Agilent Technologies InfinityLab LC Series User manual

1290 Infinity II
Preparative Open-Bed Fraction Collector
Agilent InfinityLab LC Series
User Manual

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
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Document No: SD-29000163 Rev. F
EDITION 11/2019
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1290 Infinity II Preparative
Open-Bed Fraction Collector
User Manual

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 3
In This Guide
This manual contains technical reference information about the Agilent 1290
Infinity II Preparative Open-Bed Fraction Collector (G7159B).
1Introduction
This chapter gives an introduction to the module and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical and
performance specifications.
3 Using the Fraction Collector
This chapter explains the essential operational parameters of the module.
4 Preparing the Fraction Collector
This chapter explains the operational parameters of the module.
5Troubleshootingand Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic features
and the different user interfaces.
6ErrorInformation
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.
7 Maintenance
This chapter describes the maintenance of the module.
8 Parts for Maintenance and Repair
This chapter provides information on parts for maintenance and repair.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 4
9IdentifyingCables
This chapter provides information on cables used with the module.
10 Hardware Information
This chapter describes the module in more detail on hardware and electronics.
11 Appendix
This chapter provides additional information on safety, legal, and web.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 5
Contents
1Introduction8
Intended Use 9
Product Description 10
Features 11
Overview of the Module 12
Fraction Collector Principle 13
Leak and Waste Concept 14
2 Site Requirements and Specifications 16
Site Requirements 17
Physical Specifications 22
Performance Specifications 23
3 Using the Fraction Collector 27
Warnings and Cautions 28
Solvent Information 29
Turn on/off 36
Status Indicators 37
Drawer Status Indicator 38
Exchange Drawers 39
Exchange Containers 41
Replace Inlet/Waste Tubings 43
Configuration and Operation of the Open Bed Fraction Collector 47
Prepare the Module for Transportation 61

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 6
4 Preparing the Fraction Collector 63
Best Practices 64
Capillary Color Coding Guide 65
Swage Fittings 66
Setting up the Fraction Collector with the Instrument Control Interface 68
Method Parameter Settings 74
Pooling 82
5 Troubleshooting and Diagnostics 83
User Interfaces 84
Agilent Lab Advisor Software 85
6ErrorInformation86
What Are Error Messages 87
General Error Messages 88
7 Maintenance 95
Introduction to Maintenance 96
Warnings and Cautions 97
Cleaning the Module 99
Overview of Maintenance 100
Install and Remove the Top Fume Hood 101
Clean the Leak Pan 103
Replace the Dripping Adapter for the Fraction Valve 104
Replace the Module Firmware 106
8 Parts for Maintenance and Repair 107
Supported Containers 108
List of Recommended Fraction Tubes 109
Fraction Collector Accessory Kit 110
Tubing Kits 111
Top Fume Hood Kit 115

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 7
9IdentifyingCables116
Cable Overview 117
Analog Cables 119
Remote Cables 121
CAN/LAN Cables 125
RS-232 Cables 126
USB 127
10 Hardware Information 128
Firmware Description 129
Electrical Connections 132
Interfaces 134
Setting the 6-bit Configuration Switch 141
Early Maintenance Feedback 145
Instrument Layout 146
11 Appendix 147
General Safety Information 148
Waste Electrical and Electronic Equipment (WEEE) Directive 154
Radio Interference 155
Sound Emission 156
Agilent Technologies on Internet 157

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 8
1Introduction
Intended Use 9
Product Description 10
Features 11
Overview of the Module 12
Fraction Collector Principle 13
Leak and Waste Concept 14
Leak Sensor 15
This chapter gives an introduction to the module and an instrument overview.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 9
1Introduction
Intended Use
Intended Use
The 1290 Infinity II Preparative Open-Bed Fraction Collector is designed to be
used in combination with other InfinityLab LC modules in a preparative-scale
HPLC system to separate and purify inorganic or organic compounds via
reversed-phase chromatography applications. The module can collect fractions
of desired volume for sample purification or for later analysis.
The product is intended to be used by professional laboratory users.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 10
1Introduction
Product Description
Product Description
The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector is the ideal
solution that automatically manages high-capacity fraction collection of purified
peaks for semi-preparative and preparative scale purification. Collect milligrams
to multi-gram quantities of sample intuitive with ease. The Agilent 1290 Infinity II
Preparative Open-Bed Fraction Collector features handling a wide variety of
collection vessels with ease and capacity of up to 5.9 L by using 150 x 25 mm (L
x OD). The containers use identification tags to automatically detect the vessel
dimensions. With a novel robotic technology, Y-, Z-, theta-axis probe functionality
minimizes sample carryover between the fractions during movements between
the vessels. The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector
is optimized for flow rates up to 200 mL/min, and can be easily integrated with
any Agilent solvent delivery module, autosampler, or detector. For
high-throughput applications, you can combine up to three fraction collectors in a
single LC system. Scale up your fraction capacity based on your demand up to a
maximum of 1296 fractions.
Figure 1 Overview of the open-bed fraction collector
Status indicator
Container
Drawer
Power switch