital Display (Temperature)
The digital display indicates the sterilization set time in the standby state, and it indicates the
chamber temperature during operation.
Digital Display (Time and Pressure)
The digital display indicates the set time, reservation time, etc. in the standby state, and it indicates
the pressure inside the chamber, time remaining until completion of sterilization, and time
remaining until completion of warming during operation.
Digital Display (Warming)
The digital display indicates the warming set temperature, function number, etc. in the standby
state, and it indicates the error when it is detected.
Unit Display ( , , , and )
The unit corresponding to the current digital display illuminates.
State Display ( , , and )
The state display indicates the present state.
Cycle Display
The action cycle of the preset cycle illuminates, and the current cycle blinks.
Drain Bottle Display
The red light blinks when the drain bottle is full, and the green light illuminates when it is not full.
POWER Switch
Used to turn on or off the power to the autoclave.
LID CLOSE Switch
Used to close the lid.
LID OPEN switch
Used to open the lid. When the lid can be opened, a green light comes on, and when the lid
cannot be opened because the chamber is hot and highly pressurized, a red light comes on.
Cycle Switch
Used to select the cycle and program number.
FUNC. Switch
Used to change and confirm the setting of respective functions.
SET switch
Used to change and confirm the set value.
Set Value Increase/Decrease Switch (󰁄󰁎)
Used to increase or decrease the set value.
Exhaust Level Switch ()
Used to change the exhaust level.
FAN Cooling ON/OFF Switch
Used to change fan cooling ON/OFF setting.
START Switch
Used to start operation.
STOP Switch
Used to stop operation.
PROGM Display
The display indicates the present program.
LID
CLOSE
STERI/DISSOL
HEAT
FAN
FUNC
.
SET
PROGM1
WARM
EXHT.
EXHT.
POWER
TEMP
2 3
℃
AGAR
LIQ.
SOLID
DISSOL
MPa
Hr/min.
DRAIN BOTTLE
℃
START
STOP
LID
OPEN
ON
OFF

 





 





 


－8－
Chapter 2. Installation
●If the equipment is installed in a place which is 800m or higher than sea level (i.e. under low
pressure in mountainous areas), the settings must be changed. In this case, be sure to contact
our authorized distributor in your region. Do not use the equipment before changing.
●When relocating the product, close the lid so that it will not move, and then remove the drain
bottle and power cord.
1. Installation instructions

Avoid installing the equipment in a place where its
body may be exposed to water or chemicals, or
where corrosive and explosive gases may be
produced neareby.

Avoid placing the equipment directly under a fire
detector. If you open the lid immediately after
completion of operation, steam comes out of the
working c hamber, and may activate the detector.

Arrange the equipment with a clearance of 10 cm
or wider on the right side and 12 cm or wider on
the rear side to prevent the vent hole from
being blocked.
10cm or more

Avoid an installation place which is subject to impact
or vibration.

Place the unit in a level, firm place.

Avoid installing in a place which is subjected to a room
temperature of 5
℃
or below or 35
℃
or above.

Avoid installing the equiopment in a place which is
exposed to hight humidity, direct sunlight or much
dust.
0.2
0.3
0.40
0.1
0.2
0.3
0.40
0.1
0.2
0.3
0.4
0
0.1
10cm or more
12cm or more
IMPORTANT
!

－9－
2. Installation Procedure
Pour water into the cooling tank.
・Remove the cap from the water filler port at the
back of the unit, and pour water through the
water filler port by using a hose, etc. up to the
center of the level confirmation window at the
side of the unit.
After water supply, return the cap back to the
original position.
(Hoses, etc. are not provided. Please prepare
them by yourself.)
Put casters on the caster stoppers so that the
unit will not move.
・Place two casters at a position more than 64 cm
away from the wall, and push the unit so that the
front casters will be on the stoppers.
・Be sure to set the caster stoppers, otherwise the
full water function of the drain bottle may not be
activated.
Connect the power cord to the rated power supply.
・Be sure to connect the grounding wire.
●Do not forcibly bend, twist, tie, or extend the power cord. Do not place heavy objects on the cord.
A damaged cord or exposed wire may cause fire or electric shock.
●Never connect the power cord to a power supply with a voltage other than the rated voltage.
Connection to such a power supply may cause fire or electric shock.
●If not plugging the sterilizer into a grounded socket, ground the equipment separately before
connecting it to the power source.
●Never ground to a gas pipe or vinyl chloride water service pipe.
・ Connect the unit to a power unit of as below and to ground the Green/Yellow grounding wires.
・ AC100V: 20Amp or more, AC110V: 19Amp or more, AC120V: 17Amp or more,
・ AC220V: 10Amp or more, AC230 and AC240V : 9Amp or more.
CONNECT TO RATED VOLTAGE
Black
White
Green/Yellow
Connect
to ground
Water filler port
Cap
Center
Waterlevelcheckwindow
64 cm or more
Caster stopper
CAUTION
!

