Agilent Technologies InfinityLab LC Series User manual

1290 Infinity II
Preparative Open-Bed Fraction Collector
Agilent InfinityLab LC Series
User Manual

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
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Document No: SD-29000163 Rev. F
EDITION 11/2019
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1290 Infinity II Preparative
Open-Bed Fraction Collector
User Manual

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 3
In This Guide
This manual contains technical reference information about the Agilent 1290
Infinity II Preparative Open-Bed Fraction Collector (G7159B).
1Introduction
This chapter gives an introduction to the module and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical and
performance specifications.
3 Using the Fraction Collector
This chapter explains the essential operational parameters of the module.
4 Preparing the Fraction Collector
This chapter explains the operational parameters of the module.
5Troubleshootingand Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic features
and the different user interfaces.
6ErrorInformation
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.
7 Maintenance
This chapter describes the maintenance of the module.
8 Parts for Maintenance and Repair
This chapter provides information on parts for maintenance and repair.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 4
9IdentifyingCables
This chapter provides information on cables used with the module.
10 Hardware Information
This chapter describes the module in more detail on hardware and electronics.
11 Appendix
This chapter provides additional information on safety, legal, and web.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 5
Contents
1Introduction8
Intended Use 9
Product Description 10
Features 11
Overview of the Module 12
Fraction Collector Principle 13
Leak and Waste Concept 14
2 Site Requirements and Specifications 16
Site Requirements 17
Physical Specifications 22
Performance Specifications 23
3 Using the Fraction Collector 27
Warnings and Cautions 28
Solvent Information 29
Turn on/off 36
Status Indicators 37
Drawer Status Indicator 38
Exchange Drawers 39
Exchange Containers 41
Replace Inlet/Waste Tubings 43
Configuration and Operation of the Open Bed Fraction Collector 47
Prepare the Module for Transportation 61

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 6
4 Preparing the Fraction Collector 63
Best Practices 64
Capillary Color Coding Guide 65
Swage Fittings 66
Setting up the Fraction Collector with the Instrument Control Interface 68
Method Parameter Settings 74
Pooling 82
5 Troubleshooting and Diagnostics 83
User Interfaces 84
Agilent Lab Advisor Software 85
6ErrorInformation86
What Are Error Messages 87
General Error Messages 88
7 Maintenance 95
Introduction to Maintenance 96
Warnings and Cautions 97
Cleaning the Module 99
Overview of Maintenance 100
Install and Remove the Top Fume Hood 101
Clean the Leak Pan 103
Replace the Dripping Adapter for the Fraction Valve 104
Replace the Module Firmware 106
8 Parts for Maintenance and Repair 107
Supported Containers 108
List of Recommended Fraction Tubes 109
Fraction Collector Accessory Kit 110
Tubing Kits 111
Top Fume Hood Kit 115

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 7
9IdentifyingCables116
Cable Overview 117
Analog Cables 119
Remote Cables 121
CAN/LAN Cables 125
RS-232 Cables 126
USB 127
10 Hardware Information 128
Firmware Description 129
Electrical Connections 132
Interfaces 134
Setting the 6-bit Configuration Switch 141
Early Maintenance Feedback 145
Instrument Layout 146
11 Appendix 147
General Safety Information 148
Waste Electrical and Electronic Equipment (WEEE) Directive 154
Radio Interference 155
Sound Emission 156
Agilent Technologies on Internet 157