－10－
Turn ON the main power switch.
・Raise the lever of the main power switch at the right side of the unit.
Raise.
Main power switch
Referring to “Chapter 3, Operation Method”, open the lid and take out the accessories.
Place the bottom plate in the chamber.
Attach the drain bottle.
・Check that there is no foreign substance or irregularity on the floor where the drain bottle is
to be set.
Drain bottle

－11－
Chapter 3. Operation Method
Basic Operation Method
Turn “ON” Power Switch ―――――― See "1. Turning ON Power Switch” on page 7.
󰁎
Open the lid
󰁎
――――― See "2. Pouring Water" on page 7.
Pour water
󰁎
Load substance ―――――― See "3. Loading Substance" on page 8.
󰁎
Check and select cycle
See "4. Selection of Cycle and Program" on page 9.
󰁎
Check and change set
values
See "5. Changing Set Values" on page 11.
󰁎
󰁎
(When starting operation by Turn-on Timer)
See “7. Checking and Setting of Turn-on Timer”
on page 16
Start operation
See "8. Starting Operation" on page 18.
󰁎󰁎
Check if operation is
completed
󰁎
Open the lid ――――― See "9. Unloading" on page 19.
󰁎
Unload substance
󰁎
Turn “OFF” Power Switch ―――――― See "10. After Completion of Operation" on page 19.
Set Turn-on Timer

－12－
1. Power
Press the "POWER" switch on the operation panel.
・The light comes on, the preset value is displayed, and the unit enters the standby state.
・When none of the operation switches is touched for 10 minutes, the power saving function is
activated, the display light goes out, and a dot blinks on the digital display. Press any switch
other than the "LID OPEN" switch, and the unit returns to the standby state.
●When the power supply is interrupted or the main power switch is turned OFF, the temperature
and time set for the previous operation will appear.
2. Pouring Water
●When the sterilization time is extended or a large quantity of cloth is put in, pour a larger quantity
of water than specified.
Press the "LID OPEN" switch.
・The lid will open automatically.
Pour water until water rises through the hole at the
center of the drain-board.
・When there is no water at all, pour 3 liters of water.
Supply of water is necessary also for the dissolution load.
󰁅
󰁅󰁅
󰁅 NOTE
IMPORTANT
!
STERI/DISSOL
HEAT EXHT.
POWER
PROGM1
TEMP
2 3
℃
MPa
Hr/min.

－13－
3. Loading Substance
●Be careful not to pinch hands when closing the lid.
●Close the lid after confirming that no foreign matter is adhering to the section contacting the lid
gasket. Foreign matter in this section may cause vapor leaks.
●When using a waste processing bag or other kind of bag and disinfecting, place the bag in the
metal mesh holder and insert it into the working chamber. Using the bag “as is” can cause
excessive temperatures, pressures, lack-of -water, etc.
●Be sure to use the drain-board.
●The section of the inlet of the chamber, which is to be in contact with the gasket, is important for
gas sealing. Exercise care so as not to damage the section by hitting it with a loaded substrate.
●Do not put in any substance larger than the inner dimensions of the chamber.
Put a substance (dissolved material)
in the chamber.
●When sterilizing an empty deep container, lay the container on its side in the chamber so that it
will be permeated with steam. An upright position may cause insufficient sterilization.
●If a waste disposal bag is used in sterilization, open the bag far enough that the bag is not in
contact with the inside surface of the chamber. Insufficient sterilization may be caused if the bag
is closed during sterilization. When the bag is opened excessively, steam is prevented from
circulating in the chamber. This may also result in insufficient sterilization.
●Do not pile specimens on top of one another. When the chamber is overly packed, steam fails to
penetrate to all points, resulting in incomplete sterilization.
●In sterilizing liquids such as chemicals and media, pay attention to the quantity of the liquid in
relation to its container. For an Erlenmeyer flask, the amount of chemical should be approx. 3/4 of
the capacity of the container; for a test tube, the appropriate quantity of chemical is approx. half
of the capacity of the container. Too much chemical may result in overflow from the container
during the temperature rising or cooling process.
●Use container caps that are loose fitting and allow the passage of air. Containers may break if
venting is not possible.
●
In the case of dissolution of agar media, its quantity should be 2 liters or less per container.
Two liters or more of agar medium may not be completely dissolved.
●Use the DURHAM TEST TUBE (Sample tube) with 6mm caliber or more. At the DURHAM TEST
TUBE (Sample tube) with less than 6 mm caliber, air bubble sometimes remains. Delaying of
air release time may allow no air bubbles to be left. Change the air release time, referring to “6.
Changing Function Setting.”
CAUTION
IMPORTANT
󰁅
󰁅󰁅
󰁅 NOTE
!
!