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 8
1Introduction
Intended Use 9
Product Description 10
Features 11
Overview of the Module 12
Fraction Collector Principle 13
Leak and Waste Concept 14
Leak Sensor 15
This chapter gives an introduction to the module and an instrument overview.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 9
1Introduction
Intended Use
Intended Use
The 1290 Infinity II Preparative Open-Bed Fraction Collector is designed to be
used in combination with other InfinityLab LC modules in a preparative-scale
HPLC system to separate and purify inorganic or organic compounds via
reversed-phase chromatography applications. The module can collect fractions
of desired volume for sample purification or for later analysis.
The product is intended to be used by professional laboratory users.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 10
1Introduction
Product Description
Product Description
The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector is the ideal
solution that automatically manages high-capacity fraction collection of purified
peaks for semi-preparative and preparative scale purification. Collect milligrams
to multi-gram quantities of sample intuitive with ease. The Agilent 1290 Infinity II
Preparative Open-Bed Fraction Collector features handling a wide variety of
collection vessels with ease and capacity of up to 5.9 L by using 150 x 25 mm (L
x OD). The containers use identification tags to automatically detect the vessel
dimensions. With a novel robotic technology, Y-, Z-, theta-axis probe functionality
minimizes sample carryover between the fractions during movements between
the vessels. The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector
is optimized for flow rates up to 200 mL/min, and can be easily integrated with
any Agilent solvent delivery module, autosampler, or detector. For
high-throughput applications, you can combine up to three fraction collectors in a
single LC system. Scale up your fraction capacity based on your demand up to a
maximum of 1296 fractions.
Figure 1 Overview of the open-bed fraction collector
Status indicator
Container
Drawer
Power switch

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 11
1Introduction
Features
Features
•Maximize your flexibility – Choose how much you want to collect, whether you
prefer a large number of fractions which enables collection of up to 432
fraction in 150 x 12 mm (L x OD) vessels, or you prefer large volumes, or any
combination of formats. Fraction Containers are available in eight formats,
and you mix them to accommodate the different vessel ODs.
•High capacity – Using one type of drawer and offering six different type of
containers, the Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector
can collect a maximum of 5.9 L.
•Scalable collection volumes – The Agilent 1290 Infinity II Preparative
Open-Bed Fraction Collector enhances flexibility by providing differently
optimized tubing kits. Optimized for collections up to 200 mL/min.
•Accurate collection modes – real time peak or mass detection algorithms
allow to achieve accurate, reproducible fraction detection every time.
•Lowest delay volumes - Optimized tubing and unique delay sensor for
accurate collection minimize peak dispersion.
•Low carryover - The Agilent 1290 Infinity II Preparative Open-Bed Fraction
Collector is designed for low carryover due to extremely fast Y-, Z-, and
Theta-axis movement, thus minimizing sample loss during movements from
vessel-to-vessel.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 12
1Introduction
Overview of the Module
Overview of the Module
The Fraction Collector transport mechanism uses a cartesian robot. The Y drive
together with the Theta drive optimize the positioning for the fraction containers.
The robot moves above a position and the diverter valve switches to collect in the
according collection position.
All axes of the transport mechanism are driven by very fast BLDC motors. Optical
encoders ensure the correct operation of the movement.
The entire flow path including the diverter valve and delay sensor are always
flushed by the mobile phase for minimum internal carry-over.
To reduce carry-over further, you have the possibility to rinse the diverter valve on
a rinse port. The bottle containing the mobile phase for the wash procedure will
be located in the solvent bottle cabinet. The rinse waste produced during this
operation is channeled safely away through a waste drain.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 13
1Introduction
Fraction Collector Principle
Fraction Collector Principle
The movements of the Fraction Collector components during the sequence are
monitored continuously by the Fraction Collector processor. The processor
defines specific time windows and mechanical ranges for each movement. If a
specific step of the sequence is not completed successfully, an error message is
generated.
The standard fractioning sequence occurs in the following order:
1The Fraction Collector starts always from the home position.
2When the sample is injected, the fraction probe with diverter valve moves to
the required position.
3When the trigger is given by the detector, the diverter valve opens to collect
the fraction.
4When the trigger is given by the detector, the diverter valve closes and the arm
moves to the next fraction position or back to the home position if this
function is chosen in the CDS.
Fractioning Sequence
Before the start of the sequence, and during an analysis, the diverter valve is in
the fraction start position. In this position, the mobile phase flows through the
diverter valve towards waste.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 14
1Introduction
Leak and Waste Concept
Leak and Waste Concept
1Rinse Port 2Drain Outlet