－14－
4. Selection of Cycle and Program
●Select a cycle according to the usage.
Press the cycle switch (LIQ., AGAR, SOLID, or
DISSOL).
・Every time the switch is pressed, the program
changes 2, 3, 1, ... repeatedly.
Cycle Application
Liquid Sterilization of water, media, solid reagent, liquid reagent, liquid chemical, and other
liquid that can withstand high-temperature high-pressure steam.
Agar Sterilization of agar media (to be warmed so that it will not solidify after sterilization)
Solid Sterilization of glass, ceramics, metal, rubber, and other solid equipment that can
withstand high-temperature high-pressure steam and sudden decompression
during exhaust
Dissolution Dissolution of media
Initial set value (for all 3 programs)
Display of cycle Sterilizati
on temp.
Sterilizati
on time
Exhaust
level
Fan
cooling
Warming
temp.
HEAT →STERI →
EXHT./COOL 121℃20 min. 0 OFF
Liquid cycle
STERI/DISSOL
HEAT
FAN
PROGM1
WARM
EXHT.
EXHT.
TEMP
2 3
℃
AGAR
LIQ.
SOLID
DISSOL
MPa
Hr/min.
ON
OFF
Initial set value (for all 3 programs)
Display of cycle Sterilizati
on temp.
Sterilizati
on time
Exhaust
level
Fan
cooling
Warming
temp.
HEAT →STERI →
EXHT./ COOL →WARM 121℃20 min. 0 OFF 50℃
Agar cycle
STERI/DISSOL
HEAT
FAN
PROGM1
WARM
EXHT.
EXHT.
TEMP
2 3
℃
AGAR
LIQ.
SOLID
DISSOL
MPa
Hr/min.
ON
OFF
AGAR
LIQ.
SOLID
DISSOL

－15－
Initial set value (for all 3 programs)
Display of cycle Sterilizati
on temp.
Sterilizati
on time
Exhaust
level
Fan
cooling
Warming
temp.
HEAT →STERI →
EXHT./COOL 121℃20 min. 3 (fixed) ON
(fixed)
Solid cycle
STERI/DISSOL
HEAT
FAN
PROGM1
WARM
EXHT.
EXHT.
TEMP
2 3
℃
AGAR
LIQ.
SOLID
DISSOL
MPa
Hr/min.
ON
OFF
Initial set value (for all 3 programs)
Display of cycle Sterilizati
on temp.
Sterilizati
on time
Exhaust
level
Fan
cooling
Warming
temp.
HEAT →DISSOL →
COOL →WARM 100℃10 min. OFF 50℃
Dissolution
cycle
STERI/DISSOL
HEAT
FAN
PROGM1
WARM
EXHT.
EXHT.
TEMP
2 3
℃
AGAR
LIQ.
SOLID
DISSOL
MPa
Hr/min.
ON
OFF

－16－
5. Changing Set Values (Registering of Values by Customer)
●When power supply is interrupted or the main power switch is turned OFF before operation with
the preset temperature and time, the preset values will return to the original values.
Select the cycle for which the setting is to be changed, select the program number under which the
set values are to be saved, and then change the setting as shown below.
●Sterilization temperature, sterilization time, warming temperature, dissolution
temperature, and dissolution time
Set values cannot be changed during operation.
Press the "SET" switch.
・The sterilization (dissolution) set temperature
display blinks, permitting setting change.
When changing the setting other than the
sterilization (dissolution) temperature, press
the "SET" switch further and select the setting
item to change.
・Every time the switch is pressed, the changeable setting items change repeatedly as shown
below.
Switch input
󰁏󰁏󰁏
Liquid cycle Sterilization
temperature Sterilization time
Agar cycle Sterilization
temperature Sterilization time Warming temperature
Solid cycle Sterilization
temperature Sterilization time
Dissolution
cycle Dissolution
temperature Dissolution time Warming temperature
Press the set value increase/decrease switch (󰁄
󰁄󰁄
󰁄󰁎
󰁎󰁎
󰁎) to change the value.
・Every time the switch is pressed:
The stylization temperature increases or decreases within the range of 5-135℃at every ℃.
The sterilization time increases or decreases within the range of 1 min.-5 hr at every minute.
The dissolution temperature increases or decreases within the range of 60-100℃at every ℃.
The dissolution time increases or decreases within the range of 1 min.-1 hr at every minute.
The warming temperature increases or decreases within the range of 45-60℃at every ℃.
・Keep pressing the switch, and the value increases at the rate of 10 units, returning to the
lower (upper) limit when the value exceeds the upper (lower) limit.
Leave the switch untouched for 5 seconds, and the blinking display will remain lit,
completing the setting.
󰁅
󰁅󰁅
󰁅 NOTE
SET SET SET
FAN
FUNC.
SET
PROGM1
EXHT.
TEMP
2 3
℃
MPa
Hr/min.
ON
OFF