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 15
1Introduction
Leak and Waste Concept
Leak Sensor
Figure 2 Position of leak tray and leak sensor
Leak tray
Leak sensor
CAUTION
Solvent incompatibility
The solvent DMF (dimethyl formamide) leads to corrosion of the leak sensor.
The material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible
with DMF.
Do not use DMF as mobile phase.
Check the leak sensor regularly for corrosion.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 16
2 Site Requirements and Specifications
Site Requirements 17
Exhaust and Vapour Management 20
Waste Management 21
Physical Specifications 22
Performance Specifications 23
This chapter provides information on environmental requirements, physical and
performance specifications.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 17
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Considerations
The module power supply has wide ranging capability. It accepts any line voltage
in the range described in Table 1 on page 22. Consequently there is no voltage
selector in the rear of the module. There are also no externally accessible fuses,
because automatic electronic fuses are implemented in the power supply.
WARNING
Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than
specified.
Connect your instrument to the specified line voltage only.
WARNING
Electrical shock hazard
The module is partially energized when switched off, as long as the power
cord is plugged in.
The cover protects users from personal injuries, for example electrical
shock.
Do not open the cover.
Do not operate the instrument and disconnect the power cable in case
the cover has any signs of damage.
Contact Agilent for support and request an instrument repair service.
WARNING
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument
from the power line at any time.
Make sure the power connector of the instrument can be easily reached
and unplugged.
Provide sufficient space behind the power socket of the instrument to
unplug the cable.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 18
2 Site Requirements and Specifications
Site Requirements
Power Cords
Country-specific power cords are available for the module. The female end of all
power cords is identical. It plugs into the power-input socket at the rear. The male
end of each power cord is different and designed to match the wall socket of a
particular country or region.
Agilent makes sure that your instrument is shipped with the power cord that is
suitable for your particular country or region.
WARNING
Unintended use of power cords
Using power cords for unintended purposes can lead to personal injury or
damage of electronic equipment.
Never use a power cord other than the one that Agilent shipped with this
instrument.
Never use the power cords that Agilent Technologies supplies with this
instrument for any other equipment.
Never use cables other than the ones supplied by Agilent Technologies
to ensure proper functionality and compliance with safety or EMC
regulations.
WARNING
Absence of ground connection
The absence of ground connection can lead to electric shock or short
circuit.
Never operate your instrumentation from a power outlet that has no
ground connection.
WARNING
Electrical shock hazard
Solvents may damage electrical cables.
Prevent electrical cables from getting in contact with solvents.
Exchange electrical cables after contact with solvents.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 19
2 Site Requirements and Specifications
Site Requirements
Bench Space
The module dimensions and weight (see Table 1 on page 22) allow you to place
the module on almost any desk or laboratory bench. It needs an additional
2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches)
in the rear for air circulation and electric connections.
If the bench shall carry a complete HPLC system, make sure that the bench is
designed to bear the weight of all modules.
The module should be operated in a horizontal position.
Condensation
CAUTION
Condensation within the module
Condensation can damage the system electronics.
Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
If your module was shipped in cold weather, leave it in its box and allow it to
warm slowly to room temperature to avoid condensation.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 20
2 Site Requirements and Specifications
Site Requirements
Exhaust and Vapour Management
You can operate the module in three different scenarios:
1Under laboratory fume hood:
•Ensure adequate fume hood ventilation: the recommended ventilation
speed is 200 m
3
/h with closed sash window and 490 m
3
/h with open sash
window.
2On laboratory desk with module-specific fume hood:
•Connect the exhaust tube to the laboratory’s venting system.
•Ensure a minimum air flow of 6 L/s through the exhaust tube.
•Ensure an underpressure in the venting system.
3Without fume hood:
•Ensure adequate laboratory ventilation: the recommended air exchange
rate is 10 times per hour, the minimum required air exchange rate is 8
times per hour.
For details, refer to the Site Preparation Checklist.
WARNING
Hazardous or toxic fumes and vapors
Organic solvent vapors can reach harmful, toxic, or explosive
concentrations when they are not removed sufficiently with an appropriate
venting system.
Ensure that your venting/air exchange solution meets the requirements
specified here for your relevant operation scenario.
Ensure that the solvent temperature is always kept below the specified
limit.