－17－
●For sterilization of liquid, set a sterilization time longer than desired, taking delay time into
account and referring to the table below.
 In case there is 3 liters of water in a flask, it takes nearly 30 minutes (delay time) for
temperature of water in the container to reach a set sterilization temperature after
temperature in the chamber reaches the set value. You should set a sterilization time
30 minutes longer than desired to cope with this delay of time. Therefore, the set
sterilization is 50 minutes:
 Set sterilization time (50 minutes)
= Delay time (30 minutes) + desired sterilization time (20 minutes)
HG-50 Reference Values of Delay Time
(per Flask)
Liquid Volume Delay Time
3 liters 30 minutes
2 liters 25 minutes
1 liter 20 minutes
500 cc 15 minutes
●If steam is abruptly exhausted after sterilization
of liquid, the liquid may gush out. To prevent
this, set the opening of the exhaust valve
(Exhaust % ) to a small value to gradually
exhaust. Or, set that to 0 % (natural cooling).
●When used with a sterilization can, it takes
several hours for the temperature in waste disposal bag to reach the set temperature after the
temperature in the chamber (displayed temperature) reaches the set value (time lag).
If there is approx. 300 ~ 500 milliliters of water in the waste disposal bag, steam is generated in
the bag and drives the air out. This will significantly reduce the time lag at the time of
temperature rise. Refer to the table below and take this time lag into account when setting the
fertilization time.
The delay time will be shortened when a perforated sterilization can is used.
HG-50 Reference Values for Time Lag in Bag
Water in Bag Time Lag
Not poured 206 minutes
Poured 48 minutes
Model: HG-50
Case: A large number of φ15 x 100 test tubes
placed in a waste disposal bag.
●For dissolution of coagulated agar medium, set an appropriate dissolution temperature and time,
referring to the table below.
HG-50 Reference Values (per Flask)
Quantity of Liquid Dissolution Temperature Dissolution Time
2 liters 60 minutes
1 liter 45 minutes
500 cc
100℃25 minutes
󰁅
󰁅󰁅
󰁅 NOTE
40
60
80
100
120
1
2
0
(Temp er atur e)
(Time)
S et ste riliz at io n tim e
Delay tim e Ste rilizat ion tim e
Tem pe rature of wate r
Temp eratu re
HG- 50 Delay Tim e Reference Data
in 3 liter container
temp.
Room
in working c hamber

－18－
●Exhaust level
The exhaust level can be changed from level 0 to level 3 in the liquid and agar cycles.
It can be changed also during operation.
Press the "

" switch to increase the exhaust level, and press the "

" switch to decrease
the level.
EXHT.
●Sudden exhaust after sterilization of liquid may cause the liquid to spill. Set a lower exhaust
level for fine exhaust, or set the level 0 (natural cooling).
●Continuous operation at the exhaust level of 3 may cause steam to come out of the exhaust port
of the drain bottle. In that case, lower the exhaust level, except for the solid cycle, and turn ON
the fan cooling function.
●Fan cooling ON/OFF
Except the solid cycle, fan cooling ON/OFF can be changed. It can be changed also during
operation.
Press the "Fan cooling ON/OFF" switch.
・When the fan display remains lit, the fan begins to rotate after sterilization.
FAN
󰦬󰦫
󰦬ＦＦ
󰁅
󰁅󰁅
󰁅 NOTE

－19－
6. Changing Function Setting
●When power supply is interrupted or the main power switch is turned OFF before operation with
the preset temperature and time, the preset values will return to the original values.
Select the cycle for which the setting is to be changed, select the program number under which the
set values are to be saved, and then change the setting as shown below.
●Air release time
The initial setting for air release is set at 4 min. after 97℃detection. If air bubbles remain in a
Durham tube and the like of less than 6 mm in inner diameter, extension of the air release time
may eliminate remaining air bubbles. Extension of the air release time may eliminate remaining
air bubbles also when a large quantity of cloth is put in the chamber.
The time can be set for each cycle and program number. The set value cannot be changed
during operation.
Press the "FUNC." switch two times in the standby state.
・"F2" will appear on the digital display, and the air release set time display will blink, entering
the changeable state.
FAN
FUNC.
SET
PROGM1
EXHT.
TEMP
2 3
℃
MPa
Hr/min.
ON
OFF
฀
Press the set value increase/decrease switch (󰁄
󰁄󰁄
󰁄󰁎
󰁎󰁎
󰁎) to change the value.
・Every time the switch is pressed, the time increases (decreases) within the range of 3-9 min.
at every minute.
・When the set value exceeds the upper (lower) limit, it will return to the lower (upper) limit.
Leave the switch untouched for 5 seconds, and the display will return to the standby
state, completing the setting.
󰁅
󰁅󰁅
󰁅 NOTE